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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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Re: AGAR ENVY! (Anything and All things agar!) [Re: MadHatter333]
    #26872857 -

Changing nutes can be good for identifying contaminants if you’re not sure how clean your culture is. People say that lowering nutes in general will tend to give more rhizo growth but it’s not a hard and fast rule.

Myc jumps between tomentose and rhizo for me, sometimes on the same plate. Just genetics I guess:shrug:


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Re: AGAR ENVY! (Anything and All things agar!) [Re: Yeetusdeetus]
    #26872880 -

The same culture can be manipulated to go tomentose or rhizo.

I use lme and using really low nutes, like 4g per 500ml and then pouring the plates on the thin side will usually produce rhizo ime.


This is a tamp plate, I think it’s a mutated cap that grew.



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LAGM2020

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Re: AGAR ENVY! (Anything and All things agar!) [Re: MadHatter333]
    #26872894 -

MadHatter333 said:
I’ve noticed ever since I started using LME I don’t get nice rizo growth like I used to with the same amount of BRF. I may switch back to BRF because I’m on my second transfer and am mostly getting tom growth. Anyone here like BRF over other nutes?


What’s your ratios? 20g agar to 14g lme works great for me


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D3’s Glog
D3's Agar TEK
Agar Porn Gallery
RW x MelMak Grow Along Thread

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Re: AGAR ENVY! (Anything and All things agar!) [Re: D3_Myc]
    #26872907 -

D3monic, what brand agar do you use? I use telephone and it’s pretty fuckin solid at 14g/l. Or do you just prefer stiffer agar?

I just did my first warm pours at that ratio but I don’t know if it was too hard for the myc


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Re: AGAR ENVY! (Anything and All things agar!) [Re: Yeetusdeetus]
    #26872968 -

I have an old agar dish that started to pin. My question is what's the best way to transfer that pin to a new dish?


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Re: AGAR ENVY! (Anything and All things agar!) [Re: Zeppelinthecat]
    #26872977 -

In front of a fh or in a sab, the same way you do any other transfer.


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"it's all a joke between mom contractions and coffin fittings"


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Re: AGAR ENVY! (Anything and All things agar!) [Re: D3_Myc]
    #26873055 -

D3monic said:
MadHatter333 said:
I’ve noticed ever since I started using LME I don’t get nice rizo growth like I used to with the same amount of BRF. I may switch back to BRF because I’m on my second transfer and am mostly getting tom growth. Anyone here like BRF over other nutes?


What’s your ratios? 20g agar to 14g lme works great for me


I’m using 8g LME to 8g agar in just over 400ml of water. I used to use the same ratio with BRF and I’d get some great plates. They have some rhizo now on the second transfer but at first it was more tomentose.


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Re: AGAR ENVY! (Anything and All things agar!) [Re: MadHatter333] * 1
    #26873165 -

To transfer plate pins I just stabbed them with the tip of the scalpel and they pop right off.


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LAGM2020

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Re: AGAR ENVY! (Anything and All things agar!) [Re: A.k.a]
    #26873620 -

Is it really much of a difference in colonization Rhizo vs Tom mycelium? Also does rhizo perform better in fruiting too? I’ve always went after rhizo but is Tom just as good? I want to use these second transfer plates in my master jars but the myc isn’t as strong as I was getting on BRF. Let me know your opinions please :smile: there’s some rhizo growth in them too, it’s kind of a mix between the two.


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Re: AGAR ENVY! (Anything and All things agar!) [Re: MadHatter333]
    #26873636 -

That seems to be a perpetual debate :smile: I like rhizo growth too but tomentose looking cultures can perform just as well ime


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Re: AGAR ENVY! (Anything and All things agar!) [Re: Yeetusdeetus]
    #26873649 -

Yeetusdeetus said:
D3monic, what brand agar do you use? I use telephone and it’s pretty fuckin solid at 14g/l. Or do you just prefer stiffer agar?

I just did my first warm pours at that ratio but I don’t know if it was too hard for the myc


Sorry, I didn’t specify. Those ratios are to 1000ml distilled water


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D3’s Glog
D3's Agar TEK
Agar Porn Gallery
RW x MelMak Grow Along Thread

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Re: AGAR ENVY! (Anything and All things agar!) [Re: D3_Myc]
    #26873668 -

I figured lol, if it were any less you’d basically be cutting glass :laugh2:


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Re: AGAR ENVY! (Anything and All things agar!) [Re: Yeetusdeetus]
    #26873812 -

from my understanding of rhizomorphic vs tomentose the rhizomorphic is going to be more sought after. The rhizomorphic growth is a different mycelial structure that is designed for long stretches searching for food, whereas tomentose is just trying to help it breathe.

I could be wrong though, this is shit I've read and i don't have any of my own work to submit as proof


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Mehhh



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Re: AGAR ENVY! (Anything and All things agar!) [Re: Wall.E]
    #26873888 -

Day 10
Sectors are looking great!
Should probably start to isolate, but from where? :confused:



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Treasure Coast - agar, oat, coir, monotub

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Re: AGAR ENVY! (Anything and All things agar!) [Re: MTManiac]
    #26873924 -

I really don’t notice much difference between types of growth besides rhizo being slightly faster, but most of the time it all looks the same once it hits grain.

My very fastest cultures have been rhizo though all the way from the plate to running the coir.


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LAGM2020

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Re: AGAR ENVY! (Anything and All things agar!) [Re: A.k.a]
    #26874093 -

Thanks for the info and different opinions on the two growths. I’ll try for rhizo but try the other too to see what I come up with. Will map it out and research more.


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Re: AGAR ENVY! (Anything and All things agar!) [Re: MadHatter333]
    #26874886 -

I was trying to clean up a moldy sample (more like an experiment than anything else..). That's a T6, mycelium (if it is mycelium in first place) looks weak. Still has some layer underneath it to... whatcha guys think? I want to learn so I appreciate your thoughts! :cheers:



Yes, I am running low on plates


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Re: AGAR ENVY! (Anything and All things agar!) [Re: shroomshine]
    #26874898 -

shroomshine said:
I was trying to clean up a moldy sample (more like an experiment than anything else..). That's a T6, mycelium (if it is mycelium in first place) looks weak. Still has some layer underneath it to... whatcha guys think? I want to learn so I appreciate your thoughts! :cheers:



Yes, I am running low on plates



Looks good.:thumbup:

P.Populinus:


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Question everything, it's very responsive.
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Re: AGAR ENVY! (Anything and All things agar!) [Re: Nichrome] * 1
    #26874925 -

Managed to achieve my smallest transfer to date. Recovering nicely... hopefully without the bacterial halo this time.



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Here Lies This Individual




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Re: AGAR ENVY! (Anything and All things agar!) [Re: The Dalcassian] * 1
    #26875550 -



Lion’s Mane finally visible to my old eyes, and Avery looking strong and beautiful.

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Shop: Myyco.com Isolated Cubensis Liquid Culture For Sale   North Spore Injection Grain Bag, North Spore Mushroom Grow Kits & Cultivation Supplies   Mushroom-Hut Substrate Mix

Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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