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verum subsequentis
seeker of truth

Registered: 03/22/16
Posts: 8,732
Last seen: 4 years, 4 months

Re: AGAR ENVY! (Anything and All things agar!)

[Re: Stipe-n Cap]
#27386668 - 07/13/21 10:50 PM (5 years, 2 months ago)

p9hu7 said:

I would probably just stop culting if I couldn't use a hood, it's just too convenient and awesome.

@spacebaby

I got my zapper from ebay.

How do you cut your cling wrap?

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PhosCap
Gratuitous Heavenly Grace

Registered: 06/09/10
Posts: 975
Loc: Tartary
Last seen: 3 years, 4 months

Re: AGAR ENVY! (Anything and All things agar!)

[Re: PhosCap]
#27386672 - 07/13/21 10:57 PM (5 years, 2 months ago)

Working with a wild cube print from South Carolina. I dumped so many spores not on purpose, they were free and loose on the foil. An aggressive start so far for the myc.

This dish appears to have some possible trich in the upper region. need to do a transfer very soon. Thinking of the section on the farthest left

This dish appears to be clean. How does it look. The condensation doesn't help with observation.

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Biskop
Fokken Prawns

Registered: 12/09/17
Posts: 192
Loc: Vietnam 🇻🇳
Last seen: 4 years, 4 months

Re: AGAR ENVY! (Anything and All things agar!)

[Re: Tormato]
#27386751 - 07/14/21 02:21 AM (5 years, 2 months ago)

Hi guys, I have a failed lunchbox with some pretty contaminated grains. I don’t know how long it’s been sitting there but I’ve dug through and found some “healthy” mycelium(not healthy it’s contammed af but it still smells good). Any tips for putting a contaminated grain to agar or do I just hope for the best ?

Here’s a pic of my mould box:

Pretty fucked…

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SpaceBaby
alchemist

Registered: 11/01/20
Posts: 2,030
Loc: SPACE
Last seen: 3 years, 24 days

Re: AGAR ENVY! (Anything and All things agar!)

[Re: Biskop]
#27386790 - 07/14/21 04:10 AM (5 years, 2 months ago)

Fuck.
I have AGAR ENVY indeed.
I started to make up 3200 ml of agar last night and discovered I'm out of agar. Doh! I dont want to wait foe the delivery; I have a gazillion transfers overdue for the taking.

Hopefully, the Oriental Food Mart a mile and a half away will have Telephone Brand in stock.

For FUCK'S sake.

:facepalm3:

:facepalm3:

Anybody have a url for that hole punch tool you aesthetes use to take transfers?

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Mr. Mushie

Registered: 01/16/20
Posts: 667

Re: AGAR ENVY! (Anything and All things agar!)

[Re: verum subsequentis]
#27386812 - 07/14/21 05:08 AM (5 years, 2 months ago)

p9hu7 said:

I remember you posting about contams on plates while using your hood.

This is how I've made every transfer, no satellites or mold. Keep in mind that every surface, especially the rack, has been thoroughly sanitized with ISO.

Bactizappers are dope af. I need to set my big hood up again : (

The field of view is restricted so that my tattoos aren't visible. It's t-shirt season.

I'm thinking about using plasti dip on the top of my rack so plates don't move around when making transfers.

Also:

verum subsequentis said:

How do you cut your cling wrap?

:whathesaid:

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Lenz
Misunderestimated

Registered: 08/17/20
Posts: 692
Loc: Fungistan

Re: AGAR ENVY! (Anything and All things agar!)

[Re: Mr. Mushie]
#27386855 - 07/14/21 06:24 AM (5 years, 2 months ago)

PVC cutter for cling wrap, or so I've heard.

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:chems:

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A.k.a
Stranger

Registered: 10/27/19
Posts: 18,222
Loc: Gaming the system
Last seen: 29 minutes, 45 seconds

Re: AGAR ENVY! (Anything and All things agar!)

[Re: Lenz]
#27386868 - 07/14/21 06:42 AM (5 years, 2 months ago)

Yeah or hacksaw.

Box cutter works too but you gotta keep it lined up perfect as you turn.

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D3_Myc
Weeb Trash

Registered: 05/06/18
Posts: 4,399
Loc: Year Zero

Re: AGAR ENVY! (Anything and All things agar!)

[Re: A.k.a]
#27386870 - 07/14/21 06:45 AM (5 years, 2 months ago)

Tried the chop saw once… just melted the edges together 🤣

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Stipe-n Cap
Full retard

Registered: 08/04/12
Posts: 9,112
Loc: Canada

Re: AGAR ENVY! (Anything and All things agar!)

[Re: verum subsequentis]
#27386902 - 07/14/21 07:18 AM (5 years, 2 months ago)

verum subsequentis said:

How do you cut your cling wrap?

I've always used a very sharp butchers knife, and some elbow grease. If it's good and sharp it always comes out with nice smooth edges.

Not sure why the fuck this is in bold letters, lol.

I hate it when my plates slide, that may be a good idea.

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mushboy
modboy

Registered: 04/24/05
Posts: 40,590
Loc: the woods

Re: AGAR ENVY! (Anything and All things agar!)

[Re: Stipe-n Cap]
#27386903 - 07/14/21 07:20 AM (5 years, 2 months ago)

:douchewink:

forgot the [ / b ]

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mushboy
modboy

Registered: 04/24/05
Posts: 40,590
Loc: the woods

Re: AGAR ENVY! (Anything and All things agar!)

