Home | Community | Message Board

PlanetSpores.ca
This site includes paid links. Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: Mushroom-Hut Grow Bags   North Spore Injection Grain Bag   Unfolding Nature Unfolding Nature: Being in the Implicate Order   Original Sensible Seeds Autoflowering Cannabis Seeds   Myyco.com APE Liquid Culture For Sale   MagicBag.co All-In-One Bags That Don't Suck   Sporeworks.EU Spores for European Microscopy

Jump to first unread post Pages: | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 |  [ show all ]
Re: New Hitchhiker Learns Not to Panic [Re: ArkaNaut]
    #28795216 -

I haven’t gone down this path but I’ve seen the warnings about PCing agar too long. It turns into what you have

No worries. Pitch it and start fresh. You’ll get the feel real quick


--------------------
Hitchhiker’s Guide to the Shroomery “Clean spawn is easily the most critical component of the entire cultivation process.”
Drink the Water of Life and become Paul Muad’Dib.
Remember to surrender.

Extras: Filter Print Post Top
Re: New Hitchhiker Learns Not to Panic [Re: AzureCultivator]
    #28795220 -

Awesome, thank you.  It's been at 190 for awhile now, and although it's kind of translucent, this seems harder than it should be based on what I've read.  Now I just have to dig it out of these bottles :laugh:

Extras: Filter Print Post Top
Re: New Hitchhiker Learns Not to Panic [Re: ArkaNaut]
    #28795329 -

A water bath alone is not enough to get chilled agar back to liquid ime. When I'm ready to pour I microwave again a few minutes at a time with cap loose. Mic, shake, mic, shake until liquid and then put into water bath at 120 to cool. Drink some beers and pour some plates...

Keep your finger on the off button toward the end if you don't want a mess in your microwave. I also put the jar in a mic safe bowl. Once you clean up an agar explosion once, you'll never want to do it again :smile:

Extras: Filter Print Post Top
Re: New Hitchhiker Learns Not to Panic [Re: tHeMaGe]
    #28795336 -

tHeMaGe said:
Keep your finger on the off button toward the end if you don't want a mess in your microwave. I also put the jar in a mic safe bowl. Once you clean up an agar explosion once, you'll never want to do it again :smile:



Holy shit


--------------------
Hitchhiker’s Guide to the Shroomery “Clean spawn is easily the most critical component of the entire cultivation process.”
Drink the Water of Life and become Paul Muad’Dib.
Remember to surrender.

Extras: Filter Print Post Top
Re: New Hitchhiker Learns Not to Panic [Re: tHeMaGe]
    #28795365 -

tHeMaGe said:
A water bath alone is not enough to get chilled agar back to liquid ime. When I'm ready to pour I microwave again a few minutes at a time with cap loose. Mic, shake, mic, shake until liquid and then put into water bath at 120 to cool. Drink some beers and pour some plates...

Keep your finger on the off button toward the end if you don't want a mess in your microwave. I also put the jar in a mic safe bowl. Once you clean up an agar explosion once, you'll never want to do it again :smile:



Cool, I’ll give it a shot before I dump it all and restart. Thank you!

Extras: Filter Print Post Top
Re: New Hitchhiker Learns Not to Panic [Re: AzureCultivator]
    #28795369 -

AzureCultivator said:
I haven’t gone down this path but I’ve seen the warnings about PCing agar too long. It turns into what you have

No worries. Pitch it and start fresh. You’ll get the feel real quick



Based on your username…do you have any recommendations specific to azure cultivation? 

