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Re: Official Copelandia/Panaeolus Thread [Re: tree frog]
    #29141113 -

Interesting :strokebeard:

I might try that with the cow manure compost I have in one mini mono if, and when I have som clean spawn with bisporus.

How did you pasteurize and what went wrong?


--------------------
“All Fungi are edible.

Some fungi are only edible once.”
― Terry Pratchett


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Re: Official Copelandia/Panaeolus Thread [Re: Kloakk] * 3
    #29141114 -

Kloakk said:
RoscoeReturns said:
I’ve had no problem running bisporus on wheat.



This is great news, do you mind sharing your wheat prep?




My wheat prep is pretty standard, goes something like this:

Put wheat in big pot.

Cover with cold water, stir with my hand, dump all the water.

Repeat the rinse a second time.

Add water to pot double the height of the wheat.

Place on stove over medium high heat and bring to simmer, not a boil, reduce heat to maintain simmer.

Simmer until done, stirring occasionally, about 40 minutes. I start checking around the 35 minute mark. When you split a grain it should be translucent all the way through, no hard white endosperm.

Drain and place on screen or towels and allow to air dry. I want the outside to be completely dry and not sticking together or to my hand.

Load jars and PC.

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Re: Official Copelandia/Panaeolus Thread [Re: RoscoeReturns] * 3
    #29141131 -

Standard or not, it's helpful to me, thank you :hatsoff:

The reason I asked is because you wrote that bisporus is doing fine on your wheat. After Tree Frog told me she and others where having problem with bisporus on certain grains it sent me down a rabbit hole in search of why this is.

One of the things I found while digging was the fact that fungicide treatment are quite different on some crops, like popcorn, usually corn is a lot more resistant to fungal infection, and don't use as much or as many fungicides as other crops do.

So it got me thinking that maybe bisporus don't have alot of fungicide in it's natural habitat and if people where doing no prep jars without even rinsing the grains it's a possibility it effects the growth, just a thought I had.

So yeah, standard or not it helps me, I use 2 drops of dish soap on my first rinse, but you don't so that is one thing I can check of my list, I'll still do it, but not be to worried about how I do it and just go with my usual prep flow :rockon:


--------------------
“All Fungi are edible.

Some fungi are only edible once.”
― Terry Pratchett


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Re: Official Copelandia/Panaeolus Thread [Re: tree frog] * 2
    #29141134 -

tree frog said:
https://www.shroomery.org/forums/showflat.php/Number/25546266

Here's a grow log where someone used black cow straight out of the bag. I've been thinking this stuff was already heat treated, and I think I just messed a batch up by trying to he treat it and lost a few grows because of it.

I have some projects on plates, after they colonize some jars I will give it a try as I already have a bag and a half here.




I don't remember the exact brand I bought, but I have some composted manure that I bought last year that was kept in a sealed Rubbermaid tote. I just opened it to use for AIO, and it was overly damp but zero mold. It smelled neutral, which is exactly how it smelled the day I put it in the tote.

You would think a nutrient medium being opened, dumped into a non-sterile tote, and then sealed in my warm basement would be an ideal way to grow mold, but it didn't.


--------------------
I've got skills, they don't know where to use me
I'm like the best dressed guy at the nude beach
Nice to meet you!

Edited by PuffM4gicDr4gon (02/28/25 06:40 AM)

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Re: Official Copelandia/Panaeolus Thread [Re: Baba Yaga] * 2
    #29141143 -

Baba Yaga said:
So what amount of fresh Burringbar have you guys tried? I have a window to journey tomorrow. The wife and kid went on vacation for a few days, just a couple of cats around.

Torn between Bbar fresh or some dry subtrops which I haven't tried yet either :dancingshroom:



Maaan, I actually haven’t even tried Bbar fresh but .75 dry sent me to my room to watch the light show, can’t wait to hear how it went!

