TheQueenOfDreams said:

I’m a newer hobbyist who jumped in with a lot of enthusiasm, but not a lot of education. I’ve been trying to figure things out on my own, but without a trained eye, it’s hard to know what I’m seeing. I recently started asking some questions and was led here.

I’m hoping someone will look at this plate and tell me if it’s clean, and okay to spawn. There is a yellow brown dot that looks like contam but may be myco metabolites, as I forgot about this plate for several weeks and it’s sat out at room temp most of the summer.

MSS > agar

si=51IIRcOXoc_7KnIl

---

TheQueenOfDreams said:

I’m a newer hobbyist who jumped in with a lot of enthusiasm, but not a lot of education. I’ve been trying to figure things out on my own, but without a trained eye, it’s hard to know what I’m seeing. I recently started asking some questions and was led here.

I’m hoping someone will look at this plate and tell me if it’s clean, and okay to spawn. There is a yellow brown dot that looks like contam but may be myco metabolites, as I forgot about this plate for several weeks and it’s sat out at room temp most of the summer.

MSS > agar

si=51IIRcOXoc_7KnIl

---

TheQueenOfDreams said:

I had to watch a few videos to understand what was meant by leading edge and how to decide where to section from, and now I understand why it’s fucked. I decided to start over this morning from the MSS and keep a better eye on it this time. When should I look at it or expect it to be ready to take a sample from?

Regarding my crazy agar plate, just so I can learn from it, is that amber droplet likely contam or just metabolites?

Thank you for the feedback!!
Sue

---