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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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Re: Ask Quick Questions, Get Quick Answers [Re: Tropical] * 1
    #29241625 -

Tropical said:
Hi,

Anyone knows if psylocibilin is distributed evenly inside the shroom or there is more in the stem or cap?


Cheers


Its homogeneous (even distribution)


--------------------
"Remember, kids, the difference between science and screwing around is writing it down" -adam savage
Flowchart for Recommended plan of action.
Learn the tried and true way to grow mushrooms
Use the Damn search engine
After you know what you're doing, take a break 
Pick a book, Make some chips!
Josex said:Don't take the site seriously bro, ain't worth it.
 

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Re: Ask Quick Questions, Get Quick Answers [Re: milkboy] * 2
    #29241656 -

milkboy said:
Nichrome said:
milkboy said:
Nichrome said:
The contest is annoying. It is an ego contest and is especially annoying in this thread.

I mean posing an opening to get it going again as a qq is clever I'll give you that. But let's not get it going again... let the scab heal.

Understandably so, it is easy to get drawn into argumentation as a conversational modality. The human condition is rooted in fight or flight and once drawn into such an exchange, it can be difficult to escape from without hurt feelings on all sides. This is water for chocolate and I invite anyone struggling with that feeling of need in argumentation to click the word "arguments" in my current sig for some ideas about how to converse and discuss without the use of arguments. 

Here is a link to the guidelines which state the intent and dynamic this thread was made to embody, as a good learning place. It seems like some people could use a refresher.

Sorry I haven't been around much. I love you all.


I accept you apology conditionally. You must stay



Conditionally, it's a done deal. But first you must answer for me these questions three.

1) Using wild sourced organisms, how would you go about stimulating mycelium in a heterotrophic growth phase, into a chemoautotrophic growth phase?

2) What target genus or species would you choose for such a process, and why?

3) What might this be useful for?


:popcorn:


1. By feeding it a appropriate organic carbon source until ready to transition it to an inorganic source, or use  a GEM...

2. C. necator because of its ability to quickly switch phases in around 12 hours

3.Industrial production of things like Astaxanthin produced from microalgae, by building up a large biomass before transitioning to its chemoautotrophic phase to produce the Astaxanthin in greater quantities?

Even if I got some of this wrong, I smell what you are cooking :popcorn:


There are no wrong answers to those questions, although I think your answers are of a unique and high quality, indicative of you depth of understanding.

I especially appreciate you narrowing it down to a single species of interest, with valid reason.

Done deal.


--------------------
Question everything, it's very responsive.

Culture Storage

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Re: Ask Quick Questions, Get Quick Answers [Re: tryptkaloids]
    #29241681 -

tryptkaloids said:
Youd think so but no, you can rehydrate it and it will come back to life. Faht and many others like to spread cultures on dried grain.

But dried tissue can revive. Its what sclerotia specialized specifically to make easier.

https://www.shroomery.org/forums/showflat.php/Number/27027851#27027851




The only thing is once it has been dried. The likelihood that you're actually getting a pure clone as opposed to possibly gettung multiple genetics from some spores that could have been dumped on the tissue is much less likely I believe.

So yes it can be revived but how certain of it are we that it is actually 100% clean "cloned".

And not ms


--------------------
:hippie::mushroom2:Take it easy. if it's easy. take it twice:mushroom2::mushroom2:
:sporedrop:The Hitchhiker's Guide to the Shroomery:sporedrop:

**A LINK TO EVERTHING**


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Re: Ask Quick Questions, Get Quick Answers [Re: Tropical]
    #29241710 -

Tropical said:
Hi,

Anyone knows if psylocibilin is distributed evenly inside the shroom or there is more in the stem or cap?

Cheers


I have wondered this and don't know for sure. But I can say from bioanalysis (eating shrooms) it's not vastly different.

If it is different it's probably 1.1 to 1, not 2:1 or even 1.5 to 1.

In the 80s when we were picking shrooms in cow fields in Texas, the common belief was "Only the caps are good, throw out the stems", one day I ate only stems and proved that bullshit wrong.

Some people said "stems have no drug", other people said "stems are toxic" haha, neither are true.

Either way they were throwing away pounds of perfectly good stems.


--------------------
--How to grow POPPIES.
--GROWING SHROOMS, FROM SPORES TO TRIPS
--x7x+ variety history.

