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tryptkaloids
Learner

Registered: 02/08/15
Posts: 16,590
Loc: Exact Center
Last seen: 7 hours, 57 minutes

Re: Ask Quick Questions, Get Quick Answers

[Re: Kinoko314]

1
#28463323 - 09/09/23 01:55 PM (3 years, 2 months ago)

Things I need to do today.
Inoculate plates with spore solution
Inoculate grains with agar
Mix substrate
Spawn
Make peat casing
Pasteurize it
Pour plates

What's the most time effective way to get all that done? What would your workflow be?

Side question.

My last order of plates came with a bag of soft plastic pipettes, I was thinking of using one to swab my plates, but after I open them can they be re sterilized as I use them? Or are they useless in this hobby?

--------------------
"Remember, kids, the difference between science and screwing around is writing it down" -adam savage
Flowchart for Recommended plan of action.
Learn the tried and true way to grow mushrooms
Use the Damn search engine
After you know what you're doing, take a break
Pick a book, Make some chips!
Josex said:Don't take the site seriously bro, ain't worth it.

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Culliganman360
Stranger

Registered: 06/06/19
Posts: 854

Re: Ask Quick Questions, Get Quick Answers

[Re: tryptkaloids]
#28463344 - 09/09/23 02:36 PM (3 years, 2 months ago)

Personally i would start with pouring plates. While them hoes cool down i would start doing all my other shit prolly next would b mixing sub cuz faster crop lol .
But
I just ordered sum plates from the zon and they came with some pipets also lol first time thought i was a lil weird and was wondering the same if they would hold up in pc. Try it out broham

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tryptkaloids
Learner

Registered: 02/08/15
Posts: 16,590
Loc: Exact Center
Last seen: 7 hours, 57 minutes

Re: Ask Quick Questions, Get Quick Answers

[Re: Culliganman360]
#28463358 - 09/09/23 03:04 PM (3 years, 2 months ago)

Dealing with pastrurizing peat is gonna take longer than mixing sub, so I think I'm gonna mix peat while waiting for my agar to cook and cool, then hopefully get it started cooking while I pour, might even be able to do some other sab work meanwhile.

--------------------
"Remember, kids, the difference between science and screwing around is writing it down" -adam savage
Flowchart for Recommended plan of action.
Learn the tried and true way to grow mushrooms
Use the Damn search engine
After you know what you're doing, take a break
Pick a book, Make some chips!
Josex said:Don't take the site seriously bro, ain't worth it.

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Mycolorado
Hobbyist

Registered: 07/23/16
Posts: 8,692
Loc: Interdimensional Bootcamp

Re: Ask Quick Questions, Get Quick Answers

[Re: tryptkaloids]
#28463365 - 09/09/23 03:21 PM (3 years, 2 months ago)

You ever sterilize 500 ml worth of media ingredients in 300 ml of water? :facepalm:

:facepalm:

--------------------

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tryptkaloids
Learner

Registered: 02/08/15
Posts: 16,590
Loc: Exact Center
Last seen: 7 hours, 57 minutes

Re: Ask Quick Questions, Get Quick Answers

[Re: Mycolorado]
#28463369 - 09/09/23 03:26 PM (3 years, 2 months ago)

Lol, thick and rich, just how I like my ladies. :lol:

:lol:

I'd like to see the myc grown on that agar

--------------------
"Remember, kids, the difference between science and screwing around is writing it down" -adam savage
Flowchart for Recommended plan of action.
Learn the tried and true way to grow mushrooms
Use the Damn search engine
After you know what you're doing, take a break
Pick a book, Make some chips!
Josex said:Don't take the site seriously bro, ain't worth it.

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Nichrome
-675 kPa

Registered: 12/17/18
Posts: 8,963

Re: Ask Quick Questions, Get Quick Answers

[Re: Kinoko314]
#28463469 - 09/09/23 05:39 PM (3 years, 2 months ago)

Kinoko314 said:

So you're not going through a SHIP then, Nichrome?

No just open the lid and draw.

--------------------
Question everything, it's very responsive.

