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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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Re: Ask Quick Questions, Get Quick Answers [Re: Proposition 122]
    #29332680 -

Thank you for your input and for responding to my post. I remember reading early on the importance of venting, and I think you did a nice job explaining how it works. Thanks.

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Re: Ask Quick Questions, Get Quick Answers [Re: callmehippie]
    #29332681 -

callmehippie said:
look for ice on the heat sinks? in the unit behind the filters?

Or on The outside of the unit on And directly under the filter?


Or am I needing to try to look under the housing and pay attention to the pump itself if it's even visible?


The side that blows cold air is called the evaporator. That radiator lookin thing (evaporator) can ice over. The compressor ir "pump" should be in the bottom of the unit. It will get holy shit hot if the evap ices up. If you are running it full blast, non-stop, there is a chance it could freeze. If you have it set to shut off periodically via a thermostat, you should be good. Watch for water pooling under it. Thats your 1st indication of an issue.


--------------------



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Re: Ask Quick Questions, Get Quick Answers [Re: humble student]
    #29332685 -

humble student said:
PC Sterilization Issue Maybe?
I autoclaved plates that had been pre-poured with agar, vented PC 10 minutes, and maintained PC at 17 psi for 20 minutes. Plates were moved to the SAB and wrapped. After several days, just about every plate was contaminated, and I’m wondering if I did not PC long enough to achieve sterilization.
I’m using 75x15 and 100x15 glass plates. Also, I loaded the PC with a lot, including two 500ml media bottles about 3/4 full of agar.
Finally, the question: How long is it recommended to PC pre-poured plates? And, is a longer time necessary when the PC is more full vs less full?


if your two media bottles havnt contamed, ur vent/cook times are fine. wont hurt anything to bump them up a little if ur tweaking about it tho

are these vented plates? that seems like a crazy amount of mold. i pour plates in front of a ffu and let them cool in open air with the machine off and never get anything like that. maybe a random nasty here and there. i do set my empty media bottle on top of the stack to keep the lids snuggly down but idk if that even makes a difference

either way youre better off in the long run just pouring them in ur sab

good luck!

Edited by sloug (09/02/25 02:00 AM)

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Re: Ask Quick Questions, Get Quick Answers [Re: The Tao]
    #29332686 -

The Tao said:
H
Proposition 122 said:
The Tao said:
How do you have a closed environment if the PC is venting?


once removed from heat the PC stops venting (jiggle stops) almost immediately.


Venting means that air is able to move in or out of a space. How would a PC come back down to atmospheric pressure overnight if it was a closed environment?


The Tao said:
H
Proposition 122 said:
The Tao said:
How do you have a closed environment if the PC is venting?


once removed from heat the PC stops venting (jiggle stops) almost immediately.


Venting means that air is able to move in or out of a space. How would a PC come back down to atmospheric pressure overnight if it was a closed environment?


If you remove the jiggle weight and release pressure before the natural cooling reaches zero it will suck in ambient non sterile air.
The PC comes back down to atmospheric pressure because temps drop. Cooling steam condenses back into water. Condensing steam -> reduced gas volume -> internal pressure is lowered and returns to atmospheric.
I'm not saying the jiggle weight is airtight, only a barrier at that stage. They are designed NOT to be airtight.


--------------------
"Let yourself be silently drawn by the strange pull of what you really love. It will not lead you astray." – Divan-e-Shams

How to upload Photos

The Hitchhiker's Guide to the Shroomery

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Golden Teachers

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Re: Ask Quick Questions, Get Quick Answers [Re: Proposition 122]
    #29332688 -

whatchu say bout my momma?!?

it cools while simultaneously pulling in air thru the jiggler thingy. its not the cooling itself that returns the cooker to atmospheric pressure. unlike an aa steam sterilizer with a control valve that completely seals. i always let it cool to the touch before opening it and it is still holding a vacuum. i then proceed to open the valve and suck in all that nasty room air

        :sunny:
:boombox2:

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Re: Ask Quick Questions, Get Quick Answers [Re: sloug]
    #29332691 -

sloug said:
<snip> whatchu say bout my momma?!?
it cools while simultaneously pulling in air thru the jiggler thingy.

No. Remove from heat. Pressure inside the PC is positive (albeit dropping). As long as your PC is hot to the touch the internal pressure is above atmospheric. The bad guys cannot get in. Pressure only moves out, not in.

