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Re: Official Copelandia/Panaeolus Thread [Re: Bra]
    #29098959 -

Bra said:
Panaeolus cyanescens from Peace River, something weird happened. It grew inside the substrate and looks like sclerotia, resembling a strange potato-like thing. It seems to be bruising. I don't know what that is! Any ideas? Has anyone experienced this before? Maybe should put this on agar later.











Never came across something like that, maybe it's caused by something else but genetics but would be cool to see how a clone does behave on agar and when grown.


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🍄PANAEOLUS IN MONOTUBS🍄
🍄OFFICIAL SEMILANCEATA THREAD🍄



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Re: Official Copelandia/Panaeolus Thread [Re: Bra] * 4
    #29098999 -

grabbed the psychedelic potatoes on accident :lol:


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Learn it, know it, live it


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Re: Official Copelandia/Panaeolus Thread [Re: mycosispeon] * 1
    #29099144 -

mycosispeon said:
Thoughts on these MiB plates? These are the T1 from BRF puck inoculated with spore. 6 days after transfer




I like the first plate.  I would probably take three transfers off of it and onto a single plate (if they're 90mm).  And then run whichever colony has the most uniform growth.

For transfer spots, I like 12 o'clock, 6 o'clock, and 9 o'clock.


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And if I only could
I'd make a deal with God
And I'd get him to swap our places
I'd be running up that road
Be running up that hill
With no problems

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Re: Official Copelandia/Panaeolus Thread [Re: Mackload] * 13
    #29099157 -

MiB got that thing thang goin on!

Using humour to cover how disturbed I am by these things, seriously, they’re frickin huge and every single one is a mutant, this tub was made with poo tea and CV



Maybe ammonia has caused this? Hpoo was pretty fresh :shrug:


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Clean MS from fresh fruits every time.
How I prep brown rice

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Re: Official Copelandia/Panaeolus Thread [Re: tree frog]
    #29099167 -

tree frog said:
mycosispeon said:
Thoughts on these MiB plates? These are the T1 from BRF puck inoculated with spore. 6 days after transfer




I like the first plate.  I would probably take three transfers off of it and onto a single plate (if they're 90mm).  And then run whichever colony has the most uniform growth.

For transfer spots, I like 12 o'clock, 6 o'clock, and 9 o'clock.


perfect. Thanks TF


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LAGM 2.024



The Hitchhiker's Guide to the Shroomery | Pastywhyte’s Ez dialed monotubs | Clean spawn checklist for the new grower | How I do Rye Berry Prep | Frank's Simple Coir/Verm Tek | Stro's Cleaning and Isolating On Agar

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Re: Official Copelandia/Panaeolus Thread [Re: 3.A.M] * 1
    #29099174 -

3.A.M said:
MiB got that thing thang goin on!

Using humour to cover how disturbed I am by these things, seriously, they’re frickin huge and every single one is a mutant, this tub was made with poo tea and CV



Maybe ammonia has caused this? Hpoo was pretty fresh :shrug:




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This hobby has taught me many things.
Most of those things are patience.

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Re: Official Copelandia/Panaeolus Thread [Re: Scemilope] * 2
    #29099245 -

3.A.M said:
MiB got that thing thang goin on!

Using humour to cover how disturbed I am by these things, seriously, they’re frickin huge and every single one is a mutant, this tub was made with poo tea and CV



Maybe ammonia has caused this? Hpoo was pretty fresh :shrug:


Yooooo these are insane looking 😭 some last of us shit lol

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Re: Official Copelandia/Panaeolus Thread [Re: CosmicCamote] * 14
    #29099870 -

These two were kind enough to stay close so I knew when they were dropping. On foil now



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Thank your postal workers when you see them. None of this would be possible without them.

Necessity is the mother of invention

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Re: Official Copelandia/Panaeolus Thread [Re: EniQma] * 2
    #29099873 -

What are these EniQma? It's a mystery to me :lol:

Nice work!

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Re: Official Copelandia/Panaeolus Thread [Re: Dkeppel]
    #29099879 -

Unlabeled bisporus! Haha
At least that’s what my labels will say


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Thank your postal workers when you see them. None of this would be possible without them.

