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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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Re: Ask Quick Questions, Get Quick Answers [Re: Stipe-n Cap]
    #28475423 -

Finally, the mush gods have sent me a sign to start some tubs!

At the end of the day… Sure, they are mushrooms, and I can and will grow more. But I will probably never make as many learning mistakes, or work as hard as I did growing these particular ones :lol:

On a serious note, would you just consider them fucked at this point?

Also, is this a common issue with vac bags? Seems wild to me that they can remain compressed/seemingly airtight while also allowing moisture transmission


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🅃 🄴 🄰 🄼  🄲 🅁 🅄 🄽 🄲 🄷 🅆 🅁 🄰 🄿

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Re: Ask Quick Questions, Get Quick Answers [Re: Nonagon]
    #28475525 -

I have a tub that has very grey mycelium, i didnt upload a picture because its does no justice and the lighting is off. i gave it a dab with a qtip and its coming back clean. i got beads of water on the surface so what gives?


Sorry im a noob open to any and all comments


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BEEFS ABSOLUTE INTRO TO GROWING, BEGINNERS CLICK HERE!!!

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Re: Ask Quick Questions, Get Quick Answers [Re: RoscoeReturns] * 1
    #28475537 -

RoscoeReturns said:
Proposition 122 said:
Wide mouth, 1 quart, 32oz, 946ml.
I am talking dry oats.
You?


I think that’s where the confusion comes in. A quart of spawn~a pound of spawn. I fit almost one pound of HYDRATED wheat in my quart jars. No way you get a pound of dry grain plus water in a quart jar. The pound=quart is a handy estimation for comparing with guys that use bags for grain spawn.


Got it! I think you're right. Thanks :thumbup:
Sorry about any confusion.


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"Let yourself be silently drawn by the strange pull of what you really love. It will not lead you astray." – Divan-e-Shams

How to upload Photos

The Hitchhiker's Guide to the Shroomery

Fruiting Cubes


Golden Teachers

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Re: Ask Quick Questions, Get Quick Answers [Re: Proposition 122]
    #28475610 -

Proposition 122 said:
Wide mouth, 1 quart, 32oz, 946ml.
I am talking dry oats.
You?


Same. Never used dry oats. The measurements are post hydration. Maybe that’s why you can’t make them fit. They should get denser with water

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Re: Ask Quick Questions, Get Quick Answers [Re: Zav3r]
    #28475633 -

Is shining a light under agar a good tool to ASSIST with identifiying contamination. from my studies i found pure healthy mycelium will be 100% transparent and see through on a plate when a light a flashed from underneath.

while mycelium with contamination will show DARK PATCHES AND BLOTCHES wherever contamn is present.

Anyone use this trick before. Noob here please go easy


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BEEFS ABSOLUTE INTRO TO GROWING, BEGINNERS CLICK HERE!!!

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Re: Ask Quick Questions, Get Quick Answers [Re: chikn fingaz]
    #28475636 -

Is it normal for agar inoculated grain to be giving off moisture?

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Re: Ask Quick Questions, Get Quick Answers [Re: lifescientist]
    #28475649 -

lifescientist said:
Is it normal for agar inoculated grain to be giving off moisture?


Some slight condensation is ok, but beyond that it’s a sign of contamination. I have a few jars that fit that description now that I’ll be spawning to shoeboxes soon.

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Re: Ask Quick Questions, Get Quick Answers [Re: chikn fingaz]
    #28475653 -

Spacecadet94 said:
Is shining a light under agar a good tool to ASSIST with identifiying contamination. from my studies i found pure healthy mycelium will be 100% transparent and see through on a plate when a light a flashed from underneath.

while mycelium with contamination will show DARK PATCHES AND BLOTCHES wherever contamn is present.

Anyone use this trick before. Noob here please go easy


That’s a good way to see contams, but the easiest way to get advice is to post a pic and explain if you germinated the plate, transferred or cloned.

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Re: Ask Quick Questions, Get Quick Answers [Re: Bustaboo]
    #28475661 -

I have one jar that is like <1/5 full with oats that looks clearly contaminated with B. subtilis I think. The other ones seem to be giving off small amounts of moisture but it's hard to tell.

I cannot figure out where or how I am getting the contam. I read somewhere that jars that do not have enough stuff in them need longer to pasteurize. Also I'm doing 1.5 hours I think or whatever was in Bod's guide.

This is sad and frustrating

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Re: Ask Quick Questions, Get Quick Answers [Re: lifescientist]
    #28475673 -

2 hours for jars. Sometimes 2.5 or 3hrs if larger than quart jars. Adjust PSI for altitude.

You've got this! One step at a time.


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Thanks to everyone for sharing what they know :smile:. 165F for 24hrs.

Better Holes Tek (Use a Step Drill Bit)
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Re: Ask Quick Questions, Get Quick Answers [Re: xVadisx]
    #28475676 -

Wouldn't B. subtilis show before inoculation? Or can it be introduced while the agar is being cut?

