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Re: Ask Quick Questions, Get Quick Answers [Re: Smellyhobbit]
    #28483027 -

The shot glass is the move, one drop at a time is a pita. Every time i buy a syringe it's tempting to take some to grain because there's so damn much solution and it takes so little to get a culture going.


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"Remember, kids, the difference between science and screwing around is writing it down" -adam savage
Flowchart for Recommended plan of action.
Learn the tried and true way to grow mushrooms
Use the Damn search engine
After you know what you're doing, take a break 
Pick a book, Make some chips!
Josex said:Don't take the site seriously bro, ain't worth it.
 

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Re: Ask Quick Questions, Get Quick Answers [Re: tryptkaloids] * 1
    #28483032 -

Syringes for PF tek, prints for agar. If youโ€™ve got leftover syringes, gather them in a bundle and give them away.

Spores are free for members who meet requirements. I love our trusted vendors here, but really once youโ€™ve graduated from PF tek spore syringes arenโ€™t that useful.


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A Love Letter to New Growers
The 7 Edicts of Mushroom Cultivation as Decreed by Smellyhobbit

:kodama:


Anyone can get a canopy. But can you get what you want?

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Re: Ask Quick Questions, Get Quick Answers [Re: Smellyhobbit]
    #28483040 -

I know, I just prefer to get ape directly from the source. A couple other varieties caught my eye, but they were out of prints so I settled with an impulse buy lol. Partially driven by impatience :lol:


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"Remember, kids, the difference between science and screwing around is writing it down" -adam savage
Flowchart for Recommended plan of action.
Learn the tried and true way to grow mushrooms
Use the Damn search engine
After you know what you're doing, take a break 
Pick a book, Make some chips!
Josex said:Don't take the site seriously bro, ain't worth it.
 

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Re: Ask Quick Questions, Get Quick Answers [Re: tryptkaloids]
    #28483058 -

Two questions:

is it normal for stipes to be hollow halfway up or can I grow solid ones?

Instead of taking a spore print I can just use a sterile swab right? How long should I wait before streaking the swab to agar?


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๐Ÿ…ƒ ๐Ÿ„ด ๐Ÿ„ฐ ๐Ÿ„ผ    ๐Ÿ„ฒ ๐Ÿ„ป ๐Ÿ„ธ ๐Ÿ„ฝ ๐Ÿ„ถ ๐Ÿ…† ๐Ÿ… ๐Ÿ„ฐ ๐Ÿ„ฟ

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Re: Ask Quick Questions, Get Quick Answers [Re: Nichrome]
    #28483061 -

Stipes can be hollow or fully solid. Genetics play a role.

You can swab your fruits and take it directly to agar.


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๐Ÿ…ƒ ๐Ÿ„ด ๐Ÿ„ฐ ๐Ÿ„ผ    ๐Ÿ„ฒ ๐Ÿ„ป ๐Ÿ„ธ ๐Ÿ„ฝ ๐Ÿ„ถ ๐Ÿ…† ๐Ÿ… ๐Ÿ„ฐ ๐Ÿ„ฟ
TEAM SPREAD THE LOVE!
Smellyhobbit said:
Embarrassment and bashfulness are leeches on your ability to learn.

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Re: Ask Quick Questions, Get Quick Answers [Re: HILLBILLY OUTLAW]
    #28483078 -

HILLBILLY OUTLAW said:
Stipes can be hollow or fully solid. Genetics play a role.

You can swab your fruits and take it directly to agar.




Be sure to do it gently or you might be getting a clone from a gill fragment instead.


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Learn about breeding

C10โ€™s agar guide
Good surface conditions = Good pinsets
Read more, post less.
๐Ÿ…‚ ๐Ÿ„ฐ ๐Ÿ„ผ ๐Ÿ„ด  ๐Ÿ…ƒ ๐Ÿ„ด ๐Ÿ„ฐ ๐Ÿ„ผ
๐Ÿ…ƒ ๐Ÿ„ด ๐Ÿ„ฐ ๐Ÿ„ผ  ๐Ÿ„ฒ ๐Ÿ„ป ๐Ÿ„ธ ๐Ÿ„ฝ ๐Ÿ„ถ ๐Ÿ…† ๐Ÿ… ๐Ÿ„ฐ ๐Ÿ„ฟ

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Re: Ask Quick Questions, Get Quick Answers [Re: AyePlus]
    #28483233 -

AyePlus said:
HILLBILLY OUTLAW said:
Stipes can be hollow or fully solid. Genetics play a role.

You can swab your fruits and take it directly to agar.




Be sure to do it gently or you might be getting a clone from a gill fragment instead.



Thatโ€™s why I like to let my swabs sit for a week or so and dry out before streaking


--------------------
A Love Letter to New Growers
The 7 Edicts of Mushroom Cultivation as Decreed by Smellyhobbit

:kodama:


Anyone can get a canopy. But can you get what you want?

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Re: Ask Quick Questions, Get Quick Answers [Re: Smellyhobbit]
    #28483391 -

When I remove micropore tape for inoculation, do I need to cover the jar lid holes again after inoculating?

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Re: Ask Quick Questions, Get Quick Answers [Re: Kashakunaki]
    #28483401 -

If you're talking PF Cakes with a dry verm layer, you don't need the tape at all :thumbup:


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You're conscious population 2 stardust
----------------------
Nothing matters so everything does :sunny:

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Re: Ask Quick Questions, Get Quick Answers [Re: Kashakunaki]
    #28483403 -

Kashakunaki said:
I've got a two quick questions!

After inoculating my wild bird seed quart jars that have a layer of vermiculite at the top, the holes previously covered by micropore tape, do I need to recover the lid holes with more tape, foil, or anything?  I assume they stay open for air, but I'm not sure.

