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Splittinghairs

Registered: 02/17/25
Posts: 1,343

Re: Ask Quick Questions, Get Quick Answers

1
#29180784 - 04/07/25 02:09 AM (1 year, 7 months ago)

Will do!

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Jrey16
Magic Kabute

Registered: 12/19/23
Posts: 826
Loc: Philippines
Last seen: 1 year, 25 days

Re: Ask Quick Questions, Get Quick Answers

[Re: Nichrome]
#29180821 - 04/07/25 03:43 AM (1 year, 7 months ago)

This is my first time dealing with Pan Cyan and manure based substrates. I was going to make the substrate right now but I noticed these white dots on the cow manure. Is this mold? It was stored in the living room for a month in this plastic bag with no AC and where I live is pretty hot and humid. The white dots are only on the bottom of this 5kg bag. The top part looks ok with no white dots. Is this still okay to use?

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DERRAYLD
Constructus

Registered: 05/13/02
Posts: 15,010
Loc: South Africa
Last seen: 39 minutes, 35 seconds

Re: Ask Quick Questions, Get Quick Answers

[Re: Jrey16]

1
#29180823 - 04/07/25 03:47 AM (1 year, 7 months ago)

It shouldn't have anything growing in the manure.

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Jrey16
Magic Kabute

Registered: 12/19/23
Posts: 826
Loc: Philippines
Last seen: 1 year, 25 days

Re: Ask Quick Questions, Get Quick Answers

[Re: DERRAYLD]
#29180824 - 04/07/25 03:49 AM (1 year, 7 months ago)

Yea I just talked to the vendor. I should of stored it in a cooler place 🤦🏽‍♂️

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Jrey16
Magic Kabute

Registered: 12/19/23
Posts: 826
Loc: Philippines
Last seen: 1 year, 25 days

Re: Ask Quick Questions, Get Quick Answers

[Re: DERRAYLD]
#29180828 - 04/07/25 03:56 AM (1 year, 7 months ago)

I just ordered more manure and it won't be here until a week from now. Will the fully colonized TTBVI pan cyan jars be ok sitting on the shelf at around 25-27 degrees celcius for that long? I have no space to put them in the fridge.

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DERRAYLD
Constructus

Registered: 05/13/02
Posts: 15,010
Loc: South Africa
Last seen: 39 minutes, 35 seconds

Re: Ask Quick Questions, Get Quick Answers

[Re: Jrey16]

1
#29180831 - 04/07/25 04:05 AM (1 year, 7 months ago)

Yes that will be fine.

You can also repasteurize your dung.

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dopesauce
Doctor

Registered: 03/19/25
Posts: 175
Last seen: 7 months, 12 days

Re: Ask Quick Questions, Get Quick Answers

1
#29180866 - 04/07/25 06:36 AM (1 year, 7 months ago)

How long should I leave my no pour agar in the pc before I try to knock it up?
And is it best to just do a drop from the syringe in the middle of the plate, or drop it on the plate and then zig zag it with a loop?

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dopesauce
Doctor

Registered: 03/19/25
Posts: 175
Last seen: 7 months, 12 days

Re: Ask Quick Questions, Get Quick Answers

[Re: dopesauce]

1
#29180868 - 04/07/25 06:43 AM (1 year, 7 months ago)

Also just trying to calculate my jarhead life. How much many quarts of spawn should I be preparing for a 650g coir substrate for an 66qt tub. Anywhere from 4-7quarts?

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catnip40
Puppet∴Master

Registered: 03/09/12
Posts: 2,760
Loc: O∴O∴
Last seen: 1 year, 1 month

Re: Ask Quick Questions, Get Quick Answers

[Re: dopesauce]

1
#29180877 - 04/07/25 07:05 AM (1 year, 7 months ago)

yes most people use 4qts now but that is the range that is/was used

i leave my no pours over night until fully cooled it seems to help with condensation from temp change, but i also use a aa pc which takes forever to cool down anyway

u can take them out when the pc is depressurized, sometimes my plates are slanted from leaving them cool inside and not really knowing if they were completely straight which can be avoided by taking them out while still warm to solidify on a flat surface but not really a big issue

i would just do a single drop without spreading it around but thats preference and it doesn't really matter either way

streaking out is good for isolating genetics or colonies away from potential contaminants

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^{∴Genetics Info∴Ps. ovoideocystidiata and Native Americans∴}

_{∴The Official Woodlovers Thread∴Outdoor Mushroom Cultivation∴}

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DERRAYLD
Constructus

Registered: 05/13/02
Posts: 15,010
Loc: South Africa
Last seen: 39 minutes, 35 seconds

Re: Ask Quick Questions, Get Quick Answers

1
#29180878 - 04/07/25 07:07 AM (1 year, 7 months ago)

If you wrap your plates from the bottom up then they will always be flat, unless of course your trivet is angled.

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catnip40
Puppet∴Master

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Re: Ask Quick Questions, Get Quick Answers

4
#29180886 - 04/07/25 07:19 AM (1 year, 7 months ago)

ill show u something flat

:earth:

:earth:

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^{∴Genetics Info∴Ps. ovoideocystidiata and Native Americans∴}

_{∴The Official Woodlovers Thread∴Outdoor Mushroom Cultivation∴}

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dopesauce
Doctor

Registered: 03/19/25
Posts: 175
Last seen: 7 months, 12 days

Re: Ask Quick Questions, Get Quick Answers

2
#29180920 - 04/07/25 08:11 AM (1 year, 7 months ago)

Is it the earth?

