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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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Re: AGAR ENVY! (Anything and All things agar!) [Re: tholos]
    #28233030 -

tholos said:
Question on everyone's personal technique:

How long do you let a plate go before you take a transfer?


I always let my plates grow out to the edge of the agar, unless a contamination rears its ugly head. (In those cases, I always take my transfers as far away from the contamination site as possible, and as soon as possible.)

If my germ plate never shows any contamination, I send the germ plate to grain without ever transferring off it (unless I need to make more plates to knock up a specific number of jars - I usually do one plate per jar planned).

Peace & Light,

Seeker604


--------------------


Seeker’s LAGM 2023 Journal
Jake Oncid’s Tek for Panaeolus | Official Ps. Zapotecorum Thread | Moober's Notes on Growing Natalensis | Muad.Dweeb’s Breeding Resources

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Re: AGAR ENVY! (Anything and All things agar!) [Re: tholos]
    #28233081 -

Yeah I wait a little to see if it goes rizo. if I never see rizo then I’ll take a piece of leading edge. I had a germ plate that didn’t go ropey till it almost hit the edge of the plate.

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Re: AGAR ENVY! (Anything and All things agar!) [Re: CocaineBuffet]
    #28233189 -

Thanks for the advice. What are the visual markers for the certainty of contamination, the uneven & sporadic growth coupled with stalling? I'll be taking that transfer today, nice one.

Cocaine buffet sounds nice man have you been to one?

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Re: AGAR ENVY! (Anything and All things agar!) [Re: drVetker] * 1
    #28233193 -

I started having problems with condensation with my no pours after I switched to a different PC. I used to let them sit in the PC and cool overnight. I think what happens in this scenario is the glass and the water cool at different rates, causing condensation on the glass in the 100% humidity enviornment.

Try this. Either raise the jars or make the water level a bit more shallow so it doesn't make contact with the bottom of the jars. When the PC cycle is done, let the pressure release naturally. Then take the jars out while still hot. I use unmodified lids and leave them a bit loose while cooling and after inoculation. This solved my condensation woes.

Many say the condensation isn't an issue, but you have to think that if there's any contamination in there the water's going to spread it everywhere and contaminate everything as soon as a transfer is made. In other words you won't be able to outrun the contam. Also made germination impossible for me.


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Re: AGAR ENVY! (Anything and All things agar!) [Re: AspectOfTheCreator]
    #28233212 -

How is this LC test plate looking on day 2? I accidentally used too much as I do pours but would this be considered healthy? Or way too early to tell? Thanks <3


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Re: AGAR ENVY! (Anything and All things agar!) [Re: stockw] * 2
    #28233229 -

i dunno man

my lc test usually look like clean MS germ plates. that looks like bacterial syringe water. maybe its just the pic:shrug:


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:hypnotoad:
Making Liquid Culture :  Go insane with LC
Mushboy likes these teks : Making shroom tea
Dank Education

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Re: AGAR ENVY! (Anything and All things agar!) [Re: mushboy]
    #28233235 -

It's from this Z-strain plate which was originally looking great on the dish, really nice even rhizo growth.. but it keeps coming up bacterial or tomentose whenever I transfer it :/ This is from a Plate Pin also, idk if that'd make any difference.

Probably down to my technique I suppose.

Onwards & upwards eh.

Edited by stockw (03/17/23 06:55 AM)

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Re: AGAR ENVY! (Anything and All things agar!) [Re: tholos] * 1
    #28233270 -

tholos said:
Question on everyone's personal technique:

How long do you let a plate go before you take a transfer? Do you take it at the first site of a particular growth morphology that you want? Do you let the plate grow out a certain amount and then make your selection? Do you always transfer a leading edge?

I was checking back on a plate that I took some t1s from about 1 week ago and saw some super ropey rhizo growth that wasn't there when I took my original transfers, so it got me thinking


1) Inoculate plate with spores.
2) At 50% colonization, take transfers of fastest growth.
3) If transfer plate looks clean, allow to fully colonize and pin.
4) Transfer pins straight to grain.


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Re: AGAR ENVY! (Anything and All things agar!) [Re: San Pedro Girl] * 5
    #28233736 -

Accidental plate pin art. I put a 90% colonized plate in the refrigerator at 7°C to stall, but I guess its a cold weather clone.


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Re: AGAR ENVY! (Anything and All things agar!) [Re: San Pedro Girl]
    #28233777 -

Anybody use the RIT black fabric dye for agar? Pros and cons?

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Re: AGAR ENVY! (Anything and All things agar!) [Re: MonoVision]
    #28233800 -

MonoVision said:
Anybody use the RIT black fabric dye for agar? Pros and cons?


I use it only when I’m cleaning up a culture. It really does make a difference versus just straight water agar to save cultures from bacteria.

Never send Rit agar cultures directly to grain, though. You don’t want to consume the ink. I always return my cultures to nutrient agar that is safe to send to grain or LC.

