mushboy said:

[I like doing a splash per jar. Splash being a technical term for 10ish ml.

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A.k.a said:

Seems like you’d have to underhydrate the grains or somehow strain the myc from the broth.

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Pour a splash and put the cap back on

verum subsequentis said:

I've put 120 ccs into a bag before. If you prep the grain dry you can add a shitload of liquid. Generally i just do a "splash" like mushboy said. Getting ready to test out a couple LCs myself.

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ShaperDreaming said:

sandman420 said:

Sure you can keep reusing it but there's an increased chance that some bacteria could grow on your ports or in the jar from a dirty operation and have time to grow. If you are using it all at once at least it won't all go bad from a single mistake. Just better chances that's all. LC can be a prickly whore when it goes wrong. I personally size my LC jars to have exactly what I need for one "batch" and it all works out good.

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I recently had a 75% colonized LC of 400ml, it hadn't really done anything in a while so I decided to unscrew the media bottle 1/4 turn in case I was suffocating it (I have a tendency to keep my unmodded lids a little on the tight side unfortunately due to paranoia). 3 days later I noticed mold around the inner rim It'd been growing out for almost 4 weeks w/o issue and one little 1/4 twist and BAM green.



Yeah, prickly whore is an understatement.

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mushboy said:

getting contams around the rim of a jar is from being sloppy

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wipe your rim better than you would your ass for best results

A.k.a said:

Say you had unlimited lc and just needed a quart colonized ASAP, how would you do it??

Seems like you’d have to underhydrate the grains or somehow strain the myc from the broth.

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trubblesome said:

A.k.a said:

Say you had unlimited lc and just needed a quart colonized ASAP, how would you do it??

Seems like you’d have to underhydrate the grains or somehow strain the myc from the broth.

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i did this in my LAGM. I let the LC settle, and decanted the liquid off the top, then I dumped the rest (big boogers of myc, it was great) in to a jar of oats I prepped purposefully dry. I weighed the grains dry and after prep to be sure, used a shorter boil, and a longer dry time before PC, as well as leaving about half the grains in the still hot pot to dry out even more. dry vs wet weight indicated about 60% added hydration (not the real numbers but for instance 160g wet to 100g dry) It worked much better than my other method, where I accidentally dumped about 60 ml or so right in to a jar of grains that had been sitting around a while drying out.

with both I was scared at first by the pool of LC at the bottom but a good shake, letting it settle again, and then one more shake did the trick.

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Quote:

both my LC test plates came up clean, so yesterday I took the remainder of the LC, decanted most of the liquid off the top, and dumped about 15 ml of nicely developed snot balls of liquid myc on to two new jars, one for each LC. then I took a sample from the remainder and observed it under the scope just to verify it was still clean.

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A.k.a said:

That’s exactly what I’m gonna try, pouring off as much as I can first.

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MLPismyOPSEC said:

A.k.a said:

That’s exactly what I’m gonna try, pouring off as much as I can first.

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Is this SOP? I did this, but it feels wasteful. I'm going to get a stirplate to hopefully avoid this in the future. I swirled hard towards the end but it wasn't enough to break up the myc at that point, and i didn't want to shake it as if it were a grain jar.

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