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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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Re: Ask Quick Questions, Get Quick Answers [Re: RoscoeReturns] * 1
    #29458894 -

Ok phew, thank you, that's reassuring.

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Re: Ask Quick Questions, Get Quick Answers [Re: Infinite Monkeys]
    #29458945 -

I was wondering should I harvest when most of the caps turn golden the golden ones have broken their veils. I was going to wait till tomorrow since only 2 have turned golden so far. They also turning blue slighty when they clustered together from touching each other.


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I am the master of my fate,
      I am the captain of my soul.

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Re: Ask Quick Questions, Get Quick Answers [Re: CaptainTrips420]
    #29458998 -

Dealers choice.

I personally would get the two biggins and then get the rest later in the day.

But I hate spore dumps.


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Favorite Teks

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Re: Ask Quick Questions, Get Quick Answers [Re: Splittinghairs]
    #29459001 -

I dont think you'll see spores for a couple day still


--------------------
"Remember, kids, the difference between science and screwing around is writing it down" -adam savage
Flowchart for Recommended plan of action.
Learn the tried and true way to grow mushrooms
Use the Damn search engine
After you know what you're doing, take a break 
Pick a book, Make some chips!
Josex said:Don't take the site seriously bro, ain't worth it.
 

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Re: Ask Quick Questions, Get Quick Answers [Re: tryptkaloids]
    #29459015 -

The veil tor mostly from rubbing on the lower cap I think the largest ones haven't torn yet but are close its strange they turn golden brown and have a blue tint in the viel.

I also can't pick them since they clustered together I think ill just wait. I didn't really notice less growth from spore drop in chestnut mushrooms the tub I harvest early grew about the same amount on the second flush as the tub that got spores everywhere.


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I am the master of my fate,
      I am the captain of my soul.

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Re: Ask Quick Questions, Get Quick Answers [Re: CaptainTrips420]
    #29459112 -

How long does everyone's agar plate take to fully colonize? How long for 50% plate colonization for cubes? I think my golden teacher culture is very slow.

It's been 3 days since doing an A2A transfer and it's only now that the wedge is fuzzy, yet to extend outwards. There hasn't been any rhizo growth. I used the 50ml to 1g of agar and LME recipe. Is my culture's genetics just shitty?

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Re: Ask Quick Questions, Get Quick Answers [Re: shroomerhumor]
    #29459146 -

You should be more than half colinzed in a week probably enough to use the whole plate. I get mostly colinzed plates in often about 10 days. I did 4 A2A transfers yesterday and one is already growing on the new agar.
That is only 24 hours old Tresure Coast. The others are slower you should try doing lots of transfers I get the furthest growth from the start at about 50% but you should see large growth in the next 7 days.


--------------------
I am the master of my fate,
      I am the captain of my soul.

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Re: Ask Quick Questions, Get Quick Answers [Re: CaptainTrips420]
    #29459154 -

Damn dude that is much faster than mine.


Backside of T2 plate inoculated 1-16-26. Slow but it's at least the cleanest transfer out of the bacterial LC..

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Re: Ask Quick Questions, Get Quick Answers [Re: shroomerhumor] * 3
    #29459165 -

shroomerhumor said:
How long does everyone's agar plate take to fully colonize? How long for 50% plate colonization for cubes? I think my golden teacher culture is very slow.

It's been 3 days since doing an A2A transfer and it's only now that the wedge is fuzzy, yet to extend outwards. There hasn't been any rhizo growth. I used the 50ml to 1g of agar and LME recipe. Is my culture's genetics just shitty?


For me, germinating sporeless golden teacher, the plates have moved pretty slow. They were in my kitchen at around 68 to 70° for the first week to 10 days and I was getting frustrated at the rate of growth. I moved them into a dedicated grow room with some temperature control at 75° and they took off pretty fast. All in all I think full colonization by 2 to 3 weeks is probably the rate that I experienced.

