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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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Re: Let's All Grow Mushrooms 2025 [Re: KlarnEvil9] * 6
    #29081755 -

Still no germination on my gyms and semps. ghost not even showing any signs of growth yet. they're teasing me.


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----<><><>----

⁂ tail-lights, back-pockets, rearview mirrors ⁂

⁂ guides | grows | supreme air box | sporeaddict ⁂

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Re: Let's All Grow Mushrooms 2025 [Re: fiddle_head] * 5
    #29081774 -

Azurescen from spore syringe:

1/4 germination plate -  (made t1 transfers next day)


1/8 (did more transfers)

(Uploaded the wrong pic before lol… this is the right culture)

This culture is acting nice and aggro, too :smile:

Planning on 1/10 being the day to make T2 azurescen plates and put my India Woodlover and Pan Cyan spores to agar



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When we constantly pull things apart trying to see how it works, we may end up with only an understanding of how to destroy something
- nick sand


Edited by Psicomb (01/08/25 09:45 PM)

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Re: Let's All Grow Mushrooms 2025 [Re: KlarnEvil9] * 2
    #29081775 -

KlarnEvil9 said:
Germination pics:



I was trying out some black food dye, turned out dark green/blue. So I don't really know if I'm seeing germination. Knocked on Jan 3, first signs of germ were noticed Jan 5.


They likely just blend various Red/Green/Blue food colors together instead of using actually black materials like charcoal.

That second pic looks bright pink similar to "lipstick mold"... does it look like that IRL or is it camera/food dye trickery from purple spores?

I run my plates clear and can hold up colored LEDs to backlight it if i want different contrasts...
The deep black activated charcoal plates do look really cool though i want to try those for my hopefully pretty T2-3 plates.


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:mushroom2: panta rhei :mushroom2:

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Re: Let's All Grow Mushrooms 2025 [Re: PinePalace] * 6
    #29081785 -

DV8DUG's LAGM25 Grow Log


Too late to start? I have a bunch of germ plates started 1-2-2025.

~=]o  Jack Frost x Penis Envy
~=]o  Panaeolus cyanescens ~ Weeza
~=]o  Koh Samui Super Strain
~=]o  Leucistic Koh Samui

=====================================================

Here is some packaging from two of the four types.


Here are the JFxPE with trenches that can be seen in the plates.

=====================================================
1-13-2025

Pan Cyanescens

My first time growing with anything outside of P.Cubensis. It may seem like I went a little overboard with the germ plates but I was testing to see if different nute values affected germination. As you can see plates are color coded by % value of nutes:
  • Red = 4.0%
                  10g agar powder
                  20g LME
                  500ml water
                  10 drops red food coloring
  • Blue = 1.6%
                  10g agar powder
                  8g LME
                  500ml water
                  10 drops blue food coloring
  • Black = 1.0%
                    10g agar powder
                    5g LME
                    500ml water
                    10 drops black food coloring







Here is a sample of a few plates that were looking good to me.



Now I have no experience with this mycelium type. What I have gathered from the site here is I'm looking for fast and whispy.
What does everyone think about any of these plates?

P. Cubensis ~ JFxPE

Here's a look at the trenched plates. Dirty swab. Barely making it across, I'll transfer in a day or so.



P. Cubensis ~ KSSS

Here's what I've got going to grain:


Leucistic Koh Samui

..........

2-14-2025
Oh man... I can't believe I forgot I was in this mass grow. Forgot about the thread but not about my cultures. Here's what I've got right now.

Pan Cyanescens

I have a lot of plates and have put some to poo-cakes and they are growing fast. I also have some on oats as well.


P. Cubensis ~ JFxPE

I have two shoeboxes. One created on Feb 6 and the other on Feb 8.


I have lots of grain in quarts going too...


P. Cubensis ~ KSSS

I also have 2 shoeboxes of this variety made on the 6th of Feb.


I have a lot of grain quarts for this too.


...and in single pints as well...


And I have them all tucked into a 110 qt. tub.


Leucistic Koh Samui

This is still colonizing and going well.


