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InvisiblefahtsterM
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Sterilizing SHIP’s after an ISO wipe * 21
    #27586183 - 12/18/21 01:00 PM (2 years, 1 month ago)



DISCLAIMER: I understand that the CDC states that water must be pc’d to kill endospores but those endospores need to be present to cause problems.  City tap water hasn’t been a problem for me using this method.  It’s the same reason we can boil tap water and suck it up a few times to produce viable syringes… it works over and over again.  BUT if you do feel the need to pc your water to make it sterile by CDC standards, it’s an easy fix… just run your pc (you’ll probably want to include your hosing in that pc run as well) at 15psi for a half hour and let the pressure drop back out to 0, remove the hose and attach it to the port and bring back up to a boil and revent for 10 minutes.  There ya go, lab sterile water.  Again, I’m not seeing these endospores so until I do, I’ll be skipping the pc water run.  I will say that the pressure is at about 5psi when I run steam pen so that’s about 230F if that makes you feel any better

Yoyoyo party peeps!

So the thread title has been a problem I’ve been trying to solve for a loooong time.. I use SHIP’s because I refuse to open a jar after its been sterilized (unless it’s in a sterile environment like a pc’d oven bag) until it’s fully colonized.  SHIPs work great but their downfall is that you have to poke a needle through them which means there’s contact and anytime there’s contact, that’s another contamination vector.  ISO sanitizes but not all bacteria are killed by ISO so eventually you’re going to get a dirty poke… at least a poke that makes your jar unusable.  I’d say I’m at 1 in 30 qts is unusable, atm.  There’s a lot more times where the poke introduces a small amount of bacteria and the jar colonizes fine and puts out a decent enough flush where it’s barely noticeable that the jar had any bacteria..

But that’s still a problem because I use glc jars for inoculating my qts.. it works great.. whenever I make qts, I just make another glc jar that I knock up when I inoculate the qts to keep the process going but those slight bacterial infections build up (unless I dry the glc grains out but that takes weeks to be able to use the culture again) and by the 5th (or sooner) round of glc grains -> qts -> glc grains, the culture is bacterial to the point that it’s unusable because of all the contact of the needles on SHIPs

This is the downfall of SHIPs and why you won’t see a lot of top growers using them. So how do you fix that?  I’ve thought of iodine, I’ve tried H2o2 .. you can stick the hot needle through the port but then the silicone won’t self heal and the lid is shot after one use.. that’s no good either.

Eventually I came to the conclusion that the best way to sterilize a SHIP is with the same thing we use to sterilize our grain.. steam.  Periodically I’d do a google search for small fabric steamers but they’re too big and I didn’t want to steam the whole jar lid.. I wanted it to be a very centralized and controlled

Recently I was leaning on my counter in my kitchen waiting for my pc to vent and watching steam shoot out of the rocker weight port on the pc and it hit me… if I could get a hose small enough to fit around that port, that would be perfect!

Luckily I own an airizer extreme Q vaporizer and have a replacement hose that’s meant for high heat… it’s the tiniest bit too big for the port so I have to wrap a 2” sliver of painters tape around the very top of the port but works great to make a seal and the steam pen was born.

I wrap and tape some paper towel around the tubing near the tip so that I can hold it because it will get HOT.

It’s probably not necessary but I pour some H2o2 through the tubing just to rinse it out and sanitize it a bit before using

I fill the pc with a decent amount of water.. since the pc will be basically venting the whole time, more water should be used than what you’d use if you were running grain jars.. depending on how many jars I have to inoculate, I’d say I put in at least 4-6 qts of water… but if you run the pc dry, just add more water, obviously.

Put the lid on and attach the hose to the rocker weight port.  The other end of the hose (with the paper towel wrapped “handle”) I place in a 10” pot to catch any water condensation that comes out of the hose


Turn the stove on high and once steam starts coming out the end of the tubing, I start a timer for 15 minutes… this vents the pc so that all unsterilized air is out of the pc and only steam is coming out and it sterilizes the inside of the hose… before anyone asks, I check the temp at the end of the tubing before I proceed.. once it’s @ 200F, I call it good


I like to keep the pot close to the stove vent so I can exhaust the excess steam

After 15 minutes, we’re ready to inoculate jars.  I place a folded up piece of paper towel (or a hand towel) under one side of the bottom of the jar that’s opposite the SHIP so that the jar tilts slightly.. this will make it so any pooling condensed water runs off the SHIP and down the side of the jar (this further cleans the SHIP as any particles that didn’t get sterilized are blown off the SHIP surface by the jet of steam)


