Churpee Choozle said:

Or. Or. You wait two months and nothing happens.
I understand this to be a subtrop-specific problem.

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Did not know that, thanks. Only tried with dried cube grains, they come back real fast

squigglez said:

already posted these guys in cult pic of the day but was curious if these hats are a mutation or something else. these are gandalf from a trade.

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flapping_bird said:

those are pretty freaky! congrats! No idea what's happening, just wanted to recognize your grow - way to go!

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haha thx

cf7f36d6 said:

The most recent feedback I received was that I need to think about how dry my substrate is -- ie, that I was probably spraying too often. So my most recent batch, I probably went too far in that direction, and it dried out a bit. I then added some water directly to the side and let it soak in. Probably not great, but it seems to have revitalized. Some of the mycelium was a bit blue, but it doesn't seem to be mold.

Anyway, the few fruits that popped up pulled off the top layer of... something:




What's the deal here? I'm curious what this is, what it might indicate about my grow setup, etc.

FWIW, I'm using rye berry substrate and mixed into coir for bulk substrate. I think I did a better job of pasteurizing by heating the coir in a dutch oven at ~180 for a couple hours then letting cool before mixing in the inoculated spawn.

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callmehippie said:

Just put a hundred cryovials in the pc. When they came out every single one of them looks like they have a bunch of plastic looking shavings or flakes floating around inside now.







Has this ever happened to anybody? To be fair I did put them in on a grain run so they were in the PC for 2 hours.

But even still.  I'm not sure what the hell's going on here.

Anyways.  it's all sterile now. right? So  I should still be able to use them? Or do I need to start over?

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Phaethon said:

callmehippie said:

Just put a hundred cryovials in the pc. When they came out every single one of them looks like they have a bunch of plastic looking shavings or flakes floating around inside now.







Has this ever happened to anybody? To be fair I did put them in on a grain run so they were in the PC for 2 hours.

But even still.  I'm not sure what the hell's going on here.

Anyways.  it's all sterile now. right? So  I should still be able to use them? Or do I need to start over?

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Never seen that... Have you opened one to get a better feel for what that actually is? Did you use distilled water?

That's a lot of work to have to do again, but it would be even worse to lose all your cultures you thought you were backing up because whatever that is was harmful to the mycelium.

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AZ Starman2112 said:

Phaethon said:

callmehippie said:

Just put a hundred cryovials in the pc. When they came out every single one of them looks like they have a bunch of plastic looking shavings or flakes floating around inside now.







Has this ever happened to anybody? To be fair I did put them in on a grain run so they were in the PC for 2 hours.

But even still.  I'm not sure what the hell's going on here.

Anyways.  it's all sterile now. right? So  I should still be able to use them? Or do I need to start over?

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Never seen that... Have you opened one to get a better feel for what that actually is? Did you use distilled water?

That's a lot of work to have to do again, but it would be even worse to lose all your cultures you thought you were backing up because whatever that is was harmful to the mycelium.

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Hips…I’m going with the same advice here as Phaethon. I’d open one and feel what it is. It would suck to lose cultures to what you could have corrected. If it were me I’d just redo them anyway because it just don’t sit right with me. When I run cryos, I run them by themselves, not filled to the top, slightly loose lids, with the o-ring in place, and in the holder tray they come in, with the tray lid on but at an angle not closed.

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Splittinghairs said:

It’s normal from harvesting. The layer of mycelium pulls off from harvesting and just has coir left behind. Don’t overthink it.

As someone who has fucked up many grows from not properly doing field capacity, get that nailed down.

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Splittinghairs said:

And personally, you’re overthinking it by pasteurizing coir homie. Not worth the time or effort. Bucket, water and mix.

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callmehippie said:

AZ Starman2112 said:

Phaethon said:

callmehippie said:

Just put a hundred cryovials in the pc. When they came out every single one of them looks like they have a bunch of plastic looking shavings or flakes floating around inside now.







Has this ever happened to anybody? To be fair I did put them in on a grain run so they were in the PC for 2 hours.

But even still.  I'm not sure what the hell's going on here.

Anyways.  it's all sterile now. right? So  I should still be able to use them? Or do I need to start over?

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Never seen that... Have you opened one to get a better feel for what that actually is? Did you use distilled water?

That's a lot of work to have to do again, but it would be even worse to lose all your cultures you thought you were backing up because whatever that is was harmful to the mycelium.

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Hips…I’m going with the same advice here as Phaethon. I’d open one and feel what it is. It would suck to lose cultures to what you could have corrected. If it were me I’d just redo them anyway because it just don’t sit right with me. When I run cryos, I run them by themselves, not filled to the top, slightly loose lids, with the o-ring in place, and in the holder tray they come in, with the tray lid on but at an angle not closed.

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Thanks dudes.


So weird. AZ.  Everything you just explained  is exactly what I did except without putting the lid back on.

And it looks like if you were to take a knife and start to shave off some of the plastic of the actual tube or like pieces of the o ring. But the o rings appear ss to be unaffected as well. And the integrity of the tubes seems the same as well.

  I guess I'm going to open and check it out.

Then redo them.

I didn't clean them or anything before I loaded them up with regular tap water and then PC them.

But I didn't think that was necessary either. And even though the water was just plain tap water it was clean and debris free  when it went into the PC.

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cf7f36d6 said:

Splittinghairs said:

It’s normal from harvesting. The layer of mycelium pulls off from harvesting and just has coir left behind. Don’t overthink it.

As someone who has fucked up many grows from not properly doing field capacity, get that nailed down.

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I think I've got field capacity about right. The problem is that I started off by misting the surface a few times a day (because I had read somewhere that I should be doing that). And then I was dealing with some surface mold, etc., and I read that I shouldn't be spraying that often but keeping it much drier. Trying to get that right.

I get the impression that my yield is probably way less than it should be. I'm typically using about a quart of fully inoculated rye berries, then mixing it with coir at a ratio of 1 part rye to 3.5 parts coir by weight. Not a huge quantity of fruit coming off my bulk spawn, though. Is hydration (too much or too little) likely going to be a problem here?

Splittinghairs said:

And personally, you’re overthinking it by pasteurizing coir homie. Not worth the time or effort. Bucket, water and mix.

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Hah, thanks. As with similar hobbies (eg, homebrewing), I try to be very careful up-front.

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Well, what AZ said. I type too slow.
I only used distilled water for long term storage.

We have very hard tap water and those floaters can appear in boiled tap water here.

Please pardon me quoting me quoting myself from a thread that led to my LI tek.

Quote:

Q: Is using distilled water an absolute must? Is demineralized/deionized water not good enough?
A: tldr: Ideally, no. For short term use, probably. For long term storage, maybe.
Demineralized and deionized water both leave behind trace biological and particulate matter that distillation removes. Since we are going to sterilize the water any way, you might think that this is good enough. However, although the sterilization process will kill any nasties, it will not remove them or their byproducts. So you will not end up with true stasis until any live mycelium consumes or ignores any remnant materials or harmful byproducts.

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