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Re: Ask Quick Questions, Get Quick Answers [Re: Dynasty]
    #29332587 -

That's a brilliant idea, Dynasty! Both 500mL media bottles containing agar that were PCed with the plates show no signs of contamination!

I believe Tryptkaloids and LadysKnight are correct; the contamination must be occurring when moving the plates from the PC to the SAB, and/or in my SAB procedures.

Thanks for your input.

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Re: Ask Quick Questions, Get Quick Answers [Re: BeefExtreme] * 1
    #29332589 -

BeefSupremeJr said:
tryptkaloids said:
Quote:
BeefSupremeJr said:l

man, you could split the hair of a frog


Sure. The main point im trying to drive is hay has seeds.

Also, wheat grass is just young wheat.

Okay im done. I just like clearly defined terms.


okay but youre doing the opposite if im understandind right.  youre trying to say two totally different things are the same and they are not.  hay and straw have a lot more that sets them apart than just "seeds".  comparing apples to artichokes here.  i dont remember anyone arguing that hay didnt have seeds but i still dont see what point youre trying to arrive at.  sometimes i feel like if i say something is blue, youll show up just to say its red because its me who said it.

ill admit that i might be both misunderstanding what youre saying and that we could possibly be agreeing on a thing, and also maybe im reading into things a bit much and youre just this argumentative with everyone. 


My point is entirely how its used. I was trying to add details that might be pertinent. Apologies for making you feel like im just trying to argue with you specifically. Was never my intention. I often get told im argumentative, i guess its just my hyperliteral brain.  My point is i wouldnt ever try to pasteurize hay, it need sterilized. Straw can be pasteurized no problem.

Similar in the garden, i would expect hay mulch to germinate all over the place where straw is better for weed supression.

Maybe its my rural redneck background but ive always called threshed wheat straw and unthreshed, intact, baled wheat - hay.

Maybe ive always simplified it that way in my head because mushrooms are my only use case.

Either way i wasnt trying to pick a fight, im just not the best communicator and can be confidently wrong too


--------------------
"Remember, kids, the difference between science and screwing around is writing it down" -adam savage
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Re: Ask Quick Questions, Get Quick Answers [Re: Proposition 122]
    #29332590 -

I'll vent 20 minutes next time, thanks for the suggestion.

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Re: Ask Quick Questions, Get Quick Answers [Re: Proposition 122]
    #29332595 -

Proposition 122, I'm not challenging your statement or disagreeing with you. Please help me understand how removing the pressure regulator will compromise the sterility of what's inside the PC.

And, Thanks for responding to my post!

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Re: Ask Quick Questions, Get Quick Answers [Re: tryptkaloids] * 1
    #29332599 -

tryptkaloids said:
BeefSupremeJr said:
tryptkaloids said:
Quote:
BeefSupremeJr said:l

man, you could split the hair of a frog


Sure. The main point im trying to drive is hay has seeds.

Also, wheat grass is just young wheat.

Okay im done. I just like clearly defined terms.


okay but youre doing the opposite if im understandind right.  youre trying to say two totally different things are the same and they are not.  hay and straw have a lot more that sets them apart than just "seeds".  comparing apples to artichokes here.  i dont remember anyone arguing that hay didnt have seeds but i still dont see what point youre trying to arrive at.  sometimes i feel like if i say something is blue, youll show up just to say its red because its me who said it.

ill admit that i might be both misunderstanding what youre saying and that we could possibly be agreeing on a thing, and also maybe im reading into things a bit much and youre just this argumentative with everyone. 


My point is entirely how its used. I was trying to add details that might be pertinent. Apologies for making you feel like im just trying to argue with you specifically. Was never my intention. I often get told im argumentative, i guess its just my hyperliteral brain.  My point is i wouldnt ever try to pasteurize hay, it need sterilized. Straw can be pasteurized no problem.

Similar in the garden, i would expect hay mulch to germinate all over the place where straw is better for weed supression.

Maybe its my rural redneck background but ive always called threshed wheat straw and unthreshed, intact, baled wheat - hay.

Maybe ive always simplified it that way in my head because mushrooms are my only use case.

Either way i wasnt trying to pick a fight, im just not the best communicator and can be confidently wrong too


ok.  my bad.  i farmed most of my life. i was one of the last guys i knew who still ran an old square baler for the neighbors horses.

baled pasture grass and thats what we call hay.  still green when you bale it. weighs a ton. high in protein and very nutritious. & yes wherever you leave it sit around it will make a mess of grass and weeds sprouting.  i wouldnt even attempt to sterilize it personally but pasteurizing is out of the question. just way too nutritious.  everything wants a bite.

straw is just  like you say.  the waste product of wheat production.  hollow stocks.  you can leave it sit for years. never molds unless you leave it wet.  perfect substrate.  so light i can throw a bail over a fence with one hand.

seems like we are saying the same thing.  sorry bud.