Second, I see a lot of TEKs saying to use about 0.25 CC per injection sight with the commonly recommended number of injection sites being four, so 1 CC per jar.  Is there a particular reason for that or is that just a minimum?  I'm planning to do 1 CC per injection site - 4 CCs per jar - simply because that's how much I have and the rest will go bad if I don't use it, so if the only issue is it being a bit wasteful I'm fine with that.

Thank you!




I think you're mixing teks.  A grain jar shouldn't have a dry verm layer. 

Spores to grain is a bad practice that will give you contamination.  pf-tek can handle it, but not grain jars. 

Using too much liquid when inoculating can mess up the water content and encourage bacteria to grow. 

Technically you don't need to MP tape the holes if you have a dry verm layer, but since that shouldn't be there, you should keep it filtered/taped.

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Re: Ask Quick Questions, Get Quick Answers [Re: Kinoko314]
    #28483406 -

:whathesaid:


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You're conscious population 2 stardust
----------------------
Nothing matters so everything does :sunny:

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Re: Ask Quick Questions, Get Quick Answers [Re: MicroMycelium] * 2
    #28483459 -

MicroMycelium said:
These are my first fully colonized jars with oats, they colonized much different than my typical WBS. They look okay?



Also is this contamination or stress from something else?
Itโ€™s from a clean clone plate and looked great at first at 100%
But I forgot about it for a week on my shelf and developed a slight yellow discharge and shrank
I shook it when I noticed and it recovered to this state, shook it again recovered to its shrunken state again. Shook a third time, it recovered to this and metabolites(?) are still present.

Iโ€™ve read an even colonized bottom, recovery, and no rejected grains mean itโ€™s good to go, as well as that leaving one too long can cause shrinkage and heat can cause metabolites to developโ€ฆ which I did all of, but still looks sketchy.



Oats look fine to me.  Shoebox the millet just in case.


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๐Ÿ…‚๐Ÿ„ธ๐Ÿ„ถ๐Ÿ„ผ๐Ÿ„ฐ


Helpful Threads
stonesun
Mycoloradoโ€™s Tamp Fruit Project
Tampanensis Grow with Mycolorado
bw86's Jalisco Grow

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Re: Ask Quick Questions, Get Quick Answers [Re: Mycolorado]
    #28483496 -

QQ
What is the best way to prevent condensation on glass plates?


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"Let yourself be silently drawn by the strange pull of what you really love. It will not lead you astray." โ€“ Divan-e-Shams

How to upload Photos

The Hitchhiker's Guide to the Shroomery

Fruiting Cubes


Golden Teachers

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Re: Ask Quick Questions, Get Quick Answers [Re: milkboy]
    #28483626 -

Huskies said:
Do cut fresh shrooms loose potency in the fridge?



milkboy said:
Only in theory



Can anyone expand on this?

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Re: Ask Quick Questions, Get Quick Answers [Re: stockw] * 1
    #28483642 -

They will go bad and make you sick before not making you trip. The loss of potency is negligible

Really curious what hes cutting his mushrooms with, fent?

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Re: Ask Quick Questions, Get Quick Answers [Re: stockw]
    #28483649 -

stockw said:
Huskies said:
Do cut fresh shrooms loose potency in the fridge?



milkboy said:
Only in theory



Can anyone expand on this?




They turn black and rot if you don't dehydrated them. I'm sure you could still trip on them but you would probably get sick. Back in the day before people properly dried mushrooms vomiting was an expected part of eating them. If they are not properly dehydrated they are mushy and gross to eat.

There is really no reason not to dehydrated them in a dehydrator, they are like 20 dollars at goodwill. It's such a simple step I don't understand why people try to skip it after going though the rest of the grow process.


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๐ŸŒพ๐Ÿ’ฎ๐Ÿซ˜๐Ÿฅ–

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Re: Ask Quick Questions, Get Quick Answers [Re: hellofresh] * 1
    #28483684 -

i agree with that post but ill add you can keep fresh shrooms in the fridge for about a week in a paper bag and still be potent.

one time i found some months old shrooms i lost in the fridge. boiled them up and tripped well enough but they werent to sharp but hey they were months old in the fridge:shrug:

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Re: Ask Quick Questions, Get Quick Answers [Re: Proposition 122]
    #28483695 -

Pour when bottle is just near luke warm to the touch


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Re: Ask Quick Questions, Get Quick Answers [Re: AspectOfTheCreator]
    #28483725 -

AspectOfTheCreator said:
Pour when bottle is just near luke warm to the touch



I have been pouring at 120.
???


--------------------
"Let yourself be silently drawn by the strange pull of what you really love. It will not lead you astray." โ€“ Divan-e-Shams

How to upload Photos

The Hitchhiker's Guide to the Shroomery

Fruiting Cubes


Golden Teachers

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Re: Ask Quick Questions, Get Quick Answers [Re: Proposition 122]
    #28483737 -

I had two jars of a Clone, made from 2 plates from 1 sample.

I split them. None have contamed.

From Jar A, they look like this before I shook them just now. Surprisingly good on a no-prep oat tek.



Now, all from Jar B have mycelium that looks like this. It comes straight from the g2g grains.



Is that for sure a contam? Or a weird expression of mycelium? Shook them, will find out soon either way I suppose!


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I call them Huskies cause they Mush!


Huskies' Grimoire
The Official Wild Cubensis Thread
Easy Instant Pot Agar
Let's Talk Cowtek

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Shop: Sporeworks.EU Spores for European Microscopy   Original Sensible Seeds Autoflowering Cannabis Seeds   North Spore Injection Grain Bag   MagicBag.co Certified Organic All-In-One Grow Bags by Magic Bag   Myyco.com APE Liquid Culture For Sale   Mushroom-Hut Liquid Cultures


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