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gone-pear-shaped
Stranger than fiction

Registered: 10/30/17
Posts: 822
Last seen: 1 year, 8 months

Re: AGAR ENVY! (Anything and All things agar!)

[Re: DcevLearn]
#27412514 - 08/03/21 01:06 AM (5 years, 2 months ago)

DcevLearn said:

I'm running a nutrient variable experiment right now...

Will post a thread if it ends up panning out.

That's great! Will you post the results even if you don't see any difference? That's an important result in itself.

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Ora
Stranger

Registered: 07/09/21
Posts: 734
Loc: in your heart
Last seen: 4 years, 10 months

Re: AGAR ENVY! (Anything and All things agar!)

[Re: gone-pear-shaped]
#27412941 - 08/03/21 01:36 PM (5 years, 2 months ago)

Sounds cool. I'd be interesting to get a comparison of different mediums and their effects.

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hogstomper
Strangler

Registered: 03/11/05
Posts: 100
Last seen: 4 years, 11 months

Re: AGAR ENVY! (Anything and All things agar!)

[Re: Ora]
#27412984 - 08/03/21 02:28 PM (5 years, 2 months ago)

I had some germ plates that I put a swab to about 6 weeks ago. Nothing every came up on them - can I use them for transfers or maybe to cool the scalpel? They look totally clean but I feel wary for some reason.

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no justice no peace. black trans lives matter.

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nektar61
Just a smear in the shale

Registered: 07/04/20
Posts: 4,607
Loc: Hollywoo

Last seen: 2 days, 17 hours

Re: AGAR ENVY! (Anything and All things agar!)

[Re: hogstomper]
#27413021 - 08/03/21 03:13 PM (5 years, 2 months ago)

I had a dream there were clouds in my coffee.

Transferred a bunch. only a few shown here.

Day 9 (date on one plate is wrong.)
75 degrees natural heat.

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--How to grow POPPIES.
--GROWING SHROOMS, FROM SPORES TO TRIPS
--x7x+ variety history.

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multifractal
Student

Registered: 05/09/15
Posts: 1,019
Loc: Cumtown

Re: AGAR ENVY! (Anything and All things agar!)

2
#27413061 - 08/03/21 03:56 PM (5 years, 2 months ago)

Some melmak clone plates I've been working with.

Edited by multifractal (08/03/21 04:04 PM)

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nektar61
Just a smear in the shale

Registered: 07/04/20
Posts: 4,607
Loc: Hollywoo

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Re: AGAR ENVY! (Anything and All things agar!)

[Re: DcevLearn]
#27413068 - 08/03/21 04:06 PM (5 years, 2 months ago)

DcevLearn said:

I'm running a nutrient variable experiment right now. Spent time over the weekend painstakingly making individual no-pours and precisely increased the amount of nutrients in each one. The baseline was 10cc of water in each no-pour and 0.2g of agar. Varied the LME from 0.5%, 1.0%, 1.5%, 2.0%, 2.5%, 3.0%, 3.5%, 4.0% and also BRF at 1.5% & 2.0%. Tonight I carefully moved over the 10 transfers. The dishes were slightly soupier than I would have preferred but I'm still really excited to see what they look like next week.

Will post a thread if it ends up panning out.

You know, right, that to produce any useful data from a "change one thing only" grow require using single culture clone selected down to a single sector and grown out.

Without that, there is too much variation possible with a multispore grow to really determine anything.

--------------------
--How to grow POPPIES.
--GROWING SHROOMS, FROM SPORES TO TRIPS
--x7x+ variety history.

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DcevLearn
Team Tomentose

Registered: 07/07/18
Posts: 385
Last seen: 1 year, 8 months

Re: AGAR ENVY! (Anything and All things agar!)

1
#27413323 - 08/03/21 07:42 PM (5 years, 2 months ago)

nektar61 said:

DcevLearn said:

I'm running a nutrient variable experiment right now. Spent time over the weekend painstakingly making individual no-pours and precisely increased the amount of nutrients in each one. The baseline was 10cc of water in each no-pour and 0.2g of agar. Varied the LME from 0.5%, 1.0%, 1.5%, 2.0%, 2.5%, 3.0%, 3.5%, 4.0% and also BRF at 1.5% & 2.0%. Tonight I carefully moved over the 10 transfers. The dishes were slightly soupier than I would have preferred but I'm still really excited to see what they look like next week.

