ArkaNaut said:

Soloist said:

AzureCultivator said:

tHeMaGe said:

Keep your finger on the off button toward the end if you don't want a mess in your microwave. I also put the jar in a mic safe bowl. Once you clean up an agar explosion once, you'll never want to do it again

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Holy shit

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Avoided a major mishap not that long ago -had a media bottle full of bubbling hot agar crack in my hand mid-shake


Still poured those fucking plates though.
(Thankfully I was wearing thick ass neoprene coated gloves and got away scott free)

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Damn!  It’s a testament to how tough that glass is.  Mine had cracked almost completely around.  You’re damn right I poured the plates.  Toasted my fingertips through the heat resistant gloves, but that agar got on those plates!

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AzureCultivator said:

Holy shit

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Quote:

PF Pucks and Cakes
Cakes are all B+
From 1 day after inoculation to 4 days after inoculation:


Albino MVP                      Azurescens                        Tampanensis                    Avery's Albino                    B+

Tub Pins Coming along nicely

B+                                      APE

Might have some pins forming in the bags as well

B+                                      APE

Finally, I don't have cellophane to use at the moment, so I micropored my inoculated plates together.  Nothing to see on these, probably leave them alone a couple of days before checking them.  Will likely cellophane them closed then.

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Goatrider said:

You´re absolutely on track mate.
Damn good work

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monkey_accident said:

nice work

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Thanks!

Yesterday I did a ton of agar transfers, advanced all the projects.  Didn't take pics of everything, it was a lot, but once there's some growth worth showing I'll do a little roundup. 

I've harvested the first flushes of B+ and APE.  Both subs have been abused Beef style  soaked overnight and are set up for another flush.  If I get nothing that's fine, I wasn't even expecting a first flush.  Edit 6/11/24 - already getting pins on the B+

Flushwatch 2: The Flushening

                                            06/10/24                          06/11/2024



Tried to get spore prints from some APE caps, but as is their wont, they dropped nothing.  I cut em and smashed em and put a drop of water on each one, but I'll be suprised if I get spores from them.  Doesn't really matter, I have other genetics and these were all mutated AF, but worth a try.  I'll toss em in the dehydrator whether they drop any spores or not after the next 24 hrs. Edit 6/11/24 - still no spores, probably not getting any.



Been posting a lot in the Q&A forum.  Really helping me tune my focus.  For some reason I had become enamored with the idea of using popcorn as my first grain spawn, but I've been set straight and will be prepping rye instead.

I have a bunch of 4oz jars set aside, though I'm not entirely sure what I'm going to do with them.  My PFCakes with B+ were inoculated on the surface of the verm, so I just had some sad grey mycelium on top.  I put them under the SAB and shoved all the sad myc down to the BRF layer, will see if it catches.  So I could make a bunch more PFCakes, but I don't really know what I'd do with them afterwards.  Don't really feel like making a shotgun fruiting chamber, I'm pretty happy with spawning to bulk.  I suppose I could spawn them to bulk, or use them to inoculate grain spawn as an alternative to Agar, but I just don't know.  Now that I'm becoming fluent with Agar I just don't know what to do with PF substrate.  Kind of skipped it.

Speaking of, I have three nearly complete PFPucks, trying to decide what to do.  I did the Josex Poke just for practice onto some agar, and also to get some of this B+ strain onto Agar, but now I just have these pucks.  Probably put them into grain spawn once I have it.  Will ask the Q&A if it's worth my time to top them and fruit them.  I'd expect small yields, but since it'll be awhile until I have grain spawn ready and enough colonized agar plates to inoculate them, it might be nice to have a small crop of fruits within the next week or so.

Maybe that's the thing to do?  Have pucks going since they're so low maintenance and just keep top fruiting them to have a low background yield all the time until I'm caught up with grain spawn and monotubs?

Edit 6/11/24 - after talking to Q&A folks, and reviewing the Josex Poke and Pucks post, I can expect these pucks to pin without casing, so I'll just watch em.  The PFCakes still have no sign of recovery.  I'll give em a little while longer, but if there's nothing, I'll combine two doomed projects and dump all this PFSubstrate onto the two doomed sad bags I have and see if that does anything.

