First I have prepared mycelium on a sterilized wheat grain by inoculating it
from mycelium on agar. After the grain was colonized I inoculated alder chips
(not sterilized, soaked during 2-3 day in water) with this colonized grain.
The alder chips were dried chips, I bought them in a shop,
they are used for smoking fish etc.
Alder chips were colonized by the mycelium within 1.5 months, at a temperature
from 23-25°C. Then I placed the colonized alder chips in a plastic bottle
(first case) and put a casing layer on.
The casing layer consisted of composted needles, foliage, sticks
etc. from the woods. I removed large fragments so I was left with a homogeneous
mass. The casing layer was not sterilized.
After mycelium appeared on the surface of casing layer (temp 23-25°C), I
put a layer of wet vermiculite, and placed a bottle in a refrigerator (temp
6-8°C). (My refrigerator has a glass door and fluorescent lamp - such refrigerators
are used as a display for wine bottles etc.).
This is my refrigerator:
Regularly (two times per day) I aired and 1 time per 2 days humidified the bottle.
1.5 months later the first mushrooms have appeared.
The first mushrooms did not develop because of shortage of light (I used only
natural illumination, through the glass door), but mushrooms on the second flush
(that you can see in the pictures) developed normally. I used a fluorescent
lamp for 16 hours per day. From occurrence of mushrooms up to harvest 28 days
Development of the mushrooms in the bottle.
The second container was made in precisely the same way, only one month after
the first. What you see in the pictures is the first flush. Using a light from
the very beginning, and also having placed the container in the top part of
a refrigerator (where temperature is little bit higher: 8-12°C), the development
of the mushrooms could be reduced for 2-3 weeks. ( 2-3 weeks, because I noticed
mushrooms when they have appeared above a surface of the casing layer, from
this moment up to harvest have passed 2 weeks. But mushrooms needed some time
to appear above a surface... let's consider 3-7 days.).
The second container (substrate and casing layer were the same
as in the previous case).
And some more pics.
Two containers, placed on the top shelf, (where it is warmer). Second container
placed on a pan (in order to place it higher).
A plastic bottle with mushrooms inside (in full size).
And the harvest.