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All-In-One Jars for Panaeolus spp. (and other exotics) * 35
    #29113303 -

Auto-Summary
The author discusses the benefits and drawbacks of using All-In-One (AIO) jars for growing oyster mushrooms, where the substrate, grains, and pasteurization are combined in a single container. AIO jars address the issues of stalled growth and contamination associated with traditional grain-based methods. However, they also present challenges, such as difficulty in spotting contamination and achieving the right substrate consistency. The author shares their preferred recipe and method for preparing AIO jars, which includes using prepped coco coir, sifted manure or compost, and grains, along with optional gypsum and calcium carbonate for pH balance. They also provide guidance on loading and pasteurizing the jars, as well as tips for monitoring and harvesting the mushrooms. With the right technique and attention to detail, AIO jars can be a reliable and efficient way to grow oyster mushrooms, as demonstrated by the author's own successful experiences and photos of their grows.

Introduction
First, I take no credit for this idea.  Folks have been running Pans in AIO bags and jars for a lot longer than I've been growing them.  And there are a couple of reasons myself and other growers prefer this method.  The first reason is that Pans have a tendency to stall on many commonly used grains.  The second reason is that manure or compost needs to be properly pasteurized or sterilized to prevent contamination.

In addition to solving both of these problems, AIO grows also change the timeline of the grow substantially.  While a half-gallon AIO jar takes a bit longer to colonize than a quart jar of grains (assuming the grains don't stall), once it's ready to spawn, it's also ready to case and go immediately into fruiting.

That said, there are a few drawbacks to AIO grows.  First, it's difficult to spot contamination.  And, if a grow is contaminated, there's no way to know without having already wasted substrate (I find a compost pile very helpful, as I can recycle contaminated subs).  Second, the consistency of the substrate takes a bit of fine-tuning, and it's easy to overload the jars.  Which makes shaking difficult down the road.  Third, AIO grows will never look as dense as a grain jar.  This can make it difficult to know when to spawn your jars.  The above drawbacks are the main reason I'm writing this tek.

Tree Frog's AIO Jars

Ingredients
2 qts of prepped coco coir
2 qts of sifted manure or compost
500ml of grains (dry volume)
Gypsum*
Calcium carbonate*
Micronutrients*

Supplies
Four half-gallon jars
Containers for measuring and mixing
A screen for the compost*

*Optional

The Grains
For this prep, I used 500ml millet, washed with a drop of dish soap.  Thoroughly rinsed and drained.  I added a bit of extra water to my substrate, to fully hudrate the millet.

But, most grain prep will work.  I used low prep here because I wanted to try it.  Currently, I'm working with mixed grains to diversify my substrate and hydrating them prior to prepping my jars (my hydration method is washed grains dumped in a bucket.  I dump boiling water on top and soak for 12-18 hours.  Learned this tek from Kirkeng and it seems to work well for most grains).



The Substrate
For Pans, I prefer black kow as my nitrogen source.  This is a well composted brand of cow manure sold all over the U.S.  I've tried garden compost (ran well but had a lot of clay) and horse manure (maybe not composted enough, the ammonia seemed to burn the fruits).  Black kow has ran the best for me, gives a good sub consistency, and is generally easy to work with.  So, that's what I prefer.

It's not super clean.  I picked this out by hand for these jars but I've since invested in some bucket screens to make things easier.



For my mix, I prefer 2 qts black kow and 2 qts hydrated coir.  Packed well during measuring.  I've used 1:1:1 black kow, hemp herd, and hydrated coir too and that also works great.  But hemp herd is expensive here and takes some extra soaking to get the moisture right (still I would recommend it if available).



Getting the prep right
Gypsum and calcium carbonate are optional, but preferable for consistency and pH balance.  One tablespoon of each sprinkled over the substrate and mixed in before adding additional water.



From here I add water, one quarter cup at a time, and continued to mix, until mix was at field capacity (took one cup of water, but this will vary depending on how wet your initial ingredients are).  Optionally, add 15ml of Jackpot micronutrients to the water prior to mixing it in.  This adds a lot of minerals without making the mix more muddy (worm castings will also add minerals but tends to get muddier than we want, but neither are necessary).