[Re: mushboy]

2
#27386940 - 07/14/21 08:02 AM (5 years, 2 months ago)

can always count on some agar pins!

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:hypnotoad:

:hypnotoad:

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B H O

Registered: 03/17/13
Posts: 675
Last seen: 2 years, 6 months

Re: AGAR ENVY! (Anything and All things agar!)

[Re: mushboy]
#27387041 - 07/14/21 09:19 AM (5 years, 2 months ago)

mushboy said:

can always count on some agar pins!

How do you get pinning to form on agar? Is it just time?

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Lovage
Full Time Ding Dong

Registered: 04/22/19
Posts: 380

Re: AGAR ENVY! (Anything and All things agar!)

[Re: B H O]

1
#27387063 - 07/14/21 09:31 AM (5 years, 2 months ago)

if you can get yourself a wooden dowel that fits inside the paper tube then it makes it much easier to apply a solid pressure and roll the roll in a straight line. cut with a sharp kitchen knife. works really really well

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D3_Myc
Weeb Trash

Registered: 05/06/18
Posts: 4,399
Loc: Year Zero

Re: AGAR ENVY! (Anything and All things agar!)

[Re: B H O]

1
#27387075 - 07/14/21 09:37 AM (5 years, 2 months ago)

B H O said:

mushboy said:

can always count on some agar pins!

How do you get pinning to form on agar? Is it just time?

Yea just time, usually a month or sometimes 2

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mushboy
modboy

Registered: 04/24/05
Posts: 40,590
Loc: the woods

Re: AGAR ENVY! (Anything and All things agar!)

[Re: D3_Myc]

1
#27387108 - 07/14/21 10:07 AM (5 years, 2 months ago)

Usually 4weeks for me. That plate was inoculated 6/11.

--------------------
:hypnotoad:

:hypnotoad:

Making Liquid Culture : Go insane with LC
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tututotutut
You...! You're not me!

Registered: 01/18/21
Posts: 2,218
Last seen: 5 years, 2 months

Re: AGAR ENVY! (Anything and All things agar!)

[Re: mushboy]
#27387552 - 07/14/21 03:38 PM (5 years, 2 months ago)

Often corner of otherwise I'll have some dishes that for whatever (guessing bacterial, i dont spawn or transfer those). I'll burying them outside save them up and last couple times i just threw them100% in a creek head.I've tried,already been cut up a ton, some that get kinda What do you do
I get a lot of agar with them? Like this that I feel isn't usable abundance.. Idk maybe build a pile, something could spread out there
Im not talking blotchy healthy dishes
Dishes with like a single contam spot in a good growth, one thats reason, I do not want to use for transfers anymore

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yeah keep telling yourself that's voluntary

Edited by tututotutut (07/15/21 10:50 AM)

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tututotutut
You...! You're not me!

Registered: 01/18/21
Posts: 2,218
Last seen: 5 years, 2 months

Re: AGAR ENVY! (Anything and All things agar!)

[Re: D3_Myc]
#27387558 - 07/14/21 03:39 PM (5 years, 2 months ago)

D3monic said:

B H O said:

mushboy said:

can always count on some agar pins!

How do you get pinning to form on agar? Is it just time?

Yea just time, usually a month or sometimes 2

Ahhh i knew some TCs did this
In another agar thread someone had told me its taboo to attempt growing dishes out fully, i suppose they haven't seen these posts

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yeah keep telling yourself that's voluntary

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mushboy
modboy

Registered: 04/24/05
Posts: 40,590
Loc: the woods

Re: AGAR ENVY! (Anything and All things agar!)

[Re: tututotutut]

2
#27387573 - 07/14/21 03:45 PM (5 years, 2 months ago)

Attempt?

:lolwut:

... what?

:what:

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:hypnotoad:

Making Liquid Culture : Go insane with LC
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LtLurker
Lost Sailor

Registered: 01/03/18
Posts: 7,537
Loc: Borderlands

Last seen: 1 year, 8 months

Re: AGAR ENVY! (Anything and All things agar!)

[Re: mushboy]
#27387589 - 07/14/21 03:53 PM (5 years, 2 months ago)

:callingbullshit:

You're absolutely leaving out context. The only time you'd want to avoid letting it grow to edges is when you're selecting for transfers. You can see the sectors easier. We could stretch it to say you're also not gonna get some satellite contam from the edge of the dish.

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tututotutut
You...! You're not me!

Registered: 01/18/21
Posts: 2,218
Last seen: 5 years, 2 months

Re: AGAR ENVY! (Anything and All things agar!)

[Re: LtLurker]
#27387681 - 07/14/21 05:07 PM (5 years, 2 months ago)

mushboy said:

Attempt?

... what?

Attempt as in attempt creating and using a dish that colonizes all the way without any contamination or hidden bacteria.

LtLurker said:

You're absolutely leaving out context. The only time you'd want to avoid letting it grow to edges is when you're selecting for transfers. You can see the sectors easier. We could stretch it to say you're also not gonna get some satellite contam from the edge of the dish.

Sure. Regardless I had someone fuss at me about having some dishes older than two weeks implying they should never get that old. Who knows.

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yeah keep telling yourself that's voluntary

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Shop:

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