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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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Re: Ask Quick Questions, Get Quick Answers [Re: AspectOfTheCreator]
    #28489677 -

How long do you let your agar plates sit after pouring to see if any contams pop up? I can finally get back to work and need to pour plates first!

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Re: Ask Quick Questions, Get Quick Answers [Re: dr.snuggles] * 3
    #28489681 -

I’m a rebel; I use them once room temp. If you’re going to wait, wait a week.


--------------------
A Love Letter to New Growers
The 7 Edicts of Mushroom Cultivation as Decreed by Smellyhobbit

:kodama:


Anyone can get a canopy. But can you get what you want?

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Re: Ask Quick Questions, Get Quick Answers [Re: Nichrome]
    #28489691 -

Quick question: when everybody is talking about ratios (spawn to substrate) ... is it about weight or volume?

I caught myself calculating my a** off, switching between weight, volume, metric units, imperial units and so on and try to simplify sime things...

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Re: Ask Quick Questions, Get Quick Answers [Re: Speecher] * 1
    #28489693 -

Volume

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Re: Ask Quick Questions, Get Quick Answers [Re: Speecher]
    #28489695 -

Speecher said:
Quick question: when everybody is talking about ratios (spawn to substrate) ... is it about weight or volume?

I caught myself calculating my a** off, switching between weight, volume, metric units, imperial units and so on and try to simplify sime things...


Volume

IVE BEEN SNIPED


--------------------
A Love Letter to New Growers
The 7 Edicts of Mushroom Cultivation as Decreed by Smellyhobbit

:kodama:


Anyone can get a canopy. But can you get what you want?

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Re: Ask Quick Questions, Get Quick Answers [Re: Speecher]
    #28489697 -

Volume, I use the mass of dry coir and an expansion ratio to volume which finds the expanded volume of coir in my coir tek. spawn:sub ratios are always in vol

Edited by huey.bluey (10/02/23 09:35 AM)

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Re: Ask Quick Questions, Get Quick Answers [Re: huey.bluey] * 1
    #28489703 -



Can I use this for grain spawn?


--------------------


An hour and a half later, I was beginning to feel definite effects
Subjective Effect Index: comprehensive map of all potential psychoactive experiences

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Re: Ask Quick Questions, Get Quick Answers [Re: Mycodemus]
    #28489724 -

look like grains to me :thumbup:

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Re: Ask Quick Questions, Get Quick Answers [Re: voltik] * 1
    #28489808 -

voltik said:
What kind of contam is this? It's shown on both my failed attempts when spawning to coir in a shoebox, and it always shows up around the 2nd week mark. This one is darker, but the first time it had a more orangeish color which made it look like it was oxidizing even though that just sounds wrong.





Looks bacterial. Waaaaaaaaay bacterial. And most like some other type of mold.


The dark spots you see look like metabolic discharge.

It happens when there is an overgrowth of bacteria in a sub and when initial spawn is not clean.

I would start with agar until you are positive you have clean mycelium.


--------------------
Astra said:

“Peace River has a gentle personality, yet TTBVI hit me like strong LSD with a twist of medical grade meth. Not for the faint of heart.”

Team Panaeolus

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Re: Ask Quick Questions, Get Quick Answers [Re: TheAtlantean] * 1
    #28489810 -

I have some agar plates that are overgrown with tendrils growing up the sides of the walls. is that a sign of bacteria? Its not growing on nutrition but the condensation stuck on the wall. kind like voltik's box minus metabolites and mold.


This is a T1 tidal wave and I don't know what its doing, I marked it as contaminated and moved ahead with other plates of the culture.
I can't really see into it except the top.

Edited by huey.bluey (10/02/23 11:30 AM)

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Re: Ask Quick Questions, Get Quick Answers [Re: huey.bluey]
    #28489816 -

Hello all!

I have just acquired a bunch of PE LC syringes. :grin: My plan is to use one for an attempt to inoculate to grain jars using a variation of Broke Boi Tek that I've formerly done using spores. Later when I am not such a broke boi anymore and get my hands on a decent PC and put together a laminar flow hood, I will attempt to cultivate the rest of the syringes in a more sterile environment.

That said, should I place the LC syringes in a refrigerated storage now or will they last a little over a month in room temp?


--------------------
:mushroom2:

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Re: Ask Quick Questions, Get Quick Answers [Re: huey.bluey]
    #28489817 -

huey.bluey said:
Volume, I use the mass of dry coir and an expansion ratio to volume which finds the expanded volume of coir in my coir tek. spawn:sub ratios are always in vol


What did you find was the expansion ratio?  I'm approximating 70-75 grams per quart, based on some of my own measurements.

I thought when I added some very fine verm to my coir that the total volume wouldn't go up by much, but I was wrong.  The volume of verm almost completely added to the total volume.

