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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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Re: AGAR ENVY! (Anything and All things agar!) [Re: Je77Ce11ar] * 1
    #26687528 -

Je77Ce11ar said:
I recently ordered a pack of 500 plates off amazon, from a retailer I normally dont order from. The previous 500 i believe was from thomas scientific and came with a proof of sterilization sheet, but this one did not. Been experiencing at least 5-6 contamed plates per sleeve I've been pouring, whereas with my previous order I think I got 4-5 contam plates outta the whole 500. Anyone else have a similar problem with certain vendors??

Also, any tips on the best quality for price on a case of plates?


You can never go wrong with Celltreat with the grip ring for the win. I just go Amazon. If anybody knows a better deal though.....


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Crack's Tek's and Shit


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Re: AGAR ENVY! (Anything and All things agar!) [Re: Crackatoa]
    #26687546 -

Don't yell at me but...  LOL
But I get the same contam rate (10% too much I know )
whether I use PP from a sleeve or my freshly baked Corning Borosilicates.
so...:shrug:


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:sporedrop: GLOSSARY  :sporedrop: ACROMYMS!   :sporedrop: GETTING STARTED :sporedrop:

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Inthepit]
    #26687854 -

Try no-pouring your glass dishes for best results.


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How I do glass dishes

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Re: AGAR ENVY! (Anything and All things agar!) [Re: poisoned]
    #26687873 -

Thanks Poisoned, I think we had this conversation befoe...mebbe someone else.
But I could try again, I'm sure no pour would reduce my :horrified: contam rate!
Of course this time I could try PCing with the foiled stacks of plates above the water! :stars:
Also for extra Noobie credit, last time I wrapped a bit with cling wrap, so they stay together. But the cling wrap melted to the plates. I think I've finally scrubbed the last off of my beautiful plates. :heart:


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:sporedrop: GLOSSARY  :sporedrop: ACROMYMS!   :sporedrop: GETTING STARTED :sporedrop:

Edited by Inthepit (05/22/20 11:00 AM)

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Crackatoa]
    #26687918 -

Crackatoa said:
Je77Ce11ar said:
I recently ordered a pack of 500 plates off amazon, from a retailer I normally dont order from. The previous 500 i believe was from thomas scientific and came with a proof of sterilization sheet, but this one did not. Been experiencing at least 5-6 contamed plates per sleeve I've been pouring, whereas with my previous order I think I got 4-5 contam plates outta the whole 500. Anyone else have a similar problem with certain vendors??

Also, any tips on the best quality for price on a case of plates?


You can never go wrong with Celltreat with the grip ring for the win. I just go Amazon. If anybody knows a better deal though.....


YES! Those are by far my favorite plates. So strong and sturdy. I love those. Theyre more expensive than the budget plates you find for $50 or $60 a case, I think theyre like $120 a case, but theyre worth it imo. And they come in 10 pack sleeves instead of 20 or 25, so you dont gotta pour a whole 25 if you just need a few at that time.


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Trade List!
Current Projects!
Cheap Noob Cannabis Growing Guide!

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Thrill]
    #26690354 -

I'm about to pour some plates with some old grain soak water.

It's been in a gallon jug with a filter disc RTV'd to the top and it smells like it's probably started to ferment a bit.

Does anyone know if it's still ok to PC and pour up some plates?

Second question, I've had B+ and GT plates going, growing pretty fast on MEA.

I put them to rye grain and the temp has been between 68F-78F in the room. I guess I was expecting maybe 1 - 2 weeks for full colonization.

I'm at week 2 with maybe 30%, no contamination but seems slow.

would I be better off selecting for cultures that grow quickly on grain soak agar to have a better chance of fast colonizing times?

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Re: AGAR ENVY! (Anything and All things agar!) [Re: RyeHumor] * 1
    #26690404 -

I gave up with WBS grain water agar, contam rate was out 100% even with 60min PC; went back to MEA and no regrets, crystal clear with no contams!

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Re: AGAR ENVY! (Anything and All things agar!) [Re: oculodextro]
    #26690448 -

AA+ grows out slow and thick. Older dish seems to have started blueing. Is this good, bad, or neutral in the way of myc on agar?