So I prepare the SHIPs on my jars like I normally would.. wiping the shit out of them with an iso pad.. after I’ve done that, I leave the iso pad sitting on the SHIP covering it while I torch the needle on my lc syringe

once it’s red hot, I set it down next to the jar and wipe the SHIP on my jar one last time… grab the steam pen (SP) from the pot and hold it vertically over the injection site on the SHIP for 4 seconds to sterilize that spot

Then I move the SP off to the side of the injection site so that it’s shooting the steam jet over the injection site and off the edge of the SHIP and jar
(I use large SHIPs so I can rest the end of the SP directly on the SHIP surface).

This is all done very quickly so your needle should still be hot

Pick up the syringe and move the needle into the steam jet and push out a few drops of liquid to “cool” the needle enough so that it doesn’t melt a hole into SHIP.

At this point, I puncture the needle into the SHIP and slowly push it through the SHIP into the jar.. all while in the jet of steam.  I do it slowly so the shaft of the needle can be further sterilized by the steam jet

Once the needle is in as far as you want it to be, remove the SP and place back in the pot, inject the liquid and pull the needle out

I realize the actual injection procedure might be hard to visualize just from words and it’s hard to take pics by myself and there’s steam involved so I made a diagram


That’s it!  So simple… it’s like having a very tiny flow hood at the injection site but instead of using filtered air, it’s using sterilized air/steam.  I want to instill just how important and exciting this is for me… it’s the cheat code, the bees knees, the 1up mushroom, finding $5,000 in a winter jacket… the end of dirty jars… I can do regular LC’s using SHIPs… I can reuse glc jars that grow back and reharvest the myc.  Once I have a clean culture contained, that’s it, it remains clean.  This is huge

So far I’ve done this with 12 qts and a few 4oz jars.. here’s a few.  Qts a couple days after shake


4oz glc extension jar


But I’m so confident in the process that I’m not even worried that’s it’s not going to work.. it’s too simple and there’s nothing standing in the way of it working.  Those ^^^ jars are colonizing beautifully with no condensation in the jars even with huge temp swings and the logic is solid (any little bit of bacteria that remains after an iso wipe is either sterilized and/or blown off the SHIP by the steam and if the needle is “cooled” in the jet of steam, there’s nowhere for the contamination to enter the equation)

The only thing I’m pissed about is that I didn’t think of doing it sooner.  And I’m not looking to take credit for anything.. if this something that already exists, that’s cool.. I’m just calling it a steam pen because that’s how I hold it and it’s shorter than steam gun sterilizer lol. Just sharing something that’s gonna change my cult life in the hopes that it helps others.  :thumbup:

Faht


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InvisibleMunchauzen
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Re: Sterilizing SHIP’s after an ISO wipe [Re: fahtster] * 3
    #27586188 - 12/18/21 01:04 PM (2 years, 1 month ago)

you made a fucking steam sterilizing gun, you god damn mad mycologist.

:soproud:


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OfflineLand TroutM
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Re: Sterilizing SHIP’s after an ISO wipe [Re: Munchauzen]
    #27586218 - 12/18/21 01:30 PM (2 years, 1 month ago)

:rockon:


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OfflineJoeFell
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Re: Sterilizing SHIP’s after an ISO wipe [Re: Land Trout]
    #27586287 - 12/18/21 02:31 PM (2 years, 1 month ago)

This is beyond over kill.  Using a flowhood will do what your attempting 1000 times more effective.  If your worried about keeping the self healing injection port sterile the trick is simple dude. Just get a put a cotton ball on top of the ship and soak it in alcohol so each time you push your red hot syringe tip through its being pushed through liquid alcohol.  This plus a flowhood. 


What you have almost built is a steam pasteurizer.  Just need a bin or a big tub to pump that steam into and you can pasteurize large amounts of substrate and casing.


Edited by JoeFell (12/18/21 02:32 PM)


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OfflineMycoplex
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Re: Sterilizing SHIP’s after an ISO wipe [Re: JoeFell]
    #27586296 - 12/18/21 02:39 PM (2 years, 1 month ago)

Thanks for this writeup faht!