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Re: Ask Quick Questions, Get Quick Answers [Re: nektar61]
    #29241716 -

What do we do about over hydrated coir?

Squeezing it to field capacity is killing my hands.. it’s 6 bricks worth of coir 😂😭

At first it was slightly under field capacity so I added another 2 quarts of hot water. I check back after a few hours and now it’s very much over field capacity. Still looks right if that makes any sense but you get a good amount of water when you squeeze it


--------------------
Face failure head on and learn something from it
The Hitchhiker's Guide to the Shroomery

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Re: Ask Quick Questions, Get Quick Answers [Re: misterswisher] * 1
    #29241718 -

misterswisher said:
What do we do about over hydrated coir?



add a little extra verm until it's at FC.

But I'd mix in the spawn first.


--------------------
--How to grow POPPIES.
--GROWING SHROOMS, FROM SPORES TO TRIPS
--x7x+ variety history.

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Re: Ask Quick Questions, Get Quick Answers [Re: tryptkaloids]
    #29241735 -

tryptkaloids said:
Youd think so but no, you can rehydrate it and it will come back to life. Faht and many others like to spread cultures on dried grain.

But dried tissue can revive. Its what sclerotia specialized specifically to make easier.

https://www.shroomery.org/forums/showflat.php/Number/27027851#27027851


As I understand it the “dry” myceliated grain isn’t completely dead-dry. I don’t think bone dry mycelium or tissue can be resurrected.

Especially considering the temperatures used in dehydrating fruits


--------------------
Thank your postal workers when you see them. None of this would be possible without them.

Necessity is the mother of invention

Edited by EniQma (06/06/25 10:12 PM)

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Re: Ask Quick Questions, Get Quick Answers [Re: ThurmanMurman] * 1
    #29241741 -



Is this huge angle hair mass fungus or mycelium?  There appears to be myc showing on the left and in layers viewing from the side. Oh, it smells like really ripe bananas.

Can this be saved?

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Re: Ask Quick Questions, Get Quick Answers [Re: MN_John] * 3
    #29241744 -

MN_John said:
Can this be saved?


:ohmy:


--------------------
🅃 🄴 🄰 🄼    🄲 🄻 🄸 🄽 🄶 🅆 🅁 🄰 🄿

HitchHiker's Guide
Identifying proper surface conditions

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Re: Ask Quick Questions, Get Quick Answers [Re: MN_John]
    #29241750 -

MN_John said:


Is this huge angle hair mass fungus or mycelium?  There appears to be myc showing on the left and in layers viewing from the side. Oh, it smells like really ripe bananas.

Can this be saved?


I'd get that the hell out of my house and start over. Really ripe bananas? Never smelled shrooms like that, and that looks like mold.

Cannot be saved, nor should one try.

Read some teks to start over with better tek.

Tell here how you did your agar and jars.


--------------------
--How to grow POPPIES.
--GROWING SHROOMS, FROM SPORES TO TRIPS
--x7x+ variety history.

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Re: Ask Quick Questions, Get Quick Answers [Re: EniQma]
    #29241762 -

EniQma said:
tryptkaloids said:
Youd think so but no, you can rehydrate it and it will come back to life. Faht and many others like to spread cultures on dried grain.

But dried tissue can revive. Its what sclerotia specialized specifically to make easier.

https://www.shroomery.org/forums/showflat.php/Number/27027851#27027851


As I understand it the “dry” myceliated grain isn’t completely dead-dry. I don’t think bone dry mycelium or tissue can be resurrected.

Especially considering the temperatures used in dehydrating fruits



It's definitely been done with the bone dry straight out of the dehydrator tissue.  Not just grain. . Just basically rehydrated and that's all it takes. Just the likelihood of it being a true clone isn't very likely.  considering it probably has a lot of spores on it as well and who's to say those didn't just germinate in the process.


--------------------
:hippie::mushroom2:Take it easy. if it's easy. take it twice:mushroom2::mushroom2:
:sporedrop:The Hitchhiker's Guide to the Shroomery:sporedrop:

**A LINK TO EVERTHING**


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Re: Ask Quick Questions, Get Quick Answers [Re: EniQma]
    #29241787 -

Actually I recently had a dime bag with like a strand of mycelium dried up on the side from being shipped something. Almost a year ago at this point.

Anyway I scrapped it into a dish and put a piece of agar on top. was thinking it could help rehydrate it. Didn’t have growth for the first week but by day 8-9 it’s showing signs of growth.

