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Crackatoa
Stranger in a strange land

Registered: 03/31/19
Posts: 5,406
Loc: Over by your Mama's house

Last seen: 11 months, 21 days

Re: AGAR ENVY! (Anything and All things agar!)

[Re: tedoro]
#26633970 - 04/28/20 09:51 AM (6 years, 4 months ago)

Yeah allot of people do. I'll go up 2% nutes when doing spores to agar. Some people may go higher.

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Crack's Tek's and Shit

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johnnyhitch1
Stranger

Registered: 04/16/20
Posts: 9
Last seen: 5 years, 9 months

Re: AGAR ENVY! (Anything and All things agar!)

[Re: tedoro]
#26633973 - 04/28/20 09:54 AM (6 years, 4 months ago)

Did some searching but couldn't find anything on whether diastatic malt powder is acceptable for MEA? (Have a bag leftover from baking)

Ingredients are Malt powder, Wheat powder and Dextrose

I would imagine the starch converting quality's from diastatic malt would only matter In a potato broth or PDA?

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tedoro
ToadStool Tender

Registered: 02/06/15
Posts: 2,274
Last seen: 3 months, 22 days

Re: AGAR ENVY! (Anything and All things agar!)

[Re: Crackatoa]
#26633987 - 04/28/20 10:01 AM (6 years, 4 months ago)

Crackatoa said:

Yeah allot of people do. I'll go up 2% nutes when doing spores to agar. Some people may go higher.

Anything else I can do to help germination?

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Deep pour soft agar plates-->bags of WBS-->Low Profile Monos
Clean spawn thread | Put a thermometer on your PC

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Crackatoa
Stranger in a strange land

Registered: 03/31/19
Posts: 5,406
Loc: Over by your Mama's house

Last seen: 11 months, 21 days

Re: AGAR ENVY! (Anything and All things agar!)

[Re: tedoro]
#26634055 - 04/28/20 10:28 AM (6 years, 4 months ago)

Are you trying to geminate a print or syringe?

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Crack's Tek's and Shit

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Inthepit
Aum Mani Padme Hum

Registered: 08/20/19
Posts: 1,742
Loc: Puerto Rico
Last seen: 1 year, 2 months

Re: AGAR ENVY! (Anything and All things agar!)

[Re: tedoro]
#26634066 - 04/28/20 10:32 AM (6 years, 4 months ago)

tedoro said:

Crackatoa said:

Yeah allot of people do. I'll go up 2% nutes when doing spores to agar. Some people may go higher.

Anything else I can do to help germination?

Well if you are using Oats, I like the idea here...
Primal's Experimental PE Grain Inoc>Agar Isolation Log (with pics and paragraphs :wink:

:wink:

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:sporedrop:

:sporedrop:

:sporedrop:

:sporedrop:

GETTING STARTED :sporedrop:

:sporedrop:

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RyeHumor
MasterProcrastinator

Registered: 02/21/20
Posts: 210
Loc: Yeast Of Knowhere

Re: AGAR ENVY! (Anything and All things agar!)

[Re: Inthepit]
#26634374 - 04/28/20 01:04 PM (6 years, 4 months ago)

Out of curiosity, anyone here ever tried using beet juice to dye their agar plates?

I'm going to give it a shot next round.

Nothing came up on a quick search though.

Most of my food coloring gets consumed pretty quickly so I wonder if it would be more persistent.

I know it's not necessary to agar work, but I enjoy the colors.

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Thrill
Regnarts

Registered: 02/05/11
Posts: 1,928
Loc: Beyond the Grey Sky

Last seen: 5 years, 11 months

Re: AGAR ENVY! (Anything and All things agar!)

[Re: RyeHumor]
#26634397 - 04/28/20 01:19 PM (6 years, 4 months ago)

I like the colors too lol. It beats looking at that ugly yellowish brown of uncolored agar. When I came back and saw all the bright colored agar everyone was using I was like :Awemazing:

:Awemazing:

Wow, Im in the future lmao

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ModularMind
M.P.F.

Registered: 02/09/10
Posts: 8,784

Re: AGAR ENVY! (Anything and All things agar!)

[Re: Thrill]
#26634417 - 04/28/20 01:29 PM (6 years, 4 months ago)

Thrill said:

When I came back and saw all the bright colored agar everyone was using I was like Wow, Im in the future lmao

:juggle:

:tinfoil:

:juggle:

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novictimnocrime
Cruisin’ for a bruisin’

Registered: 06/27/11
Posts: 158
Loc:

Last seen: 2 years, 4 months

Re: AGAR ENVY! (Anything and All things agar!)

[Re: RyeHumor]
#26634420 - 04/28/20 01:30 PM (6 years, 4 months ago)

http://

Pan Jam 4 days after MS inoculation from print, with disposable loop in SAB. Shizz is growing quick!

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Camera93
We got dicks like Jesus

Registered: 08/15/18
Posts: 3,588
Last seen: 8 hours, 13 minutes

Re: AGAR ENVY! (Anything and All things agar!)

[Re: novictimnocrime]
#26634424 - 04/28/20 01:32 PM (6 years, 4 months ago)

novictimnocrime said:

http://

Pan Jam 4 days after MS inoculation from print, with disposable loop in SAB. Shizz is growing quick!

Awesome! check out the official pan thread if you havent yet for some input on where to transfer from. They seem to prefer a certain type of growth to avoid overlay

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Whatever you decide won’t really impact our survival
Close your eyes, and do the best that you can

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A.k.a
Stranger

Registered: 10/27/19
Posts: 18,216
Loc: Gaming the system
Last seen: 15 hours, 21 minutes

Re: AGAR ENVY! (Anything and All things agar!)