That's on LME agar, by the way. I plated my original cultures on New Year's Eve and I sent some of those to grain 2 days ago. You can take a look at the grow log in my journal to get an idea of timeline if it's helpful, but it's only my first grow.


--------------------
-Noku
Eskawata kaya waikiki
HHG
"Shake it like you would a newborn baby" - BeefExtreme

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Re: Ask Quick Questions, Get Quick Answers [Re: Nokumana]
    #29459243 -

Nokumana said:
shroomerhumor said:
How long does everyone's agar plate take to fully colonize? How long for 50% plate colonization for cubes? I think my golden teacher culture is very slow.

It's been 3 days since doing an A2A transfer and it's only now that the wedge is fuzzy, yet to extend outwards. There hasn't been any rhizo growth. I used the 50ml to 1g of agar and LME recipe. Is my culture's genetics just shitty?


For me, germinating sporeless golden teacher, the plates have moved pretty slow. They were in my kitchen at around 68 to 70° for the first week to 10 days and I was getting frustrated at the rate of growth. I moved them into a dedicated grow room with some temperature control at 75° and they took off pretty fast. All in all I think full colonization by 2 to 3 weeks is probably the rate that I experienced.

That's on LME agar, by the way. I plated my original cultures on New Year's Eve and I sent some of those to grain 2 days ago. You can take a look at the grow log in my journal to get an idea of timeline if it's helpful, but it's only my first grow.


I'll try keeping them at 75f. You have a great log going there, mine is very basic lol. Your GT growth rate and mine are basically the same. I'm at T2 for plates and inoculated one grain jar with the bacterial LC to learn how to spot bacterial contam in jars and who knows, I might be able to fruit it in-vitro. You and I are both on our first grow and I'm excited to see your progress. I'm betting you'll get fruit way before me.

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Re: Ask Quick Questions, Get Quick Answers [Re: shroomerhumor]
    #29459253 -

I do everything at 72F I had a heat mat that sometimes was 75-80F but would randomly be over 90F sometimes. It slowed the growth down but I find 72F great.

Im surprised the GT is so slow I thought it was a faster strain.

If you're really having trouble growing you can actually buy pre colinzed grain. Its pretty expensive about $40 for 32oz bag. I use a website that sells it but its pre-order only they ship after a couple weeks and make sure its clean spawn. Just search Google for Research Grain Archives and you should see it I can't source them directly through :shrug:


--------------------
I am the master of my fate,
      I am the captain of my soul.

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Re: Ask Quick Questions, Get Quick Answers [Re: CaptainTrips420] * 3
    #29459261 -

Well my t0 plates were bacterial but even my clean T2 plates are still going slow. I don't think I'll ever buy pre colonized grains especially when I have everything already. I'd rather do every step myself so the literal fruits of my labor feel earned :smile:

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Re: Ask Quick Questions, Get Quick Answers [Re: CaptainTrips420] * 3
    #29459295 -

CaptainTrips420 said:
I do everything at 72F I had a heat mat that sometimes was 75-80F but would randomly be over 90F sometimes. It slowed the growth down but I find 72F great.

Im surprised the GT is so slow I thought it was a faster strain.

If you're really having trouble growing you can actually buy pre colinzed grain. Its pretty expensive about $40 for 32oz bag. I use a website that sells it but its pre-order only they ship after a couple weeks and make sure its clean spawn. Just search Google for Research Grain Archives and you should see it I can't source them directly through :shrug:


Thanks for this, captain. Just to be clear... I'm not doing this to grow quickly. I'm doing this to grow right. For me agar has turned into its own separate micro hobby. It's great for dialing in sterile technique in an SAB, and I love taking transfers to come up with clean plates. My t1 plates are so beautiful looking and now the t2s are starting to grow. I'll post photo soon on my log. I'm just really proud looking at them. They're like my babies. And when I find contamination I'm like oh shit I have to make a move now I have to fix this plate quickly and rescue my babies!

