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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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Re: Ask Quick Questions, Get Quick Answers [Re: Blueberry Muffin]
    #28891804 -

Blueberry Muffin said:
How did you make your agar? Looks very strange. First plate has bacterial contam at 9 and the bottom two seems like mold to me


Yeah I spotted the bacteria. The agar was made by someone else using:

Sterilised water
Medical grade agar-agar
Food grade light malt extract (LME)
Activated charcoal


--------------------


Here to unlearn everything I thought I knew.

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Re: Ask Quick Questions, Get Quick Answers [Re: Polkahontass]
    #28891850 -

Ah ok. That would make more sense. My fault.


--------------------


Hitchhikers Guide to The Shroomery

Shine on forever, shine on benevolent son
Shine down upon the severed
Shine until the two become one

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Re: Ask Quick Questions, Get Quick Answers [Re: ThurmanMurman] * 1
    #28891860 -

Earshot, you bought those prepoured?

I'd ask for a refund, those are terribly poured plates and they're not even mixed properly.

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Re: Ask Quick Questions, Get Quick Answers [Re: EarShot] * 1
    #28891884 -

If you can, make your own agar and skip the fancy stuff. Tap water, 2% agar, 1-2% lme. Medical grade not needed.


--------------------
The Hitchhiker's Guide to the Shroomery

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Re: Ask Quick Questions, Get Quick Answers [Re: Blueberry Muffin] * 1
    #28891898 -

Blueberry Muffin said:
If you can, make your own agar and skip the fancy stuff. Tap water, 2% agar, 1-2% lme. Medical grade not needed.



I find that with agar, distilled or R/O water works better. I often get the LME coagulating using tap. I also like a softer agar and use 1.5% agar 1% LME

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Re: Ask Quick Questions, Get Quick Answers [Re: AzureCultivator]
    #28891932 -

AzureCultivator said:
RockinRobot said:
ThurmanMurman said:
Whats the best way to top fruit a single bacterial quart of spawn? I rubberbanded a gal ziplock over the top and punched a few holes in it, but there was no condensation build up after about 12 hours, so I abandoned that idea and put the lid back on until I came up with a better plan. Cased with about an inch of coir as well. Ive seen the teks, but putting it in a tub is going to make it more difficult, without multiple jars helping to create a climate, right?


A Shoebox is the perfect tub for fruiting questionable spawn. 1qt spawn to 2 qts coir with maybe another 1/2 qt coir as a pseudo case to cover any exposed grains.



My favorite Shoebox TEK


Yeah! Thanks for dropping a link with the detailed answer, RR. A bow to the skilled response



RockinRobot is now the new Designated RR! Congratulations :congrats:

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Re: Ask Quick Questions, Get Quick Answers [Re: LewDoja] * 4
    #28891943 -

LewDoja said:
AzureCultivator said:
RockinRobot said:
ThurmanMurman said:
Whats the best way to top fruit a single bacterial quart of spawn? I rubberbanded a gal ziplock over the top and punched a few holes in it, but there was no condensation build up after about 12 hours, so I abandoned that idea and put the lid back on until I came up with a better plan. Cased with about an inch of coir as well. Ive seen the teks, but putting it in a tub is going to make it more difficult, without multiple jars helping to create a climate, right?


A Shoebox is the perfect tub for fruiting questionable spawn. 1qt spawn to 2 qts coir with maybe another 1/2 qt coir as a pseudo case to cover any exposed grains.



My favorite Shoebox TEK


Yeah! Thanks for dropping a link with the detailed answer, RR. A bow to the skilled response



RockinRobot is now the new Designated RR! Congratulations :congrats:



DO NOT equate me to RR EVER.....

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Re: Ask Quick Questions, Get Quick Answers [Re: RockinRobot] * 1
    #28891956 -

Too late. No worries, though. I don’t know the old RR — rip, old brother


--------------------
Hitchhiker’s Guide to the Shroomery “Clean spawn is easily the most critical component of the entire cultivation process.”
Drink the Water of Life and become Paul Muad’Dib.
Remember to surrender.

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Re: Ask Quick Questions, Get Quick Answers [Re: RockinRobot] * 5
    #28891957 -

Why, is it because you have up to date info?


--------------------
🅃🄴🄰🄼 🄸🄷🄾🄿


New to cultivation or the shroomery?  Observe the hitchhiker's guide

The Hitchhiker's Guide to the Shroomery



Smellyhobbit said:
"Wiping a needle with isopropyl after flame sterilization is like shitting after you wipe your ass."

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Re: Ask Quick Questions, Get Quick Answers [Re: Mr Piggy]
    #28892027 -



Made some grain jars over the weekend and I think they’re all looking kinda sketchy on the bottom… could it be burst grains or should I just toss them. I’m leaning towards the WBS I used being way too old and just buying some other grain at the feed store

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Re: Ask Quick Questions, Get Quick Answers [Re: Mr Piggy] * 2
    #28892044 -

Mr Piggy said:
Why, is it because you have up to date info?


That and the fact that just like Stamet's he went commercial. Plus many of his methods have been debunked.