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I am the Lorax!

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dopesauce
Doctor

Registered: 03/19/25
Posts: 175
Last seen: 7 months, 12 days

Re: Ask Quick Questions, Get Quick Answers

1
#29180976 - 04/07/25 09:14 AM (1 year, 7 months ago)

Thank you
that’s what I had gathered, just wanted to double check. My area is pretty cold, 60’s. I have some grains I started soaking a little prematurely. I’ll just get scrubbed up and take the plates out of the pc and straight into the GB, evidently SAB>GB. I’m thinking of just opening using my GB as an SaB, so as not to make drafts… I cleaned it out before I started to PC, and Lysoled the crap out of it, and out of the room.

Fingers crossed it’s good to go. Its my 1st, I would love to not F it up.

Just shake the sport syringe up, and get it red hot, cool it with a drop, then I should only need the smallest of drops in each plate.

I’m doing pastywhytes ez lids. Should I reuse the foil? Or new foil, or no foil after I knock them up?

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I am the Lorax!

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catnip40
Puppet∴Master

Registered: 03/09/12
Posts: 2,760
Loc: O∴O∴
Last seen: 1 year, 1 month

Re: Ask Quick Questions, Get Quick Answers

[Re: dopesauce]

2
#29181013 - 04/07/25 09:45 AM (1 year, 7 months ago)

Yes ditch the gloves sab are preferred now anyway

U don't need foil after inoc

U can get a single drop by squeeze the side of the syringe rather than using the plunger

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^{∴Genetics Info∴Ps. ovoideocystidiata and Native Americans∴}

_{∴The Official Woodlovers Thread∴Outdoor Mushroom Cultivation∴}

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Mr Piggy
Swine Flew

Registered: 09/29/11
Posts: 16,768
Loc: bioilliminicent©️

Re: Ask Quick Questions, Get Quick Answers *DELETED*

[Re: EndlessB]
#29181079 - 04/07/25 10:53 AM (1 year, 7 months ago)

Post deleted by Mr Piggy

Reason for deletion: .

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🅃🄴🄰🄼 🄸🄷🄾🄿

New to cultivation or the shroomery? Observe the hitchhiker's guide
The Hitchhiker's Guide to the Shroomery

Smellyhobbit said:
"Wiping a needle with isopropyl after flame sterilization is like shitting after you wipe your ass."

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dinosaurs
Permanently Banned

Registered: 03/10/25
Posts: 2,121

Re: Ask Quick Questions, Get Quick Answers

1
#29181083 - 04/07/25 10:56 AM (1 year, 7 months ago)

Mr Piggy said:

EndlessB said:

LC from a vendor. I tested a little bit on agar first to see if it was clean

jeez I normally just taste it. this guys one of those scientists over here.

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Mr Piggy
Swine Flew

Registered: 09/29/11
Posts: 16,768
Loc: bioilliminicent©️

Re: Ask Quick Questions, Get Quick Answers

3
#29181087 - 04/07/25 10:58 AM (1 year, 7 months ago)

EndlessB said:

For my first time, I thought it was easier to be sterile with that method than trying to go agar-to-grain. I knew I was taking a risk though.

It is my experience that nothing is cleaner than agar to grain. You can see exactly how clean your culture is on that plate, but LC is a mystery... especially LC you didn't make yourself.

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🅃🄴🄰🄼 🄸🄷🄾🄿

New to cultivation or the shroomery? Observe the hitchhiker's guide
The Hitchhiker's Guide to the Shroomery

Smellyhobbit said:
"Wiping a needle with isopropyl after flame sterilization is like shitting after you wipe your ass."

Extras:

Mr Piggy
Swine Flew

Registered: 09/29/11
Posts: 16,768
Loc: bioilliminicent©️

Re: Ask Quick Questions, Get Quick Answers

[Re: SkanskKorv]

1
#29181095 - 04/07/25 11:02 AM (1 year, 7 months ago)

SkanskKorv said:

just after ordering a pc.

Okay just caught this. Yeah, nothing you have made yet is clean. You didn't "sterilize" anything without a PC.

Folks, DO NOT mix and match methods, especially when you're not even using key tools you need.

Your spore LC is probably a soup of contaminants, and at this point you do not have the skill set to tell otherwise.

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🅃🄴🄰🄼 🄸🄷🄾🄿

New to cultivation or the shroomery? Observe the hitchhiker's guide
The Hitchhiker's Guide to the Shroomery

Smellyhobbit said:
"Wiping a needle with isopropyl after flame sterilization is like shitting after you wipe your ass."

Extras:

Meanwhile
Non-Stop Venting

Registered: 07/26/24
Posts: 158
Loc: USA

Last seen: 6 months, 18 days

Re: Ask Quick Questions, Get Quick Answers

[Re: Mr Piggy]
#29181271 - 04/07/25 02:04 PM (1 year, 7 months ago)

Has there ever been any correlations made for different kinds of seeds to potency, and to go along with that letting a colonized jar of said seed sit for X amount of time after full colonization to increase potency ? Just curious, I saw a post a while back with someone testing it. Thought I had it added to my favorites but alas I lost it :/

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eCamp78
Stranger

Registered: 03/29/24
Posts: 34
Last seen: 1 year, 1 month

Re: Ask Quick Questions, Get Quick Answers

[Re: Nichrome]
#29181274 - 04/07/25 02:08 PM (1 year, 7 months ago)

This little guy was deteriorating and ended up knocking it over with my hygrometer. Had some white fuzz on the stem as well. Contaminated or safe?

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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation

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