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ragamuffin
Stranger

Registered: 12/19/22
Posts: 3
Last seen: 1 year, 7 months

Re: Ask Quick Questions, Get Quick Answers

[Re: Whoozer]
#29177793 - 04/03/25 08:42 PM (1 year, 7 months ago)

Send it

:grin:

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Breadly710
Baker

Registered: 02/19/24
Posts: 582
Last seen: 14 days, 17 hours

Re: Ask Quick Questions, Get Quick Answers

[Re: ragamuffin]
#29177805 - 04/03/25 08:50 PM (1 year, 7 months ago)

I have some oysters I put to grain today. My question is on masters mix. Do I pasteurize it, or just run the bags through to PC and sterilize?

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maplepine
Stranger

Registered: 01/31/25
Posts: 70
Last seen: 10 months, 28 days

Re: Ask Quick Questions, Get Quick Answers

[Re: Breadly710]
#29177821 - 04/03/25 09:03 PM (1 year, 7 months ago)

Agar contam troubleshooting

What info do I need to give you to get input on what's causing the yellow color and lack of mycelium growth?

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RoscoeReturns
We’ve outstayed our welcome

Registered: 02/13/18
Posts: 3,129
Loc: State of Confusion
Last seen: 20 hours, 42 minutes

Re: Ask Quick Questions, Get Quick Answers

[Re: Breadly710]

1
#29177829 - 04/03/25 09:06 PM (1 year, 7 months ago)

Breadly710 said:

I have some oysters I put to grain today. My question is on masters mix. Do I pasteurize it, or just run the bags through to PC and sterilize?

I sterilize my master’s mix bags.

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Method for Small Scale Casing Pasteurization

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Micro and Macro Mushroom Photography

Can you freeze mycelium?

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Breadly710
Baker

Registered: 02/19/24
Posts: 582
Last seen: 14 days, 17 hours

Re: Ask Quick Questions, Get Quick Answers

[Re: RoscoeReturns]

1
#29177847 - 04/03/25 09:19 PM (1 year, 7 months ago)

That was what I thought, but wanted to make sure. Thanks Roscoe!!!

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Hitchhikers guide to the Shroomery

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GenesisCorrupted
Cowboy, Cultivator, Dude

Registered: 08/01/23
Posts: 33,864
Loc: PNW

Re: Ask Quick Questions, Get Quick Answers

[Re: maplepine]
#29177849 - 04/03/25 09:21 PM (1 year, 7 months ago)

maplepine said:

Agar contam troubleshooting

What info do I need to give you to get input on what's causing the yellow color and lack of mycelium growth?

That looks like a culture of nothing but slime mold to me.

--------------------

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astra
Mycelia-Of-House-Fungi

Registered: 12/24/21
Posts: 1,118
Loc: Japan

Re: Ask Quick Questions, Get Quick Answers

[Re: RoscoeReturns]

1
#29177850 - 04/03/25 09:21 PM (1 year, 7 months ago)

RoscoeReturns said:

Breadly710 said:

I have some oysters I put to grain today. My question is on masters mix. Do I pasteurize it, or just run the bags through to PC and sterilize?

I sterilize my master’s mix bags.

:whathesaid:

:heart:

:heart:

:manofapproval:

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:ancientaliens:

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EniQma
PȘÝČĦËĎËŁĪČ

Registered: 11/28/23
Posts: 2,456

Re: Ask Quick Questions, Get Quick Answers

[Re: astra]
#29177860 - 04/03/25 09:31 PM (1 year, 7 months ago)

It depends on the supplements really. I’ve used alfalfa pellets in my “masters mix” instead of soy hulls, and they come heat treated. Anything that isn’t pelletized gets sterilized

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LewDoja
mad $cientist, ganjacologist

Registered: 09/18/09
Posts: 4,849
Loc: A fictional tale, in fictional...
Last seen: 6 months, 9 hours

Re: Ask Quick Questions, Get Quick Answers

[Re: EniQma]
#29177908 - 04/03/25 10:11 PM (1 year, 7 months ago)

Same ratio?