The mix should look like this.  Nice and fluffy.  Damp but not muddy.  Getting this texture right is one of the most important parts of the process.  And it's better to err on the side of a bit dry than a bit muddy.


Loading, PCing, etc.
For my jars, I prefer half-gallons.  They're easier to shake and hold enough substrate to run a shoebox of Pans.  But, I've done a lot of quart AIO jars too.  Just be mindful that you don't overfill them (600ml or less is good) and if you have extra substrate, it's better not to overfill.

Anyway, I split the mix into four jars as shown here.  The jars are filled right under the 1500ml mark (around 1400ml).  They are not compressed or tamped down.  Just split evenly and be sure not to overfill or you won't be able to shake later.



PC for 3 hours.
 

Close up of one of the cooked jars.  Millet looks nice and plump.


How I run them
After inoculation with a wedge or LI, I wait for some visible recovery, then wait one more day, then do the first shake.  Here's a couple of shots of the first recovery prior to a shake.


For my second shake I look for 30% or more growth.


Here's a jar after the second shake.  Just about finished.


And another one that's looking ready.  I like to give them another day or two past full colonization to consolidate and to be sure that everything is kosher.


Example spawning and casing
To spawn, I break and shake and dump in a tray.  This shoebox works well for my Pan Cyans.


For casing application, I ran prepped casing through a screen to get it nice and fine.


Sprinkle it on and level (but do not compress) with the back of my fingers.


And here's some shots showing casing and substrate depth.


Now I mist the casing to encourage colonization and put straight into fruiting.  Jar was put into fruiting 10 days from wedge inoculation.


Various grow pics
A jar of Ps. Ingeli prior to spawning and pinning.




Ps. Natelensis running in an AIO jar.


Some Pan. Cyans currently pinning.



And some older AIO grows done in quart jars.


Links to growlogs
Dala's ttbvi log
My original log


--------------------
And if I only could
I'd make a deal with God
And I'd get him to swap our places
I'd be running up that road
Be running up that hill
With no problems

Edited by tree frog (02/21/26 04:24 PM)

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Re: All-In-One Jars for Panaeolus Sp. (and other exotics) [Re: tree frog] * 1
    #29113316 -

:takingnotes::bump2:

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Re: All-In-One Jars for Panaeolus Sp. (and other exotics) [Re: tree frog]
    #29113318 -

Very nice! I will have to try this sometime soon. I bought a bunch of composted manure + black earth, which I hope would serve instead of the black kow. Haven't had the courage to open it up quite yet.

That being said, I did try doing a similar method using liquid nutrients in coir in PF Cakes.



BRF mixed with Hydrated Coir + Nutrients, with a casing layer of pure hydrated coir on top.

Have you done your recipe in PF cakes for skipping the bulk method?

10 Days showing Growth



I want to try a recipe like yours in the PF Cakes as well!


--------------------
I call them Huskies cause they Mush!


Huskies' Grimoire
The Official Wild Cubensis Thread
Easy Instant Pot Agar
Let's Talk Cowtek

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Re: All-In-One Jars for Panaeolus Sp. (and other exotics) [Re: tree frog] * 1
    #29113326 -

Excellent writeup TF! Thank you so much for doing this. It's super clear and easy to understand.

I can't seem to find Jackpot brand micro nutrients. It's just a 1-1-1 npk blend right? I'd like to run with additional nutes but don't want to buy the wrong stuff.


--------------------
I've got skills, they don't know where to use me
I'm like the best dressed guy at the nude beach
Nice to meet you!

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Re: All-In-One Jars for Panaeolus Sp. (and other exotics) [Re: Huskies] * 3
    #29113338 -

I ran PF cakes hydrated with compost tea and cased.



Definitely an easy way to get fruits.

