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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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Re: Ask Quick Questions, Get Quick Answers [Re: Lemgrub]
    #29328050 -

Lemgrub said:
Proposition 122 said:
Lemgrub said:
Proposition 122 said:
Does each spore have the same, or different, genetics?


Different. More or less same as sperm in humans.


So if I am thrilled with my harvest, and want more :grin: I wouldn't go back to the spore print I would go to ___________.


Clone your favorite fruit, I use criteria like the first to mature, one from a nice cluster, or largest. Then you've locked in that genetic set for further propagation. You can still get it from going back to the original spore print but might take some time.


So if I had a prized mushroom, I took a piece of it (clone), and propagated that I would have identical genetics? So that piece would not be sterile, so I would be back to agar again and start over? What if I had made a LC from the same myc that I fruited? Identical genetics?


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Edited by Proposition 122 (08/28/25 01:49 PM)

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Re: Ask Quick Questions, Get Quick Answers [Re: Lemgrub]
    #29328051 -

Hey mush fam!

I was wondering does Cronicrs container tek pf one works with steamer also?

My mycelium didnt colonized good in the boxes and i too early put to fruiting conditions, what do you suggest to put lid over again and wait for colonizing or just wait in fruit conditions?

Many thanks

Edited by ogelite (08/28/25 01:54 PM)

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Re: Ask Quick Questions, Get Quick Answers [Re: BeefExtreme]
    #29328054 -

BeefSupremeJr said:
However, two things will never ever happen:

1.  theyll never have 2 identical kids


You sure about this?


--------------------
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Re: Ask Quick Questions, Get Quick Answers [Re: BeefExtreme] * 2
    #29328056 -

I think part of the confusion is we sometimes muddle the ideas of monocultures and monokaryons. A diykaryotic culture is a mated pair of spores, and has the ability to produce sexual organs. A monokaryotic culture is a single spore growing out and searching for a mate, and cannot reproduce sexually yet. Further complicating things is that a monokaryon can mate with a (by definition already mated) dikaryon, and that you can physically separate dikaryons back into non-sexual monokaryons, that can then go on to form new dikaryons or as stated previously mono-dikaryotic mated pairings.

A monoculture to my knowledge would be two single confirmed monokaryons that have mated to form a single dikaryon. Often what happens is thousands of dikaryons (and mono/di pairings) coming together on our plates.
:raveface:

Edited to add - sorry if I am preaching to the choir here!

Edited by phytophthora (08/28/25 02:08 PM)

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Re: Ask Quick Questions, Get Quick Answers [Re: Proposition 122] * 1
    #29328062 -

Taking a tissue sample to agar is called cloning in our circles, but realistically is often a sampling of many different cultures cooperating. It’s not like taking a plant cutting, which is an exact dna match to the parent plant. See my post above about mono and dikaryotic pairings.

Edited to add - if you want the exact same performance, I would go back to the LC you used originally, it should contain all the same genetic info even if it represents many individual genetic lines.

Edited by phytophthora (08/28/25 02:07 PM)

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Re: Ask Quick Questions, Get Quick Answers [Re: phytophthora] * 1
    #29328066 -

Ok. Time for me to dive into learning all of this stuff. I came in looking for a medicinal solution to TRD. Now the hobby and science has me hooked.


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Re: Ask Quick Questions, Get Quick Answers [Re: tryptkaloids]
    #29328088 -

tryptkaloids said:
BeefSupremeJr said:
However, two things will never ever happen:

1.  theyll never have 2 identical kids


You sure about this?


positive

if youre reffering to "identical twins" you might wanna look into that.


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The Cultivation Confessional Booth - The OFFICIAL Cultivation Off Topic Discussion

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Edited by BeefExtreme (08/28/25 03:16 PM)

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Re: Ask Quick Questions, Get Quick Answers [Re: phytophthora]
    #29328090 -

phytophthora said:
I think part of the confusion is we sometimes muddle the ideas of monocultures and monokaryons. A diykaryotic culture is a mated pair of spores, and has the ability to produce sexual organs. A monokaryotic culture is a single spore growing out and searching for a mate, and cannot reproduce sexually yet. Further complicating things is that a monokaryon can mate with a (by definition already mated) dikaryon, and that you can physically separate dikaryons back into non-sexual monokaryons, that can then go on to form new dikaryons or as stated previously mono-dikaryotic mated pairings.