I’ve noticed some folks incorporate wood, others seem to be fine fruiting from grains just like a cube.  Haven’t done a deep dive into the forums on this yet, but I did put some lc on a pfpuck to see what will happen…

Extras: Filter Print Post Top
Re: New Hitchhiker Learns Not to Panic [Re: ArkaNaut] * 1
    #28795374 -

bud just so you know, you were nominated as noob of the week by nichrome.  i was gonna do it too but ive been busy this week.  either way.  thanks for everything bud.

congrats bud.  take a bow

https://www.shroomery.org/forums/showflat.php/Number/28711645/fpart/3/vc/1

Extras: Filter Print Post Top
Re: New Hitchhiker Learns Not to Panic [Re: BeefExtreme]
    #28795417 -

Thank you!  One of the best parts of my day is checking in on the forum to see if anyone has commented.  This is such a helpful, welcoming community.  There’s a great atmosphere and culture, like we’re all on some wild journey together across the cosmos.  Now I’ve got my towel, I’ve learned not to panic, and I stopped listening to Vogon poetry.  I got kicked out the airlock, but the Heart of Gold plucked me out of the vacuum one second before my lungs exploded.

Extras: Filter Print Post Top
Re: New Hitchhiker Learns Not to Panic [Re: ArkaNaut]
    #28795426 -



--------------------
Question everything, it's very responsive.


Culture Storage

Extras: Filter Print Post Top
Re: New Hitchhiker Learns Not to Panic [Re: Nichrome] * 1
    #28795441 -

fuck ya! Youre killin it bud!  Thanks for buying a supporter acct  too man. 

nichrome lol nice new avarar.

Extras: Filter Print Post Top
Re: New Hitchhiker Learns Not to Panic [Re: BeefExtreme]
    #28795453 -

Congrats man! Keep it up, you'll have fruits before you know it.


--------------------
🅃 🄴 🄰 🄼    🄲 🄻 🄸 🄽 🄶 🅆 🅁 🄰 🄿

"The lips of wisdom are closed, except to the ears of understanding." - The Kyballion, Three Initiates

Advanced Research of the Chronological History of Artifical Intelligence X

Extras: Filter Print Post Top
Re: New Hitchhiker Learns Not to Panic [Re: ArkaNaut]
    #28795643 -

Auto-Summary
The post discusses cultivating Psilocybe azurescens outdoors, referencing various sources and personal experiences. Key points include:

1. Azurescens can be cultivated without lab work, using a method described by Jochen Gartz.
2. European growers often start with spawn production in spring, resulting in a first harvest in the fall.
3. Clean, dry wood chips are recommended for feeding and covering the outdoor bed.
4. High humidity is essential for spore germination, and temperature affects speed but not overall success.
5. Azurescens requires several weeks of cold weather (0-10°C) for fruiting.
6. Watering should be automatic, as the mushroom likes very wet conditions.
7. Psilocybe cyanescens is considered the best variety for home cultivation, growing on Alder, Maple, and Fir, and taking two years to produce a harvest.
8. Azurescens and other wood-loving psilocybes, like Stropharia rugosoannulata, can be grown using similar techniques.
9. Azurescens is difficult to grow indoors, with the most successful indoor grow reported in about 3 months using a specific tek by user abert.

Azurescens Notes

Dreaming of doing an outdoor grow, with mushrooms poking up amidst the garden veggies.  Here for my own records and anyone curious are excerpts and thoughts from several sources on shroomery about growing azurescens.

Anno already did a better job of this than I am about to do below, so if you're really curious go check out their thread here: Psilocybe azurescens outdoor cultivation
Sadly, the links in the article to more info are dead, a mere 20 years after the post was created.  The MushMush link isn't dead, but it's a barebones website for a netherlands group that produces sclerotia on an industrial scale.  Neat but not helpful for me.

Anno shows what is possible with outdoor patches in this thread from 2020: Psilocybe azurescens 2020

Quote:
Psilocybe azurescens cultivation in the Mushroom Info section of the Shroomery says:

Jochen Gartz recently described a "new method of mushroom cultivation in N.
America", which can indeed do without any labwork (6).

Some european growers prefer to start with a (onetime) run of spawn-
production (agar-dishes, glasses of oats/rye/barley, then glasses/bags of
wood-chips
) in spring, which usually results in a first harvest in fall, and
a mushroom bed that can be enlarged both in area and yield in the next years
and very probably be maintained far beyond human lifetime. Clean wood debris
(like from a planing machine, sawdust does not work) seem to work even better
than freshly chopped wood. Although Psilocybe cyanescens seems to be able to
fruit on plain wood without any casing, it is advisable to supply the bed
with enough soil (and seeds), and leaves and larger branches, as the
mushrooms really love to grow in between and under grass or other plants
.

