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Re: Ask Quick Questions, Get Quick Answers [Re: ThurmanMurman]
    #29256857 -

ThurmanMurman said:
I had some grains sitting around in sterile packaging for a while before putting them to agar on 6/4 and I'm just now seeing them come to life. Is that normal?



Was the sterile packaging paper backed or was there a way for the grains to continue drying out?
Only ask because I stored a heeeeaaap of cultures on grains in sterile autoclave pouches and none of them revived unless they were less than a month or 2 old, I can only assume there’s a limit to how much you can dry them out till they’re dead, I now just pop them in ziplocks to limit access to air and they’ve been fine up to a year so far.

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Re: Ask Quick Questions, Get Quick Answers [Re: 3.A.M] * 1
    #29256858 -

3.A.M said:
ThurmanMurman said:
I had some grains sitting around in sterile packaging for a while before putting them to agar on 6/4 and I'm just now seeing them come to life. Is that normal?



Was the sterile packaging paper backed or was there a way for the grains to continue drying out?
Only ask because I stored a heeeeaaap of cultures on grains in sterile autoclave pouches and none of them revived unless they were less than a month or 2 old, I can only assume there’s a limit to how much you can dry them out till they’re dead, I now just pop them in ziplocks to limit access to air and they’ve been fine up to a year so far.



I've pulled dried grains out of grain jars that sat for over a year and was able to revive them on agar and brf pucks. They were modified jar lids so I'm sure they just dessicated over time from sitting.

Edited by Axman (06/20/25 07:27 PM)

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Re: Ask Quick Questions, Get Quick Answers [Re: Dkeppel] * 2
    #29256860 -

Dkeppel said:
I've made some Acidulated extraction for mushroom gummies and drops, I know this stuff is usually for gummies/drops and the chocolate tek's I see are made with dry mush but I thought maybe I could reduce this stuff down into a powder to add to chocolate or something?



Adding water based liquid to melted chocolate will cause it to seize. I would use mush powder for chocolate, or the tea for gummies.

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Re: Ask Quick Questions, Get Quick Answers [Re: RoscoeReturns]
    #29256864 -

First time grower here. I wanted to grow nats in 2 tubs.
What is the best room (in a small apartment) and setup to place the tubs in when they are fruiting?

Should I place a small grow tent inside the shower?
Above my fridge?
On the table in the kitchen close to the window for more fresh air?

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Re: Ask Quick Questions, Get Quick Answers [Re: Illegal_Phoenix]
    #29256865 -

Illegal_Phoenix said:
Illegal_Phoenix said:
Is this Contamed?



bump



How many yesses do you need?


--------------------
🅃🄴🄰🄼 🄸🄷🄾🄿


New to cultivation or the shroomery?  Observe the hitchhiker's guide

The Hitchhiker's Guide to the Shroomery



Smellyhobbit said:
"Wiping a needle with isopropyl after flame sterilization is like shitting after you wipe your ass."

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Re: Ask Quick Questions, Get Quick Answers [Re: Damayo]
    #29256867 -

Damayo said:
First time grower here. I wanted to grow nats in 2 tubs.
What is the best room (in a small apartment) and setup to place the tubs in when they are fruiting?

Should I place a small grow tent inside the shower?
Above my fridge?
On the table in the kitchen close to the window for more fresh air?



Hello and welcome to the Shroomery.

Read the Hitchhiker's Guide in my signature and you'll get all of the updated and trusted guides that we use.

The best place for your tubs is anywhere you feel they won't be disturbed a lot. I've grown on the counter, in a closet, in the basement. All of these places work and the inside of the tub will have its own relative humidity so the humidity of the room that it is stored in does not matter.

The average 6qt shoebox tote at any big box store will have adequate fresh air exchange without modification or fanning.

I wish you the best.

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Re: Ask Quick Questions, Get Quick Answers [Re: tryptkaloids] * 1
    #29256876 -

I thought the liquid might be an issue.

I'd like to use fresh mush in chocolate somehow, maybe reducing off all the liquid will work :shrug:

I'll experiment and report back if I figure it out.

Thanks guys :rockon:

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Re: Ask Quick Questions, Get Quick Answers [Re: Axman] * 2
    #29256900 -

Axman said:
Damayo said:
First time grower here. I wanted to grow nats in 2 tubs.
What is the best room (in a small apartment) and setup to place the tubs in when they are fruiting?