I also went overboard and have these cloned and/or started.
  • Ps. Cubensis var Amazon
  • Ps. Cubensis var Gandolf
  • Ps. Cubensis x7x+
  • Button mushroom from dinner just for fun


Gonna be busy...

~~~~~~~~~~~~~

2-16-2025

Spent weekend going to bulk.
  • 3 shoeboxes of Koh Samui Super Strain
  • 2 shoeboxes of Pan Cyan
  • 2 17 qt. monos of Jack Frost + Penis Envy





~~~~~~~~~~~~~~~~~~~~~~~

2-21-2025

P. Cubensis ~ KSSS

We got babies!
 


P. Cubensis ~ JFxPE

Shoeboxes doing well after casing.
 

~~~~~~~~~~~~~~~~~~~~

2-24-2025

UPDATE
P. Cubensis ~ JFxPE

First flush a little under 380 grams wet.


~~~~~~~~~~~~~~~~~~~

2-27-2025

P. Cubensis ~ KSSS

Pulled a few out because of the mess they were making but first flush!


Pan Cyanescens

Sidepinning despite liner being used...



2-27-2025 update

P. Cubensis ~ JFPE

Look at this little guy I found on a T1 plate today...



~~~~~~~~~~~~~~~~~~

3-2-2025

P. Cubensis ~ KSSS

My other 3 shoeboxes of Koh Sumui Super Strain.