The mushroom-beds obtained can be left on their own most of the year and (in
a good location) need attention only at harvesting time. Outdoors un-
favorable weather may of course diminish or prevent a year's harvest, but as
this cannot hurt the mass of underground mycelium, the shrooms will just make
up for it next season.
...
There seems to be hardly any difference between different MEA and PD(Y)A-
formulas or between cheapest agar-agar or a highly purified quality, or
whether pH is adjusted precisely, phosphate or trace elements are added or
not. High humidity is absolutely essential for spore germination; within a
reasonable range (maybe (5)10-30(40) degrees Celsius for PCY-AO) temperature
has effect on the speed but hardly on overall success.
...
PCY-AO seems to like barley, oats, rye, wheat or rice alike.
...
My personal favourite mixture is
half oats (least "sticky"), a quarter millet (often used for commercial spawn
production. Advantage: many small granules), rest: mixture of others as
available. I cut fully grown agar cultures (and only perfect ones) with a new
blade (PCY-AO mycelium is pretty thoughl; shows strong blueing here) into as
many pieces as possible (hundreds at least) and work these thoroughly using a
whole agar plate per jar. PCY-AO also seems to like nearly any kind of wood:
fir, larch, beech, birch, willow and others, did not show any difference in
growth. (At least) for spawn-making the use of chips from planing (clean+dry)
wood or a similar material, more coarse than sawdust and either soaked in
water overnight or sterilized shortly with steam (allow to drip off a few
hours), instead of freshly chopped branches, is highly recommended. A lot of
these small, clean wood-chips can be used to feed, cover and spread your
outdoor bed. Mycelial growth produces CO2 and is stimulated by higher
concentration of CO2. Us glass jars for sterilizing grain media and large
plastic bags with only a few tiny holes for growing wood-spawn, and pack
these into boxes.
...
Outdoor culture:
Once more: SEVERAL WEEKS OF COLD WEATHER (0-10C) ARE REQUIRED FOR FRUITING.
...
Water:
If your bed is well drained, so that it cannot be over-watered, it can easily
be watered automatically . PCY-AO likes very wet conditions, and often grows
happily with water standing (literally) on their caps for days (at 0-10C).
PCY-AO often has a very high content of water: one batch, harvested after a
week of rain, contained only 5.5% dry matter.

JOHNNY APPLESEED ON PSILOCYBE CYANESCENS
Psilocybe cyanescens is indeed the best variety of mushrooms for home
cultivation, as reported in the Fall '95 ER. They are up to seven times as
potent as Psilocybe cubensis and can be grown outdoors in all temperate
climates with no need for sterile techniques.
...
They grow on fresh chips of Alder, Maple and Fir. I have used fresh Alder
(Alnus spp.) brush chipped in the Spring. I mix jars of spawn into garbage
bags of fresh woodchips and lay one foot deep beds of the inoculated chips
under bushes were they will receive permanent shade. They need to be
reasonable damp during the year and covering the chip piles with several
inches of leaves insures this.

It takes two years to produce a harvest in my northern climate (near Canadian
border). After the first year, the wood chips will be completely white with
the developing mycelium. At the end of the second growing season, late in the
Autum (November), they will fruit. Many times I have harvested my main crop
in the first snows, they seem to like cold temperatures to fruit.

Each bed will give only one good fruiting season. Therefore one needs to
start new beds every year. Take a shovelful of the white chips from the one-
year-old beds to inoculate fresh wood chips. This is a very low-tech and safe
way to grow powerful mushrooms almost everywhere.




Definitely read the rest, I just cut out the parts I want to have at hand.

While I love the idea of something that lives in my lawn under trees and bushes and fruits when the winter comes, I'm looking more for a strain that I can fruit within a couple of months, planted under some garden vegetables.  Gonna keep reading, but will also probably hit up a store for some woodchips to try out.  I wonder if Azurescens plays well with King Stropharia, a popular garden path edible mushroom I was thinking about cultivating.

