Normlizer420 said:

I made this using a spent rifle case and old scalpel.

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Inthepit said:

Speaking of pin head size transfers.
What about just lifting a piece of myc off the agar.

I did because I fumbled one wedge and then poked the surface
lifting a thread like piece of just myc.

Not sure where that one went, lost track. I recall
it seemed to take awhile to grow.

But it didn't seem to need a wedge under it or over it.

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Inthepit said:

Ya, a sliver of myc. I'm just waiting for the more experienced like Mushboy,
I keep ribbing him because I like him.  Or others...

But there is a thread describing how faceplanting a wedge,
the myc crawls out from under and contam has a harder time getting out.

So there's that too...

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trippytimes said:

Yeah idk why x7x like to keep it s'mores. I have the ape getting crazy looking after my third transfer it looks all rizo! I'm excited! Already have LC for golden halo and ape going.

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that's cool. i had one t1 go super rhizo on me. i wish i had used the 2mm punch on those.

D3monic said:

A couple plates up for xfers tonight. Including a few plates from the agar dye experiment. Will be taking the puck off the red plate and seeing if it recovers on a different color plate or if it totally hindered it.

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Quote:

p9hu7S said:

Quote:

tryptkaloids said:
Its not something I'd suggest starting with but if you know what you are doing its pretty nifty

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This is pretty much my point. Most people doing this, with a few exceptions, are the sort of people that have trouble producing clean cultures/spawn to begin with. Adding extra steps will not help.

Though there may be fewer hand motions in terms of actual cutting motions, the exposure is greater. 2 tools instead of one, hand exposure by hovering or to manipulate the plate so as not to hover, and increased surface area of transfer.

3 simple cuts is all I make, they are extremely small. Transfer is approximately the size of a pin head.just enough to skewer with my scalpel. 4 tiny/quick motions in total.

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I am pretty inexperienced. Only done 100 plates so far and I work in a SAB.

I tried the steel straw technique a few times because I think it looks beautiful. But I found it to be very inconvenient.

If you don't bend the straw, your whole hand goes over the plate. Bending the straw is better, but still kinda awkward motion to stamp the plate with. And the plates I did this with got contaminated.



Also found it to be more work to switch between tools:
First flame the straw, then cool the straw in the receiving plate. Then stamp the area on the plate I want to transfer. Then flame the scalpel. Cool down scalpel in the receiving plate. And finally moving the stamped agar piece with the scalpel.

By only using scalpel there is much less movement and faster transfer. But of course doesn't look so nice. If I had a flow hood I guess it would be easier to work with a tool like that.