~~~~~~~~~~~~~~~~~~

3-7-2025

P. Cubensis ~ JFPE

First Flush 20 qt.


~~~~~~~~~~~~~~

3-10-25

Been harvesting for a few days now. Here's some totals...

P. Cubensis var. KSSS
Tubs: 5 shoeboxes
Flush(s) to date: 1
Dry weight totals: 95 g.

P. Cubensis var. JFPE
Tubs: 4 - 20 quart and 2 shoeboxes
Flush(s) to date: 2
Dry weight totals: 233 g.

Pan Cyanescens
Tubs: 2 shoeboxes
Flush(s) to date: 1
Dry weight totals: 3 g.

P. Cubensis var. Leucistic KSSS
Tubs: 1 shoebox
Flush(s) to date: 0
Dry weight totals: 0 g.

+~+~+~+~+~+

Edited by DV8DUG (03/10/25 06:27 PM)

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Re: Let's All Grow Mushrooms 2025 [Re: DV8DUG] * 6
    #29081795 -

Never too late.


--------------------
Method for Small Scale Casing Pasteurization

How much really fits in a 00 capsule?

Micro and Macro Mushroom Photography

Can you freeze mycelium?


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Re: Let's All Grow Mushrooms 2025 [Re: RoscoeReturns] * 9
    #29081800 -

Unless it’s 2026


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Re: Let's All Grow Mushrooms 2025 [Re: Mackload] * 6
    #29081872 -

Mackload said:
already learning a really useful elementary lesson here, that spores will often be less viable after even a short time. I've only grown so far from relatively fresh prints, so took it for granted. I thought simple storage (tinfoil, baggie) and most spores would be good for years.


I believe it honestly depends on storage, but I believe ideal methods for actual long term storage of cultures is using something similar to the Cryo Vial or LI Preservation.

KlarnEvil9 said:
Germination pics:



I was trying out some black food dye, turned out dark green/blue. So I don't really know if I'm seeing germination. Knocked on Jan 3, first signs of germ were noticed Jan 5.


It looks like you have some germination near that super suspect red stuff you have going on. I'd transfer away asap.

If you want to do black agar I suggest using activated charcoal.

Non LAGM related pic for example.

-- LAGM Pic Tax: Yesterday's pictures of transfers.



--------------------
The Hitchhikers Guide to the Shroomery
:sporedrop:L.A.G.M. 2025:sporedrop:
Tico


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Re: Let's All Grow Mushrooms 2025 [Re: Axman] * 2
    #29081884 -

PinePalace said:
They likely just blend various Red/Green/Blue food colors together instead of using actually black materials like charcoal.

That second pic looks bright pink similar to "lipstick mold"... does it look like that IRL or is it camera/food dye trickery from purple spores?

I run my plates clear and can hold up colored LEDs to backlight it if i want different contrasts...
The deep black activated charcoal plates do look really cool though i want to try those for my hopefully pretty T2-3 plates.


True, I had no charcoal on hand. I'll get some more pics tomorrow of what it looks like in regular lighting.


Axman said:
It looks like you have some germination near that super suspect red stuff you have going on. I'd transfer away asap.

If you want to do black agar I suggest using activated charcoal.

Non LAGM related pic for example.

-- LAGM Pic Tax: Yesterday's pictures of transfers.




I saw that my pics kinda sucked once I got them here. I will probably redo on some different agar tomorrow.

Thanks to y'all for the eagle eyes :eyemonster:


--------------------
"For the powerful freedom-takers who rob justice from us all, that's why I keep my pistol loaded, powder, cap, and ball..."
Read more, post less!
...turns out, I'm an amateur at everything

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Re: Let's All Grow Mushrooms 2025 [Re: KlarnEvil9] * 1
    #29082037 -

Since it’s my first time using agar, I have a small question.

How much growth should I wait to see until I take my first transfer?


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Re: Let's All Grow Mushrooms 2025 [Re: Kloakk] * 6
    #29082077 -

Kloakk said:
09.01.25.

Not a lot to report, but got enough growth to do transfers, did three of both x7x+ and TWxPE6, it went pretty smooth, maybe I am getting the hang of agar :strokebeard:

Let's do x7x+ update first, clean print so far.

Before and after transfer.





The T1's




Now we move on to TWxPE6. Clean print as well.

Before and after.





The T1's



"But hey Kloakk, didn't you say three transfers of both?" Well yes, yes I did, but I lied, not about the number of transfers, but about getting the hang of my agar game(I'm sorry for lying), but you see, THIS asshole, this little shit just had to make me look like a fool to my inferiors.. Well I guess we all are rolling now, some on floor laughing, and some on the new agar plate.. :rofl:


Hope I at least did an okay job at choosing sectors.. :biggrin:



--------------------
“All Fungi are edible.

Some fungi are only edible once.”
― Terry Pratchett


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Re: Let's All Grow Mushrooms 2025 [Re: bluespirit13] * 3
    #29082191 -

bluespirit13 said:
Since it’s my first time using agar, I have a small question.

How much growth should I wait to see until I take my first transfer?



Hey, I'm on my second grow so new to agar too.

The advice I have successfully followed is:

"You would ideally want to do a transfer from a dish as soon as you see growth to avoid spreading any contaminants during the transfers. The less there is growing on your dish before the transfer the better."

Detailed here:

Stro's Cleaning and Isolating On Agar

Good luck!


--------------------
Hitchhikers Guide To The Shroomery

PrimalSoup's Acidic Tea Tek

I'm a respectful NOOB, here to learn. Please correct/critque my technique when it's questionable!



Edited by Cak Le Boof (01/10/25 05:47 AM)

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Re: Let's All Grow Mushrooms 2025 [Re: Mr Piggy] * 3
    #29082219 -

Mr Piggy said:
Just a reminder for everyone that this is the LAGM thread and is for the grow along.  If you are looking to learn about growing mushrooms or have general questions you can check in here at the quick questions quick answers thread:

