DERRAYLD said:

You should make a yeast broth before hand and strain it prior to adding agar.
It comes out much clearer.

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shroomsurf said:

DERRAYLD said:

You should make a yeast broth before hand and strain it prior to adding agar.
It comes out much clearer.

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If my recipe is 7gm agar, 7gm ME then how would I make a yeast broth to add 1gm yeast broth to my 400ml media bottle and is it the Yeast that's making the agar trays have stuff in it?

Thanks,

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CowsPoopShrooms said:

shroomsurf said:

If my recipe is 7gm agar, 7gm ME then how would I make a yeast broth to add 1gm yeast broth to my 400ml media bottle and is it the Yeast that's making the agar trays have stuff in it?

Thanks,

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IMO, take your 400ml or lesser portion, heat the water add your NY and then filter threw a coffee filter, preferably 2x or even 3x. Personally, I would also add the ME to filter that also.

Good job on the cups you poured, but, yes, they are too thick. It won't do any harm, but it's just a waste of agar, time, effort. You should be able to get at least twice that many cups poured. When you go to do transfers you are going to need to shave off the portion you want or deal with columns of agar to place in the new cup.

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So the filtering can happen before or after adding the agar and do the ME and NY filtering together before in your example!?

CowsPoopShrooms said:

IMO, take your 400ml or lesser portion, heat the water add your NY and then filter threw a coffee filter, preferably 2x or even 3x. Personally, I would also add the ME to filter that also.

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No need to do anything crazy....

My plates are clear as a mofo.



No need for all that filter and pre boil fuss. Add powder, shake, pc...you don't even need to shake it's just an old habbit.

shroomsurf said:

CowsPoopShrooms said:

shroomsurf said:

If my recipe is 7gm agar, 7gm ME then how would I make a yeast broth to add 1gm yeast broth to my 400ml media bottle and is it the Yeast that's making the agar trays have stuff in it?

Thanks,

---

IMO, take your 400ml or lesser portion, heat the water add your NY and then filter threw a coffee filter, preferably 2x or even 3x. Personally, I would also add the ME to filter that also.

Good job on the cups you poured, but, yes, they are too thick. It won't do any harm, but it's just a waste of agar, time, effort. You should be able to get at least twice that many cups poured. When you go to do transfers you are going to need to shave off the portion you want or deal with columns of agar to place in the new cup.

---

So the filtering can happen before or after adding the agar and do the ME and NY filtering together before in your example!?

---

Smellyhobbit said:

I have only ever had sediment issues with adding nutritional yeast or making agar with flour. All malt extract based plates have been like blue glass.

The clarity of a plate isn’t super important though, is it? You may have trouble seeing the underside of your growth with BRF agar, but for the most part it’s no trouble to have cloudy agar.

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My second batch should be better with all this information you guys helped me out with and thinner cups ...lol