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Shrimps
Traveler

Registered: 10/13/22
Posts: 2,501
Loc: Under the sea

Re: Ask Quick Questions, Get Quick Answers

2
#28508989 - 10/18/23 07:22 AM (3 years, 27 days ago)

I like to sterilize my stuff in the heart of mount doom

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Tiamo
Trust in LITFA

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Loc: Amsterdam

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Re: Ask Quick Questions, Get Quick Answers

[Re: perro]
#28508993 - 10/18/23 07:26 AM (3 years, 27 days ago)

perro said:

I this ok to flame sterilize scalpels/srynges, I think I need to step up my lighter game. I don't want an alcohol lamp.

That's what I use. It locks well, but sometimes goes out when turned to too low a setting so I have to run it a tiny bit higher than I'd like to.

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Edited by Tiamo (10/18/23 07:37 AM)

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Vast218
Learning

Registered: 04/24/22
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Re: Ask Quick Questions, Get Quick Answers

[Re: Tiamo]
#28509016 - 10/18/23 08:16 AM (3 years, 27 days ago)

Are all plastic lids for ball jars generally ok to PC? Or do I need to specifically buy the “autoclavable” ones sold at myco stores?

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PBJ710
Strangler

Registered: 07/05/19
Posts: 3,301
Last seen: 6 months, 3 days

Re: Ask Quick Questions, Get Quick Answers

[Re: Vast218]
#28509017 - 10/18/23 08:19 AM (3 years, 27 days ago)

Most are fine to use - which specific ones are you looking at?

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huey.bluey
((int(*)())0)();

Registered: 01/16/19
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Loc: Mississippi River
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Re: Ask Quick Questions, Get Quick Answers

[Re: PBJ710]
#28509023 - 10/18/23 08:30 AM (3 years, 27 days ago)

I was in a rush to get some melmac and I got some grade S bacteria here

... you think I could just eat it? wait its bacteria

Edited by huey.bluey (10/18/23 08:33 AM)

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tryptkaloids
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Re: Ask Quick Questions, Get Quick Answers

[Re: Vast218]
#28509026 - 10/18/23 08:36 AM (3 years, 27 days ago)

Vast218 said:

Are all plastic lids for ball jars generally ok to PC? Or do I need to specifically buy the “autoclavable” ones sold at myco stores?

The cheaper ones that huey just posted might warp if you put them on tight

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huey.bluey
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Registered: 01/16/19
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Re: Ask Quick Questions, Get Quick Answers

[Re: tryptkaloids]
#28509029 - 10/18/23 08:38 AM (3 years, 27 days ago)

tryptkaloids said:

Vast218 said:

Are all plastic lids for ball jars generally ok to PC? Or do I need to specifically buy the “autoclavable” ones sold at myco stores?

The cheaper ones that huey just posted might warp if you put them on tight

I have silicon rings + full sfd's on my lids with 1 x 1/4" hole. they seem snug to me, even after pc. one of my older lids was 10+ year old plastic lid and still pc'd fine. I used postal tyvek on the pint jars to test tyvek vs sfd. my quart and half gallon jars have the good sfd's

Edited by huey.bluey (10/18/23 08:40 AM)

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huey.bluey
((int(*)())0)();

Registered: 01/16/19
Posts: 2,459
Loc: Mississippi River
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Re: Ask Quick Questions, Get Quick Answers

[Re: huey.bluey]
#28509034 - 10/18/23 08:43 AM (3 years, 27 days ago)

dont use postal tyvek, the shroom grew through it some how. Im staring at the air barrier between the air, lid and outside of the tyvek and something is growin there. lol

I cant say I recommend eating this jar. It tastes really weird. almost like salsa. almost smells like a green pepper what the fuck

Edited by huey.bluey (10/18/23 08:59 AM)

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schmutzen
King of the side-pins

Registered: 12/03/02
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Re: Ask Quick Questions, Get Quick Answers

[Re: huey.bluey]
#28509055 - 10/18/23 08:59 AM (3 years, 27 days ago)

Making grain water agar, 10% GW and no LME...?

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Bustaboo
Calmer than you are

Registered: 07/17/23
Posts: 119
Last seen: 2 years, 3 months

Re: Ask Quick Questions, Get Quick Answers

[Re: schmutzen]
#28509069 - 10/18/23 09:08 AM (3 years, 27 days ago)

schmutzen said:

Making grain water agar, 10% GW and no LME...?

I made plates with 100% grain water and added 8g agar to 400mL solution. Worked great. Just can’t see through it much. If you have LME, I’d use that without any grain water. I like being able to see through the bottom.