Should I place a small grow tent inside the shower?
Above my fridge?
On the table in the kitchen close to the window for more fresh air?



Hello and welcome to the Shroomery.

Read the Hitchhiker's Guide in my signature and you'll get all of the updated and trusted guides that we use.

The best place for your tubs is anywhere you feel they won't be disturbed a lot. I've grown on the counter, in a closet, in the basement. All of these places work and the inside of the tub will have its own relative humidity so the humidity of the room that it is stored in does not matter.

The average 6qt shoebox tote at any big box store will have adequate fresh air exchange without modification or fanning.

I wish you the best.




A closet might get a bit stale... some where that had indirect sunlight with in direct cross breeze that's easily maintained around 65-76°f (shoot for 73° so a little deviation either way is still perfect) ... and away from pets/kids/pigs

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Re: Ask Quick Questions, Get Quick Answers [Re: LewDoja] * 1
    #29256904 -

:cantarguewiththat:


--------------------
🅃🄴🄰🄼 🄸🄷🄾🄿


New to cultivation or the shroomery?  Observe the hitchhiker's guide

The Hitchhiker's Guide to the Shroomery



Smellyhobbit said:
"Wiping a needle with isopropyl after flame sterilization is like shitting after you wipe your ass."

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Re: Ask Quick Questions, Get Quick Answers [Re: Dkeppel]
    #29256963 -

Dkeppel said:
I thought the liquid might be an issue.

I'd like to use fresh mush in chocolate somehow, maybe reducing off all the liquid will work :shrug:

I'll experiment and report back if I figure it out.

Thanks guys :rockon:



I bet if fully dehydrated it would work.  I’ve got some instant tea crystals stored in the freezer right now.  Let us know how it goes, I may give it a try as well. :rockon:

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Re: Ask Quick Questions, Get Quick Answers [Re: Axman] * 1
    #29256968 -

Axman said:
Has anyone used a Instapot with the Sous Vide mode to prep grain?

I've been trying to nail down a temperature and time with it, but when I tried to copy Trip's formula for a real Sous Vide I didn't end up with properly hydrated grains.



Same.  I’ve seen 150f for 90 min documented a few times for rye.  I tried that with millet but I’m not sure that it was quite enough for the hard seed. Still experimenting...

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Re: Ask Quick Questions, Get Quick Answers [Re: nocap] * 1
    #29256971 -

nocap said:
Axman said:
Has anyone used a Instapot with the Sous Vide mode to prep grain?

I've been trying to nail down a temperature and time with it, but when I tried to copy Trip's formula for a real Sous Vide I didn't end up with properly hydrated grains.



Same.  I’ve seen 150f for 90 min documented a few times for rye.  I tried that with millet but I’m not sure that it was quite enough for the hard seed. Still experimenting...



Yeah I did 150f for 90min and it wasn't right I kept upping the time by 15-30m and I overshot it once and undershot it as well :shrug:

I just wasn't sure if anyone else had experience with the IP method.

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Re: Ask Quick Questions, Get Quick Answers [Re: tryptkaloids] * 2
    #29256973 -

tryptkaloids said:
Mackload said:
two jars toque f8 colonizing extremely slowly, I thought totally stalled, but after a second shake (and warmer temps) suddenly took off, colonized the jars in two days, except for those clumps. probably bacteria? maybe top fruit?





That looks like killer spawn.



looking good after another shake, ty!



--------------------
Learn it, know it, live it


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Re: Ask Quick Questions, Get Quick Answers [Re: Axman] * 1
    #29256976 -

I tried using a proper sous vide with circulation and had similar result. Prob just need a bit more time or temp. 

I like making 250mL media bottles of agar in the IP too.  Makes an excellent hot water bath on sous vide mode to hold the agar as liquid after the PC cycle completes.  I fill the IP water to the same level as the agar in the media bottles.

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Re: Ask Quick Questions, Get Quick Answers [Re: Axman] * 1
    #29257002 -

humble suggestion to watch mycophilias instant pot video on youtube.  that will give you a general outline of the what/how with regards to instant pot mycology.

i prep my brown rice with my instant pot, but thats just 9 min in boiling water, cold rinse, strain, and into jars for a 135 min pressure cook in the IP.  I have no rye experience so I can't help you with specifics on that particular grain.

