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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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Re: AGAR ENVY! (Anything and All things agar!) [Re: m0n0id]
    #27560795 -

m0n0id said:
lol, I got stuck in Spanish too. Click the flag by the search box at the top of the Amazon page, you can switch your language back there. At least it worked for me.

Pretty sure HiMedia Yeast Extract Powder is what people are reporting as dissolving clear. Got some on order, we'll see...


I found it for $18 dollars for 100g. Kinda pricey though, but if it works as well as claimed, I'm in.


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My Grow Journal

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Paracosm] * 1
    #27560801 -



Upgraded from my old media bottles i left behind when i moved that were only 500ml i do believe. Big boys :snoopyes:

Petris here tomorrow


--------------------


:sporedrop::aum:Pho's MushiAventures::aum::sporedrop:
:heartpump:My '21 GLOG:heartpump:
:peace: LAGM 2022 :peace:


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Re: AGAR ENVY! (Anything and All things agar!) [Re: m0n0id]
    #27560980 -

m0n0id said:
lol, I got stuck in Spanish too. Click the flag by the search box at the top of the Amazon page, you can switch your language back there. At least it worked for me.

Pretty sure HiMedia Yeast Extract Powder is what people are reporting as dissolving clear. Got some on order, we'll see...


yah, me too. cancelled wrong, got right.
won't be able to try it unil the the 8th. biz trip tomorrow.


--------------------
SpaceBaby
SPACEBABY'S LAGM22 THREAD
MUSHBOY'S SHROOM TEA TEK

Me as a cube

Another Day's Work:


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Re: AGAR ENVY! (Anything and All things agar!) [Re: SpaceBaby] * 1
    #27561003 -

Marcus666 said:
I need to say a BIG thank you to the folks who suggested nutritional yeast in my mix.

:thumbsup:

Transfers from plates like this...



Made these...





That plate above is actual transfers from the original plate.

:bow2:

Please excuse the condensation... I'm pouring just below 50*C and using a mug of hot water on top, leaving them in front of the hood for a couple of hours, but still they get all misty and wet. :wow:


These are the exact same results I've had before and after adding yeast. I know people say it isn't necessary, but the proof is in the pudding.

SpaceBaby said:
milkboy said:
Lostkeys said:
Finally found a yeast that dissolves fully in water, it's yeast extract powder from HiMedia on Amazon.

Plates are 1.5% lme, 0.2% soya peptone, 0.2% yeast extract powder, 2% agar.



This?


yes.

didn't wait...ordered this:
https://www.amazon.com/-/es/Levadura-nutricional-polvo-onzas-paquete/dp/B0041U6KPY/ref=sr_1_1?__mk_es_US=%C3%85M%C3%85%C5%BD%C3%95%C3%91&keywords=yeast+powder+now+brand&qid=1638132057&qsid=140-6858529-6848824&sr=8-1&sres=B0041U6KPY%2CB07KNYN7F4%2CB07DP55WK3%2CB06Y1JPZ4F%2CB000VH3ZYU%2CB00028M47C%2CB07DDD56VF%2CB00014DZL6%2CB00NLR1PX0%2CB000MGR1N6%2CB00016XJM4%2CB000BD0S1W%2CB001F0R81I%2CB07S95VHM3%2CB0013OQOFW%2CB0013OXKHC%2CB07WNSJBKB%2CB00020HV1E%2CB07888Z95T%2CB0002JIXLM&srpt=HERBAL_SUPPLEMENT

i see it's not extract

somebody on here posted a spanish amazon link and now i cn't get out of it! :rolleyes:
just ordered the himedia


Here's a link to the extract I'm trying out. Results incoming when the plate grows out a bit.

Paracosm said:
m0n0id said:
lol, I got stuck in Spanish too. Click the flag by the search box at the top of the Amazon page, you can switch your language back there. At least it worked for me.

Pretty sure HiMedia Yeast Extract Powder is what people are reporting as dissolving clear. Got some on order, we'll see...


I found it for $18 dollars for 100g. Kinda pricey though, but if it works as well as claimed, I'm in.


I don't feel like it's that pricey when you can make like 2,500 plates with that one bottle.


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So heavy I fell through the earth...


Edited by Lostkeys (11/28/21 05:50 PM)

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Lostkeys]
    #27561059 -

[url=https://files.shroomery.org/files/21-47/814571808-20211128_192646.jpg]
[/url] Penicillium roqueforti, snagged from a chunk of blue chesse
Unidentified contam off a Ps semperviva spore plate.