Culture Storage

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MicroMycelium
Curious Optimizer

Registered: 06/06/23
Posts: 461
Loc: Probably at work

Re: Ask Quick Questions, Get Quick Answers

[Re: Nichrome]
#28463478 - 09/09/23 05:45 PM (3 years, 2 months ago)

Nichrome said:

Kinoko314 said:

So you're not going through a SHIP then, Nichrome?

No just open the lid and draw.

What if we want to drink with the forbidden straw

--------------------

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Nichrome
-675 kPa

Registered: 12/17/18
Posts: 8,963

Re: Ask Quick Questions, Get Quick Answers

[Re: MicroMycelium]

1
#28463481 - 09/09/23 05:48 PM (3 years, 2 months ago)

If I saw, I'd say ha ha, 'specially if it was done south paw.

--------------------
Question everything, it's very responsive.

Culture Storage

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blackout

Registered: 07/17/00
Posts: 5,266
Last seen: 3 years, 12 days

Re: Ask Quick Questions, Get Quick Answers

[Re: Whyterye]
#28463487 - 09/09/23 05:54 PM (3 years, 2 months ago)

Whyterye said:

With LC how do you get all the liquid out? Do you use long needles?

I use a SHIP and no filter and jars with good lids, this means I can turn them upside down. I prefer bottles with a neck, these can be left to settle upside down and the myc typically settles in the neck, so I can insert a needle and pull out liquid thick with LC.

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MicroMycelium
Curious Optimizer

Registered: 06/06/23
Posts: 461
Loc: Probably at work

Re: Ask Quick Questions, Get Quick Answers

[Re: blackout]
#28463705 - 09/09/23 09:59 PM (3 years, 2 months ago)

My first well made(I hope) LC.
Do you guys think it’s ready to test on agar or should I wait a little longer?
Inoculated with a josex poke 12 days ago. Been sitting on my stir plate since and the contents have been growing extremely fast.
I let it sit off the stirrer for a few minutes before this photo.

--------------------

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Smellyhobbit
Amateur Wizard

Registered: 04/01/22
Posts: 18,580
Loc: Booger Central
Last seen: 3 hours, 47 minutes

Re: Ask Quick Questions, Get Quick Answers

[Re: MicroMycelium]

1
#28463715 - 09/09/23 10:09 PM (3 years, 2 months ago)

Test that sumbitch

--------------------
A Love Letter to New Growers
The 7 Edicts of Mushroom Cultivation as Decreed by Smellyhobbit

:kodama:

:kodama:

Anyone can get a canopy. But can you get what you want?

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CocaineBuffet
Stranger

Registered: 08/30/19
Posts: 4,505
Last seen: 1 day, 13 hours

Re: Ask Quick Questions, Get Quick Answers

[Re: Smellyhobbit]
#28463824 - 09/09/23 11:48 PM (3 years, 2 months ago)

:whathesaid:

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MicroMycelium
Curious Optimizer

Registered: 06/06/23
Posts: 461
Loc: Probably at work

Re: Ask Quick Questions, Get Quick Answers

[Re: Smellyhobbit]
#28463939 - 09/10/23 02:46 AM (3 years, 2 months ago)

Smellyhobbit said:

Test that sumbitch

We shall see
:ifyoucanawe:

:ifyoucanawe:

--------------------

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Luckystriketony
Stranger

Registered: 09/08/23
Posts: 8
Last seen: 2 years, 9 months

Re: Ask Quick Questions, Get Quick Answers

[Re: MicroMycelium]
#28464005 - 09/10/23 07:43 AM (3 years, 2 months ago)

2 weeks after transfer to coco/verm, 1:2,5 spawn to substrate, looking good?

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DERRAYLD
Constructus

Registered: 05/13/02
Posts: 15,028
Loc: South Africa
Last seen: 2 hours, 26 minutes

Re: Ask Quick Questions, Get Quick Answers

[Re: Luckystriketony]
#28464007 - 09/10/23 07:50 AM (3 years, 2 months ago)

Take a photo from the top with the lid open.