Will post a thread if it ends up panning out.

You know, right, that to produce any useful data from a "change one thing only" grow require using single culture clone selected down to a single sector and grown out.

Without that, there is too much variation possible with a multispore grow to really determine anything.

Yeah I don't actually plan on publishing this one to the Journal of Mycological Sciences, maybe next time though. In my home lab there are obviously many other variables, sector selection, wedge size, accuracy of my measuring devices. Regardless it will still give me a sense for what works better with the products that I use.

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Last Project: LAGM: Stones From Scratch in 2020

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nektar61
Just a smear in the shale

Registered: 07/04/20
Posts: 4,607
Loc: Hollywoo

Last seen: 2 days, 17 hours

Re: AGAR ENVY! (Anything and All things agar!)

[Re: DcevLearn]
#27413755 - 08/04/21 06:31 AM (5 years, 2 months ago)

gettin' furry.

and

--------------------
--How to grow POPPIES.
--GROWING SHROOMS, FROM SPORES TO TRIPS
--x7x+ variety history.

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Lostkeys
Fractal Thought Pattern

Registered: 06/06/09
Posts: 1,355
Loc: Sirius
Last seen: 2 years, 10 months

Re: AGAR ENVY! (Anything and All things agar!)

1
#27413818 - 08/04/21 09:12 AM (5 years, 2 months ago)

I don't know who decided that nutritional yeast wasn't a necessary additive to MEA, but as soon as I included it, my growth got way healthier and faster. Up until then, all of my MEA plates were thin and wispy. Now I'm getting strong rhizomorphic growth.

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Stipe-n Cap
Full retard

Registered: 08/04/12
Posts: 9,117
Loc: Canada

Re: AGAR ENVY! (Anything and All things agar!)

[Re: Lostkeys]
#27413857 - 08/04/21 10:08 AM (5 years, 2 months ago)

I've only ever used LME and my plates are dope.

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monkey_accident
seraphim

Registered: 05/11/19
Posts: 710
Loc: im here, bro
Last seen: 4 months, 15 days

Re: AGAR ENVY! (Anything and All things agar!)

[Re: Stipe-n Cap]
#27413877 - 08/04/21 10:26 AM (5 years, 2 months ago)

:whathesaid:

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B H O

Registered: 03/17/13
Posts: 675
Last seen: 2 years, 7 months

Re: AGAR ENVY! (Anything and All things agar!)

[Re: multifractal]
#27413921 - 08/04/21 11:19 AM (5 years, 2 months ago)

Nice multi! My first cloned specimen are finally showing growth. It’s wavy capped Mazatapec. If it goes good, I’m going to store them and look for something leucistic to cross with.

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Guerrilla
Original Asshole

Registered: 01/30/21
Posts: 3,218
Loc: United Kingdom

Re: AGAR ENVY! (Anything and All things agar!)

[Re: B H O]
#27413988 - 08/04/21 12:02 PM (5 years, 2 months ago)

Does anybody have pictures of healthy Panaeolus Cyanescens cultures on agar?

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Being pissed on does not make you a real man.

...OR DOES IT?

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A.k.a
Stranger

Registered: 10/27/19
Posts: 18,224
Loc: Gaming the system
Last seen: 10 hours, 34 minutes

Re: AGAR ENVY! (Anything and All things agar!)

[Re: Guerrilla]
#27414036 - 08/04/21 12:28 PM (5 years, 2 months ago)

The pan thread. The link for jcms fc also had some.

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PhosCap
Gratuitous Heavenly Grace

Registered: 06/09/10
Posts: 975
Loc: Tartary
Last seen: 3 years, 5 months

Re: AGAR ENVY! (Anything and All things agar!)

