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milkboy
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Registered: 05/01/21
Posts: 2,743

Re: AGAR ENVY! (Anything and All things agar!)

[Re: Atomsplit]
#27360415 - 06/23/21 07:10 PM (5 years, 4 months ago)

The purpose of transferring is to ensure clean mycelium. If you transfer early there is less time for something to intertwine with clean mycelium, this is also why people tend to streak their plates from my understanding. I didn't do any of this my first go and ended up with mold on my mycelium :frown:

:frown:

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Eugene Gesuale
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Re: AGAR ENVY! (Anything and All things agar!)

[Re: milkboy]
#27360432 - 06/23/21 07:29 PM (5 years, 4 months ago)

I transfer quick so I can get clean quick for grain quick. You can shave a week or two or more by taking sooner rather than later

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Re: AGAR ENVY! (Anything and All things agar!)

[Re: Eugene Gesuale]
#27360609 - 06/23/21 09:39 PM (5 years, 4 months ago)

With germ plates I usually don’t transfer until things have grown out a little bit so I can see the different growths.

I do try to take as small a wedge as possible with germ plates and t1s to narrow the genetics and to try and get a clean sample. The thing with spore plates is you never know where hidden contam is so the smaller the better.

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Mr. Mushie

Registered: 01/16/20
Posts: 667

Re: AGAR ENVY! (Anything and All things agar!)

[Re: Crackatoa]
#27361107 - 06/24/21 07:35 AM (5 years, 4 months ago)

Crackatoa said:

Here's my straw and scalpel, I do wanna get one of those fancy ones though. Here's Jack Frost.

Is the point of keeping the scalpel in the flow with a magnet just to ensure it gathers less poop on it if you were to set it down between transfers?

I've seen this implemented a lot and never thought much of it because I flame my scalpel well between every transfer.

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Crackatoa
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Re: AGAR ENVY! (Anything and All things agar!)

[Re: Mr. Mushie]
#27361232 - 06/24/21 10:20 AM (5 years, 4 months ago)

I flame between plates but it keeps it on the flow after it's torched and keeps it out of my work space in front of my hood. I'll flame it well, stick it there to cool while I wipe my plates down and it's there ready when I am.

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Atomsplit

Registered: 01/17/21
Posts: 1,578
Loc: SAB

Re: AGAR ENVY! (Anything and All things agar!)

[Re: Crackatoa]
#27361634 - 06/24/21 04:46 PM (5 years, 4 months ago)

Thanks for the help.

I may be working with dirty prints. After 3 weeks and many attempts. I've come up with mold or bacterial transfers/ plates.

Here's more plates that are off different spots on the print.

They look bad to me and I just think I should move on to getting more prints.

Do you agree?

Thanks again!

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multifractal
Student

Registered: 05/09/15
Posts: 1,019
Loc: Cumtown

Re: AGAR ENVY! (Anything and All things agar!)

[Re: Atomsplit]
#27361638 - 06/24/21 04:48 PM (5 years, 4 months ago)

They look OK possibly. I would make some transfers and see how the T1 plates look.

Edited by multifractal (06/24/21 04:49 PM)

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Big_Dub
I'm just some guy

Registered: 01/12/11
Posts: 3,649
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Re: AGAR ENVY! (Anything and All things agar!)

[Re: multifractal]
#27361645 - 06/24/21 04:50 PM (5 years, 4 months ago)

:whathesaid:

Working with dirty prints can be good practice haha

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Atomsplit

Registered: 01/17/21
Posts: 1,578
Loc: SAB

Re: AGAR ENVY! (Anything and All things agar!) *DELETED*

[Re: Big_Dub]
#27361661 - 06/24/21 05:02 PM (5 years, 4 months ago)

Sorry moved question to starting over

Edited by Atomsplit (06/24/21 07:08 PM)

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Lectric
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Registered: 08/31/20
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Re: AGAR ENVY! (Anything and All things agar!)

[Re: Atomsplit]
#27361667 - 06/24/21 05:05 PM (5 years, 4 months ago)

1st APE transfer from MS plate.
Thoughts?