And I imagine the composted manure will work well. I'm not sure what black earth is.  I ran them in horse manure as well as garden compost. My horse manure I don't think was composted enough. It seemed like the fruits got burned by the ammonia. But the consistency was good.  The garden compost ran well, but was harder to get the consistency right.  Was more muddy and had more clay.  But I suspect it also has a lot more minerals than the black kow.


--------------------
And if I only could
I'd make a deal with God
And I'd get him to swap our places
I'd be running up that road
Be running up that hill
With no problems

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Re: All-In-One Jars for Panaeolus Sp. (and other exotics) [Re: PuffM4gicDr4gon] * 1
    #29113345 -

PuffM4gicDr4gon said:
Excellent writeup TF! Thank you so much for doing this. It's super clear and easy to understand.

I can't seem to find Jackpot brand micro nutrients. It's just a 1-1-1 npk blend right? I'd like to run with additional nutes but don't want to buy the wrong stuff.



Jackpot is minerals.  If you can't find it a 1/2 cup of worm castings in the mix would also provide extra minerals.  I would probably sub out half a cup of compost for it.  That said, neither are necessary and if anyone following along wants to keep it simple, grains, coir, and compost/manure will do the trick.

Here's a picture of the jackpot though.




--------------------
And if I only could
I'd make a deal with God
And I'd get him to swap our places
I'd be running up that road
Be running up that hill
With no problems

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Re: All-In-One Jars for Panaeolus Sp. (and other exotics) [Re: tree frog] * 2
    #29113347 -

Well done, sister :heart::heart:
:raisemyglass:


--------------------
:ancientaliens:

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Re: All-In-One Jars for Panaeolus Sp. (and other exotics) [Re: tree frog] * 1
    #29113348 -

That helps with my search! Tyvm. I can also find worm casings come spring.


Edit: the picture totally put me on the right track. I found this on Amazon



--------------------
I've got skills, they don't know where to use me
I'm like the best dressed guy at the nude beach
Nice to meet you!

Edited by PuffM4gicDr4gon (02/04/25 09:51 AM)

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Re: All-In-One Jars for Panaeolus Sp. (and other exotics) [Re: PuffM4gicDr4gon] * 2
    #29113351 -

Good stuff, I'll probably try a couple of these soon. Thanks
:grampofapproval:


--------------------
This hobby has taught me many things.
Most of those things are patience.


The Hitchhiker's Guide to the Shroomery
The Official Shroomery Cultivation Museum
CakeWars
III

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Re: All-In-One Jars for Panaeolus Sp. (and other exotics) [Re: Scemilope] * 2
    #29113359 -

Very neat,this would work hypothetically for my pan cyans based on the Op too. I guess I can give it a shot, seems pretty simple to do all said and done. I'm looking for an easier path with the cyans after my first attempt at exotics with zapotecorum failed miserably.


--------------------
Endlessly endorsing empathy empirically energizing existence™

~Always practice radical kindness~
R.I.P. Betty Boop, greatest mother to ever do it.

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Re: All-In-One Jars for Panaeolus Sp. (and other exotics) [Re: QuantumZ00] * 1
    #29113445 -

Thank you for this write-up.

are your casings pasteurized or sterilized? (preferred method?)

You ever try fruiting from the jar with no casing? (then it'd truly be all in one)

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Re: All-In-One Jars for Panaeolus Sp. (and other exotics) [Re: Generic] * 1
    #29113463 -

I prefer pasteurized peat for my casing, pH adjusted.  No verm (though there's some in my pictures, it's the last batch I will make with verm in it and that's only 25%).

Fruiting from the jars with casing on top would work.  It would take a lot longer to colonize since you wouldn't want to shake it.  And you'd want to use smaller jars as Pans prefer a very thin substrate (around 1").  LC inoculation would be preferred.

Stipe was working on a tek for that, but I haven't seen anyone run it (aside from maybe that's what Huskies was mentioning up thread).  To do it well you'd want trays you can PC.  And to inoculate them well you'd want specialized lids.  But I believe it would work and that sterilized casing would run fine.


