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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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Re: AGAR ENVY! (Anything and All things agar!) [Re: Arctic_Fox] * 3
    #28387003 -

Arctic_Fox said:
Smellyhobbit said:
Yeah, it’s that stinkin live mycelium that grows on some plates but not the ones I had in the PC for 90 minutes! That has to be it.


Did you get around to performing the test again?


No we’ve been struggling at my place with my fiancé’s boob post surgery. But I’m just about ruffled enough to do it tonight and get my answers.


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A Love Letter to New Growers
The 7 Edicts of Mushroom Cultivation as Decreed by Smellyhobbit

:kodama:


Anyone can get a canopy. But can you get what you want?

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Smellyhobbit] * 3
    #28387015 -

You big dum dum
Me smart smart


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Help US help YOU TEK

2023 Dehydrator TEK

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Screwup] * 4
    #28387023 -

Screwup said:
You big dum dum
Me smart smart


No u
:smug:


--------------------
A Love Letter to New Growers
The 7 Edicts of Mushroom Cultivation as Decreed by Smellyhobbit

:kodama:


Anyone can get a canopy. But can you get what you want?

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Smellyhobbit] * 2
    #28387029 -

Smellyhobbit said:
Screwup said:
You big dum dum
Me smart smart


No u
:smug:


:bestcry:


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🅃 🄴 🄰 🄼    🄲 🄻 🄸 🄽 🄶 🅆 🅁 🄰 🄿


Help US help YOU TEK

2023 Dehydrator TEK

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Screwup] * 2
    #28387033 -

:irdumbtard:

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Screwup] * 1
    #28387034 -

Screwup said:
Smellyhobbit said:
Screwup said:
You big dum dum
Me smart smart


No u
:smug:


:bestcry:


I’m sorry bro I was just jealous. I shouldn’t have said that


--------------------
A Love Letter to New Growers
The 7 Edicts of Mushroom Cultivation as Decreed by Smellyhobbit

:kodama:


Anyone can get a canopy. But can you get what you want?

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Smellyhobbit] * 2
    #28387057 -

It’s okay we all say things we don’t mean from time to time. As long as you updoot my posts I’ll always come back 🥺




Two maketi plates…or maybe one nat and one maketi idk I’ve stopped caring. Both transferred same time.


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🅃 🄴 🄰 🄼    🄲 🄻 🄸 🄽 🄶 🅆 🅁 🄰 🄿


Help US help YOU TEK

2023 Dehydrator TEK

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Tormato]
    #28387271 -

Can someone tell me why water is forming central to the mycelium growth? (Center Plate)
I know it's not general condensation from over hydrated agar because it's only appeared on two plates and it would appear on every plate if it was just overhydrated.
All plates are stored the same with the same temps/conditions.
I thought these water beads only normally form when a tub is colonized and about to start fruiting :confused:


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Re: AGAR ENVY! (Anything and All things agar!) [Re: MicroMycelium]
    #28387410 -

That plate do be lookin quite sus. Could be metabolites from something that isn’t cubensis.


--------------------
A Love Letter to New Growers
The 7 Edicts of Mushroom Cultivation as Decreed by Smellyhobbit

:kodama:


Anyone can get a canopy. But can you get what you want?

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Re: AGAR ENVY! (Anything and All things agar!) [Re: MicroMycelium] * 1
    #28387445 -

MicroMycelium said:
Can someone tell me why water is forming central to the mycelium growth?


Living organisms produce heat while catabolizing substrate. Heat will produce condensation. This is why excessive condensation indicates bacterial infection, it's called microbial thermogenesis.

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Stipe-n Cap]
    #28387497 -

Stipe-n Cap said:
MicroMycelium said:
Can someone tell me why water is forming central to the mycelium growth?


Living organisms produce heat while catabolizing substrate. Heat will produce condensation. This is why excessive condensation indicates bacterial infection, it's called microbial thermogenesis.


