m0n0id said:

lol, I got stuck in Spanish too. Click the flag by the search box at the top of the Amazon page, you can switch your language back there. At least it worked for me.

Pretty sure HiMedia Yeast Extract Powder is what people are reporting as dissolving clear. Got some on order, we'll see...

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m0n0id said:

lol, I got stuck in Spanish too. Click the flag by the search box at the top of the Amazon page, you can switch your language back there. At least it worked for me.

Pretty sure HiMedia Yeast Extract Powder is what people are reporting as dissolving clear. Got some on order, we'll see...

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Marcus666 said:

I need to say a BIG thank you to the folks who suggested nutritional yeast in my mix.



Transfers from plates like this...



Made these...





That plate above is actual transfers from the original plate.



Please excuse the condensation... I'm pouring just below 50*C and using a mug of hot water on top, leaving them in front of the hood for a couple of hours, but still they get all misty and wet.

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SpaceBaby said:

milkboy said:

Lostkeys said:

Finally found a yeast that dissolves fully in water, it's yeast extract powder from HiMedia on Amazon.

Plates are 1.5% lme, 0.2% soya peptone, 0.2% yeast extract powder, 2% agar.

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This?

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yes.

didn't wait...ordered this:
https://www.amazon.com/-/es/Levadura-nutricional-polvo-onzas-paquete/dp/B0041U6KPY/ref=sr_1_1?__mk_es_US=%C3%85M%C3%85%C5%BD%C3%95%C3%91&keywords=yeast+powder+now+brand&qid=1638132057&qsid=140-6858529-6848824&sr=8-1&sres=B0041U6KPY%2CB07KNYN7F4%2CB07DP55WK3%2CB06Y1JPZ4F%2CB000VH3ZYU%2CB00028M47C%2CB07DDD56VF%2CB00014DZL6%2CB00NLR1PX0%2CB000MGR1N6%2CB00016XJM4%2CB000BD0S1W%2CB001F0R81I%2CB07S95VHM3%2CB0013OQOFW%2CB0013OXKHC%2CB07WNSJBKB%2CB00020HV1E%2CB07888Z95T%2CB0002JIXLM&srpt=HERBAL_SUPPLEMENT

i see it's not extract

somebody on here posted a spanish amazon link and now i cn't get out of it!
just ordered the himedia

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Ababyphoenix said:

Upgraded from my old media bottles i left behind when i moved that were only 500ml i do believe. Big boys

Petris here tomorrow

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I like media bottles for certain things. As far as agar prep i like to use a plastic autoclaveable pitcher with a spout and handle. Just my preference. I've also stopped using plastic plates in favor of reusable glass ones. Ever consider using a pitcher vs a media bottle?

D3_Myc said:

Ape 🦍

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LotKid said:

Ababyphoenix said:

Upgraded from my old media bottles i left behind when i moved that were only 500ml i do believe. Big boys

Petris here tomorrow

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I like media bottles for certain things. As far as agar prep i like to use a plastic autoclaveable pitcher with a spout and handle. Just my preference. I've also stopped using plastic plates in favor of reusable glass ones. Ever consider using a pitcher vs a media bottle?

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verum subsequentis said:

I don't PC mine to kill shit before cleaning. Just scoop that shit into the trash and wash them.

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verum subsequentis said:

I don't PC mine to kill shit before cleaning. Just scoop that shit into the trash and wash them.

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bigfootscreepyuncl said:

verum subsequentis said:

I don't PC mine to kill shit before cleaning. Just scoop that shit into the trash and wash them.

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I'm not gonna lie, I only get to do any cult work for a few months when my actual work season is over and once I winterize my equipment I'm lazy as FUCK lol. I like the idea of glass petris but that's way too much work for me. Yes, it's a ton of plastic waste but I do my best to make up for it in other places.

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Lostkeys said:

D3_Myc said:

Ape 🦍

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I've never seen a APE plate look so nice!

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Thanks, I got a couple different ape cultures on agar right now. This is definitely the nicest and very fast. I’m looking forward to seeing what it does in the long run. A plate from Last month already throwing chunker pins too.

Ababyphoenix said:

verum subsequentis said:

I don't PC mine to kill shit before cleaning. Just scoop that shit into the trash and wash them.

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Im a little bitch i guess. I figured i didnt want some of that shit in my air/space. I live in a tiny living space now so i idk if it would be worth it. Either way.

Time to get some glass petris.

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If it's super gross mix up soapy water in your sink and open it under water so the spores don't release in the air.


The only way I ever got clear clear plates was to boil, blend with an immersion blender and use cheese cloth. I got the opposite direction nowadays and use straight black plates like D3m shows



It is an amazing tool for visibility.

Faht I'd really love to see more writeups on how you get stuff done. Your methods are a little out there and interesting af

fahtster said:

It’s basically my oven bag cloning method but I just slide the iso’d plate into the bag, push all the air out and reseal.. then blow back up and do the transfers.. I dipped the agar pieces in 2 jars of sterile water before I put them in the grain jars.. sealed the jars and cut them out.. reclinged the plates


Faht

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I always love seein what you're up to Faht you think you'll follow the herd and pick up agar any time soon or stick to your awesome TEKs? You remind me of Boneyard Brewing - they got all their equipment out of a brewing scrapyard and crank out some of the best beer around with a cult following