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Re: Official Copelandia/Panaeolus Thread [Re: 3.A.M] * 5
    #29141308 -

A slow moving ttbvi.  It's also got trich due to a pasteurization failure.  So maybe that's why it was slow.



Also trich, same substrate.  Pan. Chlorocystis.




I think I have two or three pins.  So hopefully I get a couple prints, even if I don't get enough to do a proper sample out of this run.


--------------------
And if I only could
I'd make a deal with God
And I'd get him to swap our places
I'd be running up that road
Be running up that hill
With no problems

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Re: Official Copelandia/Panaeolus Thread [Re: tree frog] * 1
    #29141364 -

Do you guys change your water in your jcm or do anything special if you end up with green mold in there? I have a couple pf tek cakes that I'd like to use as a test run while I wait for my aio jars to colonize, but tubs of that strain have been throwing green. I'd love to get a clean clone but I'm not sure if it's worth the trouble.


--------------------
I've got skills, they don't know where to use me
I'm like the best dressed guy at the nude beach
Nice to meet you!

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Re: Official Copelandia/Panaeolus Thread [Re: tree frog] * 1
    #29141670 -

tree frog said:
https://www.shroomery.org/forums/showflat.php/Number/25546266

Here's a grow log where someone used black cow straight out of the bag. I've been thinking this stuff was already heat treated, and I think I just messed a batch up by trying to he treat it and lost a few grows because of it.

I have some projects on plates, after they colonize some jars I will give it a try as I already have a bag and a half here.




99.99 uses black kow straight from the bag for all of their mexicana grows.

Just touching on the contamination you were experiencing. Was that with grass cased substrates by any chance? I'm asking because I spawned a few different semilanceata substrates lately and am contemplating whether to sow grass seed or not. I am unsure cause the seeds I have are 3+ years old now and I am thinking that they will have lost quite a bit of viability and non germinating seeds would be a contam vector. They still work great and I do use them on the outdoor subs but it's a fact that the older they get the more will have lost the spark to grow.

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Re: Official Copelandia/Panaeolus Thread [Re: Baba Yaga] * 9
    #29141672 -

The Burringbar I just grew were slow moving as well, took 2 weeks from pins to maturation and they have small caps. Might be because I used 6 month old cultures I kept in the cupboard and that I didn't use my usual pan sub recipe but used the surplus I made for the semilanceate, less compost and more grass clippings or maybe I am just fishing for reasons to let my cultivators pride off the hook :lol:


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Re: Official Copelandia/Panaeolus Thread [Re: astra] * 1
    #29141874 -

astra said:
HoodedBingling said:
I appreciate your rambling! The cloud watching is now on my bucket list. The auditory stuff I got personally was weird. On the comeup I was getting a whole bunch of typical repetitive robotic noises like sirens and a lot of other stuff I cannot pin point now. The setting i did them in was very weird in itself anyhow so I look forward to do over 2g (1,6 last time) somewhere nice. Even outside which for now in scandinavia was a no no.
My cyans got me seeing yellow greenish. I have the original print of the ttbvi so it would be fun to share both the original culture and the one I manipulated to see if others sense the huge difference as well.
Shutting up before self banning again 😝🤐



TTBVI will do this to me in VERY high doses, like straight out android voices-like auditory hallucinations. Lots of electric blue and radiant silver hues coming in by the millions per second. Unlike other pan varieties, you gotta tune them down or else they become unbearable lol
:rush:

They're extremely powerful little guys. The less the better :heart::heart:


ps,
Absolutely NOT a typical dose, i promise :blush:




I cannot imagine more 1g of my TTBVI.  0.68g was the bomb.  The 1g was awesome, but can't imagine going higher.  My body was gone.  I could not tell if I was breathing.  Tried to die during that experience, as in give my life for the world.  Tried to let myself be eaten my others....not that there were distinct entities.  Needless to say, I did not die.  I was fused in a symbiotic relationship with the mushroom and asserted myself as an entity................crazy, crazy stuff.