So I should assume any plate with condensation is has a bacterial contaminant hitchhiking in the mycelium?
Would performing another transfer from the plate, and if the issue isn’t replicated on the next transfer, be a good measure to rule out bacterial contamination and assume it’s something else?
I’ve read that metabolic activity from the mycelium can produce these droplets as well. my agar is high-er nutrient than my previous plates so I was leaning toward that but you’re the pro and I’m the noob lol


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Edited by MicroMycelium (07/07/23 12:05 AM)

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Re: AGAR ENVY! (Anything and All things agar!) [Re: MicroMycelium] * 1
    #28387606 -

MicroMycelium said:
So I should assume any plate with condensation is has a bacterial contaminant hitchhiking in the mycelium?


I don't think so, because condensation can also occur because the temperature of the plate dropped below the dew point of the air inside it.


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Re: AGAR ENVY! (Anything and All things agar!) [Re: MicroMycelium]
    #28388427 -

Use discretion. Mycelium produces heat some small amount of heat as well. If your plate is infected there will be more to observe than just a bit of condensation.

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Re: AGAR ENVY! (Anything and All things agar!) [Re: MicroMycelium] * 1
    #28388430 -

MicroMycelium said:
So I should assume any plate with condensation is has a bacterial contaminant hitchhiking in the mycelium?


No, not at all.

In my experience 99% of the time it's what Arctic_Fox said. If I leave my window open overnight all my plates get condensated to shit. It clears up over time :tongue:


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How I grow mushrooms 🍄
How I do agar 🦠

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Throwaway]
    #28388438 -

Yep, like a cold can of beer.


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Helpful Threads
stonesun
Mycolorado’s Tamp Fruit Project
Tampanensis Grow with Mycolorado
bw86's Jalisco Grow

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Stipe-n Cap]
    #28388460 -

Stipe-n Cap said:
Use discretion. Mycelium produces heat some small amount of heat as well. If your plate is infected there will be more to observe than just a bit of condensation.


Ok drunky :vibin::rofl:

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Tormato] * 4
    #28388546 -

F5 to F6 tall x7x Flush 1 prints, 1 week on agar. I've been using flame sterilized scalpel (after cooling in agar) to slice a tiny bit of spore print through agar. I like the results. Grows like a caterpillar, gives more options of where to cut from. 



Will be transferring in a few days from plates 2, 3, and 4.

I've decided when I do a spore giveaway from this line at some point down the line, I'm going to send out spore prints, or sections of spore prints, instead of swabs. Prints taken with cookie trays seem cleaner to me, and I like working with even a fraction of a spore print much more than using a swab.

Here's how I took the very clean prints:






--------------------
--How to grow POPPIES.
--GROWING SHROOMS, FROM SPORES TO TRIPS
--x7x+ variety history.

Edited by nektar61 (07/08/23 05:15 AM)

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Re: AGAR ENVY! (Anything and All things agar!) [Re: nektar61]
    #28388636 -

Thanks a bunch for the previous replies...

Been having some trouble with my "alacabenzi" plates.
I put some wedges on rye, am I correct in assuming these are garbage?
There's clearly some healthy mycelium in there trying its best, but spawning would be a no-go... yeah?




Then we have these Treasure coasts..
I'm leaning towards these being okay, but wanted a second opinion.
Ropy rhizmorphic is easy to ID, but I'm still having trouble recognizing healthy mycelium on agar.
Some of these appear to be gray in the photos, but it's all white.


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Re: AGAR ENVY! (Anything and All things agar!) [Re: Miss Aya] * 1
    #28388653 -

Miss Aya said:
Thanks a bunch for the previous replies...

Been having some trouble with my "alacabenzi" plates.
I put some wedges on rye, am I correct in assuming these are garbage?
There's clearly some healthy mycelium in there trying its best, but spawning would be a no-go... yeah?




Then we have these Treasure coasts..
I'm leaning towards these being okay, but wanted a second opinion.
Ropy rhizmorphic is easy to ID, but I'm still having trouble recognizing healthy mycelium on agar.
Some of these appear to be gray in the photos, but it's all white.




Those jars appear to be bacterial and that last plate is riddled with bacteria.

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Re: AGAR ENVY! (Anything and All things agar!) [Re: buddzy]
    #28388667 -

Whoops, that last plate wasn't even supposed to be in the post, lol. I tossed that plate yesterday.

The other ones look good though? Thanks!

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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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