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Re: Ask Quick Questions, Get Quick Answers [Re: wavyedge]
    #29232033 -

callmehippie said:
Are you sure? . It was May 28th 2 somthin in the morning here whenever he said that. I don't know how it works but if I registered on the may 28th 2024 and it was/is the  28th 2025 now.  I believe today was the anniversary. I don't recall the site notifying me. If it weren't for DK I would've had no clue LOL


I was going to say maybe it's a time zone or part of the world type of thing. Except for I'm in the US and he's in New Zealand so I guess that doesn't work LOL


Anyways I got the 1000




I am still confused :lol:, but congratulations :awesomenod:


wavyedge said:
I'm betting the registration "date" is a timestamp that in your time zone equals that date, while in their own it's the 28th. UTC time stamp + your local offset, truncating the actual time.

But which time zone is the real time zone? Programming with dates sucks.





The thing that confused me is that in my time zone shroomery know it's his anniversary because there is a little shroom next to the registration date, but it still says it's the 29th.. :lol:

But yeah, might just be the programming.


--------------------
“All Fungi are edible.

Some fungi are only edible once.”
― Terry Pratchett


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Re: Ask Quick Questions, Get Quick Answers [Re: dinosaurs]
    #29232036 -

Are these little yellow dots bacterial contamination or are they slime mold or something similar?



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Re: Ask Quick Questions, Get Quick Answers [Re: SkanskKorv] * 1
    #29232054 -

Bacteria. Looks like it ran down the plate when you transferred. You can see where the mycelium grew over it at 2 and 5 o’clock.

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Re: Ask Quick Questions, Get Quick Answers [Re: The Tao]
    #29232057 -

Yeah I noticed it looks like it had been poured over the plate, I probably brought something in by the condensation on the top plate which I've been struggling with.


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Re: Ask Quick Questions, Get Quick Answers [Re: Nichrome]
    #29232071 -

Contam? Yellow Dots one little one and a bigger one in my Tub after 1-2 weeks of colonizing first pins are forming is it contam? If yes will i be able to collect the first flush?

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Re: Ask Quick Questions, Get Quick Answers [Re: SkanskKorv]
    #29232072 -

Jrey16 said:
Can anybody point me in the right direction on how to grow P. nats? Any good threads out there like they have for growing Pan Cyan? I have a few P nat jars almost fully colonized and will be spawning to bulk soon. According to what I've read you can grow them exactly like cubes in monotubs, but they just need more FAE. Some saying 5x more FAE. Is there anything else I'm missing?



If you are talking about actual nats I have no idea, but if you are talking ochraceocentrata that we used to call natalensis you are on the right track. I grew them just like I grow cubes. 1:5 spawn:substrate ratio with plenty of passive air exchange.

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Re: Ask Quick Questions, Get Quick Answers [Re: RasenderRon] * 2
    #29232092 -

RasenderRon said:
Contam? Yellow Dots one little one and a bigger one in my Tub after 1-2 weeks of colonizing first pins are forming is it contam? If yes will i be able to collect the first flush?





Side pins.

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Re: Ask Quick Questions, Get Quick Answers [Re: MycCycle] * 1
    #29232098 -

MycCycle said:
RasenderRon said:
Contam? Yellow Dots one little one and a bigger one in my Tub after 1-2 weeks of colonizing first pins are forming is it contam? If yes will i be able to collect the first flush?





Side pins.



:whathesaid:

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Re: Ask Quick Questions, Get Quick Answers [Re: MycCycle]
    #29232099 -

Have any of you used individually wrapped sterile medical gauze sponges to wipe moisture or condensation from hot poured agar plate lids a couple days after your pour and before you wrap?

I have used paper towels sprayed lightly with iso and haven’t had any contamination issues at all on new plates. But wondering if sterile gauze would be a better option.

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Re: Ask Quick Questions, Get Quick Answers [Re: AZ Starman2112] * 2
    #29232109 -

AZ Starman2112 said:
Have any of you used individually wrapped sterile medical gauze sponges to wipe moisture or condensation from hot poured agar plate lids a couple days after your pour and before you wrap?

I have used paper towels sprayed lightly with iso and haven’t had any contamination issues at all on new plates. But wondering if sterile gauze would be a better option.



not a good idea, neither nor. its a vector of contam that can be totally avoided.


