AspectOfTheCreator said:

80% failure rate.








I dont have pics of the agar but it was just generic, clean round symetrical LMEA plates that dont contam even when saved for months after use. As a test, Ive kept blank agar plates open while doing a2g and contam never shows up even months later. It will show up if i cough on it. All my jars colonize the same, look the same. They always get trich right when fruiting should start. Ive used sfd stickers, sfd discs with atv glue. My success rate with agar is 100%. My jars always colonize within 2-3 weeks. Always look clean. No grains against glass. Before the shake prior to spawning I check the headspace above the grains for any stray uncolonized grains against the glass or inner lid. I also remove the agar wedge with a spoon. I spawn to coco bliss coir prepped via bucket tek with 4.6x its mass in water. 1:2.5:0.5 (1 qt spawn mixed with 2.5 qt coir pseudo cased with 0.5 qt coir).

I delt with this issue when first coming back to cult except had 100% failure. Went to about 100% success (all spawn yielding 1 oz dry or more) despite not really changing much. Now, inexplicably back to huge failure rate. I use pennington wbs prepped fooman style PCed at 18 psi for 2 hours. One piece metal lids with sfd sticker or atv glued sfd. Spawn to just plain coir. Everything always seems perfect. Everything colonizes well, surface conditions are good. Then bam, right around the time I expect fruits I get trich.

People are going to tell me my spawn is off but it looks no different from when I had high success rates spawning PE to a 1:6 ratio.

On the bright side, over the past 2 years I have grown more mush then I know what to do with in 10 life times. I could get out the game for awhile but I genuinely enjoy the hobby and would like to figure out why I cant grow consistently even at this point. After my past success throwing up canopies of harder to cult mush like pans, nats, enigma, and penis envy.

If anyone has any out the box ideas im open to listen. Someone recommended I spawn infront of a flow hood. I like the idea but am not open to purchasing one.

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malvaneglecta said:

Some yellowing at the bottom of my jar where there are burst grains. Is it probably contaminated and should I toss it?

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Kloakk said:

I've read about some people being concerned that after PC'ing, during cool down, there is air finding it's way inside the jar, is that a thing to worry about? I also read that leaving the jars in the PC until they are room temperature would be enough to avoid contamination's, would it help?

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GenesisCorrupted said:

It’s not gonna help prevent contamination to let them cool in your pressure cooker. The pressure cooking is what’s going to take care of the contamination. You leave them in there to prevent some condensation and let them cool.

You’re supposed to leave them unscrewed enough that air transfers inside of them. But if a vacuum does form. Just be sure to open them inside of the SAB or in front of the FFU.

If you are prepared for it, this also does not matter.

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Yeah, I was talking about after PC'ing with unmodded lids. The lids would be unscrewed before and after PC'ing. Hot air and liquid will expand while heating up and shrink during cool down. What I read was that during cool down it's inevitable that air will be sucked in the jar(s) because air and liquid is shrinking.

The argument for lids with SFD's was that one could close the lid while the jar was still hot and that during cool down the air sucked in would be filtered. Those who went with the unmodded lids, answered that if you leave the jars to cool inside the PC it was not a problem because the air in the PC is pretty sterile and contams finding they're way under the rocker, get inside the PC, under the foil and in to the jars was not likely.

I was just wondering if any of this is true and if it is, what all of you do to deal with it, and why you like the modded or unmodded lid. I am John Snow, I know nothing

ThurmanMurman said:

Dont tighten lids when the jars are hot. When you go to unscrew it, it will pull air into the jar, and possibly/probably contamination with it.

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Noted, but the same amount of air will fill the jar(s) during cool down either way. It makes sense that one "big" intake of air(if I where closing the lids while hot) would increase the chance off contamination rather then just let them cool with the lids unscrewed and let air and liquid shrink over time..

I would like to know if this is something to be thinking of and what measures and methods people are doing to minimize the chance of contamination at that stage

DERRAYLD said:

If you have modified lids you should tighten them before pc.

I wait for my gauge to drop to zero,  remove the hot jars with a silicone glove and give them a good shake.

That way I can run multiple cycles faster.

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GenesisCorrupted said:

The only things that would be losing their structure, shouldn’t be in a pressure cooker to begin with.
Pp5 and autoclavable only

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DERRAYLD said:

If you have modified lids you should tighten them before pc.

I wait for my gauge to drop to zero,  remove the hot jars with a silicone glove and give them a good shake.

That way I can run multiple cycles faster.

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GenesisCorrupted said:

If there’s a hole in the bag covered by a filter, then the bag is not gonna inflate like a balloon.

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I've read several threads about bags popping, both pre sealed and not. I'm not going to argue, but I read several people talking about how pressure might be compromising the filter patch and was wondering if this is a concern with SFD modded lids, but with the answers I'm getting I guess it's not a issue

DERRAYLD said:

I've never had an issue with sfd, micropore, etc.

I use the same stickers and mp tape for multiple runs, I think my current jars have the same filters and mp tape for the last year.

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Sounds good, thanks

FluffyShrooms said:

Hello, I've been feeling pretty confident about my process so far but would love to hear any input. I got 18 jars going right now and these 6 are the first batch that I'm hoping to s2b as soon as the last jar finish colonizing (I shook it at 70% yesterday)

My only concern is that one of the jar has a tiny cluster of uncolonized grain right on top and the top of one of the jars looks really fuzzy (making me think it might be contaminated?). This is my time growing and I've looked at tons of pictures/research on reddit/shroomery but for some reason I still feel nervous.

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KlarnEvil9 said:

ThurmanMurman said:

lifescientist said:

Haven't read it, just following Frank's guide but fruiting and stuff has me confused. Where can I find it?

Edit: https://www.shroomery.org/forums/showflat.php/Number/22337800

This one?

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No. I really need to figure out how to link shit in a post.

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Easy peasy. Copy the link, then highlight the text you want the link to be, click the link icon - has a globe on it, just left of the anchor. Paste your copied URL in the box and click ok. The text you selected will now be highlighted and clickable.

Example - HHG link

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