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Smellyhobbit
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Re: Ask Quick Questions, Get Quick Answers

1
#28509074 - 10/18/23 09:13 AM (3 years, 27 days ago)

You don’t add LME to grain water agar, no.

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fiddle_head
Shroomery Whinery Vintner

Registered: 08/05/08
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Re: Ask Quick Questions, Get Quick Answers

[Re: stroopwafelman]
#28509088 - 10/18/23 09:21 AM (3 years, 27 days ago)

stroopwafelman said:

Anyone has an SFD recommendation that's sold in the EU (EU versions of amazon etc.)

Are you using a whole SFD or cutting? I’ve been using plastic lid 1/4” hole with sticker+tape; I’d highly recommend it.

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Pnin
Riz Gukgak

Registered: 07/18/23
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Re: Ask Quick Questions, Get Quick Answers

[Re: Smellyhobbit]
#28509090 - 10/18/23 09:22 AM (3 years, 27 days ago)

QQ: Is the only path from a print a syringe? Can I take the print to agar dry? I read BODs teks but they use a spore syringe with an inoculation loop.

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Smellyhobbit
Amateur Wizard

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Posts: 18,579
Loc: Booger Central
Last seen: 3 hours, 5 minutes

Re: Ask Quick Questions, Get Quick Answers

[Re: Pnin]
#28509091 - 10/18/23 09:23 AM (3 years, 27 days ago)

Pnin said:

QQ: Is the only path from a print a syringe? Can I take the print to agar dry? I read BODs teks but they use a spore syringe with an inoculation loop.

Of course man. Take your inoculation loop, rub it on your spore print, and streak your dish. Ezpz.

--------------------
A Love Letter to New Growers
The 7 Edicts of Mushroom Cultivation as Decreed by Smellyhobbit

:kodama:

:kodama:

Anyone can get a canopy. But can you get what you want?

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DERRAYLD
Constructus

Registered: 05/13/02
Posts: 15,028
Loc: South Africa
Last seen: 58 minutes, 31 seconds

Re: Ask Quick Questions, Get Quick Answers

[Re: Pnin]
#28509092 - 10/18/23 09:23 AM (3 years, 27 days ago)

Yes, prints can go straight to agar using a loupe, use a swab, use a scalpel, whatever you want to transfer and streak the spores.

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Pnin
Riz Gukgak

Registered: 07/18/23
Posts: 1,185
Last seen: 12 days, 15 hours

Re: Ask Quick Questions, Get Quick Answers

[Re: DERRAYLD]
#28509096 - 10/18/23 09:25 AM (3 years, 27 days ago)

Fuck yeah. Thanks. :agar:

:agar:

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huey.bluey
((int(*)())0)();

Registered: 01/16/19
Posts: 2,459
Loc: Mississippi River
Last seen: 1 year, 4 months

Re: Ask Quick Questions, Get Quick Answers

[Re: Pnin]

1
#28509102 - 10/18/23 09:28 AM (3 years, 27 days ago)

I yeeted all melmac from that batch into compost :salute:

:salute:

I dont feel high after eating it

Edited by huey.bluey (10/18/23 09:29 AM)

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schmutzen
King of the side-pins

Registered: 12/03/02
Posts: 23,660
Loc: On the railroad of sin
Last seen: 3 days, 21 hours

Re: Ask Quick Questions, Get Quick Answers

[Re: Smellyhobbit]

1
#28509108 - 10/18/23 09:33 AM (3 years, 27 days ago)

Smellyhobbit said:

You don’t add LME to grain water agar, no.

Thanks hobbit! :thumbup:

:thumbup:

--------------------

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SwabMarley
Twisted Metal Head

Registered: 12/07/20
Posts: 1,571
Loc: Drunken Stupor

Re: Ask Quick Questions, Get Quick Answers

[Re: Pnin]
#28509125 - 10/18/23 09:42 AM (3 years, 27 days ago)

Pnin said:

QQ: Is the only path from a print a syringe? Can I take the print to agar dry? I read BODs teks but they use a spore syringe with an inoculation loop.

I like to use a sterile swab. Works great.

Swab Love!

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tree frog
sapphic love muffin

Registered: 03/31/14
Posts: 7,049

Re: Ask Quick Questions, Get Quick Answers

[Re: SwabMarley]
#28509161 - 10/18/23 10:15 AM (3 years, 27 days ago)

Is coffee still being used for sclerotia? I know this came up recently for cubes.

Did anyone other than RR do side by sides for stones with/without coffee soak?

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Shop:

Liquid Cultures

Spores for European Microscopy

Injection Grain Bag

Certified Organic All-In-One Grow Bags by Magic Bag

Isolated Cubensis Liquid Culture For Sale

Mushrooms, Mycology and Psychedelics > Mushroom Cultivation

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