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Re: Ask Quick Questions, Get Quick Answers [Re: xVadisx]
    #28475681 -

My SAB didn’t have air still enough. I had a draft or something screwing my stuff up. Or my arm holes weren’t big enough or I was too hot and making the air warmer and swirl. Who knows. Instead of figuring that out, I got a fan filter unit that’s been awesome so far.

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Re: Ask Quick Questions, Get Quick Answers [Re: Bustaboo] * 2
    #28475717 -

Bustaboo said:
My SAB didn’t have air still enough. I had a draft or something screwing my stuff up. Or my arm holes weren’t big enough or I was too hot and making the air warmer and swirl. Who knows. Instead of figuring that out, I got a fan filter unit that’s been awesome so far.


It drives me nuts that instead of learning proper procedure people just drop a bunch of money on a tool to bandaid their bad technique.

Don't get me wrong, id love to have a ffu, and maybe this is just jealousy talking. But being able to work in a sab is an important thing to know for making prints and transferring moldy plates.


--------------------
"Remember, kids, the difference between science and screwing around is writing it down" -adam savage
Flowchart for Recommended plan of action.
Learn the tried and true way to grow mushrooms
Use the Damn search engine
After you know what you're doing, take a break 
Pick a book, Make some chips!
Josex said:Don't take the site seriously bro, ain't worth it.
 

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Re: Ask Quick Questions, Get Quick Answers [Re: tryptkaloids]
    #28475721 -

The table my SAB sits on is at a less than ideal height for me. It's waist high. I don't have room in my cramped apartment for a larger table. I do the best I can, but it is super uncomfortable and it hurts like a *****. My arms unfortunately have moved the box, but I try to be deliberate with movement and it is not easy.

I want to do a filter tek or whatever but I don't have the space or skills for a HEPA filter box

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Re: Ask Quick Questions, Get Quick Answers [Re: lifescientist]
    #28475722 -

lifescientist said:
Wouldn't B. subtilis show before inoculation? Or can it be introduced while the agar is being cut?


If anything is in your jar then either it was introduced during inoculation or you have a faulty filter.

Usually improper procedure.


--------------------
"Remember, kids, the difference between science and screwing around is writing it down" -adam savage
Flowchart for Recommended plan of action.
Learn the tried and true way to grow mushrooms
Use the Damn search engine
After you know what you're doing, take a break 
Pick a book, Make some chips!
Josex said:Don't take the site seriously bro, ain't worth it.
 

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Re: Ask Quick Questions, Get Quick Answers [Re: tryptkaloids] * 1
    #28475761 -

tryptkaloids said:
Bustaboo said:
My SAB didn’t have air still enough. I had a draft or something screwing my stuff up. Or my arm holes weren’t big enough or I was too hot and making the air warmer and swirl. Who knows. Instead of figuring that out, I got a fan filter unit that’s been awesome so far.


It drives me nuts that instead of learning proper procedure people just drop a bunch of money on a tool to bandaid their bad technique.

Don't get me wrong, id love to have a ffu, this is just jealousy talking. But being able to work in a sab is an important thing to know for making prints and transferring moldy plates.



FTFY

Because you'd never use a calculator or spell check or password save features? 🤔 despite being taught how to use them properly?


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Yeah, yeah.

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Re: Ask Quick Questions, Get Quick Answers [Re: NFLProof]
    #28475816 -

Tbf 'proper procedure' is using a ffu in my opinion.

Using a SAB is like using a handsaw instead of a power saw

When I was doing lots of 3D cad work a few years ago it was always the old dudes complaining and saying you youngsters should start on the drawing board to understand how it all works....

Took me 20 minutes to finish a design that took them 5 days to complete.

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Re: Ask Quick Questions, Get Quick Answers [Re: DERRAYLD] * 2
    #28475873 -

The thing is, bad sterile technique is bad sterile technique in an SAB or in front of a hepa. A flow hood CAN hide that a bit, but you’ll still get your share of contams. An SAB will show you every mistake you make. Learning to be proficient in an SAB makes you much better whether you stay in an SAB or move to a FFU IMO.


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Method for Small Scale Casing Pasteurization

How much really fits in a 00 capsule?

Micro and Macro Mushroom Photography

Can you freeze mycelium?


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Re: Ask Quick Questions, Get Quick Answers [Re: RoscoeReturns]
    #28475881 -

I'm not hating on a SAB but the general rule of thumb is superior equipment intended for the application is more suited to the application and therefore enables you to learn your craft in more focused manner.

Telling people to use a SAB to improve their technique is cou ter productive if you have a hood.
Rather hone your skill/craft using a flowhood because the route to success will be shorter.

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Re: Ask Quick Questions, Get Quick Answers [Re: psilocyboss]
    #28475915 -

psilocyboss said:
I've got some PF cakes that started fruiting just over a month ago, looks like they're done now. Is there any chance i could break them up and put into some coir for more fruits?


?


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off on an adventure, exploring entheogens🌱🌌

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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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