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Re: Ask Quick Questions, Get Quick Answers [Re: NotWimbo]
    #28892064 -

NotWimbo said:


Made some grain jars over the weekend and I think they’re all looking kinda sketchy on the bottom… could it be burst grains or should I just toss them. I’m leaning towards the WBS I used being way too old and just buying some other grain at the feed store


Looks like a lot of burst grain to me


--------------------
Where to start?

Are your plates clean?


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Re: Ask Quick Questions, Get Quick Answers [Re: NotWimbo]
    #28892123 -

NotWimbo said:


Made some grain jars over the weekend and I think they’re all looking kinda sketchy on the bottom… could it be burst grains or should I just toss them. I’m leaning towards the WBS I used being way too old and just buying some other grain at the feed store


They were overhydrated or left in the boil pot too long.  Age has nothing to do with it, it was your process.  I just made perfect spawn with five year old WBS.

Walk us through your process start to finish.


--------------------
🅃🄴🄰🄼 🄸🄷🄾🄿


New to cultivation or the shroomery?  Observe the hitchhiker's guide

The Hitchhiker's Guide to the Shroomery



Smellyhobbit said:
"Wiping a needle with isopropyl after flame sterilization is like shitting after you wipe your ass."

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Re: Ask Quick Questions, Get Quick Answers [Re: Mycodemus]
    #28892134 -

Bump :smile:
Mycodemus said:
I’ve got 7 grain jars and some quite old plins (plates are about a year old). Please help me decide what to send (if anything)— or failing that, what I should transfer to a new plate. Thanks!

Semperviva:
Three plates here, one has just little knots, one has a tiny plin, and one has a nice cluster which I’m not sure is bruised or contammed. The cluster looked fine a week or two ago and I don’t remember that big bit of condensation.



Natalensis:
The uncut plate seems to have contam on one side. The cut plate looks very dry.



P. Mexicana:
I transferred part of a plin or maybe it was a plone (plate stone lol). However as you see, it never took off one way or another for some reason. The transfer was taken from the large plin which is now overgrown by myc, at about 7:00.





Ecuador:

Very old and dried out, but seems clean to me I think.




--------------------


An hour and a half later, I was beginning to feel definite effects
Subjective Effect Index: comprehensive map of all potential psychoactive experiences

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Re: Ask Quick Questions, Get Quick Answers [Re: AzureCultivator]
    #28892154 -

Wait, RR died? Ive been away over a decade, until recently...


--------------------


Hitchhikers Guide to The Shroomery

Shine on forever, shine on benevolent son
Shine down upon the severed
Shine until the two become one

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Re: Ask Quick Questions, Get Quick Answers [Re: ThurmanMurman] * 1
    #28892201 -

I dont think so? Last I heard he was in a court battle trying to save his land from being stolen. Im out of the loop though so take that with a grain of salt.


--------------------



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Re: Ask Quick Questions, Get Quick Answers [Re: Mr Piggy]
    #28892212 -

Mr Piggy said:
NotWimbo said:


Made some grain jars over the weekend and I think they’re all looking kinda sketchy on the bottom… could it be burst grains or should I just toss them. I’m leaning towards the WBS I used being way too old and just buying some other grain at the feed store


They were overhydrated or left in the boil pot too long.  Age has nothing to do with it, it was your process.  I just made perfect spawn with five year old WBS.

Walk us through your process start to finish.


I did the fooman prep I was linked here. Boiled some water to a rolling boil, took it off heat, poured in my WBS and covered, let it sit for a little more than an hour (I’d say 70 or so minutes), pour it into a strainer and shook it, let it sit there for 30-ish minutes shaking off excess water at the beginning and end, then spooned into jars and pc in the instantpot (I know it’s not optimal but it’s what I got) for 3 hours with aluminum foil over the lid (I used the easy-af polyfil lids)

Edited by NotWimbo (07/29/24 03:20 PM)

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Re: Ask Quick Questions, Get Quick Answers [Re: NotWimbo]
    #28892215 -

Shit also can I knock these up like this or burst seeds will fuck up my chances?

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Re: Ask Quick Questions, Get Quick Answers [Re: ThurmanMurman]
    #28892233 -

ThurmanMurman said:
Wait, RR died? Ive been away over a decade, until recently...


I just meant he was gone from the Shroomery not the Earth. rip old RR


--------------------
Hitchhiker’s Guide to the Shroomery “Clean spawn is easily the most critical component of the entire cultivation process.”
Drink the Water of Life and become Paul Muad’Dib.
Remember to surrender.

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Re: Ask Quick Questions, Get Quick Answers [Re: AzureCultivator] * 3
    #28892237 -

As long as you're using clean innoculant you'll be fine.  Just could use more drying before loading because wbs isn't nearly as forgiving as oats, rye, etc when running the grains before all surface moisture is evaporated/drained.  Just don't use spores if that's what you're thinking or you're likely to have some heartache.


--------------------

THE 49TH OYSTER BUCKET TEK

FREE WILL IS A SHADOW

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Shop: Myyco.com Isolated Cubensis Liquid Culture For Sale   Original Sensible Seeds Autoflowering Cannabis Seeds   Sporeworks.EU Spores for European Microscopy   Mushroom-Hut Substrate Bags   North Spore Injection Grain Bag

Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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