Quote:
psylosymonreturns in this thread from 2013 says:
cultivation of P auzrescen is exactly the same as P cyanescen .Its also the same s Stropharia rugosoannulata. so anything you read for thats species works for azzies or other aggressive woodloving psilocybes like P allenii or P ovoid.



So they'd probably compete for nutrients, although they have a different fruiting cycle, so maybe worth a shot, but probably best to keep them in separate beds.

Looks like azurescens is tough to grow indoors, really mostly suited to outdoors.  Very few threads with successful indoor grows, best one is under mushroom info by abert Psilocybe azurescens indoor cultivation
agar=>wheat grain=>soaked wood chips=>1.5 months to colonize => cased with composted needles, foliage, sticks from the woods all chopped up uniform=>casing colonized, placed under wet vermiculite and refrigerated with ambient light through clear refrigerator door =>daily aired it out and humidified it => added fluorescent lamp 16hrs day=>mushrooms appear, 28 days later harvested. 
second grow added the light as soon as it was refrigerated and put it in a warmer part of the fridge 46-53F had fruits 2-3 weeks sooner

Smallish fruiting compared to bulk spawn of cubes, but impressive to get fruits within about 3 months from start compared to 2 years in the older documentation above with outdoor methods.
Interestingly, I searched for abert in the forums and his threads are about a cultivated Phallus Hadriani egg.  There's an awesome/weird/disgusting timelapse video of it growing here.  But doesn't talk about azurescens again.  Just a talented mycologist doing amazing things somewhere in Russia, and after 11/01/22, he hasn't been seen on the forums.

Well now I'm tired just thinking about doing this as a massive outdoor grow project, but I do have a little wine fridge that could serve as an azurescens grow chamber using the mysterious abert TEK. 

OK, I was going to do some agar work, but I'm too sleepy. Night y'all!

Extras: Filter Print Post Top
Re: New Hitchhiker Learns Not to Panic [Re: ArkaNaut]
    #28797251 -

Composed the evening of 6/1/24
Next Steps:
  • Try again to melt my chunky agar and pour some more plates
  • Inoculate 5 of the ten plates with each strain, sliding the inoculation loop through to spread out the LC and leave behind any sneaky hidden contam
  • Pluck some white mycelium from my trich'd out box and plate it on BRF agar and a PF Puck, just to see what happens.  Probably do that outside before I dump the MF in the compost. 
  • What the heck, might as well pick out the whitest looking granule from my bacteria soup GT bag and pop that on agar too.


Quote:
I peeked in my B+ bag.  It's all white and foamy, no pins, plenty of moisture on the sides.  :shrug:




Quote:
My B+ shoebox is still looking acceptable.  Keep reaching for the spraybottle and then slapping my hand.  Leave it alone.




Quote:
APE is looking better than the B+.  Hard to make it out but one of the primordia is mushroom shaped, and an eentsy bit taller each day.




Quote:
A mere 24 hours after inoculation, I'm already seeing myc on all of my PF pucks.  Hard to see through the glass. Azzie and Tamp are there, but faint.

Albino MVP                      Azurescens                        Tampanensis                    Avery's Albino                    B+



Edited by ArkaNaut (06/02/24 01:40 PM)

Extras: Filter Print Post Top
Re: New Hitchhiker Learns Not to Panic [Re: ArkaNaut]
    #28797258 -

Mein Gott im Himmel!
Es gibt einige Pins!



-please note, I only speak German ironically, or as punctuation, with the aid of Google translate

Since I'll be propagating these via cloning, they're going to need names I can put on Agar plates etc.

Henceforth, APE1, which was inoculated via Vogon Tek, shall be referred to as Vogon Albino Penis Envy, "VA"

This B+ shall in turn be known as Vogon B+, "VB"

I'll be adopting RogerRabbit's naming system described here

The dirty mycelium grow strains will be named "Dirty APE" - DA, and "Dirty Teacher" - DT.  The mother plates currently have "TrAPE" for "Trich APE" and "BacGT" for "Bacterial GT", but will be relabeled.

