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Shop: North Spore Injection Grain Bag   Myyco.com Isolated Cubensis Liquid Culture For Sale

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Re: AGAR ENVY! (Anything and All things agar!) [Re: SpaceBaby]
    #27391943 -

SpaceBaby said:
Go for the 500 ml., I make uop 400 ml agar with which you can pour ~20 petris. It seems innocent. I now have 8 whcih just fit the 23-qy presto. you are going to be blasting through agar and petris. I just took delivery on 1000 celltreat petris. i am in love.


I saw those on Amazon. These are the ones I picked up for a quick fix. I'm going to invest in something like these once I get the groove of things.

I really like the idea of the 250ml's just for the simple fact that I'll probably do a bit of color coding, but I hear what you're saying regarding quantity.


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My Grow Journal

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Lovage]
    #27391950 -

Lovage said:
Agreed. Celltreat dishes are nice. And they will send you a free sample sleeve :smile:. Mine are almost gone at this point. Gonna have to purchase some now it seems lol.


a thousand, dood. and really, how long will they last.


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SpaceBaby
SPACEBABY'S LAGM22 THREAD
MUSHBOY'S SHROOM TEA TEK

Me as a cube

Another Day's Work:


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Re: AGAR ENVY! (Anything and All things agar!) [Re: Lovage]
    #27391956 -

Lovage said:
Agreed. Celltreat dishes are nice. And they will send you a free sample sleeve :smile:. Mine are almost gone at this point. Gonna have to purchase some now it seems lol.



Apologies for the newbie question, but are these reusable or are they a one and done deal? I'm planning on going to glass for some of the long term storage stuff, but I haven't had any experience with disposables/plastics yet.


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My Grow Journal

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Paracosm]
    #27391959 -

So this is what I expect to be trich mycelium piggyback or another mold. The very leading edge you can see healthy rhizo mycelium, but everywhere else is a thin layer of fuzzy growth covering the mycelium.

Does anyone have an idea of how you end up at this point? I've sometimes been in the bad habit of not taking transfers from the very leading edges, which I suspect doesn't help the cause. But at some point I am literally grabbing this mold from other plates most of which originate as clones, and can't seem to get away from it in some cases. I'm trying to nail down where it originates if it's probably in my choice area of selection. If a plate is technically clean will grabbing from or near the center still pose a risk for contam on further plates?

I've been using a new corer tool to make circle cuts, and potentially I'm not sterilizing it red hot in between transfers, my scalpel blade gets red in 7 seconds but the tool is thicker, and I also only sterilize for 7 seconds, maybe should bump that up to 20-30.



--------------------
"In The Material World One seeks retirement and grows Old
In The Magical World One seeks Enlightenment and grows Wiser
In The Miraculous World One seeks nothing and grows Lighter
As we all tread the Homeward Path we will explore many Realms
And one day... we will all Realize that all experiences are Simply
Different ways in which The
All-That Is
Perceives Itself"

Edited by Eclipse3130 (07/18/21 12:46 AM)

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Eclipse3130]
    #27391988 -

edited.

Edited by aNewBeginning (11/08/21 02:17 PM)

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Paracosm]
    #27391992 -

Paracosm said:
Lovage said:
Agreed. Celltreat dishes are nice. And they will send you a free sample sleeve :smile:. Mine are almost gone at this point. Gonna have to purchase some now it seems lol.



Apologies for the newbie question, but are these reusable or are they a one and done deal? I'm planning on going to glass for some of the long term storage stuff, but I haven't had any experience with disposables/plastics yet.


They're disposable.  They're made of polyethylene plastic which melts lower than heat sterilization temps, they're sterilized after manufacture via gamma radiation. 
Polypropylene (often referred to as 'PP5' around here - PP being its common abbreviation, 5 being its recycling code) plastic can handle heat sterilization temps.

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Eclipse3130]
    #27391993 -

Eclipse3130 said:



Looks good to me but I know nothing. Wouldn’t trich be be sporing by this amount of development?


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Dear God, did you see all the boobs on that guy…

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Eclipse3130]
    #27391999 -

Eclipse3130 said:
I've been using a new corer tool to make circle cuts, and potentially I'm not sterilizing it red hot in between transfers, my scalpel blade gets red in 7 seconds but the tool is thicker, and I also only sterilize for 7 seconds, maybe should bump that up to 20-30.


