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Offlineflannel
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Registered: 01/12/20
Posts: 19
Last seen: 1 month, 21 days
Re: Cultivating Panaeolus cyanescens [Re: Raxo]
    #27508443 - 10/17/21 08:30 PM (2 years, 3 months ago)





Do any of these have the phenotype we're looking for?  To me it seems like the right side of right colony in the bottom photo might be good


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OfflineAsuraS
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Registered: 08/01/11
Posts: 5,047
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Re: Cultivating Panaeolus cyanescens [Re: flannel]
    #27508814 - 10/18/21 09:13 AM (2 years, 3 months ago)

None of those look particularly healthy to me.


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OfflineHindsight
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Registered: 01/24/21
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Re: Cultivating Panaeolus cyanescens [Re: Asura]
    #27509063 - 10/18/21 01:32 PM (2 years, 3 months ago)

Asura, have you seen pan mycelium like this before?




This is what I am used to seeing for my pan mycelium but I also have been growing just one variety until a couple of weeks ago when I germinated like 6 new varieties. The pic above is one of the new varieties.


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OfflineAsuraS
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Re: Cultivating Panaeolus cyanescens [Re: Hindsight]
    #27509163 - 10/18/21 03:19 PM (2 years, 3 months ago)

Yeah, I've had plates like that first one more than a few times. I don't have scope so I don't
know anything for certain there...but I toss those. Second plate is textbook.


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OfflineHindsight
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Re: Cultivating Panaeolus cyanescens [Re: Asura]
    #27509187 - 10/18/21 03:40 PM (2 years, 3 months ago)

Thanks for the reply. Darn! I had already put it to grain yesterday though, so it will be interesting to see what happens.

I did recently get a scope but don't know what I'm looking at really. I've looked at mold from an isolated mold spot on a plate, and I've looked at clean pan and cube mycelium, and they all look about the same to me at 1000x.


Edited by Hindsight (10/18/21 03:41 PM)


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OfflineHindsight
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Re: Cultivating Panaeolus cyanescens [Re: Hindsight]
    #27511069 - 10/20/21 08:02 AM (2 years, 3 months ago)

Any reason you like to colonize at cooler temps of 72? Is it to help keep it more contamination resistant?


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OfflineAsuraS
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Re: Cultivating Panaeolus cyanescens [Re: Hindsight] * 1
    #27511221 - 10/20/21 10:38 AM (2 years, 3 months ago)

No reason at all other than it's easy. It works and it's one less thing
to worry about before fruiting.


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OfflineHindsight
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Re: Cultivating Panaeolus cyanescens [Re: Asura]
    #27511222 - 10/20/21 10:38 AM (2 years, 3 months ago)

Thanks!


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InvisibleUrbanCubensis


Registered: 05/13/18
Posts: 172
Loc: down the road
Re: Cultivating Panaeolus cyanescens [Re: Hindsight]
    #27514154 - 10/22/21 07:06 PM (2 years, 3 months ago)

Hey finally getting around to revamping an old pan. cyan. jamaican print, tried spores > agar nothing has shown after 3 weeks, the old fully colonised plate is likely from mid 2019 just haven't gotten around to cleaning it up. Originally I thought the blue veins may have been bruising however the recent one taken ~4 days ago is coming up as a flaky blue looking exactly like contam, however there is wispy white growing away from it in a perfect circle, unsure if its clean or not.

Any idea of where to maybe take a sample off the full plate? Or would the contam have colonised over the entire mycelium by now.

Thanks! Would love to get some pans going again just this old print has been extremely stubborn to get any growth (mid-2019) Have tried scraping literal clumps of spores onto plates however nothing is growing not even contam.



Regards,
UrbanCube.


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InvisibleBaba Yaga
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Registered: 09/13/20
Posts: 3,955
Loc: Hyperspace Chicken Coop
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Re: Cultivating Panaeolus cyanescens [Re: UrbanCubensis]
    #27514386 - 10/22/21 09:22 PM (2 years, 3 months ago)

That doesn't look good, you're better off giving your spores another go and getting a new print in the meantime.


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OfflineEnDelfisE
Rider On The Storm
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Registered: 09/07/12
Posts: 499
Loc: Temple of Demetra
Last seen: 11 hours, 39 minutes
Re: Cultivating Panaeolus cyanescens [Re: Asura]
    #27553280 - 11/22/21 07:35 AM (2 years, 2 months ago)

I'm sooo super excited to start the journey into PanLand!

Please tell me there is some good pheno in these two plates



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OfflineRoscoepan
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Registered: 11/06/21
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Re: Cultivating Panaeolus cyanescens [Re: Asura]
    #27571167 - 12/06/21 09:39 AM (2 years, 1 month ago)

Following


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Offlineflannel
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Registered: 01/12/20
Posts: 19
Last seen: 1 month, 21 days
Re: Cultivating Panaeolus cyanescens [Re: Roscoepan]
    #27574144 - 12/08/21 10:00 PM (2 years, 1 month ago)

I need some help, I've used a whole spore syringe on agar plates trying to find a good phenotype but haven't seen one yet.  I think the appearance is fine but every sample I've isolated takes about three times longer to fill the plate than the cubes I've been dealing with.  I think I've followed the agar recipe exactly, the only difference might be that I store my inoculated plates at around 73-79 F and maybe they should be colder.  I did try to make LC with one sample I isolated but if it grew it was extremely slow.  Any advice is appreciated!


