Bigworm said:

Yup no doubt!

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Kinoko314 said:

Best>Worst  -->  Red > Pink > Yellow = Orange

I'd take reds and pinks all day.

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RisnDevil said:

Ok, firstly, don’t judge me!

First time doing agar, and these are my first two dishes.




One was done with a swab, and the other with a hook scrape. (Yes, I gouged tf out of it, but like I said, literally my first time…)
Both were done Oct 23, so it’s been 12 days.

Now, the question(s): I think it’s time to transfer to new plates to further isolate from germs. In the two photos below, I made red circles to show which areas I think are viable for harvest for transfer, but I made smaller, blue circles for where I think it would be BEST to harvest.




And if I am correct, and those are viable and/or good options, when I put them on a new dish, do I just put the sample on top of the agar hand maybe lightly press it down)?

Thanks for any advice.

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ButtFace said:

Will this work to fruit these three shoeboxes in a 66 qt Sterlite tub or should I remove one of them?

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RisnDevil said:

Oh, I absolutely know it will take multiple transfers (I attempted to sterilize a ghetto hook and used a clean swab, so while I made some effort, I’m in no rush and would rather do multiple transfers to isolate, which I will now do with proper flame-and-alcohol sterilized tools in a still air box), but having never done this I was more verifying if those areas would continue to grow. I don’t know fully what I’m looking for/at to determine that.

I was looking at the growth and since I see clear “hair”/“fibers”, I thought it would be good for transfer. Is that what I should continue to look for? Is it more important to transfer a specific or larger size sample (even if it may be contaminated and have to be isolated again), or is a clean sample (almost no matter how small) more important when transferring?

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Pnin said:

QQ: How long can I leave plain nutrient broth sitting around after PCing?

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RisnDevil said:

Oh, I absolutely know it will take multiple transfers (I attempted to sterilize a ghetto hook and used a clean swab, so while I made some effort, I’m in no rush and would rather do multiple transfers to isolate, which I will now do with proper flame-and-alcohol sterilized tools in a still air box), but having never done this I was more verifying if those areas would continue to grow. I don’t know fully what I’m looking for/at to determine that.

I was looking at the growth and since I see clear “hair”/“fibers”, I thought it would be good for transfer. Is that what I should continue to look for? Is it more important to transfer a specific or larger size sample (even if it may be contaminated and have to be isolated again), or is a clean sample (almost no matter how small) more important when transferring?

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