AZ Starman2112 said:

Need help with these TTBVI plates. Every time I transfer to new plates, the plates start out great, but before they fully colonize literally overnight the plates look like these in my photo. The edges seem to dry out and look weird. I don’t think it is contamination, but I could be wrong. When I open to take transfers, the plates smell like healthy mycelium or mushrooms, so I know that is a good sign. Is this normal looking for TTBVI or not? Also I am using 16g LME, 20g agar and 1000ml water to make my plates. Any help or insight would be greatly appreciated!!!

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Puduwoke said:

Any thoughts on how well this would work to heat of my tent during the winter?

Have you seen anyone use something like this?

https://www.amazon.com/HYDGOOHO-Thermostat-Fahrenheit-Germination-MET-Certified/dp/B082VQNVC4/ref=mp_s_a_1_16_sspa?crid=2T8MFUYXMPKIG&dib=eyJ2IjoiMSJ9.Tlzm4pR3Tx-Etmrvn_JBNXs-hz_dziJ5QF4KKMH51FT-GMzwPvghwpdbPIF26nqUxBw_ZcO_Qt-K3Ji5bOA6TW4LuzJ2KTIOJ8bNzXTelJypWcUyLKLwvANjhsS6rw8Hl4o-HMraNVgP7QWqeSya3Ri_9cPeuo4quuShOS3DJJGES87N_hqzX5dVz8yDW0plNN-BEfVfv-ag2721htZ0Xw.QngH5H-xoN5wVL7gL6TeMEExOUUSdyGZ0nnKShJoyik&dib_tag=se&keywords=heating+mat&qid=1747950444&sprefix=heating+mat%2Caps%2C208&sr=8-16-spons&xpid=eZb_ha-Doo8CP&sp_csd=d2lkZ2V0TmFtZT1zcF9waG9uZV9zZWFyY2hfYXRmX25leHQ&psc=1

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Puduwoke said:

Edit: Does bisporus grow noticeably slower on agar than pan cyans? Mine seems to so thought I would ask

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Dr. Smoke said:

AZ Starman2112 said:

Need help with these TTBVI plates. Every time I transfer to new plates, the plates start out great, but before they fully colonize literally overnight the plates look like these in my photo. The edges seem to dry out and look weird. I don’t think it is contamination, but I could be wrong. When I open to take transfers, the plates smell like healthy mycelium or mushrooms, so I know that is a good sign. Is this normal looking for TTBVI or not? Also I am using 16g LME, 20g agar and 1000ml water to make my plates. Any help or insight would be greatly appreciated!!!

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I'm not a doctor but I play one on TV.  I would call those 'stalled out'.  The agar may be too thin dimensionally, or may have been too diluted. 

Also, Pans need extra nitrogen to thrive, and that means agar with peptone.

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AZ Starman2112 said:

Thanks for the info Doc. I don’t have peptone in my agar plates, but the recipe for these is 16g LME, 20g agar & 1000ml water. These plates have been hosting pretty much everything I’m doing at the moment, and Pan Cyan Peace River, Ochra/Nats & P Mex Galindoi are the other things I’ve got on them besides cubes. Those are all doing great. It’s just the TTBVI that I keep running into this stall. Someone said cut my LME in half and someone else mentioned not to let the plates go much bigger than a quarter or half dollar in size and then take either more transfers or if clean drop to grain. Any suggestions on the info I was given or should I just add peptone to my recipe, and how much? TIA!

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I use the exact same recipe(2% agar agar 1,6% DME) as you and use it for everything, well I also have some water agar plates. I am using distilled water and pans look like this.






I agree that a little nitrogen doesn't hurt anything and might be worth a shot, but certainly not needed.

Dkeppel said:

@Kloakk and @AZ Starman2112

Your over thinking it guys. Don't worry about nitrogen or whatever, try half the nutes.