Ask quick questions, get quick answers

Or click the link to the hitchiker's guide to the shroomery at the bottom of this post in my signature.

If neither of those is working for you, click the search bar in the top right corner of the screen.  I can promise your question has been asked and answered here dozens, if not hundreds of times.  Click the advanced search, set your results to five years old or newer, and get those answers.




Alright, now let's all grow mushrooms!



--------------------
🅃🄴🄰🄼 🄸🄷🄾🄿


New to cultivation or the shroomery?  Observe the hitchhiker's guide

The Hitchhiker's Guide to the Shroomery



Smellyhobbit said:
"Wiping a needle with isopropyl after flame sterilization is like shitting after you wipe your ass."

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Re: Let's All Grow Mushrooms 2025 [Re: Mr Piggy] * 6
    #29082225 -

I'm 0/6 for germing so far on my plates.  Already had one go bacterial.  I think it's time to make some BRF pucks.


--------------------
🅃🄴🄰🄼 🄸🄷🄾🄿


New to cultivation or the shroomery?  Observe the hitchhiker's guide

The Hitchhiker's Guide to the Shroomery



Smellyhobbit said:
"Wiping a needle with isopropyl after flame sterilization is like shitting after you wipe your ass."

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Re: Let's All Grow Mushrooms 2025 [Re: Mr Piggy] * 2
    #29082471 -

Haven’t checked in 2 days but I know as of then at least no germ and a few bacteria haha. Guess it’s to be expected from wild spores.
I’m contemplating brf pucks too. Just learned about them being used to fight bacteria last night.
So would you just take your ungerminated and bacterial agar and xfer like you would to a new plate?


--------------------
For it is only Darkness that crafts one to thrive in the Light. As illumination of the soul, its brilliance unmatched.
               
                    🌕🌖🌗🌘🌑🌒🌓🌔🌕
  :sporedrop:Gymnopilus subspectibilis grow log:sporedrop:             
•89g fresh subspectibilis tea•
•Rapéh Crafters•
• Gift yourself a healthy dose of Self banning•

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Re: Let's All Grow Mushrooms 2025 [Re: Soloist] * 5
    #29082559 -

three new semp plates are bacterial.



I've gone back to this one print like 6-8 times in the past few months. Quick question, how many times would you return to one print? Will next try sterile swab to puck.

Nothing else germinating yet, so might try sterile water swab for some cubes too. Hey, still only January :lol:


--------------------
Learn it, know it, live it


Edited by Mackload (01/09/25 06:18 PM)

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Re: Let's All Grow Mushrooms 2025 [Re: Mackload] * 7
    #29082871 -

I'm not making any Bacload jokes after the week I've had with agar dishes :lol:
Weird you've had so many problems with that one print.  Maybe it wasn't dry enough before it was packaged or something.  Might have a swab or two left if you need one.

You can barely make out the fine radial outgrowth from the spore clumps on my Stropharia alcis plate, but she's going finally.  Quite excited.


--------------------

THE 49TH OYSTER BUCKET TEK

FREE WILL IS A SHADOW

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Re: Let's All Grow Mushrooms 2025 [Re: alaskappalachian] * 2
    #29082873 -

cheers, thanks! nah I'm pretty sure it's my technique. cleaning some that did germinate, so fingers crossed. but that won't be in this thread.

edit: so but can you return to the same print a few times? at your own risk I guess with each revisit?


--------------------
Learn it, know it, live it


Edited by Mackload (01/09/25 08:46 PM)

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Re: Let's All Grow Mushrooms 2025 [Re: Mackload] * 8
    #29082898 -

First cube pins of 2025



--------------------
A Love Letter to New Growers
The 7 Edicts of Mushroom Cultivation as Decreed by Smellyhobbit

:kodama:


Anyone can get a canopy. But can you get what you want?

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Re: Let's All Grow Mushrooms 2025 [Re: Mackload] * 4
    #29083050 -

bluespirit13 said:
Since it’s my first time using agar, I have a small question.

How much growth should I wait to see until I take my first transfer?


I looked at your log and didn't see any pictures posted of the growth, but I personally transfer from a germination plate a few days after I see growth. Pick a few good looking sectors and put those on their own plates.

Mackload said:
I've gone back to this one print like 6-8 times in the past few months. Quick question, how many times would you return to one print? Will next try sterile swab to puck.

Nothing else germinating yet, so might try sterile water swab for some cubes too. Hey, still only January :lol:


Think about reusing prints several times like reusing a condom lol. But for real, each time you go into your print if your sterile technique isn't top notch you're adding microscopic contamination to the print, resealing it, and each time you use it the contamination compounds.

LAGM Tax:

I'm still 0/6 on my Amazonian plates, and even 0/2 on the BRF pucks. Today (amidst some other agar work) I took some swabs and wetted them with sterilized distilled water, then streaked the print, swabbed a plate and broke the tip off into the plate.



--------------------
The Hitchhikers Guide to the Shroomery
:sporedrop:L.A.G.M. 2025:sporedrop:
Tico


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Re: Let's All Grow Mushrooms 2025 [Re: Axman] * 6
    #29083164 -

I was beginning to think I'd have to go in there and do it myself.

Ancient cubensis Ecuador sending out some hyphae to check for dinosaur turds



The galindoi is still mumbling in itself in a corner.
Every few years I make like 20 prints, only to stop producing prints altogether, as there 'are plenty'. Bad habit.


--------------------
No nation, no border
Fight law and order


Species grown so far

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