Edited by ArkaNaut (06/02/24 01:30 PM)

Extras: Filter Print Post Top
Re: New Hitchhiker Learns Not to Panic [Re: ArkaNaut]
    #28797275 -

My side project of attempting to rescue mycelium from heavily contaminated shoeboxes has begun.

Source Material

Golden Teacher                Alb PE(APE)

The three dark spots on the APE are the sample locations I chose. 
One I scraped the surface, the second I dug a little deeper, the third I scooped. 
I did not use the SAB, mainly out of concern for contaminating the SAB, but these samples are already screwed and covered in bacteria out of the gate. 
I did flame (and blacken) the tweezers before each transfer. 
I have an alcohol lamp but haven't set it up yet. 
Will be using that for all future transfers under a SAB. 
Below are the completed plates. 
I'll cut out whatever looks the most like mycelium and transplant to another BRF Agar plate.



After sampling and final pictures taken, the contents were dumped with great reverence into the compost pile.  It needed it badly, since we had thrown in a bit too much fly food and it was looking like a horror movie, desperately needed some more carbon.  I noted that there does seem to be thriving trich in the compost, so I'm glad it has found a useful thing to do, and presumably will contribute to some disease resistant tomatoes someday.

It was "cased" with a hydrated brick of coir, both to rehydrate the mycelium in hopes of giving it a place to outrun the trich and other contams, and also to cover up the fly babies and their disgusting nursery.  Coir is a great source of carbon for a compost pile, with a carbon:nitrogen ratio of 100:1.  I tend to also roughly tear up and throw in chunks of cardboard when the compost is too stinky, since cardboard has a C:N ratio of 300:1.  Coir helps hold it all together.

Someday, maybe, we'll have some actives poking up amongst the turnips and radishes.  It will be a glorious day.  Until then, back to the lab!

Edited by ArkaNaut (06/02/24 01:39 PM)

Extras: Filter Print Post Top
Glass no like temp change [Re: ArkaNaut]
    #28797765 -

So bit of a d’oh moment.

Heated my half empty bottle of solid agar in the microwave.  Proud of myself bc I got it all back to liquid.  I have a water bath next my workstation that I can bring to any particular temp.  It was at room temp.  For some reason I thought a borosilicate jar full of near boiling agar under pressure wouldn’t mind taking a dip in a room temp pool.  Crack!



Right, I knew about that.  Nuts.  Still the glass was intact, so I set up my SAB and poured the superheated agar into some plates.  It’s all practice so it’s fine, they’ll just have tons of condensation.

Now I have the other, unused, full of solid agar bottle in the water bath, gently coming up to 190F.  Last time I did this it didn’t melt, seems it does truly need to come to a boil.  So after the water hits 190 and it sits for a little while, I’ll pop it in the micro for a few seconds at a time, gently shake, and bring it back to the waterbath, still at 190, then dial the temp down to 120 and let it be for a good long while before trying again to pour.  I only have four empty plates left right now. But I have a box of 500 on the way.  Guess I should order another 500ml bottle.

I’ve seen some people have success with regular quart grain jars, which I have some coming, so maybe I’ll try that out before buying another bottle. I’m saving the plastic cap and pour liner thing.  I’ve heard they have a tendency to break so having a backup seems like a good idea.

Extras: Filter Print Post Top
Re: Glass no like temp change [Re: ArkaNaut]
    #28797772 -

Damn I thought I was goin pretty hard as a noob, but you're really going balls to the wall. Keep Killin it!


--------------------
🅃 🄴 🄰 🄼    🄲 🄻 🄸 🄽 🄶 🅆 🅁 🄰 🄿

"The lips of wisdom are closed, except to the ears of understanding." - The Kyballion, Three Initiates

Advanced Research of the Chronological History of Artifical Intelligence X

Extras: Filter Print Post Top
Re: Glass no like temp change [Re: Herme5]
    #28797805 -

:laugh: I have to admit I was jealous that you got the noob of the week.  I didn’t even know that was a thing.  But then I got it the week after, so jealousy resolved.  Not normally a competitive guy, but I think you helped drive me to be extra committed, even if it was just to stick things out.

