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Re: Ask Quick Questions, Get Quick Answers [Re: ButtFace] * 1
    #29350910 -

ButtFace said:
BeefSupremeJr said:
Because the brick doesn't always weigh 650g. Its just what its labeled as. This is the reason I don't like coir "recipes".

I use a high-capacity scale and i weigh the brick. the math is as follows:

Brick weight (in grams) X 4.25 = Water volume (in ml)

It generally puts you on the dry side of field capacity but its real easy to add water and a real pita to remove it so its a good starting point.




I break up the bricks with a screwdriver and then weigh each buckets worth on a digital scale to 650 grams.



thats a bit of extra effort there. I just cook the brick whole. no fuss no muss.

Coco bliss tends to be a lot more consistent in terms of processing. Like tryp said, the cheaper shit tends to have a lot of fiber which is kindof useless.

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Re: Ask Quick Questions, Get Quick Answers [Re: BeefExtreme]
    #29350924 -

Hey fellas, would it be advantageous in any way to use PF Tek half pint WM jars with SHIP's as petri dishes for agar? My thoughts were, once I got a clean, strong specimen, I could just fill the jar with LC broth through the SHIP. It would be one less lid removal, but I don't know.


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Bees don't waste their time explaining to flies why honey is better than shit.

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Re: Ask Quick Questions, Get Quick Answers [Re: Darth Agar]
    #29350928 -

Lid removal is safer than a ship. But you can absolutely grow a clean culture out on agar, and add lc broth. Or you can make the agar extra strong and just add sterile water.

See - Pastywhyte's EzLC tek.


--------------------
"Remember, kids, the difference between science and screwing around is writing it down" -adam savage
Flowchart for Recommended plan of action.
Learn the tried and true way to grow mushrooms
Use the Damn search engine
After you know what you're doing, take a break 
Pick a book, Make some chips!
Josex said:Don't take the site seriously bro, ain't worth it.
 

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Re: Ask Quick Questions, Get Quick Answers [Re: tryptkaloids]
    #29350933 -

Thanks pardner, I imagine a half pint of LC would noc 4 or 5 quarts of grain?


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Bees don't waste their time explaining to flies why honey is better than shit.

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Re: Ask Quick Questions, Get Quick Answers [Re: Darth Agar]
    #29350942 -

more like ten quarts if you ration it out. Just depends on your expectations for colonization time.

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Re: Ask Quick Questions, Get Quick Answers [Re: BeefExtreme] * 1
    #29350946 -

:thumbup: Thanks Beef.


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Bees don't waste their time explaining to flies why honey is better than shit.

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Re: Ask Quick Questions, Get Quick Answers [Re: Darth Agar]
    #29350951 -

You bet. by the way, I do agree with my colleague tryp on the ships. Just don't, bud. Keep us updated! holler if you need anything else.

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Re: Ask Quick Questions, Get Quick Answers [Re: Darth Agar]
    #29350958 -

Dealing with some kind of Bacteria in my plates, all of my plates, that is showing up as an orange color in the agar when you flip them.

16 Varieties (14 cubes, 2 pan cyan) and seems like pretty much everything has it.

Have tried sequestering multiple times, sandwiching, lots of transfers.  Dialed my agar to 25g agar/15g ME per L.  Have gone through a ton of plates.  I'll get nice clean rhizo growth on the sequestered plates, xfer them and then see the orange appear eventually on the transfer piece initially and end up on the new growth/plates over time.

Headscratcher where it came from, might have come from an outdoor print that came from India I had, when I was laying a ton of stuff onto agar from prints.  Very confounding though that every single plate has it, given that genetics came from a variety of sources (4-5)

My technique seems air tight (99%).  Very old spores laid to plates that never restarted, those plates are 100% clean (15+), no bacteria or contamination of any kind on any of them, zero growth.  So I'm ruling out any contam from the Plates themselves, the agar itself along with autoclaving that correctly (20 mins) and pouring is just fine.  Seems like only the transfers getting the contaminated agar/myc gets it.

Attempted sequestered plates that never succeeded in bridging the sequester gap, those plates are also 100% w/o contamination.  While the plates where the myc bridged the sequester gap to the other side seem to keep it.

This leads me to believe that the bacteria is stuck in the myco somehow, there's a word for this, maybe someone can remind me.

I'm using a 48" FFU for a hood its 6 months old, fresh MERV14 pre-filter, and a standalone HEPA with a UV-C in my lab room that runs 247365.  Induction coil, afaik tools are clean and not the issue (no signs of anything in the agar where I cool the blade on any plates). Have been using a drenching excess of 71% ISO for any clean work, like overkill.


Thoughts, suggestions, ideas?
I'm seeing improvements w/ each sequestered transfer but just can't seem to shake it entirely.














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Re: Ask Quick Questions, Get Quick Answers [Re: Wiscoregon] * 1
    #29350969 -

I'm not seeing bacteria boss. You mean the yellowing? That just kinda happens with age, especially with the exotics. these look like good plates to me doot i would send em.

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Re: Ask Quick Questions, Get Quick Answers [Re: BeefExtreme]
    #29350992 -

I'm not entirely convinced tbh.  The orange / yellow is definitely something growing, I know that's the case because all the previous plates that have it eventually get wrecked by it and eventually I get metabolites on those plates

But before my most recent transfers (the nice rhizo plates) I did a josex poke LC with 6 of the best looking of them to a LC gel with a little agar to thicken it up for pouring into jars.  Those went to Oats 2 days ago, so that will give me confirmation soon, But I'm about 95% sure they're all gonna be bac.


Here's a few examples of the older plates you can see it gets more severe with age.