--------------------
The Cultivation Confessional Booth - The OFFICIAL Cultivation Off Topic Discussion

✸-✸-✸

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Edited by BeefExtreme (09/01/25 10:39 PM)

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Re: Ask Quick Questions, Get Quick Answers [Re: humble student] * 2
    #29332618 -

humble student said:
Proposition 122, I'm not challenging your statement or disagreeing with you. Please help me understand how removing the pressure regulator will compromise the sterility of what's inside the PC.

And, Thanks for responding to my post!


Better to let the unit cool down radiantly with as few avenues as possible for filtration/infiltration to occur. This happens to everything in the pc as well, so it's best to let it all sit.

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Re: Ask Quick Questions, Get Quick Answers [Re: humble student] * 1
    #29332628 -

humble student said:
I'll vent 20 minutes next time, thanks for the suggestion.


This is always a good idea, and if you have to bump up your actual agar cook time go for it. 30 minutes won't hurt anything. I PC my grains but I use an Instantpot for my agar and I do it for an hour.

I use glass plates and they can get dirty real quick just from the air. They don't really seal and all kinds of bad stuff can easily get in. I love them, but you really have to be careful.


--------------------
Hitchhikers Guide to the Shroomery





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Re: Ask Quick Questions, Get Quick Answers [Re: humble student]
    #29332630 -

humble student said:
That's a brilliant idea, Dynasty! Both 500mL media bottles containing agar that were PCed with the plates show no signs of contamination!

I believe Tryptkaloids and LadysKnight are correct; the contamination must be occurring when moving the plates from the PC to the SAB, and/or in my SAB procedures.

Thanks for your input.


I’ve personally never pre pour plates so not sure where the problem is there, but pretty confident that’s where the contam came from.

I always pc the agar and pour once it’s cooled enough to handle.


--------------------
Beefs Hitchhikers guide
D3s agar tek

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Re: Ask Quick Questions, Get Quick Answers [Re: Dynasty]
    #29332638 -

Not a cult question. 

But you guys seem pretty handy.

Just wanted to check in. My AC went out and I've got this portable AC unit in my room.

The filters that keep the dust out is basically just a window screen. I'm constantly having to clean it.

I decided today I was going to cut up one of my fiberglass AC filters and tape it to the outside. Like a pre-filter.









My question is,  is this okay?
Should this still allow an ample amount of air flow in order to allow this thing to function at its best?

I'm asking because I know my central AC unit in my house is a lot more powerful.

so I'm just double checking.


--------------------
:hippie::mushroom2:Take it easy. if it's easy. take it twice:mushroom2::mushroom2:
:sporedrop:The Hitchhiker's Guide to the Shroomery:sporedrop:

**A LINK TO EVERTHING**


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Re: Ask Quick Questions, Get Quick Answers [Re: the_chosen_one]
    #29332643 -

PC-ing creates a closed sterile environment. Once you open the lid, or on a smaller scale open the vent, the bad guys have access to your goods. Leave the jiggle weight in place until you open the lid to remove your goods in front of the flow hood.

Also, there is a tek here somewhere on the importance of venting and why he recommends 20mins over 10mins. Improper venting can leave pockets of DRY heat. You want to achieve all STEAM heat.


--------------------
"Let yourself be silently drawn by the strange pull of what you really love. It will not lead you astray." – Divan-e-Shams

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Re: Ask Quick Questions, Get Quick Answers [Re: Proposition 122] * 2
    #29332653 -

How do you have a closed environment if the PC is venting?

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Re: Ask Quick Questions, Get Quick Answers [Re: callmehippie]
    #29332657 -

callmehippie said:
Not a cult question. 

But you guys seem pretty handy.

Just wanted to check in. My AC went out and I've got this portable AC unit in my room.

The filters that keep the dust out is basically just a window screen. I'm constantly having to clean it.

I decided today I was going to cut up one of my fiberglass AC filters and tape it to the outside. Like a pre-filter.









My question is,  is this okay?
Should this still allow an ample amount of air flow in order to allow this thing to function at its best?

I'm asking because I know my central AC unit in my house is a lot more powerful.

so I'm just double checking.


It should be. Even with a higher merv it should flow plenty. Just keep an eye on condensation and icing.


--------------------
You can ignore the truth to your final breath. But trust me, it will meet you there to be ignored no longer.

The answer to your question is almost always 42
“The answer is always grow more shroom.” Mr Piggy
- Those two answers coexist peacefully.

Quick Sterilization Reference for Altitude

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Re: Ask Quick Questions, Get Quick Answers [Re: Proposition 122] * 1
    #29332658 -

Agar, as a liquid medium, doesn't really have quite the same issues with dry air pockets. If steam and pressure from the main vessel fail to penetrate, and there's a pocket of dry air under the lid of a plate, then the already-liquid agar will just come to a boil and fill the space with steam.