[Re: Camera93]
#26634430 - 04/28/20 01:35 PM (6 years, 4 months ago)

Thin and wispy, no super puffy myc.

You’ll see as it grows out, some of my germ plate myc is pushed up against the lid it’s so puffy.

You want some similar to this, minus the rampant bacteria.

I think my favorite part of pans is how fast they are. It’s like growing cubes on fast forward. So odd spawning a tray and coming back the next day to a totally colonized surface.

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Edited by A.k.a (04/28/20 01:39 PM)

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tedoro
ToadStool Tender

Registered: 02/06/15
Posts: 2,274
Last seen: 3 months, 22 days

Re: AGAR ENVY! (Anything and All things agar!)

[Re: Crackatoa]
#26634441 - 04/28/20 01:43 PM (6 years, 4 months ago)

Crackatoa said:

Are you trying to geminate a print or syringe?

Print

--------------------
--------------------
Deep pour soft agar plates-->bags of WBS-->Low Profile Monos
Clean spawn thread | Put a thermometer on your PC

Extras:

novictimnocrime
Cruisin’ for a bruisin’

Registered: 06/27/11
Posts: 158
Loc:

Last seen: 2 years, 4 months

Re: AGAR ENVY! (Anything and All things agar!)

[Re: tedoro]
#26634464 - 04/28/20 01:56 PM (6 years, 4 months ago)

thanks, yes I’ve been reading a lot and i can actually already see some of what i believe is the right type of growth (thin, wispy and kinda sprawling) but it’s very small and i’d like to see what happens over the next couple days. Yeah the growth seems crazy fast compared to cubes this is my 2nd go around with Pans so im learning ✌️

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poisoned
untitled

Registered: 04/17/13
Posts: 1,738
Loc: Yurop
Last seen: 3 years, 10 months

Re: AGAR ENVY! (Anything and All things agar!)

[Re: RyeHumor]
#26634793 - 04/28/20 04:20 PM (6 years, 4 months ago)

RyeHumor said:

Out of curiosity, anyone here ever tried using beet juice to dye their agar plates?

I'm going to give it a shot next round.

Nothing came up on a quick search though.

Most of my food coloring gets consumed pretty quickly so I wonder if it would be more persistent.

I know it's not necessary to agar work, but I enjoy the colors.

It would probably work, but it's probably going to have a lot of sediment.

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How I do glass dishes

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RyeHumor
MasterProcrastinator

Registered: 02/21/20
Posts: 210
Loc: Yeast Of Knowhere

Re: AGAR ENVY! (Anything and All things agar!)

1
#26635017 - 04/28/20 06:08 PM (6 years, 4 months ago)

Yeah that's why I figure I'm going to run it through a coffee filter before adding the agar.

I might just try using water from boiling beets as the nutes and the color.

worst thing that could happen is it doesn't work :shrug:

:shrug:

Best case I get to boil my beets and eat them too.

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Dephect

Registered: 08/10/07
Posts: 1,779

Re: AGAR ENVY! (Anything and All things agar!)

[Re: RyeHumor]
#26635083 - 04/28/20 06:33 PM (6 years, 4 months ago)

Got some Nepal MS plates and some Honduras clone plates

Just transferred both to LC at the beginning of the week.

Nepal

Honduras clone

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foodguy
MycoMealMaker

Registered: 01/05/17
Posts: 106
Last seen: 5 years, 6 months

Re: AGAR ENVY! (Anything and All things agar!)

[Re: RyeHumor]
#26635095 - 04/28/20 06:38 PM (6 years, 4 months ago)

Just getting my clean space and hood set back up. Was planning on pulling a print out, then I found these 2 plates from a clone from last october in my fridge...

They both look pretty clean, but they've grown out way farther that what I'd want to use for a typical transfer. Any recommendations on trying to get a transfer and grow this line back out, or just need to wait for them to pin maybe?

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Tormato
Chairman of the Bored

Registered: 07/01/17
Posts: 6,147
Last seen: 28 days, 14 hours

Re: AGAR ENVY! (Anything and All things agar!)

[Re: foodguy]
#26635251 - 04/28/20 07:57 PM (6 years, 4 months ago)

foodguy said:

Just getting my clean space and hood set back up. Was planning on pulling a print out, then I found these 2 plates from a clone from last october in my fridge...

They both look pretty clean, but they've grown out way farther that what I'd want to use for a typical transfer. Any recommendations on trying to get a transfer and grow this line back out, or just need to wait for them to pin maybe?

I'd still transfer from those...they look ok :thumbup:

:thumbup:

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ichugwindex
Dex

Registered: 06/04/16
Posts: 4,613
Loc: ¯\_(ツ)_/¯
Last seen: 1 year, 11 months

Re: AGAR ENVY! (Anything and All things agar!)

[Re: Dephect]
#26635317 - 04/28/20 08:25 PM (6 years, 4 months ago)

Dephect said:

Got some Nepal MS plates and some Honduras clone plates

Just transferred both to LC at the beginning of the week.

Nepal

Honduras clone

Woah dude I wish I could do that.

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Only hope can give rise to the emotion we call despair. But it is nearly impossible for a man to try to live without hope, so I guess that leaves Man no choice but to walk around with despair as his companion.

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citrinopileatus
Newcomer

Registered: 07/06/18
Posts: 23
Loc: Midwest USA
Last seen: 2 years, 7 months

Re: AGAR ENVY! (Anything and All things agar!)

[Re: Tormato]
#26635467 - 04/28/20 09:37 PM (6 years, 4 months ago)

Got some alright looking sectors from my first transfer off a print. Including some growing from a chunk of agar I accidentally dropped back on the plate it came from :cool:

:cool:

Also feat. a spot of bacteria. Need to improve my sterile procedure I guess

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