In my case I don’t think it was 100% dehydrated. But I wouldn’t put it past dried up mycelium from springing back to life. There’s a scientific term for it,  chatgpt or the internet will explain it better than me, but it’s when cells can essentially be dead, but something can trigger the cells to split and find life again. Idk I’m baked. But the shrooms are magic dude. Don’t put anything past em


--------------------
Face failure head on and learn something from it
The Hitchhiker's Guide to the Shroomery

Edited by misterswisher (06/06/25 11:58 PM)

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Re: Ask Quick Questions, Get Quick Answers [Re: MN_John]
    #29241791 -

MN_John said:


Is this huge angle hair mass fungus or mycelium?  There appears to be myc showing on the left and in layers viewing from the side. Oh, it smells like really ripe bananas.

Can this be saved?


You can't save that, but you can save yourself! Just take it outside and let it go into the sun. If you're up for sharing how you ended up here, we will try to help you figure out what went right and what didn't, so you can learn from it.

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Re: Ask Quick Questions, Get Quick Answers [Re: Arkyhog]
    #29241792 -

Thats one of the most clear cases of cobweb mold ive ever seen


--------------------
"Remember, kids, the difference between science and screwing around is writing it down" -adam savage
Flowchart for Recommended plan of action.
Learn the tried and true way to grow mushrooms
Use the Damn search engine
After you know what you're doing, take a break 
Pick a book, Make some chips!
Josex said:Don't take the site seriously bro, ain't worth it.
 

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Re: Ask Quick Questions, Get Quick Answers [Re: Arkyhog]
    #29241793 -

I want to start getting into weaning down MS genetics with the goal of getting full canopies.

What's the best way to go about this? I'm guessing clone clusters and grow them out, but I'm unsure where to go from there.


--------------------
I've got skills, they don't know where to use me
I'm like the best dressed guy at the nude beach
Nice to meet you!

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Re: Ask Quick Questions, Get Quick Answers [Re: PuffM4gicDr4gon] * 1
    #29241794 -

That’s basically it


--------------------
A Love Letter to New Growers
The 7 Edicts of Mushroom Cultivation as Decreed by Smellyhobbit

:kodama:


Anyone can get a canopy. But can you get what you want?

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Re: Ask Quick Questions, Get Quick Answers [Re: Smellyhobbit]
    #29241795 -

You just clone fruits that look good to you and if they grow a canopy you keep it. If it doesn’t, try again.


--------------------
A Love Letter to New Growers
The 7 Edicts of Mushroom Cultivation as Decreed by Smellyhobbit

:kodama:


Anyone can get a canopy. But can you get what you want?

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Re: Ask Quick Questions, Get Quick Answers [Re: Smellyhobbit] * 1
    #29241797 -

Do I need to go to spore afterwards, or do I clone the clone?


--------------------
I've got skills, they don't know where to use me
I'm like the best dressed guy at the nude beach
Nice to meet you!

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Re: Ask Quick Questions, Get Quick Answers [Re: PuffM4gicDr4gon]
    #29241800 -

PuffM4gicDr4gon said:
Do I need to go to spore afterwards, or do I clone the clone?


Thanks for asking this.  I was literally off searching the same after reading the prior exchange.


--------------------
The Hitchhiker’s Guide to the Shroomery - BeefSupremeJr
A Love Letter to New Growers - Smellyhobbit

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Re: Ask Quick Questions, Get Quick Answers [Re: PuffM4gicDr4gon] * 6
    #29241802 -

PuffM4gicDr4gon said:
Do I need to go to spore afterwards, or do I clone the clone?


Depends on your goals, I’d think.

For reference, I’m still growing clones I took in 2022/early 2023. I just started working with the first clone I ever took again thanks to a friend who had it in storage this whole time.

If you have a culture that performs really well and that’s your goal, just take steps to preserve that as well as possible. If you want to continue working from spore, do that. It’s usually a good idea to take spores when you’ve got good genes on your hands in case you need them, but if your clone is handled correctly you can make hundreds of tubs off 1 master plate.

My Vader clone was kept in cold storage. Id take a little .5cm square sample, expand to a new plate, and grow off that for months. Go back and take another whenever that plate ran out.


--------------------
A Love Letter to New Growers
The 7 Edicts of Mushroom Cultivation as Decreed by Smellyhobbit

:kodama:


Anyone can get a canopy. But can you get what you want?

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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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