Nerex said:

Putting, for those seconds, the donor's cover on a soaked tower with ISO 70% would be OK?

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RockinRobot said:

Nerex said:

Putting, for those seconds, the donor's cover on a soaked tower with ISO 70% would be OK?

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I never set the covers down. Learn to clamshell the top cover and hold both top and bottom plate with your non-dominant hand and use dominant hand to work the scalpel.

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Only in rare circumstances will I set lids down. Not typical for regular plate work. You can operate a stack of about 10 plates from bottom to top without needing to put lids anywhere. It's really pretty easy.


If you are going to set it down do so on a clean surface upside down, not on an iso towel.

tryptkaloids said:

Doesn't matter either way.

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CocaineBuffet said:

Will the likelihood you get increased contamination because you spent more time fumbling around with the inoculate across the surface of media? Yes.

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AspectOfTheCreator said:

QQ. Sorry for lack of pictures.

I notice a lot of my mushrooms, even in the good tubs of mine (ones putting out ~1.5 oz dry per qt spawn), have a high number of mushrooms growing horizontally along the surface of the substrate.

Why?

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Nerex said:

While transfering inside the SAB and after getting the little wedge, do you put the donor's petri dish cover back on it? I mean while you plan to keep taking more transfers from the donor.

When taking the little transfer, where do you put the cover for those seconds? On a paper towel soaked with ISO 70% is OK?

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OK, so I've read the comments of others, all of whom are likely a lot more experience and capable than I am, and they all make sense.  But...

I tape a big washer to both the base and the top of my plates before I start transfers. 

I attach the base to the rear side of the SAB using a magnet glued to the outside.  When I take the lid off, the pate is sideways on, reducing the likelihood of nasties falling in and making it easier (in my experience) to cut out a piece to transfer and pick it up with my scalpel.  Because the donor is immobilised by the magnet I can dig around without it moving.

In the meantime I use the washer on the lid to 'stick' it on a magnet attached to a curved bar, leaving the lid open side down and not touching anything.

From a good donor plate I'll make maybe 6 transfers (sometimes more) to recipients without ever closing the donor plate.  I know it breaks convention, but it works - I get maybe 5% failure rate on the recipients and the donor in every case so far stays 'clean' - I close it when I'm done and keep it so I can check a few days later.

I know it breaks with convention and may well be frowned upon by my peers, but

TheAtlantean said:

AspectOfTheCreator said:

QQ. Sorry for lack of pictures.

I notice a lot of my mushrooms, even in the good tubs of mine (ones putting out ~1.5 oz dry per qt spawn), have a high number of mushrooms growing horizontally along the surface of the substrate.

Why?

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Do you have a light that isn’t positioned above the grow? Maybe a window? Could be FAE related. Could be due to bacteria.

With/without pictures, there could be a few different reasons

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AspectOfTheCreator said:

TheAtlantean said:

AspectOfTheCreator said:

QQ. Sorry for lack of pictures.

I notice a lot of my mushrooms, even in the good tubs of mine (ones putting out ~1.5 oz dry per qt spawn), have a high number of mushrooms growing horizontally along the surface of the substrate.

Why?

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Do you have a light that isn’t positioned above the grow? Maybe a window? Could be FAE related. Could be due to bacteria.

With/without pictures, there could be a few different reasons

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Yea. The light comes from a window. The shrooms always grow in that direction. Just wasnt sure if it should be so severe

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DarwinGrayman said:

The melting point for psilocybin seams to be very high, like 280C/536F. So high temp doesn't seam that dangerous. (Mature assumption here)

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