You momma speaks highly of me :happiness:


--------------------
"Let yourself be silently drawn by the strange pull of what you really love. It will not lead you astray." – Divan-e-Shams

How to upload Photos

The Hitchhiker's Guide to the Shroomery

Fruiting Cubes


Golden Teachers

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Re: Ask Quick Questions, Get Quick Answers [Re: Proposition 122] * 3
    #29332696 -

humble student said:
I agree, Starman, I was amazed at all the different appearing stuff growing on the plates. And, I like your comment about the plates looking like they came out of a CDC lab; maybe more like a lab in Wuhan.

i know you were making a crappy joke and probably didnt even catch it, but this is a mildly xenophobic comment. Labs all over the world carry cultures of many many molds bacteria and viruses. Many of which are dangerous human pathogens. The idea that a lab in wuhan is dirtier than any other lab is a short sighted one at best.


--------------------
"Remember, kids, the difference between science and screwing around is writing it down" -adam savage
Flowchart for Recommended plan of action.
Learn the tried and true way to grow mushrooms
Use the Damn search engine
After you know what you're doing, take a break 
Pick a book, Make some chips!
Josex said:Don't take the site seriously bro, ain't worth it.
 

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Re: Ask Quick Questions, Get Quick Answers [Re: Proposition 122]
    #29332709 -

Proposition 122 said:
sloug said:
<snip> whatchu say bout my momma?!?
it cools while simultaneously pulling in air thru the jiggler thingy.

No. Remove from heat. Pressure inside the PC is positive (albeit dropping). As long as your PC is hot to the touch the internal pressure is above atmospheric. The bad guys cannot get in. Pressure only moves out, not in.

You momma speaks highly of me :happiness:


something has to replace that pressure being released during cool down or else a vacuum would form. if we were sterilizing with compressed air i would agree with you

...sorta

:cookiemonster:

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Re: Ask Quick Questions, Get Quick Answers [Re: sloug]
    #29332713 -

How much wheat should I put in my jars?

Are they going to expand even more after the pc?

Looking at the markings on the sides of the jars. if they go up to 800 what are you guys filling to?


This is what I got. Should I not go any further than this? Or can I go a little further?



--------------------
:hippie::mushroom2:Take it easy. if it's easy. take it twice:mushroom2::mushroom2:
:sporedrop:The Hitchhiker's Guide to the Shroomery:sporedrop:

**A LINK TO EVERTHING**


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Re: Ask Quick Questions, Get Quick Answers [Re: sloug]
    #29332716 -

sloug said:
something has to replace that pressure being released during cool down or else a vacuum would form. if we were sterilizing with compressed air i would agree with you

...sorta

:cookiemonster:


Nothing is being replaced while the pressure drops. That said, it will come back to atmospheric pressure long before it's cooled to room temperature.

Gas and most liquids will expand and shrink depending on temperature. So after reaching atmospheric pressure the PC, jars and sterile condition inside the PC starts to drop in temperature. The gases and liquid will start to shrink, because cooling removes kinetic energy from the gas molecules, causing them to slow down and move less, resulting in decreased volume and pressure.

That is when there will be unclean air getting inside your PC and it is no longer a sterile environment inside :uhoh:

callmehippie said:
How much wheat should I put in my jars?

Are they going to expand even more after the pc?

Looking at the markings on the sides of the jars. if they go up to 800 what are you guys filling to?


This is what I got. Should I not go any further than this? Or can I go a little further?




Wheat will expand a little in the jar, other then that I think it's a matter of preference. I started out filling to the 800ml mark, now I do 600ml for cubes and 500ml for exotics, mostly because it fits my needs for the tub and tray sizes I use, but it also makes break&shake easier and I like the thought of a little more room for GE.


--------------------
“All Fungi are edible.

Some fungi are only edible once.”
― Terry Pratchett


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Re: Ask Quick Questions, Get Quick Answers [Re: Kloakk] * 1
    #29332720 -

nothing is being replaced ONLY IF the pc is completely sealed air tight. the presto jigglers dont create a good seal. u might not hear air entering back in but, it is. you have to take into account the venting cycle which removes air from the chamber before closing the valve or presto weight and bringing up to temp.

regardless, the "unclean" air entering back in isnt even an issue

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Re: Ask Quick Questions, Get Quick Answers [Re: sloug] * 1
    #29332721 -

sloug said:
nothing is being replaced ONLY IF the pc is completely sealed air tight. the presto jigglers dont create a good seal. u might not hear air entering back in but, it is. you have to take into account the venting cycle which removes air from the chamber before closing the valve or presto weight and bringing up to temp.

regardless, the "unclean" air entering back in isnt even an issue





You have an incorrect understanding of basic physics


--------------------
Hitchhikers Guide to the Shroomery





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Re: Ask Quick Questions, Get Quick Answers [Re: callmehippie]
    #29332724 -

callmehippie said:
How much wheat should I put in my jars?