A monoculture to my knowledge would be two single confirmed monokaryons that have mated to form a single dikaryon. Often what happens is thousands of dikaryons (and mono/di pairings) coming together on our plates.
:raveface:

Edited to add - sorry if I am preaching to the choir here!


no no thank you i really appreciate this. thanks for clearing that up for me. so what of the monoculture spores? how much genetic differentiation is within?

so,


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The Cultivation Confessional Booth - The OFFICIAL Cultivation Off Topic Discussion

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Re: Ask Quick Questions, Get Quick Answers [Re: BeefExtreme] * 7
    #29328103 -



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:mushroom2: LotKid's PE Tips        :shpongle:    LotKid's Chocolates :mushroom2:
:dancingbear: :dancingbear:

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Re: Ask Quick Questions, Get Quick Answers [Re: LotKid] * 1
    #29328112 -


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Re: Ask Quick Questions, Get Quick Answers [Re: Arkyhog]
    #29328122 -



--------------------

:mushroom2: LotKid's PE Tips        :shpongle:    LotKid's Chocolates :mushroom2:
:dancingbear: :dancingbear:

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Re: Ask Quick Questions, Get Quick Answers *DELETED* [Re: milkboy]
    #29328129 -

Post deleted by 8760

Reason for deletion: Right to Erasure

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Re: Ask Quick Questions, Get Quick Answers [Re: 8760] * 3
    #29328130 -

yup. go ahead and sterilize the whole jar with grains in too if you're really going for it :lol:


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Learn it, know it, live it


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Re: Ask Quick Questions, Get Quick Answers [Re: BeefExtreme]
    #29328132 -

BeefSupremeJr said:
tryptkaloids said:
BeefSupremeJr said:
However, two things will never ever happen:

1.  theyll never have 2 identical kids


You sure about this?


positive

if youre reffering to "identical twins" you might wanna look into that.


You can't have identical children... But you can have the same child twice... At the same time.

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Re: Ask Quick Questions, Get Quick Answers [Re: dr_drums]
    #29328137 -

dr_drums said:
BeefSupremeJr said:
tryptkaloids said:
BeefSupremeJr said:
However, two things will never ever happen:

1.  theyll never have 2 identical kids


You sure about this?


positive

if youre reffering to "identical twins" you might wanna look into that.


You can't have identical children... But you can have the same child twice... At the same time.



what?


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The Cultivation Confessional Booth - The OFFICIAL Cultivation Off Topic Discussion

✸-✸-✸

The Hitchhiker's Guide to the Shroomery

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Re: Ask Quick Questions, Get Quick Answers [Re: 8760]
    #29328139 -

8760 said:
milkboy said:
BeefSupremeJr said:
Milkboy used to run unmodded lids all the time.  i personally dont love the idea but it does work.


Used to? Still do fam :headbanger:



What's the technique for unmodified lids? I just sterilize the 2-piece lids and flip the cap upside down, and leave the threads a little loose?


The way I saw milky do it he just used the gray plastic lids and basically just have them tightened just to where the threads begin to catch


--------------------
The Cultivation Confessional Booth - The OFFICIAL Cultivation Off Topic Discussion

✸-✸-✸

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Re: Ask Quick Questions, Get Quick Answers *DELETED* [Re: Mackload]
    #29328140 -

Post deleted by 8760

Reason for deletion: .

Edited by 8760 (08/28/25 04:54 PM)

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Re: Ask Quick Questions, Get Quick Answers *DELETED* *DELETED* [Re: BeefExtreme]
    #29328141 -

Post deleted by 8760

Reason for deletion: .

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Re: Ask Quick Questions, Get Quick Answers [Re: LotKid] * 1
    #29328142 -

LotKid said:
B Traven said:
LotKid said:
Okay... the answer is... the angle of elevation of the sun at the time of local observation divided by the square root of strawberry rhubarb pie....


:solved:


I don't know if the question has been asked yet but there's the answer. Hope it helps. :bongload:


Thanks, that really is helpful information, but I'm afraid I'm still seeing a lot of side-fumbling.


Did you make sure to secure the gerter spring to the rear part of the propeller shaft by turning the tungsten locking collar counter clockwise until it clicks into place? 

Sometimes it feels like it clicked in but if it's not fully secured then it will effect the gimble inverter.


Ah, no, you're right, I didn't.

My tungsten locking collar keeps rubbing against the squigules on the propeller shaft. Do you think lubing it with gypsum would help?


--------------------


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Re: Ask Quick Questions, Get Quick Answers [Re: 8760]
    #29328143 -

did you use two piece lids? Rather than throwing them away, I would be tempted to sterilize some lids wrapped in tinfoil and then switch them in the sab..

or

Sterilize some polyfil in tinfoil.  Poke a hole in the lid (after sanitizing it with iso) with a red hot ice pick, tape the polyfil over the hole with micropore tape (in the sab)

i guess id try either of those things before dumping.  cant break whats broke.


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The Cultivation Confessional Booth - The OFFICIAL Cultivation Off Topic Discussion

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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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