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Argyreia
Grafting cacti is awesome

Registered: 11/19/14
Posts: 1,103
Loc: France
Last seen: 1 year, 4 months

Re: Ask Quick Questions, Get Quick Answers

[Re: Luckystriketony]
#28464009 - 09/10/23 07:53 AM (3 years, 2 months ago)

I'm planning on sterilizing honey/water solution in a glass shot, drop some spores from a print directly out of the pressure cooker and suck the solution into sterile syringes.
Will this work or am I getting into trouble ?

As a side question, when I will squirt out the liquid culture, can I let a bit of mycelium in the syringe, flame the needle and suck sterile honey/water to make it grow back ?

Edited by Argyreia (09/10/23 07:54 AM)

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Luckystriketony
Stranger

Registered: 09/08/23
Posts: 8
Last seen: 2 years, 9 months

Re: Ask Quick Questions, Get Quick Answers

[Re: Luckystriketony]
#28464010 - 09/10/23 07:58 AM (3 years, 2 months ago)

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RoscoeReturns
We’ve outstayed our welcome

Registered: 02/13/18
Posts: 3,129
Loc: State of Confusion
Last seen: 4 hours, 41 minutes

Re: Ask Quick Questions, Get Quick Answers

[Re: Luckystriketony]
#28464047 - 09/10/23 09:13 AM (3 years, 2 months ago)

Luckystriketony said:

Too dry. Looks matted. What size tote is it in, and how many holes, what size holes. Have you misted at all?

--------------------
Method for Small Scale Casing Pasteurization

How much really fits in a 00 capsule?

Micro and Macro Mushroom Photography

Can you freeze mycelium?

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RoscoeReturns
We’ve outstayed our welcome

Registered: 02/13/18
Posts: 3,129
Loc: State of Confusion
Last seen: 4 hours, 41 minutes

Re: Ask Quick Questions, Get Quick Answers

1
#28464048 - 09/10/23 09:15 AM (3 years, 2 months ago)

Argyreia said:

I'm planning on sterilizing honey/water solution in a glass shot, drop some spores from a print directly out of the pressure cooker and suck the solution into sterile syringes.
Will this work or am I getting into trouble ?

As a side question, when I will squirt out the liquid culture, can I let a bit of mycelium in the syringe, flame the needle and suck sterile honey/water to make it grow back ?

Spores are never sterile. Spores to LC, just like spores to grain, are very likely to bring in contaminant organisms.

--------------------
Method for Small Scale Casing Pasteurization

How much really fits in a 00 capsule?

Micro and Macro Mushroom Photography

Can you freeze mycelium?

Extras:

tryptkaloids
Learner

Registered: 02/08/15
Posts: 16,590
Loc: Exact Center
Last seen: 7 hours, 57 minutes

Re: Ask Quick Questions, Get Quick Answers

[Re: RoscoeReturns]
#28464080 - 09/10/23 10:02 AM (3 years, 2 months ago)

Work with agar if you want to make a clean lc. Otherwise it's a massive crapshoot that will likely waste your time

--------------------
"Remember, kids, the difference between science and screwing around is writing it down" -adam savage
Flowchart for Recommended plan of action.
Learn the tried and true way to grow mushrooms
Use the Damn search engine
After you know what you're doing, take a break
Pick a book, Make some chips!
Josex said:Don't take the site seriously bro, ain't worth it.

Extras:

Pages: < First | < Back | 1236 | 1237 | 1238 | 1239 | 1240 | 1241 | 1242 | 1243 | 1244 | 1245 | 1246 | 1247 | 1248 | 1249 | 1250 | 1251 | 1252 | 1253 | 1254 | 1255 | 1256 | Next > | Last >

Shop:

Bulk Cannabis Seeds

Golden Teacher Liquid Culture For Sale

Spores for European Microscopy

Unfolding Nature: Being in the Implicate Order

All-In-One Bags That Don't Suck

Liquid Cultures

Mushrooms, Mycology and Psychedelics > Mushroom Cultivation

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