BeefSupremeJr said:

phytophthora said:

I think part of the confusion is we sometimes muddle the ideas of monocultures and monokaryons. A diykaryotic culture is a mated pair of spores, and has the ability to produce sexual organs. A monokaryotic culture is a single spore growing out and searching for a mate, and cannot reproduce sexually yet. Further complicating things is that a monokaryon can mate with a (by definition already mated) dikaryon, and that you can physically separate dikaryons back into non-sexual monokaryons, that can then go on to form new dikaryons or as stated previously mono-dikaryotic mated pairings.

A monoculture to my knowledge would be two single confirmed monokaryons that have mated to form a single dikaryon. Often what happens is thousands of dikaryons (and mono/di pairings) coming together on our plates.


Edited to add - sorry if I am preaching to the choir here!

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no no thank you i really appreciate this. thanks for clearing that up for me. so what of the monoculture spores? how much genetic differentiation is within?

so,

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I think a monokaryon is like an egg or a sperm. Takes two to tango, so I suppose each is represented equally. Except instead of a sperm fertilizing an egg, the monokaryons fuse and contain two nuclei in each cell. So instead of sex it’s like literally melding together and becoming one single organism, that is now sexually capable, and can “breed” with itself, in effect creating mushrooms that will release spores. But that sexual pairing can also include a third individual. Mushroom sex is fucking bizarre… and I like it a lot.

BeefSupremeJr said:

did you use two piece lids? Rather than throwing them away, I would be tempted to sterilize some lids wrapped in tinfoil and then switch them in the sab..

or

Sterilize some polyfil in tinfoil.  Poke a hole in the lid (after sanitizing it with iso) with a red hot ice pick, tape the polyfil over the hole with micropore tape (in the sab)

i guess id try either of those things before dumping.  cant break whats broke.

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tryptkaloids said:

Ill read the chatgpt nonsense later, but i thought phytopthora did an amazing job of explaining that. Ive tried to wrap my head around the topic for years and i think the last few pages helped

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POWAtrippin said:

Basidia doesnt form as a single genetic unit the until right before spore production

No longer haploid cells but with two separate sets of genetic in one cell until the fruiting body is produced.  When the actual genetic combination happens is right before spore production.

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phytophthora said:

Well damn Got some reading to do myself apparently.

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??? I thought chat validated what you said and just added to the explanation. I certainly didn't mean to offend or contradict you. I was just trying to understand this better and shared what I found. My apologies. I appreciate your posts

phytophthora said:

Interesting insight there. Catnip knows his shit and can explain all this better than I ever could. The floor is yours…

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I agree catnip knows his shit, but reading him talk about it in the past has led me to confusion. He may understand it better, and i love being proven wrong, but idk if he can explain it better lol he knows too much, idk if he can dumb it down for me

phytophthora said:

Also just to be pedantic, monokaryons can exist for a very long time in their “vegetative” state as an individual until a mate is found. So there is lonely, non-sexually viable mycelium out there on the pull. Hungry, and ready to fuse. 

Edit to add - thank you!

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callmehippie said:

Got blood test results today for the ivf.

confirmed.  She's 🤰 bitchessssss!!!





No need for everybody to congratulate us again. Lol.

Im Just over the moon right now.
all levels and and labs were also off the charts. They said things couldn't be better at this point. Really hoping it works out this time. 🤞🏼


Update shared.

MUSH LOVE

☆CMH☆

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Hey. I didn't get a chance to congratulate you since I missed it completely.
CONGRATS