--------------------
the observer is the observed
            j. krishnamurti


>The Hitchhiker‘s Guide to the Shroomery<

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Re: Ask Quick Questions, Get Quick Answers [Re: AZ Starman2112] * 1
    #29232114 -

AZ Starman2112 said:
Have any of you used individually wrapped sterile medical gauze sponges to wipe moisture or condensation from hot poured agar plate lids a couple days after your pour and before you wrap?

I have used paper towels sprayed lightly with iso and haven’t had any contamination issues at all on new plates. But wondering if sterile gauze would be a better option.



I utilize round PP5 bead containers for no-pour agar. To maintain lid visibility I always wipe the water off my lids using a paper towel. I also soak a folded paper towel in 70% isopropyl alcohol and place my agar container face down on it while I clean the lid. This process is always carried out in front of a filtered airflow unit (FFU) to ensure a sterile environment. Additionally, I store the containers upside down during their initial use to prevent any moisture from accumulating on the agar. Whenever I handle agar, I make sure to wear gloves and a mask while working in front of the FFU. So far, I've only needed to discard a few plates due to contamination from my runs, and I typically process about 30 plates at a time.





Edited by Arkyhog (05/29/25 08:20 AM)

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Re: Ask Quick Questions, Get Quick Answers [Re: Arkyhog]
    #29232135 -

chris77 said:
not a good idea, neither nor. its a vector of contam that can be totally avoided.



I understand the risks. But after making a couple hundred plates, I’ve had only two I had to toss that became contaminated weeks later. I have dried quite a lot of them the way I described. To me the risk of wrapping a plate with condensation is riskier than drying the lid properly in a SAB.


Arkyhog said:
I utilize round PP5 bead containers for no-pour agar. To maintain lid visibility I always wipe the water off my lids using a paper towel. I also soak a folded paper towel in 70% isopropyl alcohol and place my agar container face down on it while I clean the lid. This process is always carried out in front of a filtered airflow unit (FFU) to ensure a sterile environment. Additionally, I store the containers upside down during their initial use to prevent any moisture from accumulating on the agar. Whenever I handle agar, I make sure to wear gloves and a mask while working in front of the FFU. So far, I've only needed to discard a few plates due to contamination from my runs, and I typically process about 30 plates at a time.




I do it pretty much how you do it, upside down but in a SAB and I mist the paper towel with iso instead of soaking it making it sanitized but still absorbent.

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Re: Ask Quick Questions, Get Quick Answers [Re: Jrey16] * 2
    #29232157 -

Jrey16 said:
Been having an ant problem lately and noticed there was a few tiny ants on the inside of a couple of monotubs that are still colonizing. I didn't notice any ants on the substrate, but there were a few ants crawling on the walls of the inside of the monotubs. Will that be a problem?



It's probably nothing to worry about, unless they go for the grain. They're probably just checking things out. If the form bold lines and are carrying food out of your tub then maybe worry.

There are about 200 species of ants that are known to eat fungus. There are about 14,000 known species of ants out there. So probability wise you're in a good spot. If you really are in Philippines you are probably more likely to encounter some fungus eating ants. But hey we've all gotta eat right? Keep an eye on them, I would love to know what they do.



Fun picture of some ants from southeast Asia eating some oysters:

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Re: Ask Quick Questions, Get Quick Answers [Re: sprnk]
    #29232159 -

The peat moss I usually use to case pan cyan is out of stock and I can only find peat moss that says it has added fertilizer in it. Is that ok to use for a 50/50 peat verm casing layer?


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Re: Ask Quick Questions, Get Quick Answers [Re: AZ Starman2112] * 2
    #29232169 -

AZ Starman2112 said:
Have any of you used individually wrapped sterile medical gauze sponges to wipe moisture or condensation from hot poured agar plate lids a couple days after your pour and before you wrap?

I have used paper towels sprayed lightly with iso and haven’t had any contamination issues at all on new plates. But wondering if sterile gauze would be a better option.



When I do Primo Plates I end up with 2x pieces of paper towel per set of 10x plates and since those ran through the PC cycle I believe they are sterile enough to wipe down the plate lids IMHO.

I usually prep 40-80 plates at a time, then I'll clean the lids of condensation, and put them back in the jar with a modified lid, and put them on the shelf to sit for literally months before I use them sometimes. Since I don't wrap them and there is some gas exchange inside the jar the plates are usually nice and ready for use whenever I need them.

Out of 500 plates I've maybe had 5 contaminate while waiting to be inoculated, that's something I'm willing to live with.