It seems like most people are doing ratios as myco-quarts:quarts.  Does anyone do it quarts:quarts instead?  I guess the most important thing is that everyone is on the same page, but I don't love the inaccuracy of myco-quarts.

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Re: Ask Quick Questions, Get Quick Answers [Re: Kinoko314]
    #28489821 -

I have no idea what a myco-quart is. I use liter / milliliter measures only. Verm does not expand.  0.08 expanded liters / 6.9 grams dry coir is what I measure but its approximately based on 6.9 grams which is very low. I'll be testing with higher masses here soon and updating my expansion ratio.

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Re: Ask Quick Questions, Get Quick Answers [Re: TheAtlantean]
    #28489825 -

TheAtlantean said:
voltik said:
What kind of contam is this? It's shown on both my failed attempts when spawning to coir in a shoebox, and it always shows up around the 2nd week mark. This one is darker, but the first time it had a more orangeish color which made it look like it was oxidizing even though that just sounds wrong.





Looks bacterial. Waaaaaaaaay bacterial. And most like some other type of mold.


The dark spots you see look like metabolic discharge.

It happens when there is an overgrowth of bacteria in a sub and when initial spawn is not clean.

I would start with agar until you are positive you have clean mycelium.


So is there any reason why it doesn't show until almost a month has passed? Jars took 12 days to colonize and looked clean, but they clearly weren't considering the picture of the shoebox. Then it took another 2 weeks until I could see those colored areas.
First time I tried colonizing jars and got contaminants I could see bacteria after a couple days on the grain, but these last 2 times I didn't, that's what's weirding me out.

And yes, I'm now in the process of cleaning some different varieties on agar. Will probably make a grow log post so it's easier for people to point out my mistakes considering it's the 2nd time I've failed when spawning.

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Re: Ask Quick Questions, Get Quick Answers [Re: huey.bluey] * 1
    #28489838 -

huey.bluey said:
I have some agar plates that are overgrown with tendrils growing up the sides of the walls. is that a sign of bacteria? Its not growing on nutrition but the condensation stuck on the wall. kind like voltik's box minus metabolites and mold.


This is a T1 tidal wave and I don't know what its doing, I marked it as contaminated and moved ahead with other plates of the culture.
I can't really see into it except the top.


Use some tweezers and pull it off the wall and stuff it into another plate.
In had something similar with my TW and it was one of my best colonizers tbh. Sent it to grain a little over a week ago and still going strong


--------------------





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Re: Ask Quick Questions, Get Quick Answers [Re: MicroMycelium]
    #28489931 -

Yea that culture looks aggressive, and that's a good thing


--------------------
"Remember, kids, the difference between science and screwing around is writing it down" -adam savage
Flowchart for Recommended plan of action.
Learn the tried and true way to grow mushrooms
Use the Damn search engine
After you know what you're doing, take a break 
Pick a book, Make some chips!
Josex said:Don't take the site seriously bro, ain't worth it.
 

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Re: Ask Quick Questions, Get Quick Answers [Re: tryptkaloids]
    #28489950 -

SO whats the consensus. If i got pins and my sub is dry. should i worry about misting?

Should we mist at all stages?

does misting really cause abortion?


--------------------

BEEFS ABSOLUTE INTRO TO GROWING, BEGINNERS CLICK HERE!!!

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Re: Ask Quick Questions, Get Quick Answers [Re: chikn fingaz] * 1
    #28489960 -

Spacecadet94 said:
SO whats the consensus. If i got pins and my sub is dry. should i worry about misting?

Should we mist at all stages?

does misting really cause abortion?


if your surface is dry, gently fine mist sparingly from above and restrict fae just a tiny bit.
edit:

if your sub is dry (pulling away from walls) then your grow is in god's hands..


--------------------
the observer is the observed
            j. krishnamurti


>The Hitchhiker‘s Guide to the Shroomery<

Edited by chris77 (10/02/23 02:45 PM)

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Re: Ask Quick Questions, Get Quick Answers [Re: chris77]
    #28489963 -

Mist only aborts exotics


--------------------
"Remember, kids, the difference between science and screwing around is writing it down" -adam savage
Flowchart for Recommended plan of action.
Learn the tried and true way to grow mushrooms
Use the Damn search engine
After you know what you're doing, take a break 
Pick a book, Make some chips!
Josex said:Don't take the site seriously bro, ain't worth it.
 

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Re: Ask Quick Questions, Get Quick Answers [Re: tryptkaloids] * 2
    #28489967 -

tryptkaloids said:
Mist only aborts exotics


but not semperviva/subtropicales.. it makes them thrive


--------------------
the observer is the observed
            j. krishnamurti


>The Hitchhiker‘s Guide to the Shroomery<

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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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