--------------------
And if I only could
I'd make a deal with God
And I'd get him to swap our places
I'd be running up that road
Be running up that hill
With no problems

Extras: Filter Print Post Top
Re: All-In-One Jars for Panaeolus Sp. (and other exotics) [Re: tree frog] * 5
    #29113522 -

Nice write up. :awedance:

You can use this for clone hunting when fruiting directly from the jar with baggy on top.

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Re: All-In-One Jars for Panaeolus Sp. (and other exotics) [Re: Baba Yaga]
    #29113679 -

Loving this! I will be trying this out soon. Thank you again, so much, for everything you do to make pans accessible.

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Re: All-In-One Jars for Panaeolus Sp. (and other exotics) [Re: Lilgrower] * 1
    #29113687 -

Is there somewhere Verm Only vs Peatmoss vs Verm/Peatmoss as a casing is discussed? I was thinking of going verm only because it doesn't need to be pasteurized and I have a lot of it at home already.

Does anyone have any thoughts on the matter?


--------------------
I've got skills, they don't know where to use me
I'm like the best dressed guy at the nude beach
Nice to meet you!

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Re: All-In-One Jars for Panaeolus Sp. (and other exotics) [Re: PuffM4gicDr4gon] * 1
    #29114096 -

Lilgrower said:
Loving this! I will be trying this out soon. Thank you again, so much, for everything you do to make pans accessible.



I hope it works well for you ❤️

PuffM4gicDr4gon said:
Is there somewhere Verm Only vs Peatmoss vs Verm/Peatmoss as a casing is discussed? I was thinking of going verm only because it doesn't need to be pasteurized and I have a lot of it at home already.

Does anyone have any thoughts on the matter?



There's a lot of folks that love verm casings.  I'm not one of them.  I'm not a fan of it being in my casings at all.

That said I have used coir and it worked for mystery sa.  And I've seen cyans top dressed with substrate do well.

Anyway if you want better advice on how to case with verm, I would suggest asking and ask quick questions get quick answers, or the miscellaneous exotics thread.  The pan thread might work too but I think Mo's of those folks are using jiffy mix or cactus soil if they're not using peat.


--------------------
And if I only could
I'd make a deal with God
And I'd get him to swap our places
I'd be running up that road
Be running up that hill
With no problems

Extras: Filter Print Post Top
Re: All-In-One Jars for Panaeolus Sp. (and other exotics) [Re: tree frog] * 1
    #29114339 -

tree frog said:
I ran PF cakes hydrated with compost tea and cased.



Definitely an easy way to get fruits.





How do you make poo tea? And do you really mean PF cakes? If I planned to go this route would I be able to go spores to field-aged hpoo tea PF cakes in an unmoded? Then open and case with pasteurized jiffy and put directly to fruiting?


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Re: All-In-One Jars for Panaeolus Sp. (and other exotics) [Re: Lilgrower] * 1
    #29114357 -

Compost tea?  It's just manure steeped in water.

And yes, that should work.  The yield isn't great though so you'd probably need to do a few to trip.

They were BRF pucks though not full PF tek.  And I don't know how well they would fruit going spores to pucks.  I know they probably will fruit but I've never done the PF tek.


--------------------
And if I only could
I'd make a deal with God
And I'd get him to swap our places
I'd be running up that road
Be running up that hill
With no problems

Extras: Filter Print Post Top
Re: All-In-One Jars for Panaeolus Sp. (and other exotics) [Re: tree frog] * 1
    #29114365 -

Awesome write-up Tree Frog! :rockon: I'll have to bookmark it for when I drum up the confidence to work with some Pans :takingnotes: Thanks for making them more accessible!


--------------------
There is no season when you are grown
You are always risen from the seeds you've sown
There is no reason to rise alone
Other stories given have sages of their own

Greene's LAGM 2025 Log!
Current Projects![/url]

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Re: All-In-One Jars for Panaeolus Sp. (and other exotics) [Re: GreeneMyco] * 1
    #29114428 -

:eek:

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