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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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Re: Ask Quick Questions, Get Quick Answers [Re: Whoozer]
    #29177793 -

Send it :grin:

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Re: Ask Quick Questions, Get Quick Answers [Re: ragamuffin]
    #29177805 -

I have some oysters I put to grain today. My question is on masters mix. Do I pasteurize it, or just run the bags through to PC and sterilize?


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Hitchhikers guide to the Shroomery

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Re: Ask Quick Questions, Get Quick Answers [Re: Breadly710]
    #29177821 -

Agar contam troubleshooting

What info do I need to give you to get input on what's causing the yellow color and lack of mycelium growth?



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Re: Ask Quick Questions, Get Quick Answers [Re: Breadly710] * 1
    #29177829 -

Breadly710 said:
I have some oysters I put to grain today. My question is on masters mix. Do I pasteurize it, or just run the bags through to PC and sterilize?


I sterilize my master’s mix bags.


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Method for Small Scale Casing Pasteurization

How much really fits in a 00 capsule?

Micro and Macro Mushroom Photography

Can you freeze mycelium?


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Re: Ask Quick Questions, Get Quick Answers [Re: RoscoeReturns] * 1
    #29177847 -

That was what I thought, but wanted to make sure. Thanks Roscoe!!!


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Hitchhikers guide to the Shroomery

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Re: Ask Quick Questions, Get Quick Answers [Re: maplepine]
    #29177849 -

maplepine said:
Agar contam troubleshooting

What info do I need to give you to get input on what's causing the yellow color and lack of mycelium growth?





That looks like a culture of nothing but slime mold to me.


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The Shroomery Manual
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GC’s Ayahuasca Recipe
Free Therapy

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Re: Ask Quick Questions, Get Quick Answers [Re: RoscoeReturns] * 1
    #29177850 -

RoscoeReturns said:
Breadly710 said:
I have some oysters I put to grain today. My question is on masters mix. Do I pasteurize it, or just run the bags through to PC and sterilize?


I sterilize my master’s mix bags.


:whathesaid::heart::heart:
:manofapproval:


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:ancientaliens:

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Re: Ask Quick Questions, Get Quick Answers [Re: astra]
    #29177860 -

It depends on the supplements really. I’ve used alfalfa pellets in my “masters mix” instead of soy hulls, and they come heat treated. Anything that isn’t pelletized gets sterilized


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Thank your postal workers when you see them. None of this would be possible without them.

Necessity is the mother of invention

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Re: Ask Quick Questions, Get Quick Answers [Re: EniQma]
    #29177908 -

Same ratio?

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Re: Ask Quick Questions, Get Quick Answers [Re: maplepine]
    #29177911 -

maplepine said:
Agar contam troubleshooting

What info do I need to give you to get input on what's causing the yellow color and lack of mycelium growth?






Info needed...
Your process.

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Re: Ask Quick Questions, Get Quick Answers [Re: LewDoja] * 1
    #29177957 -

Never heard of it.  What's BTI?
I wish I had though cuz those damn yellow sticky strips get on my nerves.

I've got the stupid little flies in the drains of bathroom sink so I definitely want to take some preventative measures just in case they decide to migrate on over into the grow space.  which is in the same bathroom.


I just got my grow room all filled up with projects so this is like a five-star Resort for those little buggers if they notice their neighbors with it.



I work in the pool service industry rn. I've got a bunch of DE (diatomaceous earth) 

Just curious is that stuff good for anything In this hobby? I know it is used in gardening but don't know what for.


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:hippie::mushroom2:Take it easy. if it's easy. take it twice:mushroom2::mushroom2:
:sporedrop:The Hitchhiker's Guide to the Shroomery:sporedrop:

**A LINK TO EVERTHING**


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Re: Ask Quick Questions, Get Quick Answers [Re: callmehippie] * 5
    #29178025 -

Everybody knows it . I Just gonna say it again.


@lewdoja. 

:yourock:


Always going outta your way to ensure everyone is happy. i def strive to be able to fill your shoes around here some day.

IOU a friggn bunch man.(for now you get my praise  and a badass trade rating):wink:

Honestly..
Everyone around here is amazing.


  Anyways I'm in a good mood.  Just wanted to share  with my buddies. 