Going to do 500mg next time.  I think less is more.  I think you are right.


--------------------
I may not have stayed at a Holiday Inn Express, but I have taken 20 grams of fresh subtropicalis :smile:

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Re: Official Copelandia/Panaeolus Thread [Re: Hardhead] * 1
    #29141918 -

Is cap diameter more dependent on genetics or nutrition? I can’t seem to get any of my grows to match wild caps, and even then there doesn’t seem to be any real consistency. Bigger one time, smaller the next, just all over the place.

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Re: Official Copelandia/Panaeolus Thread [Re: Hardhead]
    #29141924 -

hardhead43 said:
astra said:
HoodedBingling said:
I appreciate your rambling! The cloud watching is now on my bucket list. The auditory stuff I got personally was weird. On the comeup I was getting a whole bunch of typical repetitive robotic noises like sirens and a lot of other stuff I cannot pin point now. The setting i did them in was very weird in itself anyhow so I look forward to do over 2g (1,6 last time) somewhere nice. Even outside which for now in scandinavia was a no no.
My cyans got me seeing yellow greenish. I have the original print of the ttbvi so it would be fun to share both the original culture and the one I manipulated to see if others sense the huge difference as well.
Shutting up before self banning again 😝🤐



TTBVI will do this to me in VERY high doses, like straight out android voices-like auditory hallucinations. Lots of electric blue and radiant silver hues coming in by the millions per second. Unlike other pan varieties, you gotta tune them down or else they become unbearable lol
:rush:

They're extremely powerful little guys. The less the better :heart::heart:


ps,
Absolutely NOT a typical dose, i promise :blush:




I cannot imagine more 1g of my TTBVI.  0.68g was the bomb.  The 1g was awesome, but can't imagine going higher.  My body was gone.  I could not tell if I was breathing.  Tried to die during that experience, as in give my life for the world.  Tried to let myself be eaten my others....not that there were distinct entities.  Needless to say, I did not die.  I was fused in a symbiotic relationship with the mushroom and asserted myself as an entity................crazy, crazy stuff.

Going to do 500mg next time.  I think less is more.  I think you are right.



Made a tea with lemon and pomegranate out of twelve grams of fresh from my most recent grow and the world just sort of stopped existing for awhile. An intriguing experience, but not one I’m eager to repeat any time soon.

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Re: Official Copelandia/Panaeolus Thread [Re: Baba Yaga] * 1
    #29141958 -

Baba Yaga said:
tree frog said:
https://www.shroomery.org/forums/showflat.php/Number/25546266

Here's a grow log where someone used black cow straight out of the bag. I've been thinking this stuff was already heat treated, and I think I just messed a batch up by trying to he treat it and lost a few grows because of it.

I have some projects on plates, after they colonize some jars I will give it a try as I already have a bag and a half here.



99.99 uses black kow straight from the bag for all of their mexicana grows.

Just touching on the contamination you were experiencing. Was that with grass cased substrates by any chance? I'm asking because I spawned a few different semilanceata substrates lately and am contemplating whether to sow grass seed or not. I am unsure cause the seeds I have are 3+ years old now and I am thinking that they will have lost quite a bit of viability and non germinating seeds would be a contam vector. They still work great and I do use them on the outdoor subs but it's a fact that the older they get the more will have lost the spark to grow.



Nah, I have clear trays and I keep an eye on them. The trich wasn't even on the surface yet when I tossed this one.  Spawned with the same batch of over pasteurized black kow the chlorocystis was.  And I had some of that leftover on my shelf, the black cow that I had pasteurized. It had mold in it and smelled like ammonia.



Here's the trich in this second picture.  Floated the sub to harvest.




So it's both the grass and not the grass. And I've been using grass for close to a year now.  If seeds don't germinate, the mycelium just eats them.