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InvisibleMunchauzen
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Re: Sterilizing SHIP’s after an ISO wipe [Re: JoeFell] * 1
    #27586300 - 12/18/21 02:43 PM (2 years, 1 month ago)

Quote:

JoeFell said:
This is beyond over kill.  Using a flowhood will do what your attempting 1000 times more effective.  If your worried about keeping the self healing injection port sterile the trick is simple dude. Just get a put a cotton ball on top of the ship and soak it in alcohol so each time you push your red hot syringe tip through its being pushed through liquid alcohol.  This plus a flowhood. 


What you have almost built is a steam pasteurizer.  Just need a bin or a big tub to pump that steam into and you can pasteurize large amounts of substrate and casing.



Yikes. Alcohol sanitizes it does not sterilize. What you suggested would not keep anything sterile, as alcohol is not sterile itself. What he DID build is a steam sterilizer, its by no means a pasteurizer. Pastuerizing occurs at temps around 60-70C, water isn't converted to steam until 100C. Steam sterilizes.


Edited by Munchauzen (12/18/21 04:31 PM)


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OfflineForresterM
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Re: Sterilizing SHIP’s after an ISO wipe [Re: JoeFell]
    #27586301 - 12/18/21 02:47 PM (2 years, 1 month ago)

Quote:

JoeFell said:
Just get a put a cotton ball on top of the ship and soak it in alcohol so each time you push your red hot syringe tip through its being pushed through liquid alcohol.





If you read the post he explained exactly why this doesn't work in this situation.


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OfflineCamera93
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Re: Sterilizing SHIP’s after an ISO wipe [Re: Forrester]
    #27586314 - 12/18/21 03:02 PM (2 years, 1 month ago)

Great write up Faht! Thanks for sharing with the community :hatsoff:


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Close your eyes, and do the best that you can



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InvisibleLemgrub
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Re: Sterilizing SHIP’s after an ISO wipe [Re: JoeFell]
    #27586319 - 12/18/21 03:06 PM (2 years, 1 month ago)

Quote:

JoeFell said:
This is beyond over kill.  Using a flowhood will do what your attempting 1000 times more effective.  If your worried about keeping the self healing injection port sterile the trick is simple dude. Just get a put a cotton ball on top of the ship and soak it in alcohol so each time you push your red hot syringe tip through its being pushed through liquid alcohol.  This plus a flowhood. 


What you have almost built is a steam pasteurizer.  Just need a bin or a big tub to pump that steam into and you can pasteurize large amounts of substrate and casing.




:foreheadslap:

Faht this is awesome. You're like one of these guys that shows up from reddit talking about thinking outside the box, except you actually know what the hell you're doing:rockon:


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InvisiblefahtsterM
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Re: Sterilizing SHIP’s after an ISO wipe [Re: Lemgrub]
    #27586844 - 12/19/21 01:34 AM (2 years, 1 month ago)

Quote:

JoeFell said:
This is beyond over kill.  Using a flowhood will do what your attempting 1000 times more effective.  If your worried about keeping the self healing injection port sterile the trick is simple dude. Just get a put a cotton ball on top of the ship and soak it in alcohol so each time you push your red hot syringe tip through its being pushed through liquid alcohol.  This plus a flowhood. 


What you have almost built is a steam pasteurizer.  Just need a bin or a big tub to pump that steam into and you can pasteurize large amounts of substrate and casing.




Yeah.. it seems like you didn’t read the op lol.

Thanks for the props y’all

Faht


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Invisiblesandman420
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Re: Sterilizing SHIP’s after an ISO wipe [Re: fahtster] * 1
    #27586937 - 12/19/21 06:28 AM (2 years, 1 month ago)

That's wildly clever idea for those using ports. Always :popcorn: seeing the clever ways you get it done.

With the trademark hand drawn accoutrement (please pronounce extra French) and everything. Nicely done.


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Re: Sterilizing SHIP’s after an ISO wipe [Re: sandman420]
    #27588048 - 12/20/21 08:14 AM (2 years, 1 month ago)

Great thinking Faht!


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OfflineAbuSharka
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Re: Sterilizing SHIP’s after an ISO wipe [Re: Defacto2]
    #27588170 - 12/20/21 10:57 AM (2 years, 1 month ago)

:rockon:  :rockon:


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InvisibleBaba Yaga
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Re: Sterilizing SHIP’s after an ISO wipe [Re: AbuSharka]
    #27588246 - 12/20/21 12:19 PM (2 years, 1 month ago)

Yo faht, doing the good work as always.

