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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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Re: AGAR ENVY! (Anything and All things agar!) [Re: jay.ach] * 1
    #27472838 -

Damn.. I should ask questions more often :lol:  thanks y’all.. interesting to hear what everyone does :thumbup:

Faht

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Re: AGAR ENVY! (Anything and All things agar!) [Re: fahtster]
    #27473043 -

Hi Shroomery more noob questions...  Been trying to get a good transfer off a TOC germination plate. I really need to tighten up my spore to agar game.



What do you think about the spots above the areas I highlighted? Thanks in advance and have a good weekend.


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Edited by Atomsplit (09/18/21 03:58 PM)

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Atomsplit] * 1
    #27473072 -

They definitely look like good areas to transfer from :thumbup:



Finally starting to get some decent looking and somewhat fast growing ovoid mycelium.

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Re: AGAR ENVY! (Anything and All things agar!) [Re: multifractal]
    #27473121 -

Thanks fractal. Isn't getting a clean culture such a great feeling?

I'm on T-6 on a regular cube lol. The yeast and lower lme seemed to help a bunch.


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Re: AGAR ENVY! (Anything and All things agar!) [Re: Atomsplit]
    #27473233 -


Took samples of this plate for a T2. Looks like I'm going in for more... So healthy


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So heavy I fell through the earth...


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Re: AGAR ENVY! (Anything and All things agar!) [Re: Lostkeys]
    #27473447 -

ive been having weird looking myc growth lately and im not sure if its high nutrients or a hidden contam or neither. ive been using the same recipe for years now though so i dont know why it would be growing weird unless it was contaminated or unhealthy or maybe i just took a transfer from a sector that is deciding to grow weird now

is this agar recipe particularly high in nutes? this is for small batches and i just scale up if needed
2.5g agar
2.5g potato flakes
1/2 cup water
1/2 tsp karo

i was thinking of dropping the karo on the next round of plates for lower nutes to try and clean up some strange looking plates

Edited by wonderousovoid (09/18/21 10:19 PM)

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Re: AGAR ENVY! (Anything and All things agar!) [Re: wonderousovoid]
    #27473474 -

wonderousovoid said:
ive been having weird looking myc growth lately and im not sure if its high nutrients or a hidden contam or neither. ive been using the same recipe for years now though so i dont know why it would be growing weird unless it was contaminated or unhealthy or maybe i just took a transfer from a sector that is deciding to grow weird now

is this agar recipe particularly high in nutes? this is for small batches and i just scale up if needed
2.5g agar
2.5g potato flakes
1/2 cup water
1/2 tsp karo

i was thinking of dropping the karo on the next round of plates for lower nutes to try and clean up some strange looking plates


Seems complicated. I like to keep it simple and just use LME and nutritional yeast.


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So heavy I fell through the earth...


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Re: AGAR ENVY! (Anything and All things agar!) [Re: Lostkeys]
    #27473485 -

Lostkeys said:
wonderousovoid said:
ive been having weird looking myc growth lately and im not sure if its high nutrients or a hidden contam or neither. ive been using the same recipe for years now though so i dont know why it would be growing weird unless it was contaminated or unhealthy or maybe i just took a transfer from a sector that is deciding to grow weird now

is this agar recipe particularly high in nutes? this is for small batches and i just scale up if needed
2.5g agar
2.5g potato flakes
1/2 cup water
1/2 tsp karo

i was thinking of dropping the karo on the next round of plates for lower nutes to try and clean up some strange looking plates


Seems complicated. I like to keep it simple and just use LME and nutritional yeast.


i mean its a run of the mill PDA recipe that i got from pastywhytes tek years ago. its always worked for me. i think he only specified a drop of karo though

i dont have any other ingredients for anything other than PDA agar at the moment as its all ive ever used.

just wondering if there would be enough nutes from just the potato flakes and not add the karo this time?

Edited by wonderousovoid (09/18/21 10:49 PM)

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Re: AGAR ENVY! (Anything and All things agar!) [Re: wonderousovoid]
    #27473723 -

bunch o stuff

input please

respond by pic #
1,2,4 all enigma lc streak. i can't read this shit for shit sure
the rest assorted transfers assorted ages

1


2


3


4


5


6


--------------------
SpaceBaby
SPACEBABY'S LAGM22 THREAD
MUSHBOY'S SHROOM TEA TEK

Me as a cube

Another Day's Work:


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Re: AGAR ENVY! (Anything and All things agar!) [Re: SpaceBaby]
    #27473801 -

O Mighty Agar Wizards of the Agar Envy Thread,

I humbly request that thou shareth thy knowledge of the Shroom.