Here's some actual nats not ochra, same grass but the black kow was sterilized.  I tried to put some ungerminated grass in the center of the picture, so you can see how the mycelium even of this slower species ate the seeds.



Edit:  grass germinates pretty quick, you could test it and if it doesn't get a good germination rate, yeah it might be better not to use. If you use too much it definitely invites competition in the casing because of the microclimate.  In which case I usually have to dial the misting way back and up the fresh air exchange to compensate. The trays usually recover though.


--------------------
And if I only could
I'd make a deal with God
And I'd get him to swap our places
I'd be running up that road
Be running up that hill
With no problems

Edited by tree frog (03/01/25 03:12 AM)

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Re: Official Copelandia/Panaeolus Thread [Re: tree frog] * 3
    #29142363 -

Consolidated photos plus commentary on growing with plants over the last year or so (mostly grass atm).


--------------------
And if I only could
I'd make a deal with God
And I'd get him to swap our places
I'd be running up that road
Be running up that hill
With no problems

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Re: Official Copelandia/Panaeolus Thread [Re: tree frog] * 6
    #29142675 -

Good time, friends, who grew pan RDU?
Of all the fungi, 90% of the spores are dark gray, the rest are red.
Three agar transitions, then liquid culture and grain.
Are there any possible changes in genetics?

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Re: Official Copelandia/Panaeolus Thread [Re: RoscoeReturns] * 7
    #29143380 -

Apologies for the self-ban, needed a few days to clear my head from awful thoughts. Thank you for the beautiful, caring messages and support, fam :heart::heart:

:nicesmile::loveheart:

ps,
Tree Frog I love you very much Sis :hug:


--------------------
:ancientaliens:

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Re: Official Copelandia/Panaeolus Thread [Re: PuffM4gicDr4gon]
    #29143385 -

PuffM4gicDr4gon said:
Do you guys change your water in your jcm or do anything special if you end up with green mold in there? I have a couple pf tek cakes that I'd like to use as a test run while I wait for my aio jars to colonize, but tubs of that strain have been throwing green. I'd love to get a clean clone but I'm not sure if it's worth the trouble.



Avid jcm user here :heart::heart:

I change the water every grow and so basically once every 6 weeks? I keep the tubs empty and all the air stones, heaters, hoses, etc nicely cleaned, dry and wrapped in a towel placed inside of it whenever its not running. Bacteria loves standing water, particularly serratia marcescens. Having both asthma and OCD (the germophobe kind) makes a killer combo to keep everything sparkling clean and tidy :heart::oldman:


--------------------
:ancientaliens:

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Re: Official Copelandia/Panaeolus Thread [Re: astra] * 1
    #29143387 -

Glad you're feeling better sister:hug:


--------------------
And if I only could
I'd make a deal with God
And I'd get him to swap our places
I'd be running up that road
Be running up that hill
With no problems

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Re: Official Copelandia/Panaeolus Thread [Re: tree frog]
    #29143409 -

tree frog said:
Glad you're feeling better sister:hug:



Thank you, my dear sister in mycelium :hug:
Its people like you that makes life worth fighting for :heart::heart:


--------------------
:ancientaliens:

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Re: Official Copelandia/Panaeolus Thread [Re: astra] * 6
    #29143415 -

astra said:
tree frog said:
Glad you're feeling better sister:hug:



Thank you, my dear sister in mycelium :hug:
Its people like you that makes life worth fighting for :heart::heart:



Ahh :smile:  Thanks :heart:

Speaking of sisters in mycelium, fruiting some new sisters.  Pan Chlorocystis.




The white one seems to be at full maturity, so I'm not really sure when to try to print them or if they are wanting more air to open up.

At least in my tent, they have a stipe and cap shape similar to cyans.  Stipe coloration is also more similar to cyans, and cap color bisporus.


--------------------
And if I only could
I'd make a deal with God
And I'd get him to swap our places
I'd be running up that road
Be running up that hill
With no problems

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