I’ve got one pf puck with some happy looking avery albino, just saw your grow log on that, nicely done! 

Impossibly, I have some pins on my long sleeping tubs, so I’ll finally have something exciting to post if all goes well :wink:

Keep up the good work!

Extras: Filter Print Post Top
Re: New Hitchhiker Learns Not to Panic [Re: AzureCultivator]
    #28798119 -

AzureCultivator said:
tHeMaGe said:
Keep your finger on the off button toward the end if you don't want a mess in your microwave. I also put the jar in a mic safe bowl. Once you clean up an agar explosion once, you'll never want to do it again :smile:



Holy shit



Avoided a major mishap not that long ago -had a media bottle full of bubbling hot agar crack in my hand mid-shake


Still poured those fucking plates though.
(Thankfully I was wearing thick ass neoprene coated gloves and got away scott free)


--------------------
For it is only Darkness that crafts one to thrive in the Light. As illumination of the soul, its brilliance unmatched.
               
                    🌕🌖🌗🌘🌑🌒🌓🌔🌕
  :sporedrop:Gymnopilus subspectibilis grow log:sporedrop:             
•89g fresh subspectibilis tea•
•Rapéh Crafters•
• Gift yourself a healthy dose of Self banning•

Extras: Filter Print Post Top
Re: New Hitchhiker Learns Not to Panic [Re: Soloist]
    #28798169 -

Soloist said:
AzureCultivator said:
tHeMaGe said:
Keep your finger on the off button toward the end if you don't want a mess in your microwave. I also put the jar in a mic safe bowl. Once you clean up an agar explosion once, you'll never want to do it again :smile:



Holy shit



Avoided a major mishap not that long ago -had a media bottle full of bubbling hot agar crack in my hand mid-shake


Still poured those fucking plates though.
(Thankfully I was wearing thick ass neoprene coated gloves and got away scott free)




Damn!  It’s a testament to how tough that glass is.  Mine had cracked almost completely around.  You’re damn right I poured the plates.  Toasted my fingertips through the heat resistant gloves, but that agar got on those plates!

Extras: Filter Print Post Top
Jump to top Pages: | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 |  [ show all ]

Shop: Mushroom-Hut Grow Bags   North Spore Injection Grain Bag   Unfolding Nature Unfolding Nature: Being in the Implicate Order   Original Sensible Seeds Autoflowering Cannabis Seeds   Myyco.com APE Liquid Culture For Sale   MagicBag.co All-In-One Bags That Don't Suck   Sporeworks.EU Spores for European Microscopy


Similar ThreadsPosterViewsRepliesLast post
* Shroomy 1's Leucistic Texans
( 1 2 3 4 all )
P.Menace 8,804 72 02/14/09 09:02 AM
by dead
* albino TC encouragement?
( 1 2 all )
tuna 5,000 32 08/03/04 03:11 AM
by tuna
* Looking leucistic
( 1 2 all )
FrankHorrigan 4,535 34 02/23/14 10:10 PM
by cronicr
* Adding humidity to shoebox during colonization
( 1 2 3 4 ... 10 11 all )
AcidicMind 4,993 218 10/31/24 11:38 AM
by AcidicMind
* PF albino questions mickywilliams2 1,945 2 07/15/05 12:44 PM
by scatmanrav
* A few questions about my PF Albino DrugFreeMan 2,904 17 09/16/06 08:15 PM
by the_chosen_one
* albino shrooms cheesenoonions 3,720 1 06/07/02 12:39 PM
by Lapius
* Albino cubie - potency? jimsuzo 2,938 9 11/21/03 01:05 PM
by angryshroom

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: DERRAYLD, Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, the_chosen_one, LogicaL Chaos, 13shrooms, hamloaf, cronicr, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a
4,183 topic views. 11 members, 221 guests and 119 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2026 Mind Media. Some rights reserved.

Generated in 0.033 seconds spending 0.012 seconds on 16 queries.