I use a SAB for transfers and an instant pot for agar. Stacks of plates wrapped in foil, go from the IP (long after everything has cooled down) and straight to the rack in the SAB. Unwrapped inside the SAB riggt before transfers are carried out.

If I get any contamination, I'm confident that it's happening the RIGHT way: either massive blunders in sterile technique when making transfers, or just incredibly dirty cultures that I'm too busy to properly clean up and too attached to ditch.


--------------------


ACAB  🖕🧊


Edited by B Traven (09/02/25 08:31 AM)

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Re: Ask Quick Questions, Get Quick Answers [Re: The Tao]
    #29332659 -

The Tao said:
How do you have a closed environment if the PC is venting?


once removed from heat the PC stops venting (jiggle stops) almost immediately.


--------------------
"Let yourself be silently drawn by the strange pull of what you really love. It will not lead you astray." – Divan-e-Shams

How to upload Photos

The Hitchhiker's Guide to the Shroomery

Fruiting Cubes


Golden Teachers

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Re: Ask Quick Questions, Get Quick Answers [Re: humble student]
    #29332661 -

humble student said:
I'll vent 20 minutes next time, thanks for the suggestion.


Moisture transfers heat better than air. Steam sterilization at 121°C (250°F) for 20 minutes is considered a standard for achieving sterility. Dry heat is much less efficient at transferring thermal energy (air is a poor conductor compared to steam), you need both a higher temperature and a longer time to achieve the same microbial kill. So, dry heat at 250°F (121°C) is not sufficient to match steam sterilization at the same temperature, it would take much longer.


--------------------
"Let yourself be silently drawn by the strange pull of what you really love. It will not lead you astray." – Divan-e-Shams

How to upload Photos

The Hitchhiker's Guide to the Shroomery

Fruiting Cubes


Golden Teachers

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Re: Ask Quick Questions, Get Quick Answers [Re: B Traven]
    #29332662 -

I've always vented for 10 minutes like the manual for the Presto says to do. And never had any issues. Most of the teks and write-ups you see around here suggests 10 to 15 minutes. I've never seen anybody suggest 20. But its not hurting anything if you do.

Here's this write up with the title you were talking about but he also is only saying to vent for 10.

The Importance of Venting a Pressure Cooker or Autoclave




Not sure this is what you were referring to though since he's not saying anything about 20 minutes.

Well maby once here. Sorta.



--------------------
:hippie::mushroom2:Take it easy. if it's easy. take it twice:mushroom2::mushroom2:
:sporedrop:The Hitchhiker's Guide to the Shroomery:sporedrop:

**A LINK TO EVERTHING**


Edited by callmehippie (09/01/25 11:43 PM)

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Re: Ask Quick Questions, Get Quick Answers [Re: 1NFIN8]
    #29332665 -

1NFIN8 said:
callmehippie said:
Not a cult question. 

But you guys seem pretty handy.

Just wanted to check in. My AC went out and I've got this portable AC unit in my room.

The filters that keep the dust out is basically just a window screen. I'm constantly having to clean it.

I decided today I was going to cut up one of my fiberglass AC filters and tape it to the outside. Like a pre-filter.









My question is,  is this okay?
Should this still allow an ample amount of air flow in order to allow this thing to function at its best?

I'm asking because I know my central AC unit in my house is a lot more powerful.

so I'm just double checking.


It should be. Even with a higher merv it should flow plenty. Just keep an eye on condensation and icing.


:whathesaid:

If you choke the airflow below a certain point on an AC unit of any size, it will ice over and burn up the "pump". Keep an eye on it.


--------------------



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Re: Ask Quick Questions, Get Quick Answers [Re: Gilmour Hendrix]
    #29332669 -

look for ice on the heat sinks? in the unit behind the filters?

Or on The outside of the unit on And directly under the filter?


Or am I needing to try to look under the housing and pay attention to the pump itself if it's even visible?


--------------------
:hippie::mushroom2:Take it easy. if it's easy. take it twice:mushroom2::mushroom2:
:sporedrop:The Hitchhiker's Guide to the Shroomery:sporedrop:

**A LINK TO EVERTHING**


Edited by callmehippie (09/02/25 12:01 AM)

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Re: Ask Quick Questions, Get Quick Answers [Re: Proposition 122] * 1
    #29332670 -

H
Proposition 122 said:
The Tao said:
How do you have a closed environment if the PC is venting?


once removed from heat the PC stops venting (jiggle stops) almost immediately.


Venting means that air is able to move in or out of a space. How would a PC come back down to atmospheric pressure overnight if it was a closed environment?

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Re: Ask Quick Questions, Get Quick Answers [Re: AZ Starman2112] * 1
    #29332674 -

I agree, Starman, I was amazed at all the different appearing stuff growing on the plates. And, I like your comment about the plates looking like they came out of a CDC lab; maybe more like a lab in Wuhan.

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