I've started culturing my contams cause reasons. Found a nice member of the OMC to check these under a scope for me, I'll be sending out samples to them soon


--------------------
:pm:Ask about free prints: Ps tampanesis, subtropicalis, cubensis(ESS) and Pan cyanescens:pm:
Balance in life is like running on ice.

  🅑🅞🅣🅣🅛🅔 🅖🅐🅝🅖

"Mist your balls and fan your asshole" - Pandaskis, 2023

Edited by SirPsycho (11/28/21 05:32 PM)

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Ababyphoenix] * 1
    #27561130 -

Ababyphoenix said:


Upgraded from my old media bottles i left behind when i moved that were only 500ml i do believe. Big boys :snoopyes:

Petris here tomorrow




I like media bottles for certain things. As far as agar prep i like to use a plastic autoclaveable pitcher with a spout and handle. Just my preference. I've also stopped using plastic plates in favor of reusable glass ones. Ever consider using a pitcher vs a media bottle?


--------------------

:mushroom2: LotKid's PE Tips        :shpongle:    LotKid's Chocolates :mushroom2:
:dancingbear: :dancingbear:

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Re: AGAR ENVY! (Anything and All things agar!) [Re: SirPsycho] * 1
    #27561134 -

Ape 🦍



--------------------

D3’s Glog
D3's Agar TEK
Agar Porn Gallery
RW x MelMak Grow Along Thread

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Re: AGAR ENVY! (Anything and All things agar!) [Re: D3_Myc] * 1
    #27561148 -

I actually get to post in the agar thread! Lol. I was gifted a couple mak plates (bk and tp).  I used the ole oven bag to do some transfers to glc grain jars and they’re taking off already.. probably knock up some qts in a week or so.


Faht

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Re: AGAR ENVY! (Anything and All things agar!) [Re: D3_Myc]
    #27561172 -

D3_Myc said:
Ape 🦍




I've never seen a APE plate look so nice!


--------------------
So heavy I fell through the earth...


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Re: AGAR ENVY! (Anything and All things agar!) [Re: LotKid]
    #27561190 -

LotKid said:
Ababyphoenix said:


Upgraded from my old media bottles i left behind when i moved that were only 500ml i do believe. Big boys :snoopyes:

Petris here tomorrow




I like media bottles for certain things. As far as agar prep i like to use a plastic autoclaveable pitcher with a spout and handle. Just my preference. I've also stopped using plastic plates in favor of reusable glass ones. Ever consider using a pitcher vs a media bottle?


Ive not considered a pitcher no. You just foil over the top?

I kinda like the scientific vibe of the media bottles but a pitched for pouring a ton of plates at once would be nice i guess. Dont think ill go that big though.

As for glass petris ive considered im just lazy and also somewhat scared to imagine opening and cleaning out some of those dishes. Yes yes you pc them first to kill it off but thats a whole lot of ick and work.


--------------------


:sporedrop::aum:Pho's MushiAventures::aum::sporedrop:
:heartpump:My '21 GLOG:heartpump:
:peace: LAGM 2022 :peace:


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Re: AGAR ENVY! (Anything and All things agar!) [Re: Ababyphoenix] * 1
    #27561195 -

I don't PC mine to kill shit before cleaning. Just scoop that shit into the trash and wash them.


--------------------
Masters and slaves hunting clones        How not to isolate (it worked, but)


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Re: AGAR ENVY! (Anything and All things agar!) [Re: verum subsequentis]
    #27561200 -

verum subsequentis said:
I don't PC mine to kill shit before cleaning. Just scoop that shit into the trash and wash them.


Im a little bitch i guess. I figured i didnt want some of that shit in my air/space. I live in a tiny living space now so i idk if it would be worth it. Either way.

Time to get some glass petris.

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Ababyphoenix]
    #27561209 -

I'm not calling you a lil bitch at all. If I've got a real green forest in a glass dish, I may take it outside to dump it.


--------------------
Masters and slaves hunting clones        How not to isolate (it worked, but)


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Re: AGAR ENVY! (Anything and All things agar!) [Re: verum subsequentis] * 1
    #27561210 -

verum subsequentis said:
I don't PC mine to kill shit before cleaning. Just scoop that shit into the trash and wash them.


I'm not gonna lie, I only get to do any cult work for a few months when my actual work season is over and once I winterize my equipment I'm lazy as FUCK lol. I like the idea of glass petris but that's way too much work for me. Yes, it's a ton of plastic waste but I do my best to make up for it in other places.