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Re: Ask Quick Questions, Get Quick Answers [Re: Axman] * 1
    #29232186 -

Axman said:
When I do Primo Plates I end up with 2x pieces of paper towel per set of 10x plates and since those ran through the PC cycle I believe they are sterile enough to wipe down the plate lids IMHO.

I usually prep 40-80 plates at a time, then I'll clean the lids of condensation, and put them back in the jar with a modified lid, and put them on the shelf to sit for literally months before I use them sometimes. Since I don't wrap them and there is some gas exchange inside the jar the plates are usually nice and ready for use whenever I need them.

Out of 500 plates I've maybe had 5 contaminate while waiting to be inoculated, that's something I'm willing to live with.



Great info Ax! I like Rev’s tek and almost went with that, but opted for D3’s instead. I think they are both great teks.

Like you I’m willing to live with the ratio of uninoculated plates I lost to contam from what I didn’t. It’s like 1% for you and me. Can one even contribute doing what we do to the plates lost? Maybe, but maybe not. It could have literally been anything, a flake of skin, a small hair, who knows. I feel comfortable knowing you and Arkyhog are doing pretty much the same as me with the condensation. I pour hot, so condensation is unavoidable. I stack tall now so it is less plates with condensation now, but it is still there. This week due to time constraints, I poured two batches as soon as the PC was cool enough to open. I wore gloves with paper towels inside them and wrapped a paper towel soaked in iso around the neck of the media bottles. Didn’t burn myself, but there is extra condensation in the plates as a result. I did order a box of 50 individually wrapped 4x4 bandaid brand sterile gauze sponges for $7. If they are good enough for surgery and medical uses, then they are good enough for Petri dishes imho.

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Re: Ask Quick Questions, Get Quick Answers [Re: Kloakk] * 2
    #29232208 -

Kloakk said:
LewDoja said:
Where's your vpn server? :shrug:




I don't have one :uhoh:



PSA - Here is some good info about which VPN to choose. If you see your VPN provider in the list of ones owned by huge companies, you should switch immediately. My choice is Mullvad, 5 euros/month, no logs whatsoever.


--------------------
"For the powerful freedom-takers who rob justice from us all, that's why I keep my pistol loaded, powder, cap, and ball..."
Read more, post less!
...turns out, I'm an amateur at everything

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Re: Ask Quick Questions, Get Quick Answers [Re: KlarnEvil9]
    #29232242 -

KlarnEvil9 said:
PSA - Here is some good info about which VPN to choose. If you see your VPN provider in the list of ones owned by huge companies, you should switch immediately. My choice is Mullvad, 5 euros/month, no logs whatsoever.



Thanks, I'll take a look :hatsoff:


--------------------
“All Fungi are edible.

Some fungi are only edible once.”
― Terry Pratchett


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Re: Ask Quick Questions, Get Quick Answers [Re: Jrey16]
    #29232378 -

Jrey16 said:
Been having an ant problem lately and noticed there was a few tiny ants on the inside of a couple of monotubs that are still colonizing. I didn't notice any ants on the substrate, but there were a few ants crawling on the walls of the inside of the monotubs. Will that be a problem?



Ants seek out moisture, that's why it's common to find them in the bathroom even though there's no food there.  I don't think they will colonize a tub, but I don't see many scenarios where their presence turns out positive.


--------------------
🅃🄴🄰🄼 🄸🄷🄾🄿


New to cultivation or the shroomery?  Observe the hitchhiker's guide

The Hitchhiker's Guide to the Shroomery



Smellyhobbit said:
"Wiping a needle with isopropyl after flame sterilization is like shitting after you wipe your ass."

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Re: Ask Quick Questions, Get Quick Answers [Re: Kloakk] * 2
    #29232384 -

Kloakk said:
KlarnEvil9 said:
PSA - Here is some good info about which VPN to choose. If you see your VPN provider in the list of ones owned by huge companies, you should switch immediately. My choice is Mullvad, 5 euros/month, no logs whatsoever.



Thanks, I'll take a look :hatsoff:



I'll add: if a VPN is free, it's probably a honeypot.

If it advertises on hundreds of YouTube channels, they probably hand over info to law endorsement with a request, not even requiring a warrant.


--------------------
--How to grow POPPIES.
--GROWING SHROOMS, FROM SPORES TO TRIPS
--x7x+ variety history.

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