B Traven said:

AZ Starman2112 said:

I have sent out a lot of wedges. All my wedges were from plates I cut into 8 pieces like a pizza pie. They were all transferred to tiny baggies inside de my SAB. They have all been pretty much clean and not only continued to grow myc in the baggies but some even pinned…or I guess got a bin! Like you guys have said it’s all in the methods and your work flow inside the SAB. And I’m not discounting a flow hood. I’d love to have one, but for now I can’t justify the cash outlay when my SAB hasn’t failed me yet.

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Right on.

So, you haven't found doing a bunch of wedges in the SAB to be too cumbersome, then?

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B, not at all. At first I got worried it would be a hassle, so I sacrificed a few agar plates and a handful of baggies to figure out how to do it and practice. Now I got a little system. The baggies come sealed and attached to each other at the ends where you open them. I put 8 bags in the SAB with the plate. I cut plate in 8. I do one slice at a time. I tear the bags in two, open a bag, hold it in one hand open and with that hand I open the plate and the other hand I slide the blade into the side of the agar slice just enough that I can handle the wedge and lift it to slide into the bag. I close lid, put down my scalpel and the lightly press the air out of the baggie and zip seal it. I reflame the blade and start the process over again.

Phaethon said:

I make 400ml of agar for 40 of the 60mm plates, so figure about 10ml/plate. I actually thought this was pretty thick, but this is clearly an area where we all have a different idea of how much is a heavy pour

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cronicr said:

Idk I'm guessing not long enough lol, prolly can't breathe there's no extra agar it's not ideal for sure

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B Traven said:

Sulfurshelfsean said:

B Traven said:

Ok, quick question.

And I really don't mean to be disrespectful in asking.

I still haven't found any good reason to use Liquid Cultures, Liquid Inoculant, or a Flowhood.

I can see how, in principle, these would all be useful if working at a particular scale or with particular types of vessels. But I haven't yet crossed a line where I thought, "man, I need these things." My time and effort bottlenecks are always on the other end- first with getting stuff spawned, then with getting stuff picked and processed.

So, can anyone tell me SPECIFICALLY why they use any of these, and what caused them to decide to switch?

Again, not trying to be disrespectful, but "I've always done it like this and it works" is not a satisfying answer to me.

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When i started out the guy whi taught me did MS to rye, then g2g. When I found out about taking clone tissue to lc and using inoculation ports to reduce contam risk it was a big step up fron what I was doing.
Opening less sterilized jars is a big positive when it comes to lc in my opinion.

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OK, right on.

So, was there no agar in that equation whatsoever?

It seems like I always just see people say that LC is faster, but I'm typically trying to slow things down a bit on that end of things. Staring at a mountain of too many plates and grain masters.

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JuniperTea said:

oh nooo side pins! (wuestion about what to do RIGHT NOW, not in the future, will do liners or pack better next time)

i am getting a nice healthy fruiting in my tubs, veils are breaking, im pulling them out as it happens.

however, i have a LOT of sidepins. what are some guidelines for harvesting them? they are still relatively small, no more than an inch or so long. I am afraid they will rot before the first flush is complete.

1) should i lift out the substrate and trim the sidepins off now, while the top is still actively fruiting?

2) should i wait until this flush is complete, then lift the substrate out, and trim them at that time? is there a risk of them starting to rot/ruin the tub? how do i know when the first flush is done (dumb question, i know).

i know its a lot of questions, and would probably be better for discord, but for some reason discord wont authenticate me, and i cant get into it.

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