--------------------



I :heart: 5318008


NOT a virgin!

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Re: AGAR ENVY! (Anything and All things agar!) [Re: bigfootscreepyuncl]
    #27561223 -

bigfootscreepyuncl said:
verum subsequentis said:
I don't PC mine to kill shit before cleaning. Just scoop that shit into the trash and wash them.


I'm not gonna lie, I only get to do any cult work for a few months when my actual work season is over and once I winterize my equipment I'm lazy as FUCK lol. I like the idea of glass petris but that's way too much work for me. Yes, it's a ton of plastic waste but I do my best to make up for it in other places.


Thats kind of how i feel right now. Starting all new germ plates im just setting myself up for a lot of dish cleaning time if i go glass right now.


--------------------


:sporedrop::aum:Pho's MushiAventures::aum::sporedrop:
:heartpump:My '21 GLOG:heartpump:
:peace: LAGM 2022 :peace:


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Re: AGAR ENVY! (Anything and All things agar!) [Re: Lostkeys] * 1
    #27561252 -

Lostkeys said:
D3_Myc said:
Ape 🦍




I've never seen a APE plate look so nice!



Thanks, I got a couple different ape cultures on agar right now. This is definitely the nicest and very fast. I’m looking forward to seeing what it does in the long run. A plate from Last month already throwing chunker pins too.



--------------------

D3’s Glog
D3's Agar TEK
Agar Porn Gallery
RW x MelMak Grow Along Thread

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Ababyphoenix]
    #27561285 -

The only way I ever got clear clear plates was to boil, blend with an immersion blender and use cheese cloth. I got the opposite direction nowadays and use straight black plates like D3m shows



It is an amazing tool for visibility.

Faht I'd really love to see more writeups on how you get stuff done. Your methods are a little out there and interesting af
Ababyphoenix said:
verum subsequentis said:
I don't PC mine to kill shit before cleaning. Just scoop that shit into the trash and wash them.


Im a little bitch i guess. I figured i didnt want some of that shit in my air/space. I live in a tiny living space now so i idk if it would be worth it. Either way.

Time to get some glass petris.


If it's super gross mix up soapy water in your sink and open it under water so the spores don't release in the air.


The only way I ever got clear clear plates was to boil, blend with an immersion blender and use cheese cloth. I got the opposite direction nowadays and use straight black plates like D3m shows



It is an amazing tool for visibility.

Faht I'd really love to see more writeups on how you get stuff done. Your methods are a little out there and interesting af

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Drboomer] * 1
    #27561310 -

It’s basically my oven bag cloning method but I just slide the iso’d plate into the bag, push all the air out and reseal.. then blow back up and do the transfers.. I dipped the agar pieces in 2 jars of sterile water before I put them in the grain jars.. sealed the jars and cut them out.. reclinged the plates


Faht

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Re: AGAR ENVY! (Anything and All things agar!) [Re: fahtster]
    #27561333 -

fahtster said:
It’s basically my oven bag cloning method but I just slide the iso’d plate into the bag, push all the air out and reseal.. then blow back up and do the transfers.. I dipped the agar pieces in 2 jars of sterile water before I put them in the grain jars.. sealed the jars and cut them out.. reclinged the plates


Faht


I always love seein what you're up to Faht :smile: you think you'll follow the herd and pick up agar any time soon or stick to your awesome TEKs? You remind me of Boneyard Brewing - they got all their equipment out of a brewing scrapyard and crank out some of the best beer around with a cult following


--------------------



I :heart: 5318008


NOT a virgin!

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Re: AGAR ENVY! (Anything and All things agar!) [Re: D3_Myc] * 1
    #27561357 -














Kinda getting burnt out. Not specifically with must cult just irl, dealing with shit.

Makes it hard to pour plates and make transfers when you're stressed n having dark thoughts.

The way she goes eh?

-

I'm struggling to get a uniform APE culture.

Even on T3 from swab, the growth is thick, dense, purely tomentose growth. It seems like there are a large amount of strains present.

How can I narrow down the genetics? I'm thinking either making a very small transfer or perhaps taking a transfer from the very edge of the growth where the mycelium is not visible to the naked eye

Another possibility is that I've been growing and transferring mold for the past 2 or so months.

I S2B'ed 2 x T1 APE jars today, hopefully to get a clone culture going.

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