Necessity is the mother of invention

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Re: Official Copelandia/Panaeolus Thread [Re: 3.A.M] * 2
    #29099881 -

3.A.M said:
MiB got that thing thang goin on!

Using humour to cover how disturbed I am by these things, seriously, they’re frickin huge and every single one is a mutant, this tub was made with poo tea and CV



Maybe ammonia has caused this? Hpoo was pretty fresh :shrug:


Every time I’ve had pans with stipes like this, I’ve also ended up with contamination. I believe this is an indication of some bacterial contamination competing with the mushrooms.


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Method for Small Scale Casing Pasteurization

How much really fits in a 00 capsule?

Micro and Macro Mushroom Photography

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Re: Official Copelandia/Panaeolus Thread [Re: EniQma]
    #29099896 -

Neat!

I was hoping to see some different bisporus pop up.

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Re: Official Copelandia/Panaeolus Thread [Re: RoscoeReturns] * 8
    #29099898 -

Bisporus, Washington .....a few pins from this tray, first time running these bisporus.  Though tbh, I'm not certain where the bisp Ive ran previously come from.  It could be the same source already乁⁠(⁠ ⁠•⁠_⁠•⁠ ⁠)⁠ㄏ

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Re: Official Copelandia/Panaeolus Thread [Re: stagger]
    #29099904 -

I can’t believe how translucent the cap is, never saw the gills like that through the cap


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Thank your postal workers when you see them. None of this would be possible without them.

Necessity is the mother of invention

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Re: Official Copelandia/Panaeolus Thread [Re: RoscoeReturns] * 1
    #29099906 -

RoscoeReturns said:
Every time I’ve had pans with stipes like this, I’ve also ended up with contamination. I believe this is an indication of some bacterial contamination competing with the mushrooms.


Oh, I’ve no doubt, those speckles on the stipe are meant to be a sure sign too  but I’ve had another tub from a wild cross do it also just before these guys and they’re all on different sub mixes with the only correlation being still green, stinky hpoo, I want to try and repeat it with some different cultures before crossing it off my list, or just track down some aged poo, use the same culture and find out that way.
Reckon they’re trying to pump out a flush too fast to beat contam and blowing themselves out in the process?


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Clean MS from fresh fruits every time.
How I prep brown rice

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Re: Official Copelandia/Panaeolus Thread [Re: 3.A.M] * 2
    #29100123 -

That's classic Verticillium infection. Don't see a lot of it with pans. You're probably right about the fresher poo. Likely the source. :thumbup:

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Re: Official Copelandia/Panaeolus Thread [Re: the_chosen_one]
    #29100177 -

Hello pan sages, do pans need a certain minimun temperature to colonize grain? I've had my wheat jars in a room at 64-66F and they were colonozing, but I felt it was a tad slow so just moved them to smaller room that's at 71-74F

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Re: Official Copelandia/Panaeolus Thread [Re: EniQma] * 1
    #29100297 -

Those caps are fat for Bisporus! :laugh: though Bispos do tend to have a pretty wide cap, these definitely are fatties! And major kudos for having them on foil 👏🏽 :laugh:

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Re: Official Copelandia/Panaeolus Thread [Re: the_chosen_one] * 4
    #29100303 -

the_chosen_one said:
That's classic Verticillium infection. Don't see a lot of it with pans. You're probably right about the fresher poo. Likely the source. :thumbup:


Yea you're absolutely right on that. I had revived an old syringe I had of Pan Cyan Estero and I pasteurized some sub with some horse poo that could've gone and waited a little longer, n so it was still a bit fresh. So before adding my casing to them they fruited and were shooting out for their dear life with some Verticillium on some of the stipes :/ I was able to take a few clones of the non infected ones and some scant prints though :]

So I think the overall consensus would be that too fresh of poo substrate can and does lead to bacterial infection and/or contamination


Edited by CosmicCamote (01/24/25 02:05 PM)

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Re: Official Copelandia/Panaeolus Thread [Re: CosmicCamote] * 10
    #29100406 -

23 PF Cakes of Oatmeal/BRF flour with liquid nutrient infused coir instead of vermiculite.

The top layers is pure coir to avoid contamination issues. Everything was sterilized in the PC. inoculated with LI syringe from a clean looking plate. Wish me luck!



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I call them Huskies cause they Mush!


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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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