I have but one simple question;

It’s totally cool to poke a hole in the side right?


These are my new pp5s, finally I got some that’s airtight. The black dot is where I want to make the hole for GE(using micropore). Any advice would be very much appreciated. I think it shouldn’t be a problem but I’d just like to double check with the pros.  :biggrin:

Cheers!

Edited by Biskop (09/19/21 03:38 AM)

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Biskop]
    #27473817 -

You could do but there’s more than enough air in that container for the mycelium to grow. Not needed



Tidalwave 4 T2

TAT T1

Cambodia Clone

Leucistic Colombian T1

Early growth on the T1s I know, but it’s my first ever of those varieties so I’m excited.


--------------------
Being pissed on does not make you a real man.

...OR DOES IT?

Edited by Guerrilla (09/19/21 03:54 AM)

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Guerrilla]
    #27473836 -

The holes are for the PC cycle, I’m pretty sure that it is necessary unless there’s something I’m missing?

EDIT: I’m doing no pours sorry, I should’ve mentioned that.

Nice pics though, is that last one contaminated? What are the tiny little weird thingys?

Edited by Biskop (09/19/21 04:15 AM)

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Biskop]
    #27473854 -

Oh yeah for no pours the hole will be cool. On the side is a good spot too, no need for foil.

No contam just nutritional yeast. I can't get it to dissolve, maybe I need to try powdered or a different brand.


--------------------
Being pissed on does not make you a real man.

...OR DOES IT?

Edited by Guerrilla (09/19/21 04:50 AM)

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Guerrilla]
    #27473860 -

It looks like you sprinkled it on top, but yeah now I see it’s part of the mix. Cool stuff.

@SpaceBaby those pics are really cool too, sorry for hijacking your post. I’m super jealous of you all with your crystal clear Petris!

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Biskop] * 2
    #27473865 -

I'd highly recommend nutritional yeast with LME for your agar recipe, if you're not already. You'll end up using it some point in the future and regret not using it sooner.


--------------------
Being pissed on does not make you a real man.

...OR DOES IT?

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Guerrilla]
    #27473891 -

Guerrilla said:
Oh yeah for no pours the hole will be cool. On the side is a good spot too, no need for foil.

No contam just nutritional yeast. I can't get it to dissolve, maybe I need to try powdered or a different brand.


I can't get it to dissolve either.

The yeast seems to float to the bottom of the media bottle after a PC cycle/while cooling down.

I have been rotating the bottle to redistribute the yeast while the agar cools.

Rotating the bottle seems to create air bubbles which stay on petri dishes after the agar has been poured.

Next run I'm going to leave the bottle to cool, without rotating it, to let the yeast sink to the bottom. Then I'll pour the agar and leave the yeast at the bottom of the bottle.

In theory, some yeast should still be in the poured dishes.

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Re: AGAR ENVY! (Anything and All things agar!) [Re: aNewBeginning]
    #27473919 -

I don't think it dissolves. You just need to grind it as finely as possible. I weigh it out into a glass bowl and use the back of a spoon, like a mortar and pestle.


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There are only 10 types of people in the world, those that understand binary and those that do not.


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Re: AGAR ENVY! (Anything and All things agar!) [Re: JXAllen]
    #27473983 -

BUMP
]
bunch o stuff

input please

respond by pic #
1,2,3 are all enigma lc streak. i can't read this shit for shit sure, but I ain't loving that color. Never stereaked an LC test before tho.
Is that darkness in the streaks contam or is that LC go to go?
It's thicker than snot:



1


2


3


THIS MORNING

4


5


--------------------
SpaceBaby
SPACEBABY'S LAGM22 THREAD
MUSHBOY'S SHROOM TEA TEK

Me as a cube

Another Day's Work:


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Re: AGAR ENVY! (Anything and All things agar!) [Re: SpaceBaby]
    #27473997 -

I don't know much about enigma, but that looks super contaminated...


--------------------
So heavy I fell through the earth...


Edited by Lostkeys (09/19/21 09:21 AM)

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Lostkeys]
    #27473999 -

Couple of recently colonized plates about to go in the mini fridge


--------------------
So heavy I fell through the earth...


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