Stay groovy


--------------------
:hippie::mushroom2:Take it easy. if it's easy. take it twice:mushroom2::mushroom2:
:sporedrop:The Hitchhiker's Guide to the Shroomery:sporedrop:

**A LINK TO EVERTHING**


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Re: Ask Quick Questions, Get Quick Answers [Re: callmehippie]
    #29178027 -

BTI is BACILLUS THURINGIENSIS ISRAELENSIS, you get various Bacillus bacteria that are used to combat certain insects.

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Re: Ask Quick Questions, Get Quick Answers [Re: LewDoja]
    #29178047 -

LewDoja said:
Info needed...
Your process.


LOL OK!

Agar: oatmeal soak water, a bit of corn syrup, stick agar (I've gotten malt extract since then and will change the recipe going forward)

PC'd agar in InstantPot 45 minutes, poured into condiment cups in SAB. (will use glass going forward)
Let sit for a few days
Heated homemade steel wire spore spreader in alcohol lamp til red hot, cooled in separate agar pot inside SAB
Swabbed homemade spore print, made first spread in container
Re-heated spreader, cooled, made 2nd pattern in container

For the container pictured I think I only did 1 pattern.

I will make changes to my agar recipe and containers (plastic -> glass) but the data point I'm thinking about now is that the agar pot that I used for cooling the spreader has no contam, whereas ALL of the inoculated containers do. I guess that points to the spore print?? Or the water I used to spread. Is this reasoning correct?

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Re: Ask Quick Questions, Get Quick Answers [Re: maplepine] * 1
    #29178050 -

That's bacterial so either you aren't sterilizing your loop fully or your water is not sterile.

Those are some options, generally speaking satellite molds are more attributed to our own technique issues.

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Re: Ask Quick Questions, Get Quick Answers [Re: maplepine] * 1
    #29178075 -

You mention water used to spread...but nothing about preparing it. Did it go in the IP along with the agar media?

In any case, you can skip the water and remove a possible contamination vector. Just cool the loop in the receiving plate (no need for a separate cooling dish either), pick up some spores with the  agar-wet loop, and streak on the plate. A sterile swab works well for this too - it's more wasteful but also a little less fussy.

Even if the print is dirty, avoiding adding water may give any mycelium that germinates a better chance of outrunning present bacteria long enough for you to take a clean transfer.


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LAGM 2026 grow log | My library/trade list

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Re: Ask Quick Questions, Get Quick Answers [Re: Phaethon] * 2
    #29178232 -

this goes out to roscoe, nich, and the other medicinal and gourmet people:

i want to grow chaga.  i forage it from my place in maine but i dont feel good about that.  really.

ive never heard of anyone successfully growing but id like to try.  if anyone has any ideas, dm me.  i have an infinite supply of birch wood/bark.


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The Cultivation Confessional Booth - The OFFICIAL Cultivation Off Topic Discussion

✸-✸-✸

The Hitchhiker's Guide to the Shroomery

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Re: Ask Quick Questions, Get Quick Answers [Re: BeefExtreme] * 1
    #29178236 -

7suns is currently growing chaga in bags;

https://www.shroomery.org/forums/showflat.php/Number/29114177/fpart/1/vc/1

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Re: Ask Quick Questions, Get Quick Answers [Re: Phaethon] * 1
    #29178267 -

DERRAYLD said:
That's bacterial so either you aren't sterilizing your loop fully or your water is not sterile.

Those are some options, generally speaking satellite molds are more attributed to our own technique issues.


Phaethon said:
You mention water used to spread...but nothing about preparing it. Did it go in the IP along with the agar media?

In any case, you can skip the water and remove a possible contamination vector. Just cool the loop in the receiving plate (no need for a separate cooling dish either), pick up some spores with the  agar-wet loop, and streak on the plate. A sterile swab works well for this too - it's more wasteful but also a little less fussy.

Even if the print is dirty, avoiding adding water may give any mycelium that germinates a better chance of outrunning present bacteria long enough for you to take a clean transfer.


Thanks so much, this makes a lot of sense. I boiled the syringe in water and sucked up some of the boil water, as I saw in a video. However, I also stored it in a ziplock in the fridge, so even though I heated the needle to glowing, there's enough doubt in the rest of the process to point the finger.

I'll look for sterile swabs. I want to avoid ordering things like lab equipment but that should be safe.

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Re: Ask Quick Questions, Get Quick Answers [Re: maplepine] * 1
    #29178276 -

You can sterilize cotton swabs by wrapping normal ear buds in tin foil and pcing them in a sealed jar.

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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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