I once moved over to metal straws for circular transfers.
You get them glowing red faster than your scalpel.
Also the angle makes them very comfortable to work with, maybe give´em a try :wink:

                    :cookiemonster:


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Re: AGAR ENVY! (Anything and All things agar!) [Re: Goatrider]
    #27392003 -

there is no need for glowing red



most anything should be killed by 300f.


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- Sandbag Tek - How To Sterilize Spawn Bags - All About Static Pressure / Pressure Drop for DIY Flow Hoods - Sandman's LC Tek-

Marijuanaut escapes earth to cultivate - Grow-room is church temple of the new stoner breed

Edited by sandman420 (07/18/21 01:23 AM)

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Goatrider]
    #27392005 -

I need a new transfer tool. Where ya get these straws? They got a specific name?


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Dear God, did you see all the boobs on that guy…

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Lovage]
    #27392017 -

Lovage said:
I need a new transfer tool. Where ya get these straws? They got a specific name?


Just metal straws, something like that:
Click me!

                    :cookiemonster:


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Re: AGAR ENVY! (Anything and All things agar!) [Re: Goatrider]
    #27392022 -

Ss cocktail straws are a little shorter and handle nicely.


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D3’s Glog
D3's Agar TEK
Agar Porn Gallery
RW x MelMak Grow Along Thread

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Paracosm]
    #27392109 -

Paracosm said:
Lovage said:
Agreed. Celltreat dishes are nice. And they will send you a free sample sleeve :smile:. Mine are almost gone at this point. Gonna have to purchase some now it seems lol.



Apologies for the newbie question, but are these reusable or are they a one and done deal? I'm planning on going to glass for some of the long term storage stuff, but I haven't had any experience with disposables/plastics yet.


use once. stay away from glass petris, I have been advised...pita.

long-term storage of cultures: slants in glass test tubes with screw tops. there's at least one tek and RR vids.


--------------------
SpaceBaby
SPACEBABY'S LAGM22 THREAD
MUSHBOY'S SHROOM TEA TEK

Me as a cube

Another Day's Work:


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Re: AGAR ENVY! (Anything and All things agar!) [Re: SpaceBaby]
    #27392653 -

I'll stick to the PP5's until I do a bit more research then. Thanks for the information, everyone.


--------------------
My Grow Journal

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Paracosm]
    #27392681 -

Target has the metal straws. A six pack was like 7 bucks. I think.

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Paracosm]
    #27392688 -

Paracosm said:
I'll stick to the PP5's until I do a bit more research then. Thanks for the information, everyone.


You guys are gonna force me to do a glass petri Tek.


--------------------
It takes guts to be
gentle and kind

Another way to live

Your ego is your nemesis.
Seek the truth tirelessly,
Even at the cost of your own sanity.
-fiddle_head

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Re: AGAR ENVY! (Anything and All things agar!) [Re: LadysKnight]
    #27392709 -



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D3’s Glog
D3's Agar TEK
Agar Porn Gallery
RW x MelMak Grow Along Thread

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Re: AGAR ENVY! (Anything and All things agar!) [Re: D3_Myc]
    #27392716 -

D3monic said:



SEXY HAS LANDED!!


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Dear God, did you see all the boobs on that guy…

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Re: AGAR ENVY! (Anything and All things agar!) [Re: LadysKnight]
    #27392885 -

LadysKnight said:
Paracosm said:
I'll stick to the PP5's until I do a bit more research then. Thanks for the information, everyone.


You guys are gonna force me to do a glass petri Tek.


waiting


--------------------
SpaceBaby
SPACEBABY'S LAGM22 THREAD
MUSHBOY'S SHROOM TEA TEK

Me as a cube

Another Day's Work:


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Re: AGAR ENVY! (Anything and All things agar!) [Re: Paracosm]
    #27393007 -

Paracosm said:
I'll stick to the PP5's until I do a bit more research then. Thanks for the information, everyone.


freedom is a beautiful thing

me, i love these

https://www.celltreat.com/product/229693/


--------------------
SpaceBaby
SPACEBABY'S LAGM22 THREAD
MUSHBOY'S SHROOM TEA TEK

Me as a cube

Another Day's Work:


Extras: Filter Print Post Top
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Shop: North Spore Injection Grain Bag   Myyco.com Isolated Cubensis Liquid Culture For Sale

Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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