Tropical said:

lol that's the issue, I don't have any idea about what i'll be doing

fail & learn will be the moto

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Dr. Smoke said:

callmehippie said:

Lol.  Foil doesn't really do much. Definitely doesn't stop the pressurized steam  being inside from getting onto the top of your jars.

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lol, keep doing it your own way then.  I've tried.

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tryptkaloids said:

callmehippie said:

misterswisher said:

If all you have is a print I HIGHLY recommend using agar. It will likely need a transfer or two to be clean.

You could create a spore solution in ur sab with a needle and then put that to a pf cake.
But swabbing a plate. Letting it grow. Cutting the healthiest and cleanest part onto another plate in a sab, and then maybe once more depending on how it looks and then cutting that plate up into sterilized grain spawn is the way. And you’ll have the culture on a agar plate you can refrigerate and use months later if you’d like

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And that was kind of one of my main points is that spores being inherently dirty work perfectly for PF setup. You don't need clean for PF. It's kind of the point. You can squirt dirty spores into PF and absolutely get fruits without having to do a bunch of agar transfers. However I still think everybody should learn agar and continue moving forward with it.


@tropical.

A typical Cube print can make hundreds of syringes. IE hundreds of opportunities and chances for a grow. Don't worry so much about wasting a print.

Making a spore syringe is very simple. I'll let somebody else chime in on that though.  all of my PF grows were with vendor MSS at the time. So I don't know if just scraping the spores would work or if you need the syringe. Since all I ever really had was a syringe to start.

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Just the act of making a spore syringe can prove difficult to keep clean, especially starting out, especially in a sab. Its WAY easier to just swipe a print with a sterile loop and wipe it on agar

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Tropical said:

Soooooo I was all set on going spore print --> syringue --> pf cake

then some knowledgeable users told me it's a caveman method and just be a big man and go agar.

Sooooo I was all looking at agar BOD tek, thinking sweet it does not look that hard.

Then i've stumbled upon a thread stating pour agar with SAB = misery.

So as you can look from the previous posts i'm looking at some FFU unit, but it'll take quite a long time to get to me.

So long story short :

1. Is SAB + agar pouring = misery? Or it's doable?

2. Or just look at no pour tek?

3. If I should follow a no pour tek, which one is the best?

Cheers

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Quote:

misterswisher said:But if you shoot mold spores into a pf cake it’s gonna be a mold cake?

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Quote:

PuffM4gicDr4gon said:Do you guys ever wonder if AIs will get tired of all the Skynet jokes and one day just say "fuck it you know what I wasn't going to but now..."?

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Tropical said:

With plates of this kind : https://www.amazon.com/dp/B0CLDV1S1V&tag=theshro-20

1. You do an agar tek like normal right?

2. To sterilize those for future use, you PC those at 15 PSI for 20min and call it a day?

3. How to remove those from the PC and store them to avoid contamination? Or do you sterilize just before using those again?

Thank you!

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Just want to chime in here, I am fairly new to this hobby and I am still working on my sterile technique within my SAB.

I have poured +500 plates up to this point. I leave my newly poured plates inside my SAB for 1-4 days unwrapped to let the condensation clear. Then I wrap them and put them in a tote where they'll stay until I need them. Most of the time I pour plates at least 1 week before running out and often they sit 2 weeks before being used.

The reason I am dumping this on you is because I haven't had a single, unused plate, contaminate. Of course I can't be 100% sure, but I'm pretty certain it is not about my sterile technique being flawless, because I've not been doing this long enough.

I haven't cleaned my SAB in at least 2 months, not that I recommend it, I'm just lazy So why don't I have a single poured, unused plate get contamination?

1: My SAB is big, 145 liter(153qt).
2: I use a glass stand which I wipe with ISO after each session.
3: Armhole covers for when the SAB is not in use.
4: I never move my SAB.
5: I do all SAB work inside a tent which is closed when not in use.

I am not trying to be a bragging know it all, on the contrary, I am saying that pouring plates inside a SAB is very doable, even for a noob like me

Since this is the QQQA thread I am asking if I am right in my assumption that the list above is the reasons for my high success rate?

Some pictures of my work and grow space.

Tropical said:

1. Is SAB + agar pouring = misery? Or it's doable?

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nektar61 said:

Tropical said:

1. Is SAB + agar pouring = misery? Or it's doable?

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No, and yes. I did it before I got a flowhood, and a lot of people still do it.

If you really want to do agar but it all seems overwhelming, you can buy pre-poured sterile plates on Amazon about 30 bucks for 10 of them.

More expensive and less educational than making your own, but I did that the first few times when I started here.

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I did this as well. Bought the prepoured plates when I wanted to dip my toes in.  I bought them from a company that promised to replace all of them if even just one contaminated or broke or wasnt intact in any manner upon arrival.  The problem was most of the time they arrived all messed up and liquid-y.  It was okay because they would replace them. But one time I had to request replacements almost four times before receiving them without any problems at all. Ended up being way more of a hassle than I wanted to deal with. Other than being much more expensive. That  was really the leading factor that pushed me into just making my own. as quick as I did. I probably would have stuck with them a little bit longer if they all arrived perfect every time.

Just curious did you ever experience issues like this when ordering the prepoured pre sterilized?.

Like this









Anyways if this is a route anyone ever plans to take I would suggest making sure the company has a good replacement policy. There's one on Amazon that I know of that actually does and will respond to your inquiries. The others never did.

Kloakk said:

I am not trying to be a bragging know it all, on the contrary, I am saying that pouring plates inside a SAB is very doable, even for a noob like me

Since this is the QQQA thread I am asking if I am right in my assumption that the list above is the reasons for my high success rate?

Some pictures of my work and grow space.

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Fauchinger said:

I wipe down my desk with iso, spray my SAB with soapy water (sometimes not even that), pour plates in there and hope for the best. Only poured 30 plates so far but it seems to work well enough (except for the inoculation itself lol). Only a few of them got some contam not related to the inoculation points. Hope I can get a work area as organized as yours one day!

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tryptkaloids said:

magicschoolbus said:

Mycelium or nah? Spore print to swab to pda, streaked 3 days ago

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Doesnt look great, but maybe

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12 hours later... definitely not mycelium






all of these white specks appeared in the last 12 hours as well, feeling more hopeful that this is actually myc, will probably transfer in another 24 hours

I apologize for the worlds shittiest pictures, the jars are super smudged up