[Re: A.k.a]
#27414481 - 08/04/21 06:27 PM (5 years, 2 months ago)

Working on a couple Pan projects.
Wild South Carolina cubensis
KSSS - this dish looks like a head dress
RW - ready for transferring to grain - Notice in the 11 oclock position some slight Rhizo activity. People say that RW doesn't produce rhizo's.
PE6 - ready to send to grain

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Festivusmiracle
Stranger

Registered: 06/04/21
Posts: 126
Loc: Flyover state, USA
Last seen: 2 years, 11 months

Re: AGAR ENVY! (Anything and All things agar!)

[Re: A.k.a]
#27414483 - 08/04/21 06:28 PM (5 years, 2 months ago)

I apologize if this is the wrong thread but is definitely agar related. What is this pattern of growth due to? All of my T4 dishes look like this from MSS to agar. Symmetrical growth and no obvious contamination that I can see but the immediate 1 cm or so from the transfer wedge looks different than the rest of the growth. Is it something that I should transfer an additional round before going to grains? Thanks for any advice!

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A.k.a
Stranger

Registered: 10/27/19
Posts: 18,224
Loc: Gaming the system
Last seen: 10 hours, 34 minutes

Re: AGAR ENVY! (Anything and All things agar!)

[Re: Festivusmiracle]
#27414491 - 08/04/21 06:37 PM (5 years, 2 months ago)

Idk why that happens sometimes. That plate looks clean to me.

Been a while since I’ve had one myself.

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ReverendMyc
succinct is not my forte

Registered: 03/29/19
Posts: 3,528
Loc: uacious, but can't spel gud

Re: AGAR ENVY! (Anything and All things agar!)

[Re: A.k.a]
#27414495 - 08/04/21 06:47 PM (5 years, 2 months ago)

Just a theory, but I suspect that it happens to me when my agar has some condensation that has not reabsorbed yet. The myc grows like that when it is wetter and then forms more nicely once the moisture is more ideal.

Could be pure bs, though. I have a few fresh plates right now that I can detect a bit of surface moisture rolling around on if I tilt them too much. Otherwise it is pretty invisible. But in a few days, they will be dry if they follow the same pattern. I will try to grab pics when I get home.

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Stoned Gummys | BRF Pucks | Primo Reusable Plates | Easy LI 4 Preserv & Propo"Psychedelics are powerful substances. Nothing that powerful is completely safe... and nothing completely safe is that powerful!" - Abigail Calder at ALPS 2023
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Festivusmiracle
Stranger

Registered: 06/04/21
Posts: 126
Loc: Flyover state, USA
Last seen: 2 years, 11 months

Re: AGAR ENVY! (Anything and All things agar!)

[Re: ReverendMyc]
#27414501 - 08/04/21 07:03 PM (5 years, 2 months ago)

Thanks y’all! I’ll get some grains ready :crazy2:

:crazy2:

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Paracosm

Registered: 07/04/21
Posts: 808
Loc: Floating in Space
Last seen: 3 years, 25 days

Re: AGAR ENVY! (Anything and All things agar!)

[Re: Festivusmiracle]
#27414597 - 08/04/21 07:59 PM (5 years, 2 months ago)

On the 30th I did my second run of agar using the clock streaking method. So far, there's nothing visible on any of the other plates except these two.

Amazonian Print

I'm totally baffled by how this even happened. I have no idea if it's any good or why it even grew this way. The little sliver line on the edge above the writing is 12 o'clock. Also, this grew rapidly all things considered.

A.P.E. Swab

Then there's this. There's obvious contamination on the side there. I think I see something near the swab itself, but at this point I'm conceding that I really have no clue what I'm looking for in the first place. I'm not sure if I should let this sit awhile longer, or if I should try and grab a sample from near the swab where a little tendril has grown out.

I still have plenty of clean untouched plates from pouring. Of the stack, it appears one or two are contaminated just sitting in my SAB with the arm holes covered. I feel frustrated since I haven't even touched anything in there since pouring. I'm not sure what I'm doing wrong. :uhoh:

:uhoh:

Any help would be appreciated. Thanks for looking and reading.

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My Grow Journal

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Shop:

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