Quote:

How long would I have to PC a dead squirrel?

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wbs smells bad enough i cant imagine

tyga772 said:

Quote:

How long would I have to PC a dead squirrel?

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wbs smells bad enough i cant imagine

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I would endure it to bring forth the sentient, ravenous fruits that will destroy us all. 

cl_thirtyfour said:

excellent work, love to see the progress, keep it up!

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BeefSupremeJr said:

man! budy busy busy!  you have so much energy its incredible!  keep going man.  this is great shit

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Thanks!  I really do appreciate the encouragement.

First a gross picture - I plopped a shroom end onto agar to see what would happen.  This happened.  Haven't researched to see exactly what the fuzzy white mold with black specks is, but methinks it ain't a cube.


I made a streak plate from wiping the mycelium with my inoc loop, and appear to have grown a whole bunch of bacteria.


I had spores on my gloved fingers, so I rubbed them on a plate and got this:


Thinking ahead, I had also used the inoc loop to transfer a clump of spores onto another plate and streaked it, and seem to have grown a nice selection of trich and bacteria.


In other relatively bad news, my second flush of Vogon APE grew a spot of trich, which I tore out.  The blob of bluegreen at the bottom I had thought was blue bruising from when I abused it under the faucet, but now it's pretty clearly trich as well. There are a few hopeful pins in the center there, so I'll allow them to race against time, but won't be lifting the lid indoors.


The Vogon B+ is attempting to squeeze out a couple more shrooms.  I fed it some water in hopes that it would help.  No apparent contam right now, but I'm leaving the lid on until it either contams or produces something harvestable.


My vogon bags continue to consolidate, and the myc is matted on top, so I started spritzing to perhaps invoke some proper surface conditions for fruiting.  Clearly the one on the right is overwatered.  I'll let it stew for the evening and maybe soak up some of that water, then tomorrow I'll drain off what's left.


The first Avery Albino plate is curling up.  The transfers I had taken from it are plenty happy though, and will perhaps be ready for grain when that's available.


The Dirty Teacher continues to look perfectly normal.  Not sure why the bag looked like such utter garbage, but the transfers are doing well, too, so I may be able to spawn and harvest some classic GTs in the near future.


I realized that I had done my josex poke incorrectly - without a syringe of sterile water to spit out the punch biopsy, so two of the poked plates are still blank looking, but at least one looks OK.


Rescued samples of Vogon APE and their transfers are growing slowly but surely.



The FP+ from the lovely Beef is chuggin along just fine, and the baggie has no sign of contam within.  Anyone have a guess for what the little dark circle blob on "B" is?  I may send C to grain after transferring from each for a nice monocolony, only because I'm really excited after finding out what FP+ actually is:


And in "back from the dead" news, now three of my seven PFCakes have come back from the dead:


My cubensis PFPucks are continuing to consolidate, still no sign of pins.


Nothing continues to visibly grow on the "Control" puck.  Azurescens seems reasonably happy on the PFSubstrate:


Tampanensis might be making a sclerotia! Sclerotium?  Scrotum?


I've been hesitant to start making grain spawn for no particular reason.  I can fit seven quart jars in my 23Qt Presto, so I filled up three quarts with triple washed fancypants rye grains and put them in the bathtub for a nice soak. 


Goodnight, Rye, I will see you in the morning and almost but not quite boil you for several hours per Beef's Wheat tek

Goodnight to you too, Shroomerinos.

BeefSupremeJr said:

wtf why does your fp+
culture look better than mine?!

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It was sent through the post office, which means it was squashed flat, shipped in a dreadfully unsterile container, and probably irradiated, and the postal workers opened it up and looked at it under a microsope, called the secret service who taste tested it and decided it was ok to go, then sent it to me.  It was so improbable that it would even arrive that it can't help but thrive! 

BeefSupremeJr said:

wtf why does your fp+
culture look better than mine?!

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Herme5 said:

H

BeefSupremeJr said:

wtf why does your fp+
culture look better than mine?!

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He made it with love

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