I bought a big box of syringes but stopped using them because of this problem, now I use syringes just for the josex poke.

But I'm still thinking about this and have another idea.

How about putting 3 or 4 layers of tape over the port and every time you going to stab the port you can wipe the lid with ISO and peel off
one layer leaving you with a sterile surface. Tape needs to be non adsorbent and survive the PC cycle of course.

Like this:



I established a non syringes workflow now and will probably never get around to try this.


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OfflineMegaCrouton
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Re: Sterilizing SHIP’s after an ISO wipe [Re: JoeFell] * 1
    #27588249 - 12/20/21 12:22 PM (2 years, 1 month ago)

Quote:

JoeFell said:
This is beyond over kill.  Using a flowhood will do what your attempting 1000 times more effective.  If your worried about keeping the self healing injection port sterile the trick is simple dude. Just get a put a cotton ball on top of the ship and soak it in alcohol so each time you push your red hot syringe tip through its being pushed through liquid alcohol.  This plus a flowhood. 


What you have almost built is a steam pasteurizer.  Just need a bin or a big tub to pump that steam into and you can pasteurize large amounts of substrate and casing.




BeSides the reasons listed in the main post, I'd be hesitant to stick a red hot syringe tip through soaked cotton. It's going to give you a flaming cotton ball


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Invisiblemicelio
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Re: Sterilizing SHIP’s after an ISO wipe [Re: MegaCrouton]
    #27588381 - 12/20/21 02:14 PM (2 years, 1 month ago)

Hey Faht...!

Your an interesting person to follow...

Thanks for the steam sterilization idea...  :thumbup:


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InvisiblefahtsterM
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Re: Sterilizing SHIP’s after an ISO wipe [Re: Baba Yaga]
    #27588440 - 12/20/21 03:03 PM (2 years, 1 month ago)

Quote:

Baba Yaga said:
Yo faht, doing the good work as always.

I bought a big box of syringes but stopped using them because of this problem, now I use syringes just for the josex poke.

But I'm still thinking about this and have another idea.

How about putting 3 or 4 layers of tape over the port and every time you going to stab the port you can wipe the lid with ISO and peel off
one layer leaving you with a sterile surface. Tape needs to be non adsorbent and survive the PC cycle of course.

Like this:



I established a non syringes workflow now and will probably never get around to try this.




I like the idea.. I actually had a similar idea a while ago where I was going to just stick tape on the port as soon as it came out of the pc until I was ready to use it just to keep dust and shit off it.. there’s a problem though… tape doesn’t stick to silicone easily if at all. 

Besides the vent up time, it’s actually faster using this than before when I wasn’t using it.. now I’m not suuuuper worried making sure the port is as clean as gods ass.. I still clean it really really well but now I’m like, that’s good enough.. the SP will get er done.  I know I wrote out a lot with diagrams and shit lol but the whole process is like 15 seconds per inoculation, including torching the needle

Thanks for the love peeps!


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InvisibleBaba Yaga
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Re: Sterilizing SHIP’s after an ISO wipe [Re: fahtster]
    #27588459 - 12/20/21 03:21 PM (2 years, 1 month ago)

True, I didn't think of the silicone being non stick. Could work with the prefab rubber ports maybe.

Sounds like you got a good system going there  :wink::thumbup:


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InvisibleSmartattack
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Re: Sterilizing SHIP’s after an ISO wipe [Re: Baba Yaga] * 3
    #27588553 - 12/20/21 04:30 PM (2 years, 1 month ago)

I love Faht's hyper elaborate way of doing things many take for granted by doing normal stuff. Its like the shroomiest way of doing mushroom stuff ever. I honestly dont even understand but bow in respect lol.

This is why spores from Faht are extra special to me, I know the time and care that go into them and they always give up the goods. Im thinking of making a hardwood humidor to store them in between uses.


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InvisibleDrboomer
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Re: Sterilizing SHIP’s after an ISO wipe [Re: Smartattack] * 2
    #27588598 - 12/20/21 05:31 PM (2 years, 1 month ago)

The crazy part is at first it seems out there. Like a mad scientist. Then you try to follow it, it makes sense. Then you try it and you understand perfectly.

It's all about making no hood work for you.


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