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Big_Dub
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Re: AGAR ENVY! (Anything and All things agar!)

[Re: Lectric]
#27361673 - 06/24/21 05:08 PM (5 years, 4 months ago)

Time for t2

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multifractal
Student

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Re: AGAR ENVY! (Anything and All things agar!)

[Re: Big_Dub]

1
#27361854 - 06/24/21 07:52 PM (5 years, 4 months ago)

Am I right to assume the white clumps on the TAT germ plate are hyphal knots? Also, some nasty contamination, bacteria or mold?, being overtaken by mycelium. Go myc, go!

Beautiful Mel plate. I'll be using this plate to make a round of inoculation plates and some LI, blender jar all ready to go.

Edited by multifractal (06/24/21 07:53 PM)

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D3_Myc
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Registered: 05/06/18
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Re: AGAR ENVY! (Anything and All things agar!)

[Re: multifractal]

1
#27361872 - 06/24/21 08:12 PM (5 years, 4 months ago)

--------------------

D3’s GlogD3's Agar TEKAgar Porn Gallery RW x MelMak Grow Along Thread

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Big_Dub
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Registered: 01/12/11
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Re: AGAR ENVY! (Anything and All things agar!)

[Re: D3_Myc]
#27362067 - 06/24/21 10:45 PM (5 years, 4 months ago)

June 05
Ovoid print to brf agar

June 11

June 15

June 22

Transfer to grainsoak agar

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Edited by Big_Dub (06/25/21 01:13 PM)

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moshe1

Registered: 08/29/15
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Re: AGAR ENVY! (Anything and All things agar!)

[Re: Big_Dub]

1
#27362132 - 06/25/21 12:12 AM (5 years, 4 months ago)

a T1 melmak plate i took transfers out of, but gonna put to grains to test since its clean already.

a snapshot of some of those melmaks, mixed t2/t3s. As you could see either very fuzzy looking or weak- mostly clean though i think.

one BRF plate grew out pretty out of the batch of BRF agar i tested so far

a couple of the BBMS that took the lead from my plates- finally can test some wedges next week including these

Logging all this here

Edited by moshe1 (06/25/21 12:14 AM)

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Big_Dub
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Re: AGAR ENVY! (Anything and All things agar!)

[Re: moshe1]
#27362893 - 06/25/21 04:44 PM (5 years, 4 months ago)

Good shit m8

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SpaceBaby
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Registered: 11/01/20
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Re: AGAR ENVY! (Anything and All things agar!)

[Re: D3_Myc]
#27363095 - 06/25/21 07:01 PM (5 years, 4 months ago)

D3monic said:

:ohmyfuck:

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moshe1

Registered: 08/29/15
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Re: AGAR ENVY! (Anything and All things agar!)

[Re: SpaceBaby]
#27363190 - 06/25/21 08:10 PM (5 years, 4 months ago)

trying to fruit and test this very juicy looking invitro pin plate. The PEU clone was from a small cluster that just pinned, which i took straight to agar- and then that dish through this pin.. the transfers are looking kinda, not as juicy though. the plate above is now in the fridge while i see what happens with these. Wondering if i should save that plate while i test these transfers or fruit it as well. What do you think, do the xfers look okay? lookin a bit hairy there.

same plates, side views

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Edited by moshe1 (06/25/21 10:22 PM)

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LadysKnight
Hello Ladies

Registered: 10/09/15
Posts: 3,349
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Re: AGAR ENVY! (Anything and All things agar!)

[Re: moshe1]
#27363210 - 06/25/21 08:23 PM (5 years, 4 months ago)

Your cultures have been looking great for a while now.
Anything you decide to do with those will succeed.

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Re: AGAR ENVY! (Anything and All things agar!)

[Re: LadysKnight]
#27363217 - 06/25/21 08:29 PM (5 years, 4 months ago)

:whathesaid:

Those are clean as fuck. Clean myc from a solid culture is as good as it gets.

It does look weird when the myc puffs up on the wedge, I’ve had some go up to the lid before.

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