Side note, I have already taken some of these to grain just to try to get some fruits, they were quite bacterial (smell etc), but gave me a first flush, two cubes varieties.  One bacterial pan went to a shoebox, but stalled out.  I dumped those three shoeboxes outside under a bag and a half of black kow, the pan has flushed once outside (currently second), the two cubes haven't (its been over 90F here daytime, so probably need to cool off 10F before they pop?)  I got so frustrated trying to clean them, I started to think just leaving them outside, taking prints and then starting fresh is my best option.

I ate the first flush of pans (only 8g) wet friday with another 4g dried cubes.  First time eating Pans, quite enjoyed them.  I see why people like them so much.





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Re: Ask Quick Questions, Get Quick Answers [Re: Wiscoregon]
    #29351007 -

Those plates do look fucked compared to the last ones, also that bulk sub look incredibly saturated. That cant be helping.


--------------------
"Remember, kids, the difference between science and screwing around is writing it down" -adam savage
Flowchart for Recommended plan of action.
Learn the tried and true way to grow mushrooms
Use the Damn search engine
After you know what you're doing, take a break 
Pick a book, Make some chips!
Josex said:Don't take the site seriously bro, ain't worth it.
 

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Re: Ask Quick Questions, Get Quick Answers [Re: tryptkaloids]
    #29351009 -

(RE Saturation) You talking about the outdoor patch?

That's just straight up BK out of the bag I've been hitting with water every few days, piled on some old shoebox cakes, Covered in some cardboard to keep it out of the sun and damp.  It's been hot / dry here so kinda surprised they're fruiting at all.

Edited by Wiscoregon (09/21/25 04:40 PM)

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Re: Ask Quick Questions, Get Quick Answers [Re: Wiscoregon]
    #29351014 -

Couple more things on my mind while I'm here:

Best way to preserve pans is low heat dry?  Assuming the heat / o2 destroys the psilocin but that's my best available option if I want to preserve them?

Pans are new to me, do they hate being misted directly? Does this stunt pins / fruits?  I know they prefer a marta with 97% ish and regular fae and I know some have succeeded in tubs using coir sub enriched with cactus fert.

Any suggestions or guidelines for this outdoor patch sustaining fruiting conditions?

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Re: Ask Quick Questions, Get Quick Answers [Re: Wiscoregon]
    #29351017 -

Hellooooo !

I know we don't see a lot, but could it be early myc ? Just to know if i'm growing some obvious contam or no :grin:
It has shown after 4 days, and it's barely expanding since 2 days but still do.

The dark spot is some spores.

Ty !



--------------------
wow

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Re: Ask Quick Questions, Get Quick Answers [Re: Tropical]
    #29351028 -

Looks like a germinated spore to me with some possible contam nearby (white patches not near spore)

4 days to germinate is pretty normal. Ime 2 days with almost no growth after germination is a bit odd, and I’m also used to seeing more white in the patch and maybe some fluff 2 days after germ.

Keep monitoring them, I expect they’ll grow and you can sector a transfer in a few days

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Re: Ask Quick Questions, Get Quick Answers [Re: philmyco]
    #29351070 -

So im attempting ro cross golden teacher with jack frost from spore swabs heres what my gar looks like after swabbing both caps i circled spots where i believe i seen 2 diff types of kycwlium growth forming .. do i transfer these to other plates label and grow out for results ?


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Re: Ask Quick Questions, Get Quick Answers [Re: funguy883]
    #29351110 -

funguy883 said:
So im attempting ro cross golden teacher with jack frost from spore swabs heres what my gar looks like after swabbing both caps i circled spots where i believe i seen 2 diff types of kycwlium growth forming .. do i transfer these to other plates label and grow out for results ?




Ye


--------------------
A Love Letter to New Growers
The 7 Edicts of Mushroom Cultivation as Decreed by Smellyhobbit

:kodama:


Anyone can get a canopy. But can you get what you want?

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Re: Ask Quick Questions, Get Quick Answers [Re: tryptkaloids] * 1
    #29351214 -

tryptkaloids said:
Lid removal is safer than a ship. But you can absolutely grow a clean culture out on agar, and add lc broth. Or you can make the agar extra strong and just add sterile water.

See - Pastywhyte's EzLC tek.




If you plan to use a ship. Do like faht.

Sterilizing SHIP’s after an ISO wipe


--------------------
:hippie::mushroom2:Take it easy. if it's easy. take it twice:mushroom2::mushroom2:
:sporedrop:The Hitchhiker's Guide to the Shroomery:sporedrop:

**A LINK TO EVERTHING**


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Re: Ask Quick Questions, Get Quick Answers [Re: callmehippie] * 2
    #29351243 -





I was about to spawn two jars of x7x+ and two of blue meanie, was getting help from my wife this time And I think we mixed up the jars. Pretty sure one of each went into the tub. I was assuming the x7s were the white ones and BM were the brown ones? Or is it not that easy to tell?

If the x7s were the ones I thought they were they seem a lot different than the B+ I have been growing. I can’t wait to try them. Might even make some micros with them this week.

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Re: Ask Quick Questions, Get Quick Answers [Re: ButtFace] * 1
    #29351273 -

ButtFace said:
I have a question about the Bucket TEK. Why is that, when using the same recipe of 1 gallon of water to 650 grams of coir, the coir sometimes comes out to field capacity when other times it comes out soupy and verm must be added to it to get it to field capacity? Same inputs and same brand of coir.



1 gallon of water is 890g of dry coir, that could be why

890x4.25=3,782.5 ml
1 gallon of water is 3785 ml

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Shop: Mushroom-Hut Substrate Bags   Sporeworks.EU Spores for European Microscopy   Original Sensible Seeds Bulk Cannabis Seeds   MagicBag.co All-In-One Bags That Don't Suck   Myyco.com Golden Teacher Liquid Culture For Sale   North Spore Injection Grain Bag


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