Here’s my recipe; it’s enough for 15 plates:
500ml H2O
10g Rit Dye (Black liquid)
5g LME
10g Agar

Also, only use Rit black. My mentor has tried other colors, but for whatever reason, the black ink is best at slowing contamination.

🍄💙🍄


--------------------


Seeker’s LAGM 2023 Journal
Jake Oncid’s Tek for Panaeolus | Official Ps. Zapotecorum Thread | Moober's Notes on Growing Natalensis | Muad.Dweeb’s Breeding Resources

Edited by Seeker604 (03/17/23 01:40 PM)

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Seeker604] * 4
    #28233824 -

RWxMM plate pin clone




--------------------
LAGM2020

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Re: AGAR ENVY! (Anything and All things agar!) [Re: MonoVision] * 1
    #28233846 -

MonoVision said:
Anybody use the RIT black fabric dye for agar? Pros and cons?


I use a tiny scoop of activated charcoal in my agar to make them black and give nice contrast. I presume it's not nearly as bad to consume either.

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Seeker604] * 1
    #28233855 -

Seeker604 said:
MonoVision said:
Anybody use the RIT black fabric dye for agar? Pros and cons?


I use it only when I’m cleaning up a culture. It really does make a difference versus just straight water agar to save cultures from bacteria.

Never send Rit agar cultures directly to grain, though. You don’t want to consume the ink. I always return my cultures to nutrient agar that is safe to send to grain or LC.

Here’s my recipe; it’s enough for 15 plates:
500ml H2O
10g Rit Dye (Black liquid)
5g LME
10g Agar

Also, only use Rit black. My mentor has tried other colors, but for whatever reason, the black ink is best at slowing contamination.

🍄💙🍄


You can send rit dye agar straight to grain if you want. You won’t end up with anything toxic to you in your mushrooms.


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Re: AGAR ENVY! (Anything and All things agar!) [Re: Seeker604]
    #28233865 -

Seeker604 said:
MonoVision said:
Anybody use the RIT black fabric dye for agar? Pros and cons?


I use it only when I’m cleaning up a culture. It really does make a difference versus just straight water agar to save cultures from bacteria.

Never send Rit agar cultures directly to grain, though. You don’t want to consume the ink. I always return my cultures to nutrient agar that is safe to send to grain or LC.

Here’s my recipe; it’s enough for 15 plates:
500ml H2O
10g Rit Dye (Black liquid)
5g LME
10g Agar

Also, only use Rit black. My mentor has tried other colors, but for whatever reason, the black ink is best at slowing contamination.

🍄💙🍄


Wow thanks for the quick and thorough response. Gonna pour some up in the next couple days to see how it works for me :bow2:

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Re: AGAR ENVY! (Anything and All things agar!) [Re: MonoVision] * 1
    #28235162 -



T2 plate.  Took some 3rd transfers off another plate, going to put this on some grain in the meantime cause I'm bored.  You guys think this looks decent?

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Re: AGAR ENVY! (Anything and All things agar!) [Re: KeyMaker] * 1
    #28235227 -

Ps. niveotropicalis



Hypsizygus sp.



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Shrooms are always bluer on the other side...

Work hard, play harder, trip the hardest.

Simple Cloning TEK by Adas
Semi-Sterile TEK (SS-TEK)
Growing Panaeolus bisporus - The Low-Tech approach
Exotic Mycelia Surviving Winter - Creating IceBreaker Variants!
My First Panaeolus Cyanescens Grow!
Psilocybe azurescens photo compilation 2020
Azurescens in June!

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Adas]
    #28235228 -

Adas said:
Ps. niveotropicalis



Hypsizygus sp.




Niveotropicalis :creepylurker:


--------------------
:pm:Ask about free prints: Ps tampanesis, subtropicalis, cubensis(ESS) and Pan cyanescens:pm:
Balance in life is like running on ice.

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Re: AGAR ENVY! (Anything and All things agar!) [Re: SirPsycho]
    #28235517 -

What the fuck is going on with all these exotic species now!


--------------------
Everything in life is a trade-off.

All posts made by this account are purely satirical in nature.

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Eugene Gesuale] * 3
    #28235611 -

Eugene Gesuale said:
What the fuck is going on with all these exotic species now!


There's more to life than cubensis.  :smile: 

Plenty of other species give better trips than cubes and you don't need as much weight per dose/grow either.

Peace & Light,

Seeker604


--------------------


Seeker’s LAGM 2023 Journal
Jake Oncid’s Tek for Panaeolus | Official Ps. Zapotecorum Thread | Moober's Notes on Growing Natalensis | Muad.Dweeb’s Breeding Resources

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Shop: Sporeworks.EU Spores for European Microscopy   North Spore Injection Grain Bag   Original Sensible Seeds Bulk Cannabis Seeds   Mushroom-Hut Liquid Cultures   Myyco.com Isolated Cubensis Liquid Culture For Sale

Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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