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LewDoja
mad $cientist, ganjacologist

Registered: 09/18/09
Posts: 4,849
Loc: A fictional tale, in fictional...
Last seen: 6 months, 9 hours

Re: Ask Quick Questions, Get Quick Answers

[Re: maplepine]
#29177911 - 04/03/25 10:14 PM (1 year, 7 months ago)

maplepine said:

Agar contam troubleshooting

What info do I need to give you to get input on what's causing the yellow color and lack of mycelium growth?

Info needed...
Your process.

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callmehippie
☮️🍄Hallucinogenic Hippie🍄☮️

Registered: 05/28/24
Posts: 3,509
Loc: Over the hills and far away

Last seen: 5 hours, 15 seconds

Re: Ask Quick Questions, Get Quick Answers

[Re: LewDoja]

1
#29177957 - 04/03/25 11:00 PM (1 year, 7 months ago)

Never heard of it. What's BTI?
I wish I had though cuz those damn yellow sticky strips get on my nerves.

I've got the stupid little flies in the drains of bathroom sink so I definitely want to take some preventative measures just in case they decide to migrate on over into the grow space. which is in the same bathroom.

I just got my grow room all filled up with projects so this is like a five-star Resort for those little buggers if they notice their neighbors with it.

I work in the pool service industry rn. I've got a bunch of DE (diatomaceous earth)

Just curious is that stuff good for anything In this hobby? I know it is used in gardening but don't know what for.

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:hippie:

:hippie:

:mushroom2:

Take it easy. if it's easy. take it twice :mushroom2:

:mushroom2:

:mushroom2:

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callmehippie
☮️🍄Hallucinogenic Hippie🍄☮️

Registered: 05/28/24
Posts: 3,509
Loc: Over the hills and far away

Last seen: 5 hours, 15 seconds

Re: Ask Quick Questions, Get Quick Answers

[Re: callmehippie]

5
#29178025 - 04/04/25 12:15 AM (1 year, 7 months ago)

Everybody knows it . I Just gonna say it again.

@lewdoja.

:yourock:

:yourock:

Always going outta your way to ensure everyone is happy. i def strive to be able to fill your shoes around here some day.

IOU a friggn bunch man.(for now you get my praise and a badass trade rating) :wink:

:wink:

Honestly..
Everyone around here is amazing.

Anyways I'm in a good mood. Just wanted to share with my buddies.

Stay groovy

--------------------
:hippie:

:hippie:

:mushroom2:

Take it easy. if it's easy. take it twice :mushroom2:

:mushroom2:

:mushroom2:

:sporedrop:

The Hitchhiker's Guide to the Shroomery

:sporedrop:

**A LINK TO EVERTHING**

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DERRAYLD
Constructus

Registered: 05/13/02
Posts: 15,024
Loc: South Africa
Last seen: 6 minutes, 59 seconds

Re: Ask Quick Questions, Get Quick Answers

[Re: callmehippie]
#29178027 - 04/04/25 12:18 AM (1 year, 7 months ago)

BTI is BACILLUS THURINGIENSIS ISRAELENSIS, you get various Bacillus bacteria that are used to combat certain insects.

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maplepine
Stranger

Registered: 01/31/25
Posts: 70
Last seen: 10 months, 28 days

Re: Ask Quick Questions, Get Quick Answers

[Re: LewDoja]
#29178047 - 04/04/25 01:00 AM (1 year, 7 months ago)

LewDoja said:

Info needed...
Your process.

LOL OK!

Agar: oatmeal soak water, a bit of corn syrup, stick agar (I've gotten malt extract since then and will change the recipe going forward)

PC'd agar in InstantPot 45 minutes, poured into condiment cups in SAB. (will use glass going forward)
Let sit for a few days
Heated homemade steel wire spore spreader in alcohol lamp til red hot, cooled in separate agar pot inside SAB
Swabbed homemade spore print, made first spread in container
Re-heated spreader, cooled, made 2nd pattern in container

For the container pictured I think I only did 1 pattern.

I will make changes to my agar recipe and containers (plastic -> glass) but the data point I'm thinking about now is that the agar pot that I used for cooling the spreader has no contam, whereas ALL of the inoculated containers do. I guess that points to the spore print?? Or the water I used to spread. Is this reasoning correct?