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Re: AGAR ENVY! (Anything and All things agar!) [Re: StoneTroegs] * 1
    #26690470 -

That's bacterial as FUCK... Reason for the slow thick growth too... sorry dude


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🍄💚😎

Ask Caps! (My Q&A Thread)
Caps' Comprehensive Cultivation Tek List

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Caps McGee]
    #26690558 -

Interesting. Bacterial because it’s thick or bacterial because of the blueing?

How’s this look?

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Re: AGAR ENVY! (Anything and All things agar!) [Re: StoneTroegs]
    #26690563 -

All of the above... slow, thick, the bluing, the lack of characteristics resembling cubensis mycelium, the lack of uniformity in structure... I'm not sure that plate is cubensis even... its not healthy imo

Second look and consideration of the blue/green... you MIGHT have penicillium on your hands... are you fruiting these cultures yet?


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🍄💚😎

Ask Caps! (My Q&A Thread)
Caps' Comprehensive Cultivation Tek List

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Caps McGee]
    #26690573 -

Thanks. Def disappointing. Will play around with it just to see what happens.

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Caps McGee]
    #26690599 -

I put it to a few jars of WBS

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Re: AGAR ENVY! (Anything and All things agar!) [Re: StoneTroegs]
    #26690753 -

This would be a good one to make a log for. Good for new growers to see myc like that and the path it takes.


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:mushroom::mushroom::mushroom::mushroom::mushroom::mushroom::mushroom::mushroom::mushroom::mushroom:
:mushroom:                  :mushroom2:                :mushroom:
:mushroom:            :mushroom2::mushroom2::mushroom2:            :mushroom:
:mushroom:      :mushroom2::mushroom2::mushroom2::mushroom2::mushroom2:      :mushroom:
:mushroom::cookiemonster: BEHOLD :elmo::mushroom:
:mushroom::mushroom::mushroom::mushroom::mushroom::mushroom::mushroom::mushroom::mushroom::mushroom:

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Re: AGAR ENVY! (Anything and All things agar!) [Re: RyeHumor]
    #26690781 -

Anyone know if using lightly fermented GSW is ok for agar?

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Re: AGAR ENVY! (Anything and All things agar!) [Re: RyeHumor]
    #26690890 -

Seems any alcohol would evaporate during sterilization: give it a shot!


--------------------
🍄💚😎

Ask Caps! (My Q&A Thread)
Caps' Comprehensive Cultivation Tek List

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Caps McGee]
    #26690985 -

Finally just dumped a couple hundred old plates out so that I can stick em in the recycling.

I'm trying out my pp reusable petris along with my glass ones. Plan to just PC them and pour em after.

I'm not a fan of waiting for no pours to cool down inside the PC. Call me impatient (cause I am)


I hope they work out well. But I also just ordered a case of the 100mm Celltreat grip ring plates cause DAMN those suckers are convenient.

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Re: AGAR ENVY! (Anything and All things agar!) [Re: RyeHumor]
    #26691859 -

What are your objectives for each transfer?

Transfer 1 - transfer away from the unclean inoculate
Transfer 2 - transfer away from possible hidden contam

After you are sure it's clean from the first 2 transfers.....

How much do you let the mycelium grow out for each transfer afterwards, when do you start looking for sectoring/isolating, and when do you let it fill the whole plate to inoculate grain? Also, what do u do with clean plates from earlier transfers? Throw em?

Edited by ouuwee (05/24/20 06:03 AM)

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Re: AGAR ENVY! (Anything and All things agar!) [Re: ouuwee]
    #26691868 -

Seems like most people leave unused clean plates alone, and if they pin you can clone it. Never done it before, but I have some on a shelf now to see if they pin!

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Re: AGAR ENVY! (Anything and All things agar!) [Re: RyeHumor]
    #26691961 -

RyeHumor said:
I'm not a fan of waiting for no pours to cool down inside the PC. Call me impatient (cause I am)




Don't wait for them to cool in the PC.  Check out this no-pour tek. 
Josex' Guide to No-pour Agar

It's my absolute favorite.

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