0.5 to 1% is what is recommend for pans, trust me it will make your plates easier to read

Edit: Sorry Kloakk, your shits clean! Low nutes will help thin growth out and keep it organized if you do have any problems with weird growth, stalling etc on pan plates

AZ Starman2112 try the low nutrient agar, if that doesn't help I'd start again.

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Thanks for checking out my shit, glad it got cleared. And I have done a lot of 0,5% plates for pans, of course I'm always on that more for less train and like to avoid doing several agar recipes, but I've also found that using the 1,6% makes it easier to spot overlaying pan mycelium.

I also have some 1,6% DME plates with fermaid O(organic nitrogen from yeast autolysis) just to see if it made a difference, and I am now using it to help weak mycelium get an upper hand, I have no noteworthy yet, but it might be useful.

No fermaid O


With fermaid O

Dkeppel said:

How embarrassing

No one needs to check your plates, I didn't even blow them up!

I added the edit part because I was worried you might think I was implying something was wrong with the plates.

I see the name Kloakk and I expect perfection!

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What was the line again Aahhh yes now I remember "I'm having you on a bit"


In all seriousness tho, I always feel better when someone I really respect(like you) in these halls confirms my shit so keep it coming There is nothing perfect about anything I do and I am known for overthinking all the time, but I try not to get in my own way or be sloppy and so far it's saved me a lot of headache I think

Kloakk said:

Dkeppel said:

@Kloakk and @AZ Starman2112

Your over thinking it guys. Don't worry about nitrogen or whatever, try half the nutes.

0.5 to 1% is what is recommend for pans, trust me it will make your plates easier to read

Edit: Sorry Kloakk, your shits clean! Low nutes will help thin growth out and keep it organized if you do have any problems with weird growth, stalling etc on pan plates

AZ Starman2112 try the low nutrient agar, if that doesn't help I'd start again.

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Thanks for checking out my shit, glad it got cleared. And I have done a lot of 0,5% plates for pans, of course I'm always on that more for less train and like to avoid doing several agar recipes, but I've also found that using the 1,6% makes it easier to spot overlaying pan mycelium.

I also have some 1,6% DME plates with fermaid O(organic nitrogen from yeast autolysis) just to see if it made a difference, and I am now using it to help weak mycelium get an upper hand, I have no noteworthy yet, but it might be useful.

No fermaid O


With fermaid O

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  ik this the pan thread but i love that culture.. just revived it myself and its almost through a CS for good measure

fahtster said:

  ik this the pan thread but i love that culture.. just revived it myself and its almost through a CS for good measure

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AZ Starman2112 said:

@kloakk - I looked up the fermaid o. It seems like it’s a brewers yeast. I bought a bag of Anthony’s nutritional yeast flakes, but haven’t added it to any of my agar batches yet. Do you think that it could also be used in place of the fermaid o? How much were you adding to each batch since you are using the same recipe as me. Credit out to D3 for the agar recipe!

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Kloakk said:

AZ Starman2112 said:

@kloakk - I looked up the fermaid o. It seems like it’s a brewers yeast. I bought a bag of Anthony’s nutritional yeast flakes, but haven’t added it to any of my agar batches yet. Do you think that it could also be used in place of the fermaid o? How much were you adding to each batch since you are using the same recipe as me. Credit out to D3 for the agar recipe!

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Yes, nutritional yeast is made the same way fermaid O is, also with brewer yeast(saccharomyces cerevisiae) so you should be good to use that, I haven't used it in low nutrient plates or with pans, but it could be worth to test it. With fermaid O I use 1g/1000ml.

After some searching on here it seems' a lot of folks do the same with nutritional yeast, but ask in the qq thread to be sure, because some use 2g/1000ml.

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AZ Starman2112 said:

D3’s tek did mention 1g per batch. I had a feeling that is what you were using. Don’t mind me for double checking. And yes, assuming that for pans we would go lower nutes, I’d also assume that we wouldn’t want to add more nutes, if we are taking away. Given that adding only 1g of yeast to a batch of agar, it is probably pretty strong stuff.

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