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DERRAYLD
Constructus

Registered: 05/13/02
Posts: 15,024
Loc: South Africa
Last seen: 6 minutes, 59 seconds

Re: Ask Quick Questions, Get Quick Answers

[Re: maplepine]

1
#29178050 - 04/04/25 01:04 AM (1 year, 7 months ago)

That's bacterial so either you aren't sterilizing your loop fully or your water is not sterile.

Those are some options, generally speaking satellite molds are more attributed to our own technique issues.

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Phaethon
Hobbyist

Registered: 07/04/22
Posts: 1,270
Loc: Colorado

Re: Ask Quick Questions, Get Quick Answers

[Re: maplepine]

1
#29178075 - 04/04/25 02:17 AM (1 year, 7 months ago)

You mention water used to spread...but nothing about preparing it. Did it go in the IP along with the agar media?

In any case, you can skip the water and remove a possible contamination vector. Just cool the loop in the receiving plate (no need for a separate cooling dish either), pick up some spores with the agar-wet loop, and streak on the plate. A sterile swab works well for this too - it's more wasteful but also a little less fussy.

Even if the print is dirty, avoiding adding water may give any mycelium that germinates a better chance of outrunning present bacteria long enough for you to take a clean transfer.

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BeefExtreme
Meatkeeper

Registered: 11/03/11
Posts: 14,592
Loc:

Last seen: 2 hours, 37 minutes

Re: Ask Quick Questions, Get Quick Answers

2
#29178232 - 04/04/25 08:50 AM (1 year, 7 months ago)

this goes out to roscoe, nich, and the other medicinal and gourmet people:

i want to grow chaga. i forage it from my place in maine but i dont feel good about that. really.

ive never heard of anyone successfully growing but id like to try. if anyone has any ideas, dm me. i have an infinite supply of birch wood/bark.

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The Cultivation Confessional Booth - The OFFICIAL Cultivation Off Topic Discussion
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DERRAYLD
Constructus

Registered: 05/13/02
Posts: 15,024
Loc: South Africa
Last seen: 6 minutes, 59 seconds

Re: Ask Quick Questions, Get Quick Answers

[Re: BeefExtreme]

1
#29178236 - 04/04/25 08:54 AM (1 year, 7 months ago)

7suns is currently growing chaga in bags;

https://www.shroomery.org/forums/showflat.php/Number/29114177/fpart/1/vc/1

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maplepine
Stranger

Registered: 01/31/25
Posts: 70
Last seen: 10 months, 28 days

Re: Ask Quick Questions, Get Quick Answers

1
#29178267 - 04/04/25 09:29 AM (1 year, 7 months ago)

DERRAYLD said:

That's bacterial so either you aren't sterilizing your loop fully or your water is not sterile.

Those are some options, generally speaking satellite molds are more attributed to our own technique issues.

Phaethon said:

You mention water used to spread...but nothing about preparing it. Did it go in the IP along with the agar media?

In any case, you can skip the water and remove a possible contamination vector. Just cool the loop in the receiving plate (no need for a separate cooling dish either), pick up some spores with the agar-wet loop, and streak on the plate. A sterile swab works well for this too - it's more wasteful but also a little less fussy.

Even if the print is dirty, avoiding adding water may give any mycelium that germinates a better chance of outrunning present bacteria long enough for you to take a clean transfer.

Thanks so much, this makes a lot of sense. I boiled the syringe in water and sucked up some of the boil water, as I saw in a video. However, I also stored it in a ziplock in the fridge, so even though I heated the needle to glowing, there's enough doubt in the rest of the process to point the finger.

I'll look for sterile swabs. I want to avoid ordering things like lab equipment but that should be safe.

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DERRAYLD
Constructus

Registered: 05/13/02
Posts: 15,024
Loc: South Africa
Last seen: 6 minutes, 59 seconds

Re: Ask Quick Questions, Get Quick Answers

[Re: maplepine]

1
#29178276 - 04/04/25 09:35 AM (1 year, 7 months ago)

You can sterilize cotton swabs by wrapping normal ear buds in tin foil and pcing them in a sealed jar.

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