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Re: Ask Quick Questions, Get Quick Answers [Re: dinosaurs] * 2
    #29257037 -

A sous vide isnt the most even heating unless there's a good surface area. Try getting it well preheated before adding grain


--------------------
"Remember, kids, the difference between science and screwing around is writing it down" -adam savage
Flowchart for Recommended plan of action.
Learn the tried and true way to grow mushrooms
Use the Damn search engine
After you know what you're doing, take a break 
Pick a book, Make some chips!
Josex said:Don't take the site seriously bro, ain't worth it.
 

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Re: Ask Quick Questions, Get Quick Answers [Re: tryptkaloids]
    #29257105 -

Crossposting from the exotics thread. I'm now thinking that I may have posted in the wrong subforum, and there might be more people familiar with that species.

So, I've seen somewhere on the Shroomery that Psilocybe subaeruginosa is the same as Psilocybe semperviva. Like, "they're kind of Aussie semps". I need to clarify this.

I still doubt that this is true. I mean, I've always thought that this is a finicky woodlover that requires special cool conditions, and subaeruginosa is not well-suited for indoor growing. Is this debunked? I just ordered Psilocybe subaeruginosa from Australia, and I'm considering how to grow it. Will it work if I grow it the same way as Ps. semperviva indoors?

By the way, Is there an official thread for Ps. subaeruginosa? I couldn't find one.

Cheers.

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Re: Ask Quick Questions, Get Quick Answers [Re: tryptkaloids]
    #29257113 -

I use the saute option and I get a nice rolling boil. I don’t cover and boil what I’ve been using popcorn for an hour. Other grains may need more or less time though.

I have a separate pc I use for sterilization.i don’t recommend anyone use a instant pot for sterilization it’s not accurate enough / capable


--------------------
Face failure head on and learn something from it
The Hitchhiker's Guide to the Shroomery

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Re: Ask Quick Questions, Get Quick Answers [Re: misterswisher] * 3
    #29257120 -

Subs are woodlovers and semps arent. Woodlovers are best suited for outdoor cultivation.

Look in the woodlovers thread for more info


--------------------
"Remember, kids, the difference between science and screwing around is writing it down" -adam savage
Flowchart for Recommended plan of action.
Learn the tried and true way to grow mushrooms
Use the Damn search engine
After you know what you're doing, take a break 
Pick a book, Make some chips!
Josex said:Don't take the site seriously bro, ain't worth it.
 

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Re: Ask Quick Questions, Get Quick Answers [Re: Bra] * 3
    #29257124 -

Bra said:
Crossposting from the exotics thread. I'm now thinking that I may have posted in the wrong subforum, and there might be more people familiar with that species.

So, I've seen somewhere on the Shroomery that Psilocybe subaeruginosa is the same as Psilocybe semperviva. Like, "they're kind of Aussie semps". I need to clarify this.

I still doubt that this is true. I mean, I've always thought that this is a finicky woodlover that requires special cool conditions, and subaeruginosa is not well-suited for indoor growing. Is this debunked? I just ordered Psilocybe subaeruginosa from Australia, and I'm considering how to grow it. Will it work if I grow it the same way as Ps. semperviva indoors?

By the way, Is there an official thread for Ps. subaeruginosa? I couldn't find one.

Cheers.



Nope to all of that.  It’s a temperate woodlover and the parent species of cyans and azzies.  They are very robust and are almost exclusively grown outside on woody debris.

Subtropicalis/semps are a tropical/subtropical psilocybe from the new world.  Their growing conditions are near identical to cubes and they are typically grown indoors on coir.


--------------------
🅃🄴🄰🄼 🄸🄷🄾🄿


New to cultivation or the shroomery?  Observe the hitchhiker's guide

The Hitchhiker's Guide to the Shroomery



Smellyhobbit said:
"Wiping a needle with isopropyl after flame sterilization is like shitting after you wipe your ass."

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Shop: MagicBag.co All-In-One Bags That Don't Suck   Original Sensible Seeds Autoflowering Cannabis Seeds   Mushroom-Hut Substrate Mix   North Spore Injection Grain Bag   Sporeworks.EU Spores for European Microscopy   Myyco.com Isolated Cubensis Liquid Culture For Sale


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