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OfflineLeftSideDriver
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Registered: 10/16/18
Posts: 62
Last seen: 1 month, 14 days
Re: Cultivating Panaeolus cyanescens [Re: flannel]
    #27606781 - 01/04/22 01:37 PM (2 years, 23 days ago)

Does anybody else find that 1600g water is too dry?  All my ingredients are bone dry and when I stop at 1600g water I cannot squeeze a single drop out of the sub.  This does not fit the watery description in the original post.  2600g feels better and I have added up to 3000g but I am definitely getting failed bags at that point. :-( Way too wet.  I am also using oats and 250g feels / looks like too little so I am upping it to 350g.  I used prepped WBS in some of my bags and even when converting from dry weight to wet weight, I feel like I needed to add more WBS on order to have enough for the LC to grab on to if you will. 

Anyone else have to tweak this recipe a little to suit their situation?

I should have at least a couple trays of Nec-D and Rainbow Eucalyptus to show soon that have come about from this recipe / tek!  Many thanks Asura!


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OfflineAsuraS
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Registered: 08/01/11
Posts: 5,047
Loc: Texas
Last seen: 10 days, 2 hours
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Re: Cultivating Panaeolus cyanescens [Re: LeftSideDriver]
    #27617491 - 01/13/22 02:08 PM (2 years, 14 days ago)

Left, just do the math with your sub and get the water content to about 63-65%. Any recipe I have posted is really just what I like and only
meant to be a starting point. I was doing everything by weight at one point because it was just easier to put it together, but
honestly once you have a feel for what you want you can eyeball the whole thing.


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Invisiblemyc_ousin_vinny
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Registered: 04/29/20
Posts: 1,415
Re: Cultivating Panaeolus cyanescens [Re: Asura]
    #27627893 - 01/22/22 02:55 AM (2 years, 5 days ago)

I noticed this thread is not very active. Is there more up to date info I need to check out or is this a good nexus for Pan Cyan tek info? Sorry to resurrect the zombie if it's too old.


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OfflineLeftSideDriver
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Registered: 10/16/18
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Last seen: 1 month, 14 days
Re: Cultivating Panaeolus cyanescens [Re: myc_ousin_vinny]
    #27632114 - 01/25/22 12:23 PM (2 years, 2 days ago)

Quote:

myc_ousin_vinny said:
I noticed this thread is not very active. Is there more up to date info I need to check out or is this a good nexus for Pan Cyan tek info? Sorry to resurrect the zombie if it's too old.




Well the official Panaeolus thread is where most of the action is:

https://www.shroomery.org/forums/showflat.php/Number/22216410/fpart/1241/vc/1

What I recommend doing is looks in the sigs of the more knowledgeable people for their grow logs and write ups and you can either choose to do a deep dive into one of their teks / methods, or you can absorb their wisdom and forge your own path.  I found success using Asura's methods here and also with JakeOncid's methods.  The JCM chamber is stupid simple and a good way to go.  You can go back and find good stuff from BlueHelix and Mary Fairchild and see how they did it too.  We're all building on and improving each others methods.  Im starting to pick and choose bits of tek from different folks to suit my needs and preferences but I have had to keep the variables to a minimum to get a couple success stories first and I still have a ways to go on my pan journey.  Right now my second flushes are all small fruit and I don't know why! Best of luck!


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OfflineTankie_J
I like having fun
I'm a teapot


Registered: 01/09/19
Posts: 537
Last seen: 10 months, 13 days
Re: Cultivating Panaeolus cyanescens [Re: LeftSideDriver]
    #27661195 - 02/16/22 11:11 AM (1 year, 11 months ago)

Left, I noticed that too and just added water till I thought it looked good.

On another note, I cut the recipe in half and put to 4T unicorn bags. That amount filled 3 bags at around 435g each wet. Just in case anyone wanted that info.


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Offlinekhanna32
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Registered: 03/02/22
Posts: 3
Last seen: 1 year, 10 months
Re: Cultivating Panaeolus cyanescens [Re: Tankie_J]
    #27680280 - 03/02/22 10:25 PM (1 year, 10 months ago)

I dont need some fuckin asshole to tell me how to grow mushrooms. Fuck you.


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Invisiblebigfootscreepyuncl
Stranger


Registered: 11/15/20
Posts: 3,717
Loc: Gamehenge
Re: Cultivating Panaeolus cyanescens [Re: khanna32]
    #27680302 - 03/02/22 10:44 PM (1 year, 10 months ago)

Quote:

khanna32 said:
I dont need some fuckin asshole to tell me how to grow mushrooms. Fuck you.





Isn't this you asking for help 3.5 hours ago? Sounds to me like you need someone to teach you how to mind your manners


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