Are they going to expand even more after the pc?

Looking at the markings on the sides of the jars. if they go up to 800 what are you guys filling to?


This is what I got. Should I not go any further than this? Or can I go a little further?





I fill to 600 with rye or wheat. As long as you have room to shake and break you'll be fine.


--------------------
Hitchhikers Guide to the Shroomery





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Re: Ask Quick Questions, Get Quick Answers [Re: Kloakk]
    #29332726 -

Would it be all right to get some no-poures prepped tonight and then sterilize them tomorrow?

like prep the agar and pour them, let them set, wrap them.  and PC tomorrow morning?


--------------------
:hippie::mushroom2:Take it easy. if it's easy. take it twice:mushroom2::mushroom2:
:sporedrop:The Hitchhiker's Guide to the Shroomery:sporedrop:

**A LINK TO EVERTHING**


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Re: Ask Quick Questions, Get Quick Answers [Re: callmehippie]
    #29332735 -

Yes


--------------------
"Remember, kids, the difference between science and screwing around is writing it down" -adam savage
Flowchart for Recommended plan of action.
Learn the tried and true way to grow mushrooms
Use the Damn search engine
After you know what you're doing, take a break 
Pick a book, Make some chips!
Josex said:Don't take the site seriously bro, ain't worth it.
 

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Re: Ask Quick Questions, Get Quick Answers [Re: tryptkaloids]
    #29332736 -

Helps with condensation too


--------------------
"Remember, kids, the difference between science and screwing around is writing it down" -adam savage
Flowchart for Recommended plan of action.
Learn the tried and true way to grow mushrooms
Use the Damn search engine
After you know what you're doing, take a break 
Pick a book, Make some chips!
Josex said:Don't take the site seriously bro, ain't worth it.
 

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Re: Ask Quick Questions, Get Quick Answers [Re: tryptkaloids]
    #29332738 -

tryptkaloids said:
Helps with condensation too


It actually doesn't because you will just get condensation after pc anyway.

The trick is to allow them to dry out after.

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Re: Ask Quick Questions, Get Quick Answers [Re: DERRAYLD]
    #29332739 -

Groovy.  Thanks.


--------------------
:hippie::mushroom2:Take it easy. if it's easy. take it twice:mushroom2::mushroom2:
:sporedrop:The Hitchhiker's Guide to the Shroomery:sporedrop:

**A LINK TO EVERTHING**


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Re: Ask Quick Questions, Get Quick Answers [Re: sloug] * 1
    #29332740 -

sloug said:
nothing is being replaced ONLY IF the pc is completely sealed air tight. the presto jigglers dont create a good seal. u might not hear air entering back in but, it is. you have to take into account the venting cycle which removes air from the chamber before closing the valve or presto weight and bringing up to temp.

regardless, the "unclean" air entering back in isnt even an issue


Well, if I'm reading your post right, we are saying the same thing.

While the pressure drop nothing is entering the PC. After pressure is equalized temperature continues to drop and then unclean air is getting in the PC, is that what you're saying?


--------------------
“All Fungi are edible.

Some fungi are only edible once.”
― Terry Pratchett


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Re: Ask Quick Questions, Get Quick Answers [Re: DERRAYLD] * 1
    #29332748 -

DERRAYLD said:
tryptkaloids said:
Helps with condensation too


It actually doesn't because you will just get condensation after pc anyway.

The trick is to allow them to dry out after.


I suppose i assumed he was gonna lid and foil em in the morning


--------------------
"Remember, kids, the difference between science and screwing around is writing it down" -adam savage
Flowchart for Recommended plan of action.
Learn the tried and true way to grow mushrooms
Use the Damn search engine
After you know what you're doing, take a break 
Pick a book, Make some chips!
Josex said:Don't take the site seriously bro, ain't worth it.
 

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