ShroomerHumor, I'm not sure that I can expect to be fruiting any sooner than you to be honest. I only just went to grain with a couple of plates a couple of days ago and so far I'm not seeing much going on. Impatience creeping into the process again. Now that I feel like I've got a good start at agar it's time for me to get my grain skills dialed in. It'll be fun to track each other's progress. At the end we can exchange genetics! What are you growing?


--------------------
-Noku
Eskawata kaya waikiki
HHG
"Shake it like you would a newborn baby" - BeefExtreme

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Re: Ask Quick Questions, Get Quick Answers [Re: Nokumana]
    #29459475 -

I got some jars that are colonized for like 6 weeks now. And I finally have time to spawn them.

But I noticed some water at the bottom. Not much but noticeable amount.  Is this ok? Can I still spawn normally ?


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Agar stuff

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Re: Ask Quick Questions, Get Quick Answers [Re: MycoKyle]
    #29459482 -

Probably just condensation that made its way to the bottom of the jar. Unless it smells weird when you open the jar, I say send it.

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Re: Ask Quick Questions, Get Quick Answers [Re: AstroSporeFiend] * 1
    #29459517 -

Pooling liquid isnt a great sign, but :whathesaid:


--------------------
"Remember, kids, the difference between science and screwing around is writing it down" -adam savage
Flowchart for Recommended plan of action.
Learn the tried and true way to grow mushrooms
Use the Damn search engine
After you know what you're doing, take a break 
Pick a book, Make some chips!
Josex said:Don't take the site seriously bro, ain't worth it.
 

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Re: Ask Quick Questions, Get Quick Answers [Re: tryptkaloids]
    #29459696 -

Hey does this look like contamination? its a different growth than the agar and it hasn't ever gotten a break and shake so its ether contamination or little bits of agar that fell off when I shook it down. It just seems a different color possibley cobweb mold.


--------------------
I am the master of my fate,
      I am the captain of my soul.

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Re: Ask Quick Questions, Get Quick Answers [Re: CaptainTrips420]
    #29459712 -

Dang turns out 10 of my jars have this growth its very obvious its not mycelium when I broke and shook. I suspect it was the agar I got my vendor sold me garbage. that's the plates I got they said its a new formula to make it less sticky and they apologize for the clumps :shrug: they said they were certain it was contamination free.

Im just going to pour my own agar from now on :sadyes:

So should I toss the jars or is this normal?


--------------------
I am the master of my fate,
      I am the captain of my soul.

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Re: Ask Quick Questions, Get Quick Answers [Re: CaptainTrips420]
    #29459745 -

Not cobweb, but certainly mold


--------------------
"Remember, kids, the difference between science and screwing around is writing it down" -adam savage
Flowchart for Recommended plan of action.
Learn the tried and true way to grow mushrooms
Use the Damn search engine
After you know what you're doing, take a break 
Pick a book, Make some chips!
Josex said:Don't take the site seriously bro, ain't worth it.
 

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Re: Ask Quick Questions, Get Quick Answers [Re: tryptkaloids]
    #29459763 -

Shameful cross post from the TC thread (sorry) but would like to take action soon…

Hey team

Made a tub last night. 2 quarts in a 20qt pastey ez dial, about 20 cups or substrate, about 2.5 - 3 inches thick compressed

I went a bit heavy on the pseudo casing by accident …. It’s about 1 inch depth, all compressed

Should I remove some of the casing layer today or let it ride? If so, how do I go about doing so?

Thanks for the input


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Shop: North Spore Bulk Substrate   Myyco.com Golden Teacher Liquid Culture For Sale   Unfolding Nature Unfolding Nature: Being in the Implicate Order   Sporeworks.EU Spores for European Microscopy   Mushroom-Hut Substrate Bags   Original Sensible Seeds Bulk Cannabis Seeds   MagicBag.co All-In-One Bags That Don't Suck

Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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